Longitudinal Changes in Response to a Cycle-Run Field Test of Young Male National "Talent identification" and Senior Elite Triathlon Squads.

This study investigated the changes in cardiorespiratory response and running performance of 9 male ?Talent Identification? (TID) and 6 male Senior Elite (SE) Spanish National Squad triathletes during a specific cycle-run test. The TID and SE triathletes (initial age 15.2±0.7 vs. 23.8±5.6 years, p=0.03; tests through the competitive period and the preparatory period, respectively, of two consecutive seasons: Test 1 was an incremental cycle test to determine the ventilatory threshold (Thvent); Test 2 (C-R) was 30 min constant load cycling at the Thvent power output followed by a 3-km time trial run; and Test 3 (R) was an isolated 3-km time trial control run, in randomized counterbalanced order. In both seasons the time required to complete the C-R 3-km run was greater than for R in TID (11:09±00:24 vs. 10:45±00:16 min:ss, pmenor que 0.01; and 10:24±00:22 vs. 10:04±00:14, p=0.006, for season 2005/06 and 2006/07, respectively) and SE (10:15±00:19 vs. 09:45±00:30, pmenor que 0.001 and 09:51±00:26 vs. 09:46±00:06, p= 0.02 for season 2005/06 and 2006/07, respectively). Compared to the first season, completion of the time trial run was faster in the second season (6.6%, pmenor que 0.01 and 6.4%, pmenor que 0.01, for C-R and R test, respectively) only in TID. Changes in post-cycling run performance were accompanied by changes in pacing strategy but only slight or non-significant changes in the cardiorespiratory response. Thus, the negative effect of cycling on performance may persist, independently of the period, over two consecutive seasons in TID and SE triathletes; however A improvements over time suggests that monitoring running pacing strategy after cycling may be a useful tool to control performance and training adaptations in TID. O2max 77.0±5.6 vs. 77.8±3.6 mL·kg-1·min-1, NS) underwent three TE D EP C

Influencia de la propuesta didáctica las maravillas de apata, en el logro de competencias comunicativas de expresión y comprensión oral en los niños y niñas de cuatro años de la Institución Educativa Inicial N° 152 - Apata - Región Junín – 2012

Las investigaciones mostraban en distintos contextos y actividades, niveles no esperados en el desarrollo de la competencia se comunica oralmente en lengua materna, las que al ser analizadas, permitían concluir que ello era reflejo de la practica pedagógica implementada en la Institución Educativa, en función al desarrollo de estrategias metodológicas y uso de materiales educativos con imágenes y realidades de situaciones distintas a la que viven los estudiantes, por ello surgió la necesidad de elaborar una propuesta didáctica denominada Las Maravillas de Apata, con el propósito de fortalecer e impulsar el desarrollo de dicha competencia. La investigación tiene un estudio de tipo aplicado, de un pre test y post test con un solo grupo, para el proceso de recojo de la información se utilizó como técnica la observación teniendo como instrumento la ficha de observación que contiene 30 ítems, La aplicación de la propuesta didáctica Las Maravillas de Apata, ha permitido corroborar que las actividades de aprendizaje diseñadas e implementadas durante el proceso de aplicación de la investigación han ofrecido resultados óptimos que permiten concluir que los contenidos de la cultura del contexto impulsan de manera sobresaliente el desarrollo no solo de la competencia se comunica oralmente en lengua materna, tema de la investigación, sino que a la par se han ido observado el desarrollo muy marcado de la competencia de personal social y matemática, que serán motivo de otra investigación.The investigation showed in different contexts and activities unexpected levels in the development of the competence communicates orally in the mother tongue, which when analyzed, allowed to conclude that this was a reflection of the pedagogical practice implemented in the Educational Institution, depending on the development of methodological strategies and use of educational materials with images and realities of situations other than those in which students live, for this reason the need arose to elaborate a didactic proposal called Las Maravillas de Apata, with the purpose of strengthening and promoting the development of said competence. The research has an applied-type study, of a pre-test and post-test with a single group, for the process of collecting the information, observation was used as an instrument, having as an instrument the observation sheet that contains 30 items, The application of the didactic proposal Las Maravillas de Apata, has corroborated that the learning activities designed and implemented during the process of applying the research have offered optimal results that allow to conclude that the contents of the culture of the context promote in an outstanding way the development not only of the competence is communicated orally in the mother tongue, the subject of the research, but at the same time the very marked development of the competence of social and mathematical personnel has been observed, which will be the subject of another research

Influencia de la propuesta didáctica las maravillas de Apata, en el logro de la competencia actúa y piensa matemáticamente en situaciones de cantidad, en los estudiantes de cinco años de la Institución Educativa Inicial N° 152 Apata de la Región Junín 2019

El objetivo de este trabajo de investigación es determinar la influencia de la propuesta didáctica las maravillas de Apata, en el logro de la competencia actúa y piensa matemáticamente en situaciones de cantidad, en los estudiantes de cinco años de la Institución Educativa Inicial N° 152 Apata de la Región Junín, 2019. Esta investigación es de enfoque cuantitativo, tipo de una investigación aplicada, de diseño cuasiexperimental. La muestra seleccionada es no probabilística intencional con dos grupos intactos, es decir, 18 niños del grupo experimental y 16 del control. A ambos se les aplicó el instrumento la lista de cotejo en pretest y postest para medir la variable dependiente, previamente validado por juicio de expertos y con una confiabilidad Kuder Richarson = 0,770. Después de la aplicación de la propuesta didáctica los resultados descriptivos indican que el GE tienen mejores condiciones que el GC en el postest siendo la media mayor en el GE (24,56) en referencia al GC (16,94); asimismo, una mediana es distante 24 puntos en el GE y 16 puntos en el GC. Los resultados de la estadística inferencial demostraron que Existen diferencias significativas entre los resultados que obtuvieron los niños del grupo experimental y los del grupo de control en un (z = -4,508, p < 0.05) a favor del grupo experimental. En conclusión, Existe influencia significativa de la propuesta didáctica las maravillas de Apata, en el logro de la competencia actúa y piensa matemáticamente en situaciones de cantidad, en los estudiantes de cinco años de la Institución Educativa Inicial N° 152 Apata de la Región Junín, 2019.The objective of this research work is to determine the influence of the didactic proposal the wonders of Apata, in the achievement of the competence acts and thinks mathematically in situations of quantity, in the five-year-old students of the Initial Educational Institution No. 152 Apata of the Junín Region, 2019. This research is of a quantitative approach, a type of applied research, of a quasi-experimental design. The selected sample is intentional non-probabilistic with two intact groups, that is, 18 children from the experimental group and 16 from the control group. The pretest and posttest checklist instrument was applied to both to measure the dependent variable, previously validated by expert judgment and with a Kuder Richarson reliability = 0.770. After the application of the didactic proposal, the descriptive results indicate that the GE have better conditions than the GC in the post-test, with the highest mean in the GE (24.56) in reference to the CG (16.94); likewise, a median is distant 24 points in the GE and 16 points in the CG. The results of the inferential statistics showed that there are significant differences between the results obtained by the children of the experimental group and those of the control group in a (z = -4,508, p < 0.05) in favor of the experimental group. In conclusion, there is a significant influence of the didactic proposal the wonders of Apata, in the achievement of the competence acts and thinks mathematically in situations of quantity, in the five-year-old students of the Initial Educational Institution No. 152 Apata of the Junín Region, 2019.ChosicaMetodología y didácticaEscuela de Posgrad

PHYSICAL SELF-CONCEPT OF MEXICAN ATHLETE AND NON-ATHLETE TEENAGERS

The purpose of this study consists of outlining the differences and similarities between elementary and junior-high school Mexican students’ physical self-concept based on the variables of those who practice a sport on a regular basis and those who do not do. A total sample of 596 male students, between the ages of 11-16 participated in this study; 270 of them practice a sport and participate regularly in tournaments or competitions. A quantitative approach with a descriptive and transversal survey design was used. All the participants completed the Physical Self-Description Questionnaire. The results of the one-way multivariate analysis of variance, followed by the oneway univariate analyses of variance, show that students who do not participate in sports regularly are the ones who obtained lower scores on the coordination, activity, sports competence, appearance, strength, flexibility, endurance, global physical self-concept, and global self-esteem subscales. However, in the health subscale, statistically significant differences were not found. Further research should seek these findings in larger samples

Estrategia didáctica para la baja comprensión y producción de la lectoescritura en los estudiantes de ciclo cuatro de la jornada nocturna de la I.E.D Nueva Constitución

El presente trabajo titulado Estrategia didáctica para la baja comprensión y producción de la lectoescritura en los estudiantes de ciclo cuatro de la jornada nocturna de la I.E.D Nueva Constitución se determinó con la realización de una prueba diagnóstica en la cual se les solicitó redactar a los estudiantes un párrafo de índole reflexiva en el que se viera evidenciada su opinión o postura social acerca de las diversas realidades que ha conformado la coyuntura sociopolítica actual en Colombia; esto con el fin de identificar posibles falencias de orden deductivo, cohesivo y ortográfico. Por medio de la literatura infantil se buscó la manera más optima de acercar a la población andragógica a los hábitos lectoescritores usando los cuentos de Evelio Rosero. Este autor nos permitía establecer situaciones cotidianas en un contexto educativo donde todo el mundo podía verse identificado, lo cual fue de gran ayuda al compartir experiencias y anécdotas tanto con los más jóvenes como con los más adultos del grupo correspondiente al manejar una literatura transparente en donde no se buscaba idealizar la infancia sino mostrarla de una manera más realista. Gracias a esto se pudo introducir el concepto de otredad como una alternativa de comprender y sensibilizarse con los problemas que normalmente lidia el estudiante en grupos sociales como escuela, trabajo y amigos; lo anterior evidenció una mayor motivación por parte de los estudiantes para la realización de trabajos escritos mejorando el hábito lector.Universidad Libre - Ciencias de la Educación - Licenciatura en Español y Lenguas ExtranjerasThe night shift of Colegio Nueva Constitución consists of a population whose age range is diverse; in several groups it can be found teenagers, adults, and senior citizens. This variable might be caused by several economic, social, and personal factors which at the same time can be a limitation for the correct development of the literary skills in the student. For this reason, the project Evelio Rosero´s literature for the development of the otherness in the literary skills process of the students in the third cycle night shift of the I.E.D Nueva Constitucion was determined by means of a diagnostic test in which students were asked to write a reflective paragraph in which their opinion or social stance on the different realities that have shaped the current socio-political situation in Colombia was made evident this is to identify possible deductive, cohesive, and orthographic shortcomings. This allowed us to determine a problematic situation, and consequently to formulate a problem question, which directs the task of this research: to design a didactic strategy based on the literature of Evelio Rosero's otherness to improve the reading and writing process in the students of cycle three of the evening classes of the school I.E.D Nueva Constituci

Relationship between resilience, optimism, and burnout in Pan-American athletes

Aim: A series of knowledge has been developed on burnout syndrome in the sports context that has allowed to generate a solid theoretical structure that requires new contributions to delve into those aspects that have been less addressed, as is the case of optimism and resilience when it comes to linking them to the syndrome in top-performance athletes. For this purpose, the Burnout Inventory for Athletes (IBD-r), the Resilience Scale adapted to Spanish, and the Life Orientation Scale-Revised (LOT-R) for optimism were used. Methods: From this perspective, the study was approached with 121 Uruguayan athletes who participated in the last Pan-American Games, assuming practically all the subjects who have this level of sport (142) considering gender and type of sport. Results: The results indicate that 67% of the sample presents burnout symptoms for the emotional exhaustion dimension, and moderate resilience and optimism. Although there are no significant differences attributable to gender or type of sport, this contribution allows us to continue focusing on future work and further analysis. The type of sport presented statistically significant differences in relation to the personal competence dimension and the type of sport. Conclusion: Resilience and optimism obtained a clear influence on the occurrence of burnout, in a statistically negative sense, showing themselves as interesting prevention strategies for future lines of research, where it is essential to design interventions that teach emotional skills to manage adversity and prevent burnout. Copyrigh

Extracellular vesicles from pluripotent stem cell-derived mesenchymal stem cells acquire a stromal modulatory proteomic pattern during differentiation

Mesenchymal stem/stromal cells (MSCs) obtained from pluripotent stem cells (PSCs) constitute an interesting alternative to classical MSCs in regenerative medicine. Among their many mechanisms of action, MSC extracellular vesicles (EVs) are a potential suitable substitute for MSCs in future cell-free-based therapeutic approaches. Unlike cells, EVs do not elicit acute immune rejection, and they can be produced in large quantities and stored until ready to use. Although the therapeutic potential of MSC EVs has already been proven, a thorough characterization of MSC EVs is lacking. In this work, we used a label-free liquid chromatography tandem mass spectrometry proteomic approach to identify the most abundant proteins in EVs that are secreted from MSCs derived from PSCs (PD-MSCs) and from their parental induced PSCs (iPSCs). Next, we compared both datasets and found that while iPSC EVs enclose proteins that modulate RNA and microRNA stability and protein sorting, PD-MSC EVs are rich in proteins that organize extracellular matrix, regulate locomotion, and influence cell–substrate adhesion. Moreover, compared to their respective cells, iPSCs and iPSC EVs share a greater proportion of proteins, while the PD-MSC proteome appears to be more specific. Correlation and principal component analysis consistently aggregate iPSCs and iPSC EVs but segregate PD-MSC and their EVs. Altogether, these findings suggest that during differentiation, compared with their parental iPSC EVs, PD-MSC EVs acquire a more specific set of proteins; arguably, this difference might confer their therapeutic properties.Fil: la Greca, Alejandro Damián. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Solari, Claudia María. Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Furmento, Verónica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lombardi, Antonella. Universidad de Buenos Aires; ArgentinaFil: Biani, María Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aban, Cyntia Estefania. Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica; ArgentinaFil: Moro, Lucía Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: García, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Guberman, Alejandra Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Sevlever, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Miriuka, Santiago Gabriel. Universidad Nacional de La Plata; ArgentinaFil: Luzzani, Carlos Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Minimal information for studies of extracellular vesicles 2018 (MISEV2018):a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points