New SPB stars in the field of the young open cluster NGC 2244 discovered by the MOST photometric satellite

During two weeks of nearly continuous optical photometry of the young open cluster NGC 2244 obtained by the MOST satellite, we discovered two new SPB stars, GSC 00154-00785 and GSC 00154-01871. We present frequency analyses of the MOST light curves of these stars, which reveal two oscillation frequencies (0.61 and 0.71 c/d) in GSC 00154-00785 and two (0.40 and 0.51 c/d) in GSC 00154-01871. These frequency ranges are consistent with g-modes of $\ell \leq 2$ excited in models of main-sequence or pre-main-sequence (PMS) stars of masses 4.5 - 5 $M_{\odot}$ and solar composition $(X, Z)= (0.7, 0.02)$. Published proper motion measurements and radial velocities are insufficient to establish unambiguously cluster membership for these two stars. However, the PMS models which fit best their eigenspectra have ages consistent with NGC 2244. If cluster membership can be confirmed, these would be the first known PMS SPB stars, and would open a new window on testing asteroseismically the interior structures of PMS stars.Comment: accepted for publication in MNRA

HD 174884: a strongly eccentric, short-period early-type binary system discovered by CoRoT

Accurate photometric CoRoT space observations of a secondary seismological target, HD 174884, led to the discovery that this star is an astrophysically important double-lined eclipsing spectroscopic binary in an eccentric orbit (e of about 0.3), unusual for its short (3.65705d) orbital period. The high eccentricity, coupled with the orientation of the binary orbit in space, explains the very unusual observed light curve with strongly unequal primary and secondary eclipses having the depth ratio of 1-to-100 in the CoRoT 'seismo' passband. Without the high accuracy of the CoRoT photometry, the secondary eclipse, 1.5 mmag deep, would have gone unnoticed. A spectroscopic follow-up program provided 45 high dispersion spectra. The analysis of the CoRoT light curve was performed with an adapted version of PHOEBE that supports CoRoT passbands. The final solution was obtained by simultaneous fitting of the light and the radial velocity curves. Individual star spectra were derived by spectrum disentangling. The uncertainties of the fit were derived by bootstrap resampling and the solution uniqueness was tested by heuristic scanning. The results provide a consistent picture of the system composed of two late B stars. The Fourier analysis of the light curve fit residuals yields two components, with orbital frequency multiples and an amplitude of about 0.1 mmag, which are tentatively interpreted as tidally induced pulsations. An extensive comparison with theoretical models is carried out by means of the Levenberg-Marquardt minimization technique and the discrepancy between models and the derived parameters is discussed. The best fitting models yield a young system age of 125 million years which is consistent with the eccentric orbit and synchronous component rotation at periastron.Comment: 15 pages, 12 figures. Accepted for publication by A&

A spectroscopic study of southern (candidate) gamma Doradus stars. II. Detailed abundance analysis and fundamental parameters

The gamma Doradus stars are a recent class of variable main sequence F-type stars located on the red edge of the Cepheid instability strip. They pulsate in gravity modes, and this makes them particularly interesting for detailed asteroseismic analysis, which can provide fundamental knowledge of properties near the convective cores of intermediate-mass main sequence stars. To improve current understanding of gamma Dor stars through theoretical modelling, additional constraints are needed. Our aim is to estimate the fundamental atmospheric parameters and determine the chemical composition of these stars. Detailed analyses of single stars have previously suggested links to Am and lambda Bootis stars, so we wish to explore this interesting connection between chemical peculiarity and pulsation. We have analysed a sample of gamma Dor stars for the first time, including nine bona fide and three candidate members of the class. We determined the fundamental atmospheric parameters and compared the abundance pattern with other A-type stars. We used the semi-automatic software package VWA for the analysis. This code relies on the calculation of synthetic spectra and thus takes line-blending into account. This is important because of the fast rotation in some of the sample stars, and we made a thorough analysis of how VWA performs when increasing vsini. We obtained good results in agreement with previously derived fundamental parameters and abundances in a few selected reference stars with properties similar to the gamma Dor stars. We find that the abundance pattern in the gamma Dor stars is not distinct from the constant A- and F-type stars we analysed.Comment: 13 pages, accepted by A&

Multiyear monitoring (2007-2013) of flat-tailed horned lizards (Phrynosoma mcallii)

Monitoring programs for species of conservation concern are notoriously flawed. Notably, many monitoring programs do not establish trigger points or a level of decline in population size that will result in management action. Here we report on the monitoring program for the Flat-tailed Horned Lizard (Phrynosoma mcallii) that has been established across its range throughout the United States by the Flat-tailed Horned Lizard Interagency Coordinating Committee (FTHL ICC). Impor- tantly, we examine whether a trigger point of 30% decline was detected in these populations. Between 2007 and 2013, we detected 955 P. mcallii on 2,714 occupancy surveys and captured 715 individuals on 1,861 demographic surveys. Occupancy surveys have demonstrated that the species occurs through- out the management areas and occupancy estimates range from 0.25–0.89. Demographic surveys have demonstrated that population trends over time are correlated across all management areas; however, they are probably driven by factors at smaller geographic scales. During the study no population decline \u3e 30% was detected after accounting for natural and stochastic fluctuations. Continued mon- itoring is called for to gain a greater understanding of what is driving the trends in populations both range-wide and at the scale of management areas

Discovery and analysis of p-mode and g-mode oscillations in the A-type primary of the eccentric binary HD 209295

We have discovered both intermediate-order gravity mode and low-order pressure mode pulsation in the same star, HD 209295. It is therefore both a Gamma Doradus and a Delta Scuti star, which makes it the first pulsating star to be a member of two classes. The star is a single-lined spectroscopic binary with an orbital period of 3.10575 d and an eccentricity of 0.352. Weak pulsational signals are found in both the radial velocity and line-profile variations, allowing us to show that the two highest-amplitude Gamma Doradus pulsation modes are consistent with l=1 and |m|=1. In our 280 h of BVI multi-site photometry we detected ten frequencies in the light variations, one in the Delta Scuti regime and nine in the Gamma Doradus domain. Five of the Gamma Doradus frequencies are exact integer multiples of the orbital frequency. This observation leads us to suspect they are tidally excited. Results of theoretical modeling (stability analysis, tidal excitation) were consistent with the observations. We could not detect the secondary component of the system in infrared photometry, suggesting that it may not be a main-sequence star. Archival data of HD 209295 show a strong ultraviolet excess, the origin of which is not known. The orbit of the primary is consistent with a secondary mass of M > 1.04 Msun indicative of a neutron star or a white dwarf companion.Comment: 18 pages, 18 figures, accepted for publication in MNRAS, shortened abstrac

Bayesian inference reveals positive but subtle effects of experimental fishery closures on marine predator demographics

Global forage-fish landings are increasing, with potentially grave consequences for marine ecosystems. Predators of forage fish may be influenced by this harvest, but the nature of these effects is contentious. Experimental fishery manipulations offer the best solution to quantify population-level impacts, but are rare. We used Bayesian inference to examine changes in chick survival, body condition and population growth rate of endangered African penguins Spheniscus demersus in response to 8 years of alternating time-area closures around two pairs of colonies. Our results demonstrate that fishing closures improved chick survival and condition, after controlling for changing prey availability. However, this effect was inconsistent across sites and years, highlighting the difficultly of assessing management interventions in marine ecosystems. Nevertheless, modelled increases in population growth rates exceeded 1% at one colony; i.e. the threshold considered biologically meaningful by fisheries management in South Africa. Fishing closures evidently can improve the population trend of a forage-fish-dependent predator-we therefore recommend they continue in South Africa and support their application elsewhere. However, detecting demographic gains for mobile marine predators from small no-take zones requires experimental time frames and scales that will often exceed those desired by decision makers

Cell-based tissue engineering strategies used in the clinical repair of articular cartilage

One of the most important issues facing cartilage tissue engineering is the inability to move technologies into the clinic. Despite the multitude of review articles on the paradigm of biomaterials, signals, and cells, it is reported that 90% of new drugs that advance past animal studies fail clinical trials (1). The intent of this review is to provide readers with an understanding of the scientific details of tissue engineered cartilage products that have demonstrated a certain level of efficacy in humans, so that newer technologies may be developed upon this foundation. Compared to existing treatments, such as microfracture or autologous chondrocyte implantation, a tissue engineered product can potentially provide more consistent clinical results in forming hyaline repair tissue and in filling the entirety of the defect. The various tissue engineering strategies (e.g., cell expansion, scaffold material, media formulations, biomimetic stimuli, etc.) used in forming these products, as collected from published literature, company websites, and relevant patents, are critically discussed. The authors note that many details about these products remain proprietary, not all information is made public, and that advancements to the products are continuously made. Nevertheless, by fully understanding the design and production processes of these emerging technologies, one can gain tremendous insight into how to best use them and also how to design the next generation of tissue engineered cartilage products

N-3 Polyunsaturated Fatty Acids and the Resolution of Neuroinflammation

In the past few decades, as a result of their anti-inflammatory properties, n-3 long chain polyunsaturated fatty acids (n-3 LC-PUFAs), have gained greater importance in the regulation of inflammation, especially in the central nervous system (in this case known as neuroinflammation). If sustained, neuroinflammation is a common denominator of neurological disorders, including Alzheimer's disease and major depression, and of aging. Hence, limiting neuroinflammation is a real strategy for neuroinflammatory disease therapy and treatment. Recent data show that n-3 LC-PUFAs exert anti-inflammatory properties in part through the synthesis of specialized pro-resolving mediators (SPMs) such as resolvins, maresins and protectins. These SPMs are crucially involved in the resolution of inflammation. They could be good candidates to resolve brain inflammation and to contribute to neuroprotective functions and could lead to novel therapeutics for brain inflammatory diseases. This review presents an overview 1) of brain n-3 LC-PUFAs as precursors of SPMs with an emphasis on the effect of n-3 PUFAs on neuroinflammation, 2) of the formation and action of SPMs in the brain and their biological roles, and the possible regulation of their synthesis by environmental factors such as inflammation and nutrition and, in particular, PUFA consumption

Identification of genetic variants associated with Huntington's disease progression: a genome-wide association study

Background Huntington's disease is caused by a CAG repeat expansion in the huntingtin gene, HTT. Age at onset has been used as a quantitative phenotype in genetic analysis looking for Huntington's disease modifiers, but is hard to define and not always available. Therefore, we aimed to generate a novel measure of disease progression and to identify genetic markers associated with this progression measure. Methods We generated a progression score on the basis of principal component analysis of prospectively acquired longitudinal changes in motor, cognitive, and imaging measures in the 218 indivduals in the TRACK-HD cohort of Huntington's disease gene mutation carriers (data collected 2008–11). We generated a parallel progression score using data from 1773 previously genotyped participants from the European Huntington's Disease Network REGISTRY study of Huntington's disease mutation carriers (data collected 2003–13). We did a genome-wide association analyses in terms of progression for 216 TRACK-HD participants and 1773 REGISTRY participants, then a meta-analysis of these results was undertaken. Findings Longitudinal motor, cognitive, and imaging scores were correlated with each other in TRACK-HD participants, justifying use of a single, cross-domain measure of disease progression in both studies. The TRACK-HD and REGISTRY progression measures were correlated with each other (r=0·674), and with age at onset (TRACK-HD, r=0·315; REGISTRY, r=0·234). The meta-analysis of progression in TRACK-HD and REGISTRY gave a genome-wide significant signal (p=1·12 × 10−10) on chromosome 5 spanning three genes: MSH3, DHFR, and MTRNR2L2. The genes in this locus were associated with progression in TRACK-HD (MSH3 p=2·94 × 10−8 DHFR p=8·37 × 10−7 MTRNR2L2 p=2·15 × 10−9) and to a lesser extent in REGISTRY (MSH3 p=9·36 × 10−4 DHFR p=8·45 × 10−4 MTRNR2L2 p=1·20 × 10−3). The lead single nucleotide polymorphism (SNP) in TRACK-HD (rs557874766) was genome-wide significant in the meta-analysis (p=1·58 × 10−8), and encodes an aminoacid change (Pro67Ala) in MSH3. In TRACK-HD, each copy of the minor allele at this SNP was associated with a 0·4 units per year (95% CI 0·16–0·66) reduction in the rate of change of the Unified Huntington's Disease Rating Scale (UHDRS) Total Motor Score, and a reduction of 0·12 units per year (95% CI 0·06–0·18) in the rate of change of UHDRS Total Functional Capacity score. These associations remained significant after adjusting for age of onset. Interpretation The multidomain progression measure in TRACK-HD was associated with a functional variant that was genome-wide significant in our meta-analysis. The association in only 216 participants implies that the progression measure is a sensitive reflection of disease burden, that the effect size at this locus is large, or both. Knockout of Msh3 reduces somatic expansion in Huntington's disease mouse models, suggesting this mechanism as an area for future therapeutic investigation

Over 25 Years of Partnering to Conserve Chiricahua Leopard Frogs (<i>Rana chiricahuensis</i>) in Arizona, Combining Ex Situ and In Situ Strategies

The Phoenix Zoo has partnered with US Fish and Wildlife Service, Arizona Game and Fish Department, US Forest Service, and other organizations for more than 25 years to help recover Chiricahua leopard frogs (Rana [=Lithobates] chiricahuensis) in Arizona, USA. This federally threatened species faces declines due to habitat loss and degradation, long-term drought, disease, and invasive species. Over 26,000 larvae, froglets, and adults, as well as 26 egg masses produced by adults held at the Phoenix Zoo have been released to the wild, augmenting and/or re-establishing wild populations. Chiricahua leopard frog-occupied sites in Arizona have increased from 38 in 2007, when the species’ recovery plan was published, to a high of 155 in the last five years, as a result of ex situ and in situ conservation efforts. As one of the longest-running programs of its kind in the United States, communication among partners has been key to sustaining it. Recovery strategies and complex decisions are made as a team and we have worked through numerous management challenges together. Though Chiricahua leopard frogs still face significant threats and a long road to recovery, this program serves as a strong example of the positive effects of conservation partnerships for native wildlife