Utilización de la red social Instagram para fomentar la práctica continuada del alumnado en la nomenclatura y formulación de compuestos orgánicos

Introducción El aprendizaje de la formulación y nomenclatura de compuestos orgánicos constituye una de las competencias más importantes en la asignatura de Química Orgánica I. Para ello, la resolución de ejercicios constituye una herramienta fundamental que actualmente se integra en el aula. Sin embargo, el grado de implicación del alumnado en la realización de estos ejercicios es, en muchas ocasiones, escaso, lo que lleva a que exista un alto porcentaje de alumnos y alumnas que tienen dificultades para aprobar satisfactoriamente esta parte de la materia. Objetivo La implementación de un Proyecto de Innovación Docente (PID) que propone la creación de un recurso didáctico para mejorar la adquisición de las competencias relativas a formulación orgánica y fomentar la implicación del alumnado de forma interactiva mediante el empleo de la red social Instagram. Metodología A partir de un banco de preguntas previamente elaborado por los integrantes del equipo docente, se prepararon una serie de cuestionarios que fueron compartidos a modo de stories en el perfil de una cuenta de Instagram creada a tal fin. Estos cuestionarios proporcionaron al alumnado una retroalimentación instantánea al completar el ejercicio, mostrando la respuesta correcta así como una explicación acorde con las reglas de la IUPAC. Resultados El impacto de la intervención fue evaluado tanto cualitativamente, mediante encuestas de percepción, como cuantitativamente, mediante el análisis de los datos derivados de la plataforma Instagram, así como de las calificaciones obtenidas en los exámenes de formulación parcial, final y extraordinario, mostrando una alta aceptación por parte del alumnado y una mejora de los resultados académicos. Conclusiones Instagram es una herramienta útil para la práctica de la formulación en la asignatura de Química Orgánica I que mejora la eficacia del proceso de transferencia de información entre profesor y alumno/a. La implantación de este recurso proporciona una herramienta atractiva e innovadora que, al mismo tiempo, refuerza los conocimientos adquiridos en clase en un contexto más cercano y mejora los resultados académicos

Selective Anticancer Therapy Based on a HA-CD44 Interaction Inhibitor Loaded on Polymeric Nanoparticles

This research was funded by the Consejeria de Economia, Conocimiento, Empresas y Universidad of the Junta de Andalucia (grant number Excellence Research Project P18-RT-1679) and the Research Results Transfer Office (OTRI) of the University of Granada (grant number PR/17/006 project). This work was partially supported by grants from the Spanish Ministry of Economy and Competitiveness (MINECO), grant number PID2019.110987RB.I00; the Health Institute Carlos III (ISCIII), grant number DTS18/00121 the Junta de Andalucia-FEDER, Ministry of Economy, Knowledge, Companies, and University (FEDER 2018: ref. B-FQM-475-UGR18, PAIDI2020: ref. PT18-TP-4160); and the Andalusian Regional Government, grant number PAIDI-TC-PVT-PSETC-2.0. C.D. thanks HECBioSim, the UK High End Computing Consortium for Biomolecular Simulation (hecbiosim.ac.uk), which is supported by the EPSRC (EP/L000253/1) for awarding computing time in Jade, a UK Tier-2 resource. B.R.-R. gratefully acknowledges funding from the European Union's Horizon 2020 Research and Innovation Program under Marie Sklodowska-Curie Grant Agreement no. 754446 and UGR Research and Knowledge Transfer Fund-Athenea3i. J.M.E.-R. thanks the Spanish Ministry of Education for PhD funding (scholarship FPU 16/02061). V.C.-C. thanks the Andalusian Regional Government for her postdoctoral fellowship (POSTDOC_21_00118).Hyaluronic acid (HA), through its interactions with the cluster of differentiation 44 (CD44), acts as a potent modulator of the tumor microenvironment, creating a wide range of extracellular stimuli for tumor growth, angiogenesis, invasion, and metastasis. An innovative antitumor treatment strategy based on the development of a nanodevice for selective release of an inhibitor of the HACD44 interaction is presented. Computational analysis was performed to evaluate the interaction of the designed tetrahydroisoquinoline-ketone derivative (JE22) with CD44 binding site. Cell viability, efficiency, and selectivity of drug release under acidic conditions together with CD44 binding capacity, effect on cell migration, and apoptotic activity were successfully evaluated. Remarkably, the conjugation of this CD44 inhibitor to the nanodevice generated a reduction of the dosis required to achieve a significant therapeutic effect.Junta de Andalucia P18-RT-1679Research Results Transfer Office (OTRI) of the University of Granada PR/17/006Spanish Government PID2019.110987RB.I00Health Institute Carlos III (ISCIII) DTS18/00121Junta de Andalucia-FEDER, Ministry of Economy, Knowledge, Companies, and University (FEDER) B-FQM-475-UGR18 PT18-TP-4160Andalusian Regional Government POSTDOC_21_00118UK Research & Innovation (UKRI)Engineering & Physical Sciences Research Council (EPSRC) EP/L000253/1European Union's Horizon 2020 Research and Innovation Program under Marie Sklodowska-Curie Grant 754446UGR Research and Knowledge Transfer Fund-Athenea3iSpanish Government FPU 16/0206

Lectura de contexto y abordaje psicosocial desde los enfoques narrativos Cartagena, Bogotá, Barranquilla, San Andrés Isla.

El presente trabajo tiene como propósito evidenciar el desarrollo de una propuesta metodológica mediante la cual se pretende que el profesional en formación de Psicología se apropie de los conocimientos para analizar y valorar eventos traumáticos con base en el enfoque narrativo a través de la revisión de relatos verídicos y utilizando como recurso complementario en esta temática el artículo de Michel White pionero de la terapia narrativa y titulado: El Trabajo con Personas que Sufren las Consecuencias de Trauma Múltiple: Una Perspectiva Narrativa. El texto fue publicado en el libro Recursos psicosociales para el posconflicto. El profesional en Psicología debe contar con las competencias, la argumentación teórica, las técnicas y herramientas para analizar, diagnosticar e intervenir en determinadas situaciones. En este sentido, la técnica de Análisis del Relato nos permite identificar diferentes expresiones, imágenes, símbolos, emociones y sentimientos de quien narra una situación que genera un trauma significativo y en muchos casos recurrente.The purpose of this paper is to demonstrate the development of a methodological proposal through which it is intended that the professional in psychology training appropriates the knowledge to analyze and assess traumatic events based on the narrative approach through the review of true stories and using as a complementary resource in this topic the article by Michel White, pioneer of narrative therapy and entitled: Working with People Suffering the Consequences of Multiple Trauma: A Narrative Perspective. The text was published in the book Psychosocial Resources for the Post-Conflict. The professional in Psychology must have the skills, the theoretical argumentation, the techniques and tools to analyze, diagnose and intervene in certain situations. In this sense, the technique of Story Analysis allows us to identify different expressions, images, symbols, emotions and feelings of those who narrate a situation that generates a significant and in many cases recurrent trauma

An effective polymeric nanocarrier that allows for active targeting and selective drug delivery in cell coculture systems

In this manuscript, we report the development of a versatile, robust, and stable targeting nanocarrier for active delivery. This nanocarrier is based on bifunctionalized polymeric nanoparticles conjugated to a monoclonal antibody that allows for active targeting of either (i) a fluorophore for tracking or (ii) a drug for monitoring specific cell responses. This nanodevice can efficiently discriminate between cells in coculture based on the expression levels of cell surface receptors. As a proof of concept, we have demonstrated efficient delivery using a broadly established cell surface receptor as the target, the epidermal growth factor receptor (EGFR), which is overexpressed in several types of cancers. Additionally, a second validation of this nanodevice was successfully carried out using another cell surface receptor as the target, the cluster of differentiation 147 (CD147). Our results suggest that this versatile nanocarrier can be expanded to other cell receptors and bioactive cargoes, offering remarkable discrimination efficiency between cells with different expression levels of a specific marker. This work supports the ability of nanoplatforms to boost and improve the progress towards personalized medicine.Health Institute Carlos III (ISCIII) DTS18/00121Junta de Andalucia-FEDER, Ministry of Economy, Knowledge, Companies and University (University of Granada) B-FQM-475-UGR18 PT18-TP-1490 AT17_6096- OTRISpanish State Research Agency RED2018-102469-TFundación Benéfica Anticáncer San Francisco Javier y Santa CándidaSpanish Ministry of Economy and Competitiveness PTQ-16- 08597Spanish Government FPU 16/0206

Tracking cell proliferation using a nanotechnology based approach

yesTo develop an efficient nanotechnology fluorescence-based method to track cell proliferation to avoid the limitations of current cell-labeling dyes. Material & methods: Synthesis, PEGylation, bifunctionalization and labeling with a fluorophore (Cy5) of 200 nm polystyrene nanoparticles (NPs) were performed. These NPs were characterized and assessed for in vitro long-term monitoring of cell proliferation. Results: The optimization and validation of this method to track long-term cell proliferation assays have been achieved with high reproducibility, without cell cycle disruption. This method has been successfully applied in several adherent and suspension cells including hard-to-transfect cells and isolated human primary lymphocytes. Conclusion: A novel approach to track efficiently cellular proliferation by flow cytometry using fluorescence labeled NPs has been successfully developed

Novel bead-based platform for direct detection of unlabelled nucleic acids through Single Nucleobase Labelling

Over the last decade, circulating microRNAs have received attention as diagnostic and prognostic biomarkers. In particular, microRNA122 has been demonstrated to be an early and more sensitive indicator of drug-induced liver injury than the widely used biomarkers such as alanine aminotransferase and aspartate aminotransferase. Recently, microRNA122 has been used in vitro to assess the cellular toxicity of new drugs and as a biomarker for the development of a rapid test for drug overdose/liver damage. In this proof-of-concept study, we report a PCR-free and label-free detection method that has a limit of detection (3 standard deviations) of 15 fmoles of microRNA122, by integrating a dynamic chemical approach for "Single Nucleobase Labelling" with a bead-based platform (Luminex®) thereby, in principle, demonstrating the exciting prospect of rapid and accurate profiling of any microRNAs related to diseases and toxicology

The evolution of the ventilatory ratio is a prognostic factor in mechanically ventilated COVID-19 ARDS patients

Background: Mortality due to COVID-19 is high, especially in patients requiring mechanical ventilation. The purpose of the study is to investigate associations between mortality and variables measured during the first three days of mechanical ventilation in patients with COVID-19 intubated at ICU admission. Methods: Multicenter, observational, cohort study includes consecutive patients with COVID-19 admitted to 44 Spanish ICUs between February 25 and July 31, 2020, who required intubation at ICU admission and mechanical ventilation for more than three days. We collected demographic and clinical data prior to admission; information about clinical evolution at days 1 and 3 of mechanical ventilation; and outcomes. Results: Of the 2,095 patients with COVID-19 admitted to the ICU, 1,118 (53.3%) were intubated at day 1 and remained under mechanical ventilation at day three. From days 1 to 3, PaO2/FiO2 increased from 115.6 [80.0-171.2] to 180.0 [135.4-227.9] mmHg and the ventilatory ratio from 1.73 [1.33-2.25] to 1.96 [1.61-2.40]. In-hospital mortality was 38.7%. A higher increase between ICU admission and day 3 in the ventilatory ratio (OR 1.04 [CI 1.01-1.07], p = 0.030) and creatinine levels (OR 1.05 [CI 1.01-1.09], p = 0.005) and a lower increase in platelet counts (OR 0.96 [CI 0.93-1.00], p = 0.037) were independently associated with a higher risk of death. No association between mortality and the PaO2/FiO2 variation was observed (OR 0.99 [CI 0.95 to 1.02], p = 0.47). Conclusions: Higher ventilatory ratio and its increase at day 3 is associated with mortality in patients with COVID-19 receiving mechanical ventilation at ICU admission. No association was found in the PaO2/FiO2 variation

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements