A Huttenlocher

AC Vieira

AV Ljubimov

B Reid

B Robertson

B Song

B Vanhaesebroeck

Benjamin Lee

CD McCaig

CJ Taylor

CM Festen

D Yu

DM Bryant

DM Maurice

E Mayhew

EH Larsen

EH Serpersu

Hanqing Li

Hong-Long (James) Ji

J Dube

J Teissie

J Zak

JG Hayes

JP Lavelle

KM Madsen

L Shoshani

L Zhang

Lin Cao

LP Zhang

M Blank

M Chiang

M Gekle

M Nakao

M Zhao

MD Laughery

Michelle So

Min Zhao

MR Knowles

N Montalbetti

OA Candia

PD Wilson

PG Lawrence

R Dando

R Kucerova

R Nuccitelli

RC Valente

RF Rakowski

SA Rajasekaran

SD Klyce

TC Chinet

TD Xie

VS Markin

Vu Tran

W Chen

Wei Chen

WS Marshall

X Leschelle

Xiaodong Zhang

Yaohui Sun

PLoS ONE

English

PubMed

Transepithelial potential (TEP) is the voltage across a polarized epithelium. In epithelia that have active transport functions, the force for transmembrane flux of an ion is dictated by the electrochemical gradient in which TEP plays an essential role. In epithelial injury, disruption of the epithelial barrier collapses the TEP at the wound edge, resulting in the establishment of an endogenous wound electric field (∼100 mV/mm) that is directed towards the center of the wound. This endogenous electric field is implicated to enhance wound healing by guiding cell migration. We thus seek techniques to enhance the TEP, which may increase the wound electric fields and enhance wound healing. We report a novel technique, termed synchronization modulation (SM) using a train of electric pulses to synchronize the Na/K pump activity, and then modulating the pumping cycles to increase the efficiency of the Na/K pumps. Kidney epithelial monolayers (MDCK cells) maintain a stable TEP and transepithelial resistance (TER). SM significantly increased TEP over four fold. Either ouabain or digoxin, which block Na/K pump, abolished SM-induced TEP increases. In addition to the pump activity, basolateral distribution of Na/K pumps is essential for an increase in TEP. Our study for the first time developed an electrical approach to significantly increase the TEP. This technique targeting the Na/K pump may be used to modulate TEP, and may have implication in wound healing and in diseases where TEP needs to be modulated

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Synchronization modulation increases transepithelial potentials in MDCK monolayers through Na/K pumps.

Crossref

Synchronization Modulation Increases Transepithelial Potentials in MDCK Monolayers through Na/K Pumps

Tran, Vu

Zhang, Xiaodong

Cao, Lin

Li, Hanqing

Lee, Benjamin

So, Michelle

Sun, Yaohui

Chen, Wei

Zhao, Min

eScholarship - University of California

Aberdeen University Research

Synchronization modulation increases transepithelial potentials in MDCK monolayers through Na/K pumps

http://dx.plos.org/10.1371/journal.pone.0061509

Synchronization modulation increases transepithelial potentials in MDCK monolayers through Na/K pumps

Abstract

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Directory of Open Access Journals

Crossref

eScholarship - University of California

Crossref

Aberdeen University Research