Synchronization modulation increases transepithelial potentials in MDCK monolayers through Na/K pumps

Peer reviewedPublisher PD

Observation of associated near-side and away-side long-range correlations in √sNN=5.02 TeV proton-lead collisions with the ATLAS detector

Two-particle correlations in relative azimuthal angle (Δϕ) and pseudorapidity (Δη) are measured in √sNN=5.02  TeV p+Pb collisions using the ATLAS detector at the LHC. The measurements are performed using approximately 1  μb-1 of data as a function of transverse momentum (pT) and the transverse energy (ΣETPb) summed over 3.1<η<4.9 in the direction of the Pb beam. The correlation function, constructed from charged particles, exhibits a long-range (2<|Δη|<5) “near-side” (Δϕ∼0) correlation that grows rapidly with increasing ΣETPb. A long-range “away-side” (Δϕ∼π) correlation, obtained by subtracting the expected contributions from recoiling dijets and other sources estimated using events with small ΣETPb, is found to match the near-side correlation in magnitude, shape (in Δη and Δϕ) and ΣETPb dependence. The resultant Δϕ correlation is approximately symmetric about π/2, and is consistent with a dominant cos⁡2Δϕ modulation for all ΣETPb ranges and particle pT

Cr(VI) Removal from Aqueous Solution Using a Magnetite Snail Shell

© 2020, Springer Nature Switzerland AG. In this study, magnetic snail shell (MSS) prepared by impregnating of iron oxide onto snail shell (SS) powder was used for removing Cr(VI) from aqueous solution. Among six different mass ratios of Fe/SS powder studied, the MSS25 produced at a ratio of 25% achieved the highest Cr(VI) adsorption capacity. Batch adsorption experiments were conducted to investigate the adsorption isotherm, kinetics, and mechanism of Cr(VI) onto MSS25. The results illustrated that adsorption of Cr(VI) onto MSS25 reached equilibrium after 150 min at pH 3. The adsorption kinetics could be well described by the pseudo-second order model (R2 = 0.986). The Langmuir model (R2 = 0.971) was the best-fitting model that described the adsorption isotherm of Cr(VI) onto MSS25. The maximum adsorption capacity was 46.08 mg Cr(VI) per gram of MSS25. Ion exchange, electrostatic attraction, and adsorption-coupled reduction were determined as the main adsorption mechanisms of Cr(VI) onto MSS25. The high percentages of CaCO3 and Fe3O4 found in the MSS25 structure made a significant contribution to the Cr(VI) adsorption process

Phosphate Adsorption by Silver Nanoparticles-Loaded Activated Carbon derived from Tea Residue.

This study presents the removal of phosphate from aqueous solution using a new silver nanoparticles-loaded tea activated carbon (AgNPs-TAC) material. In order to reduce costs, the tea activated carbon was produced from tea residue. Batch adsorption experiments were conducted to evaluate the effects of impregnation ratio of AgNPs and TAC, pH solution, contact time, initial phosphate concentration and dose of AgNPs-AC on removing phosphate from aqueous solution. Results show that the best conditions for phosphate adsorption occurred at the impregnation ratio AgNPs/TAC of 3% w/w, pH 3, and contact time lasting 150 min. The maximum adsorption capacity of phosphate on AgNPs-TAC determined by the Langmuir model was 13.62 mg/g at an initial phosphate concentration of 30 mg/L. The adsorption isotherm of phosphate on AgNPs-TAC fits well with both the Langmuir and Sips models. The adsorption kinetics data were also described well by the pseudo-first-order and pseudo-second-order models with high correlation coefficients of 0.978 and 0.966, respectively. The adsorption process was controlled by chemisorption through complexes and ligand exchange mechanisms. This study suggests that AgNPs-TAC is a promising, low cost adsorbent for phosphate removal from aqueous solution

Addendum to ‘measurement of the tt̄ production cross-section using eμ events with b-tagged jets in pp collisions at √s= 7 and 8 TeV with the ATLAS detector’

The ATLAS measurement of the inclusive top quark pair (tt̄) cross-section σtt̄ in proton–proton collisions at √s=8 TeV has been updated using the final 2012 luminosity calibration. The updated cross-section result is: σtt¯=242.9±1.7±5.5±5.1±4.2pb, where the four uncertainties arise from data statistics, experimental and theoretical systematic effects, knowledge of the integrated luminosity and of the LHC beam energy. The result is consistent with theoretical QCD calculations at next-to-next-to-leading order. The measurement of the ratio of tt̄ cross-sections at √s=8 TeV and √s=7 TeV, and the √s=8 TeV fiducial measurement corresponding to the experimental acceptance of the leptons, have also been updated. The most precise measurement of the tt̄ cross-section (σtt̄) in proton–proton collisions at √s=8 TeV from the ATLAS Collaboration was made using events with an opposite-charge electron–muon pair and one or two b-tagged jets [1], and used a preliminary calibration of the integrated luminosity. The luminosity calibration has been finalised since [2] with a total uncertainty of 1.9%, corresponding to a substantial improvement on the previous uncertainty of 2.8%. Since the uncertainty on the integrated luminosity contributed 3.1% of the total 4.3% uncertainty on the σtt¯ measurement reported in [1], a significant improvement in the measurement is possible by using the new luminosity calibration, as documented in this Addendum. The new calibration corresponds to an integrated luminosity of 20.2 fb−¹ for the √s=8 TeV sample, a decrease of 0.2%. The cross-section was recomputed taking into account the effects on both the conversion of the tt¯ event yield to a cross-section, and the background estimates, giving a result of: σtt¯=242.9±1.7±5.5±5.1±4.2pb, where the four uncertainties arise from data statistics, experimental and theoretical systematic effects, knowledge of the integrated luminosity, and of the LHC beam energy, giving a total uncertainty of 8.8 pb (3.6 %). The result is consistent with the theoretical prediction of 252.9−14.5+13.3 pb, calculated at next-to-next-to-leading-order with next-to-next-to-leading-logarithmic soft gluon terms with the top++ 2.0 program [3] as discussed in detail in Ref. [1]. The updated value of the ratio of cross-sections Rtt¯=σtt¯(8 TeV)/σtt¯(7 TeV) is: Rtt¯=1.328±0.024±0.015±0.038±0.001, with uncertainties defined as above, adding in quadrature to a total of 0.047. The largest uncertainty comes from the uncertainties on the integrated luminosities, considered to be uncorrelated between the √s=7 TeV and √s=8 TeV datasets. This result is 2.1σ below the expectation of 1.430±0.013 calculated from top++ 2.0 as discussed in Ref. [1]. The updated fiducial cross-sections, for a tt¯ decay producing an eμ pair within a given fiducial region, are shown in Table 1, updating Table 5 of Ref. [1]. The results are given both for the analysis requirements of pT>25GeV and |η|30GeV and |η|<2.4. They are given separately for the two cases where events with either one or both leptons coming from t→W→τ→ℓ rather than the direct decay t→W→ℓ(ℓ=e or μ) are included, or where the contributions involving τ decays are subtracted. The results shown for the √s=7 TeV data sample are unchanged with respect to those in Ref. [1]. The results for the top quark pole mass and limits on light supersymmetric top squarks presented in Ref. [1] are derived from √s=7 TeV and √s=8 TeV cross-section measurements taken together, and would be only slightly improved by the luminosity update described here

Flavor tagged time-dependent angular analysis of the B0s → J/ψϕ decay and extraction of ΔΓs and the weak phase ϕs in ATLAS

A measurement of the B0s→J/ψϕ decay parameters, updated to include flavor tagging is reported using 4.9  fb−¹ of integrated luminosity collected by the ATLAS detector from √s=7  TeV pp collisions recorded in 2011 at the LHC. The values measured for the physical parameters are ϕs=0.12±0.25(stat)±0.05(syst)  rad ΔΓs=0.053±0.021(stat)±0.010(syst)  ps−¹ Γs=0.677±0.007(stat)±0.004(syst)  ps−¹ |A∥(0)|2=0.220±0.008(stat)±0.009(syst) |A0(0)|2=0.529±0.006(stat)±0.012(syst) δ⊥=3.89±0.47(stat)±0.11(syst)  rad where the parameter ΔΓs is constrained to be positive. The S-wave contribution was measured and found to be compatible with zero. Results for ϕs and ΔΓs are also presented as 68% and 95% likelihood contours, which show agreement with the Standard Model expectations

A novel approach to glaucoma screening and education in Nepal

<p>Abstract</p> <p>Background</p> <p>Glaucoma is a major cause of blindness worldwide and an increasingly significant global health problem. Glaucoma prevention and management efforts have been challenging due to inherent difficulty in developing a simple and cost-effective screening plan, limited access to health care and educational resources, poverty, and inadequate knowledge of the disease, particularly in developing countries. Starting in 2004 the Tilganga Eye Centre in Kathmandu, Nepal has provided targeted glaucoma screening, treatment, and education through a combination of clinical outreach programs and educational activities for patients.</p> <p>Methods</p> <p>A simple, age-based glaucoma screening algorithm was incorporated into three one-day cataract screening clinics. Using this algorithm, patients who were newly diagnosed with glaucoma were referred to TEC, where medication and surgery were provided free of charge through private donor funding. In addition, we describe two ongoing educational programs for increasing glaucoma awareness: an annual Glaucoma Awareness Week (which includes free screening, treatment, and counseling), and a repeating lecture series which generates new counselors.</p> <p>Results</p> <p>From 2004 to 2007 screening at the annual Glaucoma Awareness Week resulted in the diagnosis of 120 individuals with glaucoma, or 7.6% of total registrants. Attendance increased annually with a trend toward an increasing number of returning patients but a decreasing percentage of newly diagnosed patients, though the absolute numbers have remained relatively stable (range 21 to 38). Data from the three one-day screening clinics in 2006 show that approximately 2 to 4% of patients 50 years of age or older per clinic were newly diagnosed with POAG.</p> <p>Conclusion</p> <p>This multi-faceted approach appears to successfully identify individuals with glaucoma and provide treatment to those who would otherwise not be able to afford it. While more data is needed to validate this model, specifically regarding the effectiveness of educational activities, long-term visual outcomes, and medication compliance, it may serve as a useful framework for other developing countries with similarly limited resources.</p

Small-molecule inhibition of METTL3 as a strategy against myeloid leukaemia.

N6-methyladenosine (m6A) is an abundant internal RNA modification1,2 that is catalysed predominantly by the METTL3-METTL14 methyltransferase complex3,4. The m6A methyltransferase METTL3 has been linked to the initiation and maintenance of acute myeloid leukaemia (AML), but the potential of therapeutic applications targeting this enzyme remains unknown5-7. Here we present the identification and characterization of STM2457, a highly potent and selective first-in-class catalytic inhibitor of METTL3, and a crystal structure of STM2457 in complex with METTL3-METTL14. Treatment of tumours with STM2457 leads to reduced AML growth and an increase in differentiation and apoptosis. These cellular effects are accompanied by selective reduction of m6A levels on known leukaemogenic mRNAs and a decrease in their expression consistent with a translational defect. We demonstrate that pharmacological inhibition of METTL3 in vivo leads to impaired engraftment and prolonged survival in various mouse models of AML, specifically targeting key stem cell subpopulations of AML. Collectively, these results reveal the inhibition of METTL3 as a potential therapeutic strategy against AML, and provide proof of concept that the targeting of RNA-modifying enzymes represents a promising avenue for anticancer therapy

Keys and seats: Spatial response coding underlying the joint spatial compatibility effect

Spatial compatibility effects (SCEs) are typically observed when participants have to execute spatially defined responses to nonspatial stimulus features (e.g., the color red or green) that randomly appear to the left and the right. Whereas a spatial correspondence of stimulus and response features facilitates response execution, a noncorrespondence impairs task performance. Interestingly, the SCE is drastically reduced when a single participant responds to one stimulus feature (e.g., green) by operating only one response key (individual go/no-go task), whereas a full-blown SCE is observed when the task is distributed between two participants (joint go/no-go task). This joint SCE (a.k.a. the social Simon effect) has previously been explained by action/task co-representation, whereas alternative accounts ascribe joint SCEs to spatial components inherent in joint go/no-go tasks that allow participants to code their responses spatially. Although increasing evidence supports the idea that spatial rather than social aspects are responsible for joint SCEs emerging, it is still unclear to which component(s) the spatial coding refers to: the spatial orientation of response keys, the spatial orientation of responding agents, or both. By varying the spatial orientation of the responding agents (Exp. 1) and of the response keys (Exp. 2), independent of the spatial orientation of the stimuli, in the present study we found joint SCEs only when both the seating and the response key alignment matched the stimulus alignment. These results provide evidence that spatial response coding refers not only to the response key arrangement, but also to the—often neglected—spatial orientation of the responding agents

Llama Antibody Fragments Recognizing Various Epitopes of the CD4bs Neutralize a Broad Range of HIV-1 Subtypes A, B and C

Many of the neutralising antibodies, isolated to date, display limited activities against the globally most prevalent HIV-1 subtypes A and C. Therefore, those subtypes are considered to be an important target for antibody-based therapy. Variable domains of llama heavy chain antibodies (VHH) have some superior properties compared with classical antibodies. Therefore we describe the application of trimeric forms of envelope proteins (Env), derived from HIV-1 of subtype A and B/C, for a prolonged immunization of two llamas. A panel of VHH, which interfere with CD4 binding to HIV-1 Env were selected with use of panning. The results of binding and competition assays to various Env, including a variant with a stabilized CD4-binding state (gp120Ds2), cross-competition experiments, maturation analysis and neutralisation assays, enabled us to classify the selected VHH into three groups. The VHH of group I were efficient mainly against viruses of subtype A, C and B′/C. The VHH of group II resemble the broadly neutralising antibody (bnmAb) b12, neutralizing mainly subtype B and C viruses, however some had a broader neutralisation profile. A representative of the third group, 2E7, had an even higher neutralization breadth, neutralizing 21 out of the 26 tested strains belonging to the A, A/G, B, B/C and C subtypes. To evaluate the contribution of certain amino acids to the potency of the VHH a small set of the mutants were constructed. Surprisingly this yielded one mutant with slightly improved neutralisation potency against 92UG37.A9 (subtype A) and 96ZM651.02 (subtype C). These findings and the well-known stability of VHH indicate the potential application of these VHH as anti-HIV-1 microbicides