Les dernières traces de Michel Beaulieu

Kaléidoscope et Trivialités se démarquent des précédents recueils de Michel Beaulieu par l’importance de l’érotisme comme exploration de soi. Isabelle Miron, dans cet article, s’intéresse au lien entre le temps et la souffrance dans l’oeuvre du poète, et propose d’observer, dans ce qu’elle désigne comme une « poétique du monologue intérieur » comment, dans les poèmes de Beaulieu, les rapports érotiques permettent de se soustraire à l’emprise du temps. À l’image de toute son oeuvre, cet échappatoire se caractérise par l’ambiguïté que l’on retrouve chez Schopenhauer : à la fois « affirmation et négation du vouloir-vivre ».Kaléidoscope and Trivialités differ from Michel Beaulieu’s previous collections of poems through the importance given to eroticism as self-exploration. The author, in this article, is interested in the connection between time and suffering in the poet’s work and proposes to observe, in what she describes as a “poetics of the interior monologue”, how, in Beaulieu’s poems, erotic relations provide a way of escaping from the grip of time. As in his work as a whole, this way out is characterized by the ambiguity expressed by Schopenhauer: both “assertion and negation of the will to live”.Kaléidoscope et Trivialités se desmarcan de los anteriores poemarios de Michel Beaulieu por la importancia del erotismo como exploración de uno mismo. En este artículo, la autora se interesa por el vínculo entre el tiempo y el sufrimiento en la obra del poeta y propone que se observe, en lo que ella designa como “una poética del monólogo interior”, cómo, en los poemas de Beaulieu, las relaciones eróticas permiten sustraerse a la influencia del tiempo. Al igual que toda su obra, esta escapatoria se caracteriza por la ambigüedad que encontramos en Schopenhauer: a la vez “afirmación y negación del deseo de vivir”

La quête de sens par le corps chez Michel Beaulieu et Juan Garcia

Thèse numérisée par la Direction des bibliothèques de l'Université de Montréal

Étude synchronique de la poésie au Québec et en France

Cet article explore la corrélation faite par les historiens de la littérature québécoise entre l'autonomisation de la littérature québécoise et la synchronie de son évolution avec la littérature française. De plus, lorsque la poésie des femmes du Québec et de la France est considérée du point de vue synchronique, une différence importante s'impose : l'impossibilité chez les poètes françaises d'accéder à la postérité quant à leur inscription dans l'histoire littéraire. Quelle est la nature de la capacité d'intégration au Québec qui permet non seulement que la poésie québécoise contemporaine incorpore de plus en plus des poètes migrants et d'autres qui, sans voyager, s'imprègnent de ce multiculturalisme qui peu à peu caractérise le Québec, mais aussi que les oeuvres de poètes comme Anne Hébert et Rina Lasnier soient retenues par l'histoire littéraire québécoise

Pierre Nepveu, Lectures des lieux

Dans ses Lectures des lieux, le poète et essayiste Pierre Nepveu nous convie à une vingtaine d’essais personnels, divisés en cinq parties, se situant dans le prolongement de son recueil d’essais précédent intitulé Intérieurs du Nouveau Monde (Boréal, 1998). Le premier essai, «Retour à Mirabel ou l’émotion du proche», porte sur sa vision d’un territoire immense sur lequel s’est construit, dans les années 1970 au Québec, l’Aéroport International de Mirabel, maintenant pratiquement déserté. Fais..

The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNA.

International audienceDNA damage checkpoints are signal transduction pathways that are activated after genotoxic insults to protect genomic integrity. At the site of DNA damage, 'mediator' proteins are in charge of recruiting 'signal transducers' to molecules 'sensing' the damage. Budding yeast Rad9, fission yeast Crb2 and metazoan 53BP1 are presented as mediators involved in the activation of checkpoint kinases. Here we show that, despite low sequence conservation, Rad9 exhibits a tandem tudor domain structurally close to those found in human/mouse 53BP1 and fission yeast Crb2. Moreover, this region is important for the resistance of Saccharomyces cerevisiae to different genotoxic stresses. It does not mediate direct binding to a histone H3 peptide dimethylated on K79, nor to a histone H4 peptide dimethylated on lysine 20, as was demonstrated for 53BP1. However, the tandem tudor region of Rad9 directly interacts with single-stranded DNA and double-stranded DNAs of various lengths and sequences through a positively charged region absent from 53BP1 and Crb2 but present in several yeast Rad9 homologs. Our results argue that the tandem tudor domains of Rad9, Crb2 and 53BP1 mediate chromatin binding next to double-strand breaks. However, their modes of chromatin recognition are different, suggesting that the corresponding interactions are differently regulated

Structural and functional characterization of the Mycobacterium tuberculosis uridine monophosphate kinase: insights into the allosteric regulation†

Nucleoside Monophosphate Kinases (NMPKs) family are key enzymes in nucleotide metabolism. Bacterial UMPKs depart from the main superfamily of NMPKs. Having no eukaryotic counterparts they represent attractive therapeutic targets. They are regulated by GTP and UTP, while showing different mechanisms in Gram(+), Gram(–) and archaeal bacteria. In this work, we have characterized the mycobacterial UMPK (UMPKmt) combining enzymatic and structural investigations with site-directed mutagenesis. UMPKmt exhibits cooperativity toward ATP and an allosteric regulation by GTP and UTP. The crystal structure of the complex of UMPKmt with GTP solved at 2.5 Å, was merely identical to the modelled apo-form, in agreement with SAXS experiments. Only a small stretch of residues was affected upon nucleotide binding, pointing out the role of macromolecular dynamics rather than major structural changes in the allosteric regulation of bacterial UMPKs. We further probe allosteric regulation by site-directed mutagenesis. In particular, a key residue involved in the allosteric regulation of this enzyme was identified

Determination of antioxidant compounds in foodstuff

© 2017 Scrivener Publishing LLC. Phenolic compounds, vitamins and carotenoids are naturally found in different foodstuff. These antioxidant compounds play an important role in human health and are of interest for the food, pharmaceutical and cosmeceutical industries. Modern chromatographic and spectrometric techniques have made analysis easier than ever before, but their success depends on the extraction method used. In fact, the different antioxidants are identifified using chromatographic techniques coupled to diff erent specififi c detectors according to the characteristics of each molecule. Beyond their well-known health-promoting effects, antioxidant molecules can also be used to functionalize or preserve the freshness, nutritive value, flflavor and color of foodstuff s, which justify their incorporation into several matrices. In this chapter, the most common antioxidant compounds in foodstuff will be described, as well as the methodologies involved in their extraction, separation, identifification and quantifification. The bioactive properties and industrial applications of these compounds through innovative techniques will also be taken into account.info:eu-repo/semantics/publishedVersio

Exploring the link between MORF4L1 and risk of breast cancer.

INTRODUCTION: Proteins encoded by Fanconi anemia (FA) and/or breast cancer (BrCa) susceptibility genes cooperate in a common DNA damage repair signaling pathway. To gain deeper insight into this pathway and its influence on cancer risk, we searched for novel components through protein physical interaction screens. METHODS: Protein physical interactions were screened using the yeast two-hybrid system. Co-affinity purifications and endogenous co-immunoprecipitation assays were performed to corroborate interactions. Biochemical and functional assays in human, mouse and Caenorhabditis elegans models were carried out to characterize pathway components. Thirteen FANCD2-monoubiquitinylation-positive FA cell lines excluded for genetic defects in the downstream pathway components and 300 familial BrCa patients negative for BRCA1/2 mutations were analyzed for genetic mutations. Common genetic variants were genotyped in 9,573 BRCA1/2 mutation carriers for associations with BrCa risk. RESULTS: A previously identified co-purifying protein with PALB2 was identified, MRG15 (MORF4L1 gene). Results in human, mouse and C. elegans models delineate molecular and functional relationships with BRCA2, PALB2, RAD51 and RPA1 that suggest a role for MRG15 in the repair of DNA double-strand breaks. Mrg15-deficient murine embryonic fibroblasts showed moderate sensitivity to γ-irradiation relative to controls and reduced formation of Rad51 nuclear foci. Examination of mutants of MRG15 and BRCA2 C. elegans orthologs revealed phenocopy by accumulation of RPA-1 (human RPA1) nuclear foci and aberrant chromosomal compactions in meiotic cells. However, no alterations or mutations were identified for MRG15/MORF4L1 in unclassified FA patients and BrCa familial cases. Finally, no significant associations between common MORF4L1 variants and BrCa risk for BRCA1 or BRCA2 mutation carriers were identified: rs7164529, Ptrend = 0.45 and 0.05, P2df = 0.51 and 0.14, respectively; and rs10519219, Ptrend = 0.92 and 0.72, P2df = 0.76 and 0.07, respectively. CONCLUSIONS: While the present study expands on the role of MRG15 in the control of genomic stability, weak associations cannot be ruled out for potential low-penetrance variants at MORF4L1 and BrCa risk among BRCA2 mutation carriers.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are

Identification of four novel susceptibility loci for oestrogen receptor negative breast cancer

Common variants in 94 loci have been associated with breast cancer including 15 loci with genome-wide significant associations (P<5 × 10−8) with oestrogen receptor (ER)-negative breast cancer and BRCA1-associated breast cancer risk. In this study, to identify new ER-negative susceptibility loci, we performed a meta-analysis of 11 genome-wide association studies (GWAS) consisting of 4,939 ER-negative cases and 14,352 controls, combined with 7,333 ER-negative cases and 42,468 controls and 15,252 BRCA1 mutation carriers genotyped on the iCOGS array. We identify four previously unidentified loci including two loci at 13q22 near KLF5, a 2p23.2 locus near WDR43 and a 2q33 locus near PPIL3 that display genome-wide significant associations with ER-negative breast cancer. In addition, 19 known breast cancer risk loci have genome-wide significant associations and 40 had moderate associations (P<0.05) with ER-negative disease. Using functional and eQTL studies we implicate TRMT61B and WDR43 at 2p23.2 and PPIL3 at 2q33 in ER-negative breast cancer aetiology. All ER-negative loci combined account for ∼11% of familial relative risk for ER-negative disease and may contribute to improved ER-negative and BRCA1 breast cancer risk prediction