The "BIT TAX" : the case for further research

Abstract not availableeconomics of technology ;

The kinematics and excitation of infrared water vapor emission from planet-forming disks: results from spectrally-resolved surveys and guidelines for JWST spectra

This work presents water emission spectra at wavelengths covered by JWST (2.9-12.8 $\mu$m) as spectrally-resolved with high resolving powers (R = 30,000-100,000) using ground-based spectrographs. Two new surveys with iSHELL and VISIR are combined with previous spectra from CRIRES and TEXES to cover parts of multiple ro-vibrational and rotational bands observable within telluric transmission bands, for a total of 85 disks and $\approx160$ spectra. The general expectation of a range of regions and excitation conditions traced by infrared water spectra is for the first time supported by the combined kinematics and excitation as spectrally resolved at multiple wavelengths. The main findings from this analysis are: 1) water lines are progressively narrower going from the ro-vibrational bands at 2-9 $\mu$m to the rotational lines at 12 $\mu$m, and partly match a broad (BC) and narrow (NC) emission components, respectively, as extracted from ro-vibrational CO spectra; 2) rotation diagrams of resolved water lines from upper level energies of 4000-9500 K show curvatures indicative of optically thick emission ($\approx 10^{18}$ cm$^{-2}$) from a range of excitation temperatures ($\approx$ 800-1100 K); 3) the new 5 $\mu$m spectra demonstrate that slab model fits to the rotational lines at $> 10 \mu$m strongly over-predict the ro-vibrational emission bands at $< 9 \mu$m, implying non-LTE excitation. We discuss these findings in the context of a emission from a disk surface and a molecular inner disk wind, and provide a list of detailed guidelines to support the analysis and interpretation of spectrally-unresolved JWST spectra.Comment: Posted on arXiv as submitted to AJ, for immediate access by teams working on the analysis of JWST spectr

Resolving the CO Snow Line in the Disk around HD 163296

We report Submillimeter Array (SMA) observations of CO (J=2--1, 3--2 and 6--5) and its isotopologues (13CO J=2--1, C18O J=2--1 and C17O J=3--2) in the disk around the Herbig Ae star HD 163296 at ~2" (250 AU) resolution, and interpret these data in the framework of a model that constrains the radial and vertical location of the line emission regions. First, we develop a physically self-consistent accretion disk model with an exponentially tapered edge that matches the spectral energy distribution and spatially resolved millimeter dust continuum emission. Then, we refine the vertical structure of the model using wide range of excitation conditions sampled by the CO lines, in particular the rarely observed J=6--5 transition. By fitting 13CO data in this structure, we further constrain the vertical distribution of CO to lie between a lower boundary below which CO freezes out onto dust grains (T ~ 19 K) and an upper boundary above which CO can be photodissociated (the hydrogen column density from the disk surface is ~ 10^{21} cm-2). The freeze-out at 19 K leads to a significant drop in the gas-phase CO column density beyond a radius of ~155 AU, a "CO snow line" that we directly resolve. By fitting the abundances of all CO isotopologues, we derive isotopic ratios of 12C/13C, 16O/18O and 18O/17O that are consistent with quiescent interstellar gas-phase values. This detailed model of the HD 163296 disk demonstrates the potential of a staged, parametric technique for constructing unified gas and dust structure models and constraining the distribution of molecular abundances using resolved multi-transition, multi-isotope observations.Comment: 40 pages, 13 figures, accepted for publication in Ap

The TW Hya Disk at 870 microns: Comparison of CO and Dust Radial Structures

We present high resolution (0.3" = 16 AU), high signal-to-noise ratio Submillimeter Array observations of the 870 microns (345 GHz) continuum and CO J=3--2 line emission from the protoplanetary disk around TW Hya. Using continuum and line radiative transfer calculations, those data and the multiwavelength spectral energy distribution are analyzed together in the context of simple two-dimensional parametric disk structure models. Under the assumptions of a radially invariant dust population and (vertically integrated) gas-to-dust mass ratio, we are unable to simultaneously reproduce the CO and dust observations with model structures that employ either a single, distinct outer boundary or a smooth (exponential) taper at large radii. Instead, we find that the distribution of millimeter-sized dust grains in the TW Hya disk has a relatively sharp edge near 60 AU, contrary to the CO emission (and optical/infrared scattered light) that extends to a much larger radius of at least 215 AU. We discuss some possible explanations for the observed radial distribution of millimeter-sized dust grains and the apparent CO-dust size discrepancy, and suggest that they may be hallmarks of substructure in the dust disk or natural signatures of the growth and radial drift of solids that might be expected for disks around older pre-main sequence stars like TW Hya.Comment: ApJ, in press (fixed typo in Equation 4

Targeting of human interleukin-12B by small hairpin RNAs in xenografted psoriatic skin

<p>Abstract</p> <p>Background</p> <p>Psoriasis is a chronic inflammatory skin disorder that shows as erythematous and scaly lesions. The pathogenesis of psoriasis is driven by a dysregulation of the immune system which leads to an altered cytokine production. Proinflammatory cytokines that are up-regulated in psoriasis include tumor necrosis factor alpha (TNFα), interleukin-12 (IL-12), and IL-23 for which monoclonal antibodies have already been approved for clinical use. We have previously documented the therapeutic applicability of targeting TNFα mRNA for RNA interference-mediated down-regulation by anti-TNFα small hairpin RNAs (shRNAs) delivered by lentiviral vectors to xenografted psoriatic skin. The present report aims at targeting mRNA encoding the shared p40 subunit (IL-12B) of IL-12 and IL-23 by cellular transduction with lentiviral vectors encoding anti-IL12B shRNAs.</p> <p>Methods</p> <p>Effective anti-IL12B shRNAs are identified among a panel of shRNAs by potency measurements in cultured cells. The efficiency and persistency of lentiviral gene delivery to xenografted human skin are investigated by bioluminescence analysis of skin treated with lentiviral vectors encoding the luciferase gene. shRNA-expressing lentiviral vectors are intradermally injected in xenografted psoriatic skin and the effects of the treatment evaluated by clinical psoriasis scoring, by measurements of epidermal thickness, and IL-12B mRNA levels.</p> <p>Results</p> <p>Potent and persistent transgene expression following a single intradermal injection of lentiviral vectors in xenografted human skin is reported. Stable IL-12B mRNA knockdown and reduced epidermal thickness are achieved three weeks after treatment of xenografted psoriatic skin with lentivirus-encoded anti-IL12B shRNAs. These findings mimick the results obtained with anti-TNFα shRNAs but, in contrast to anti-TNFα treatment, anti-IL12B shRNAs do not ameliorate the psoriatic phenotype as evaluated by semi-quantitative clinical scoring and by immunohistological examination.</p> <p>Conclusions</p> <p>Our studies consolidate the properties of lentiviral vectors as a tool for potent gene delivery and for evaluation of mRNA targets for anti-inflammatory therapy. However, in contrast to local anti-TNFα treatment, the therapeutic potential of targeting IL-12B at the RNA level in psoriasis is questioned.</p

The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP): illuminating the functional diversity of eukaryotic life in the oceans through transcriptome sequencing

International audienceCurrent sampling of genomic sequence data from eukaryotes is relatively poor, biased, and inadequate to address important questions about their biology, evolution, and ecology; this Community Page describes a resource of 700 transcriptomes from marine microbial eukaryotes to help understand their role in the world's oceans

Causes of genome instability: the effect of low dose chemical exposures in modern society.

Genome instability is a prerequisite for the development of cancer. It occurs when genome maintenance systems fail to safeguard the genome's integrity, whether as a consequence of inherited defects or induced via exposure to environmental agents (chemicals, biological agents and radiation). Thus, genome instability can be defined as an enhanced tendency for the genome to acquire mutations; ranging from changes to the nucleotide sequence to chromosomal gain, rearrangements or loss. This review raises the hypothesis that in addition to known human carcinogens, exposure to low dose of other chemicals present in our modern society could contribute to carcinogenesis by indirectly affecting genome stability. The selected chemicals with their mechanisms of action proposed to indirectly contribute to genome instability are: heavy metals (DNA repair, epigenetic modification, DNA damage signaling, telomere length), acrylamide (DNA repair, chromosome segregation), bisphenol A (epigenetic modification, DNA damage signaling, mitochondrial function, chromosome segregation), benomyl (chromosome segregation), quinones (epigenetic modification) and nano-sized particles (epigenetic pathways, mitochondrial function, chromosome segregation, telomere length). The purpose of this review is to describe the crucial aspects of genome instability, to outline the ways in which environmental chemicals can affect this cancer hallmark and to identify candidate chemicals for further study. The overall aim is to make scientists aware of the increasing need to unravel the underlying mechanisms via which chemicals at low doses can induce genome instability and thus promote carcinogenesis

Photography-based taxonomy is inadequate, unnecessary, and potentially harmful for biological sciences

The question whether taxonomic descriptions naming new animal species without type specimen(s) deposited in collections should be accepted for publication by scientific journals and allowed by the Code has already been discussed in Zootaxa (Dubois & Nemésio 2007; Donegan 2008, 2009; Nemésio 2009a–b; Dubois 2009; Gentile & Snell 2009; Minelli 2009; Cianferoni & Bartolozzi 2016; Amorim et al. 2016). This question was again raised in a letter supported by 35 signatories published in the journal Nature (Pape et al. 2016) on 15 September 2016. On 25 September 2016, the following rebuttal (strictly limited to 300 words as per the editorial rules of Nature) was submitted to Nature, which on 18 October 2016 refused to publish it. As we think this problem is a very important one for zoological taxonomy, this text is published here exactly as submitted to Nature, followed by the list of the 493 taxonomists and collection-based researchers who signed it in the short time span from 20 September to 6 October 2016