Animal Tracks Northern Forest Action Pack

Welcome to Animal Tracks®, a classroom education program of the National Wildlife Federation focusing on teacher training and environmental education resources. In Animal Tracks materials, the animals and their tracks lead educators and students on an exploration of conservation issues. Thank you for using this Action Pack, our newest resource. We hope you find the Action Packs useful and as a \u27\u27work-in-progress,\u27\u27 we welcome any comments you might have for improvements. As you turn the page you\u27ll see our questionnaire. Please take a minute to fill it out and put it in the mail. We\u27ll include you on our mailing list and you\u27ll get invitations to any Animal Tracks educator workshops that we hold in your area as well as the latest information on Animal Tracks programs and materials. Animal Tracks Workshops are a large part of the Animal Tracks program. The Action Pack series was originally developed as our teacher training module. The workshops are designed to help teachers easily fit environmental and conservation issues into their lesson plans across the curriculum. Animal Tracks workshops emphasize learning by doing and include an interactive discussion of how to successfully incorporate action projects into learning. Animal Tracks has educator materials and information available online at http://www.nwf.orglatracks including the Water and Habitat Action Packs, Current Events Hotline, information about NWF\u27s EarthTomorrow program for Detroit area schools, Environmental Education Online Conference, Animal Tracks Workshop schedule, and Animal Tracks Online classroom activities. There are also Animal Tracks kids\u27 pages at http://www.nwf.orglnwflkids/ with our Cool Tour of the Environment, Ranger Rick site, resources in Spanish, games, and more fun. The next pages of the Action Pack are a questionnaire and an explanation about how to effectively use the Animal Tracks Action Packs. Again, we hope you find this a valuable resource and be sure to check out all the Animal Tracks Action Pack titles

Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule

Circulating tumor cells consist of phenotypically distinct subpopulations that originate from the tumor microenvironment. We report a circulating tumor cell dual selection assay that uses discrete microfluidics to select circulating tumor cell subpopulations from a single blood sample; circulating tumor cells expressing the established marker epithelial cell adhesion molecule and a new marker, fibroblast activation protein alpha, were evaluated. Both circulating tumor cell subpopulations were detected in metastatic ovarian, colorectal, prostate, breast, and pancreatic cancer patients and 90% of the isolated circulating tumor cells did not co-express both antigens. Clinical sensitivities of 100% showed substantial improvement compared to epithelial cell adhesion molecule selection alone. Owing to high purity (>80%) of the selected circulating tumor cells, molecular analysis of both circulating tumor cell subpopulations was carried out in bulk, including next generation sequencing, mutation analysis, and gene expression. Results suggested fibroblast activation protein alpha and epithelial cell adhesion molecule circulating tumor cells are distinct subpopulations and the use of these in concert can provide information needed to navigate through cancer disease management challenges

UV activation of polymeric high aspect ratio microstructures: Ramifications in antibody surface loading for circulating tumor cell selection

The need to activate thermoplastic surfaces using robust and efficient methods has been driven by the fact that replication techniques can be used to produce microfluidic devices in a high production mode and at low cost, making polymer microfluidics invaluable for in vitro diagnostics, such as circulating tumor cell (CTC) analysis, where device disposability is critical to mitigate artifacts associated with sample carryover. Modifying the surface chemistry of thermoplastic devices through activation techniques can be used to increase the wettability of the surface or to produce functional scaffolds to allow for the covalent attachment of biologics, such as antibodies for CTC recognition. Extensive surface characterization tools were used to investigate UV activation of various surfaces to produce uniform and high surface coverage of functional groups, such as carboxylic acids in microchannels of different aspect ratios. We found that the efficiency of the UV activation process is highly dependent on the microchannel aspect ratio and the identity of the thermoplastic substrate. Colorimetric assays and fluorescence imaging of UV-activated microchannels following EDC/NHS coupling of Cy3-labeled oligonucleotides indicated that UV-activation of a PMMA microchannel with an aspect ratio of ???3 was significantly less efficient toward the bottom of the channel compared to the upper sections. This effect was a consequence of the bulk polymer's damping of the modifying UV radiation due to absorption artifacts. In contrast, this effect was less pronounced for COC. Moreover, we observed that after thermal fusion bonding of the device's cover plate to the substrate, many of the generated functional groups buried into the bulk rendering them inaccessible. The propensity of this surface reorganization was found to be higher for PMMA compared to COC. As an example of the effects of material and microchannel aspect ratios on device functionality, thermoplastic devices for the selection of CTCs from whole blood were evaluated, which required the immobilization of monoclonal antibodies to channel walls. From our results, we concluded the CTC yield and purity of isolated CTCs were dependent on the substrate material with COC producing the highest clinical yields for CTCs as well as better purities compared to PMMA.close9

Physics searches at the LHC

With the LHC up and running, the focus of experimental and theoretical high energy physics will soon turn to an interpretation of LHC data in terms of the physics of electroweak symmetry breaking and the TeV scale. We present here a broad review of models for new TeV-scale physics and their LHC signatures. In addition, we discuss possible new physics signatures and describe how they can be linked to specific models of physics beyond the Standard Model. Finally, we illustrate how the LHC era could culminate in a detailed understanding of the underlying principles of TeV-scale physics.Comment: 184 pages, 55 figures, 14 tables, hundreds of references; scientific feedback is welcome and encouraged. v2: text, references and Overview Table added; feedback still welcom

Contract, Conflict and Cooperation: A Critical Analysis of the Common Law Approach to the Breakdown of Modern, Complex, Symbiotic Contracts

The springboard of a real-world, doctrinal, and theoretical investigation of the role played by cooperation in complex modern contracts allows me to articulate and justify a deep and concrete Transcendent Duty to Cooperate (TDTC) for these contracts. The source of the TDTC is the express words and/or the background of such contracts, the commercial expectations of the parties; which reveal that successful performance re-quires cooperation. The inevitable inference from this is that parties implicitly agree to cooperate. As the duty is implicit, it follows, I argue, that there are no gaps to be filled; merely meaning to be unearthed from the words and/or the background (construction). In doctrinal work, I review cases in categories (prevention, facilitation, defect-rectification, communication, decision-making, and active cooperation), showing that the law is far from coherent but also far from incoherent. Shifting from judicial policy making and gap-filling to context/purpose based contract construction, using evidence, is possible and would provide coherence. I create a clear and enforceable definition of cooperation through analysing the opinions of around five-hundred commercial experts and synthesising those with doctrine and theory. My empirical work analyses experts’ views; collected by interview, an online survey and workshops, using vignettes developed from adjudicated/real-life cases including opinion on what cooperation is and how it is achieved. The findings of my survey are compared with others. At an abstract level, it aligns with comparable surveys and at a detailed level, it is unique. In theoretical work, I show that basing the TDTC on construction is superior and more efficient, brings coherence to the law and that it is underpinned by shared, normative, “community” values. I test the TDTC against various “hard” cases, analysing remedial issues, showing that it would not decrease certainty in English Commercial Law, and is defensible by an appeal for coherence

An Automated Enzymatic Inulin Assay, Capable of Full Sinistrin Hydrolysis

Peer Reviewe