Polymerase Chain Reaction Screening for Fungemia and/or Invasive Fungal Infections in Patients with Hematologic Malignancies

INTRODUCTION: Invasive fungal infections (IFIs) are a life-threatening complication in patients with hematologic malignancies, mainly in acute leukemia patients, following chemotherapy. IFI incidence is increasing, and associated mortality remains high due to unreliable diagnosis. Antifungal drugs are often limited by inadequate antimicrobial spectrum and side effects. Thus, the detection of circulating fungal DNA has been advocated as a rapid, more sensitive diagnostic tool. PATIENTS AND METHODS: Between June 01 and January 03, weekly blood samples (1,311) were screened from 193 patients undergoing intensive myelosuppressive or immunosuppressive therapy. IFI cases were classified according to European Organization for Research and Treatment of Cancer/Mycoses Study Group criteria. Fungal DNA was extracted from whole blood and amplified using polymerase chain reaction (PCR) published primers that bind to the conserved regions of the fungal 18S rRNA gene sequence. In our study, two or more consecutive positive samples were always associated with fungal disease. RESULTS: PCR screening predicted the development of IFI to be 17 days (median). This test had a specificity of 91.1% and a sensitivity of 75%. IFI incidence was 7.8%. DISCUSSION: Therefore, our results confirm the potential usefulness of PCR serial screening and the clinical applicability in everyday routine. PCR screening offers a noninvasive repeatable aid to the diagnosis of IFI

Two rapid assays for screening of patulin biodegradation

Artículo sobre distintos ensayos para comprobar la biodegradación de la patulinaThe mycotoxin patulin is produced by the blue mould pathogen Penicillium expansum in rotting apples during postharvest storage. Patulin is toxic to a wide range of organisms, including humans, animals, fungi and bacteria. Wash water from apple packing and processing houses often harbours patulin and fungal spores, which can contaminate the environment. Ubiquitous epiphytic yeasts, such as Rhodosporidium kratochvilovae strain LS11 which is a biocontrol agent of P. expansum in apples, have the capacity to resist the toxicity of patulin and to biodegrade it. Two non-toxic products are formed. One is desoxypatulinic acid. The aim of the work was to develop rapid, high-throughput bioassays for monitoring patulin degradation in multiple samples. Escherichia coli was highly sensitive to patulin, but insensitive to desoxypatulinic acid. This was utilized to develop a detection test for patulin, replacing time-consuming thin layer chromatography or high-performance liquid chromatography. Two assays for patulin degradation were developed, one in liquid medium and the other in semi-solid medium. Both assays allow the contemporary screening of a large number of samples. The liquid medium assay utilizes 96-well microtiter plates and was optimized for using a minimum of patulin. The semisolid medium assay has the added advantage of slowing down the biodegradation, which allows the study and isolation of transient degradation products. The two assays are complementary and have several areas of utilization, from screening a bank of microorganisms for biodegradation ability to the study of biodegradation pathways

Detection of dark galaxies and circum-galactic filaments fluorescently illuminated by a quasar at z=2.4

A deep narrow-band survey for Ly-alpha emission carried out on the VLT-FORS2 has revealed 98 Ly-alpha candidates down to a flux limit of 4.e-18 erg/s/cm^2 in a volume of 5500 comoving Mpc^3 at z=2.4 centered on the hyperluminous quasar HE0109-3518. The properties of the detected sources in terms of their i) equivalent width distribution, ii) luminosity function, and iii) the average luminosity versus projected distance from the quasar, all suggest that a large fraction of these objects have been fluorescently "illuminated" by HE0109-3518. This conclusion is supported by comparison with detailed radiative transfer simulations of the effects of the quasar illumination. 18 objects have a rest-frame Equivalent Width (EW0) larger than 240A, the expected limit for Ly-alpha emission powered by Population II star formation and 12 sources among these do not have any continuum counterpart in a deep V-band imaging of the same field. For these, a stacking analysis indicates EW0>800A, effectively ruling out Ly-alpha powered by internal star formation. These sources are thus the best candidates so far for proto-galactic clouds or "dark" galaxies at high-redshift, whose existence has recently been suggested by several theoretical studies. Assuming they are mostly ionized by the quasar radiation, we estimate that their gas masses would be about 10^9 Msun implying that their star formation efficiencies (SFE) are less than 1.e-11 yr^-1 several times below the SFE of the most gas-rich dwarf galaxies locally, and five hundred times lower than typical massive star-forming galaxies at z~2. We have also discovered extended, filamentary gas, also likely illuminated by the quasar, around some of the brightest continuum-detected sources with EW0>240A. This emission is compatible with the expectations for circum-galactic cold streams but other origins, including tidal stripping, are also possible.Comment: 26 pages, 16 figures; MNRAS in press (accepted 2012 Jun 15); minor changes from previous version, typos corrected, references adde

Clues to the nature of damped Lyman alpha systems from chemical evolution models

The evolution of the metallicity of damped Lyman alpha systems (DLAs) is investigated in order to understand the nature of these systems. The observational data on chemical abundances of DLAs are analysed with robust statistical methods, and the abundances are corrected for dust depletion. The results of this analysis are compared to predictions of several classes of chemical evolution models: one-zone dwarf galaxy models, multizone disk models, and chemodynamical models representing dwarf galaxies. We compare the observational data on the [alpha/Fe] and [N/alpha] ratios to the predictions from the models. In DLAs, these ratios are only partially reproduced by the dwarf galaxy one-zone model and by the disk model. On the other hand, the chemodynamical model for dwarf galaxies reproduces the properties of nearly all DLAs. We derive the formation epoch of dwarf galaxies, and we find that dwarf galaxies make a significant contribution to the total neutral gas density in DLAs, and that this contribution is more important at high redshifts (z > 2-3). We propose a scenario in which the DLA population is dominated by dwarf galaxies at high redshifts and by disks at lower redshifts. We also find that Lyman Break Galaxies (LBGs) may constitute a sequence rather than present a sharp dichotomy between the two populations. We also arise the possibility that we could be missing a whole population of high HI density column objects, with metallicities intermediate between those of DLAs and LBGs. Finally, we discuss the possibility that relying only on the observations of DLAs could lead to an underestimate of the metal content of the high redshift Universe.Comment: 23 pages, 19 figures. Accepted for publication in MNRA

Effects of bromopride on the healing of left colon anastomoses of rats

Objetivo: Avaliar os efeitos da bromoprida sobre a formação de aderências e a cicatrização de anastomoses de cólon esquerdo de ratos. Métodos: Foram incluídos 40 ratos, divididos em dois grupos contendo 20 animais, para administração de bromoprida (grupo de estudo- E) ou solução fisiológica (grupo controle- C). Cada grupo foi dividido em subgrupos contendo 10 animais cada, para eutanásia no terceiro (E3 e C3) ou no sétimo dia (E7 e C7) de pós-operatório. Os ratos foram submetidos à secção do cólon esquerdo e anastomose término-terminal. No dia da relaparotomia, foi avaliada a quantidade total de aderências e removido um segmento colônico contendo a anastomose para análise histopatológica, da força de ruptura e da concentração de hidroxiprolina. Resultados: Não houve diferença entre os grupos em relação à evolução clínica. Dois animais do grupo de estudo apresentaram deiscência de anastomose bloqueada. Os animais que receberam bromoprida apresentaram número de aderências intracavitárias e aderências à anastomose semelhantes ao grupo controle. As anastomoses dos animais do grupo E3 apresentaram menor resistência de ruptura do que as do grupo C3 (p=0,04). Este efeito não ocorreu no sétimo dia de pós-operatório (p=0,37). Não houve diferença significativa entre os grupos em relação à histopatologia ou concentração de hidroxiprolina das anastomoses. Conclusão: O uso da bromoprida está associado à diminuição da resistência tênsil de anastomoses do cólon esquerdo de ratos no terceiro dia de pós-operatório.Objective: To evaluate the effects of bromopride on the formation of adhesions and anastomotic healing in the left colon of rats. Methods: We divided 40 rats into two groups of 20 animals, administration of bromopride (study group-E) or saline (control group- C). Each group was divided into subgroups containing 10 animals each for euthanasia in the third (C3 and E3) or the seventh (E7 and C7) postoperative days. The rats were submitted to section of the left colon and end-to-end anastomosis. On the day of reoperation, we evaluated the total amount of adhesions and removed a colonic segment containing the anastomosis for histopathological analysis, assessment of rupture strength and hydroxyproline concentration. Results: There was no difference between groups in relation to clinical outcome. Two animals in the study group had blocked anastomotic leakage. The animals that received bromopride had the number of intracavitary adhesions and adhesions to the anastomosis similar to the control group. The anastomoses from the group E3 animals showed lower resistance to rupture the one from the C3 group (p = 0.04). This effect did not occur on the seventh postoperative day (p = 0.37). There was no significant difference between groups in relation to histopathology and hydroxyproline concentration in the anastomoses. Conclusion: The use of bromopride was associated with decreased tensile strength of left colon anastomosis in rats in the third postoperative day

Galaxy Formation Theory

We review the current theory of how galaxies form within the cosmological framework provided by the cold dark matter paradigm for structure formation. Beginning with the pre-galactic evolution of baryonic material we describe the analytical and numerical understanding of how baryons condense into galaxies, what determines the structure of those galaxies and how internal and external processes (including star formation, merging, active galactic nuclei etc.) determine their gross properties and evolution. Throughout, we highlight successes and failings of current galaxy formation theory. We include a review of computational implementations of galaxy formation theory and assess their ability to provide reliable modelling of this complex phenomenon. We finish with a discussion of several "hot topics" in contemporary galaxy formation theory and assess future directions for this field.Comment: 58 pages, to appear in Physics Reports. This version includes minor corrections and a handful of additional reference