321 research outputs found
Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain
Aims: To identify the nutrients that can trigger the loss of flocculation under growth conditions in an ale-brewing strain, Saccharomyces cerevisiae NCYC 1195.
Methods and Results: Flocculation was evaluated using the method of Soares, EX. and Vroman, A. [Journal of Applied Microbiology (2003) 95, 325]. Yeast growth with metabolizable carbon sources (glucose, fructose, galactose, maltose or sucrose) at 2% (w/v), induced the loss of flocculation in yeast that had previously been allowed to flocculate. The yeast remained flocculent when transferred to a medium containing the required nutrients for yeast growth and a sole nonmetabolizable carbon source (lactose). Transfer of flocculent yeast into a growth medium with ethanol (4% v/v), as the sole carbon source did not induce the loss of flocculation. Even the addition of glucose (2% w/v) or glucose and antimycin A (0.1 mg lˉ¹) to this culture did not bring about loss of flocculation. Cycloheximide addition (15 mglˉ¹) to glucose-growing cells stopped flocculation loss.
Conclusions: Carbohydrates were the nutrients responsible for stimulating the loss of flocculation in flocculent yeast cells transferred to growing conditions. The glucose-induced loss of flocculation required de novo protein synthesis. Ethanol prevented glucose-induced loss of flocculation. This protective effect of ethanol was independent of the respiratory function of the yeast.
Significance and Impact of the Study: This work contributes to the elucidation of the role of nutrients in the control of the flocculation cycle in NewFlo phenotype yeast strains.Instituto Politécnico do Porto (IPP) - Fundo de Apoio à Investigação - Project P24/96 , P24/97.Programa Plurianual de Unidades de I&D-CIEA/
ISEP
D-term Inflation in Superstring Theories
An inflationary stage dominated by a -term avoids the slow-roll problem of
inflation in supergravity and may emerge in theories with a non-anomalous or
anomalous U(1) gauge symmetry. The most intriguing and commonly invoked
possibility is that the Fayet-Iliopoulos -term triggering inflation is the
one emerging in superstring theories. We discuss the complications one has to
face when trying to build up a successful -term inflationary scenario in
superstring models. In particular, we show that the ``vacuum shifting''
phenomenon of string theories is usually very efficient even in the early
Universe, thus preventing inflation from taking place. On the other hand, when
-term inflation is free to occur, the presence of a plethora of fields and
several non-anomalous additional abelian symmetries in string theories may help
in reconciling the value of the Fayet-Iliopoulos -term required by the COBE
normalization with the value predicted by string theories. We also show that in
superstring -term inflation gravitinos are likely to pose no cosmological
problem.Comment: 19 pages, LaTeX fil
Particle physics models of inflation
Inflation models are compared with observation on the assumption that the
curvature perturbation is generated from the vacuum fluctuation of the inflaton
field. The focus is on single-field models with canonical kinetic terms,
classified as small- medium- and large-field according to the variation of the
inflaton field while cosmological scales leave the horizon. Small-field models
are constructed according to the usual paradigm for beyond Standard Model
physicsComment: Based on a talk given at the 22nd IAP Colloquium, ``Inflation +25'',
Paris, June 2006 Curve omitted from final Figur
Flocculation onset in Saccharomyces cerevisiae: effect of ethanol, heat and osmotic stress
Aims: To examine the effect of different stress conditions on the onset of flocculation
in an ale-brewing strain, Saccharomyces cerevisiae NCYC 1195.
Methods and Results: Flocculation was evaluated using the method of Soares,
E.V. and Vroman, A. [Journal of Applied Microbiology (2003) 95, 325]; plasma
membrane integrity was accessed using propidium iodide and the staining of
the yeast cell wall was performed using calcofluor white M2R. Cells in exponential
phase of growth were subjected to different stress conditions. The addition
of 1%, 3% and 5% (v/v) ethanol, 1% and 3% (v/v) isopropanol or a brief heat shock (52ºC, 5 min), did not induce an early flocculation phenotype when compared with control cells. The addition of 10% (v/v) ethanol, a continuous mild heat-stress (37ºC) or an osmotic stress (0.5 or 1 mol l-1 of NaCl) did not induce a flocculent phenotype.
Conclusions: Flocculation seems not to be induced as a response to different
chemical (ethanol and isopropanol) and physical (heat and osmotic) stress conditions.
Conversely, osmotic and ethanol [10% (v/v)] stress, as well as a continuous mild heat shock (37ºC), have a negative impact on the phenotype expression of flocculation.
Significance and Impact of the Study: The findings reported here contribute to
the elucidation of the control of yeast flocculation. This information might be
useful to the brewing industry, as the time when the onset of flocculation
occurs can determine the fermentation performance and the beer quality, as
well as in other biotechnological industries where flocculation can be used as a
cell separation process.ERASMUS; ISEP (Portugal)
Weak magnetism and non-Fermi liquids near heavy-fermion critical points
This paper is concerned with the weak-moment magnetism in heavy-fermion
materials and its relation to the non-Fermi liquid physics observed near the
transition to the Fermi liquid. We explore the hypothesis that the primary
fluctuations responsible for the non-Fermi liquid physics are those associated
with the destruction of the large Fermi surface of the Fermi liquid. Magnetism
is suggested to be a low-energy instability of the resulting small Fermi
surface state. A concrete realization of this picture is provided by a
fractionalized Fermi liquid state which has a small Fermi surface of conduction
electrons, but also has other exotic excitations with interactions described by
a gauge theory in its deconfined phase. Of particular interest is a
three-dimensional fractionalized Fermi liquid with a spinon Fermi surface and a
U(1) gauge structure. A direct second-order transition from this state to the
conventional Fermi liquid is possible and involves a jump in the electron Fermi
surface volume. The critical point displays non-Fermi liquid behavior. A
magnetic phase may develop from a spin density wave instability of the spinon
Fermi surface. This exotic magnetic metal may have a weak ordered moment
although the local moments do not participate in the Fermi surface.
Experimental signatures of this phase and implications for heavy-fermion
systems are discussed.Comment: 20 pages, 8 figures; (v2) includes expanded discussion and solution
of quantum Boltzmann equatio
A comment on the PCAST report:skip the “match”/“non-match” stage
This letter comments on the report “Forensic science in criminal courts: Ensuring scientific validity of feature-comparison methods” recently released by the President's Council of Advisors on Science and Technology (PCAST). The report advocates a procedure for evaluation of forensic evidence that is a two-stage procedure in which the first stage is “match”/“non-match” and the second stage is empirical assessment of sensitivity (correct acceptance) and false alarm (false acceptance) rates. Almost always, quantitative data from feature-comparison methods are continuously-valued and have within-source variability. We explain why a two-stage procedure is not appropriate for this type of data, and recommend use of statistical procedures which are appropriate
Effect of the 3′-Terminal Truncation of the Human interferon-Gamma Gene on Plasmid Segregation in Escherichia Coli
FGF receptor genes and breast cancer susceptibility: results from the Breast Cancer Association Consortium
Background:Breast cancer is one of the most common malignancies in women. Genome-wide association studies have identified FGFR2 as a breast cancer susceptibility gene. Common variation in other fibroblast growth factor (FGF) receptors might also modify risk. We tested this hypothesis by studying genotyped single-nucleotide polymorphisms (SNPs) and imputed SNPs in FGFR1, FGFR3, FGFR4 and FGFRL1 in the Breast Cancer Association Consortium.
Methods:Data were combined from 49 studies, including 53 835 cases and 50 156 controls, of which 89 050 (46 450 cases and 42 600 controls) were of European ancestry, 12 893 (6269 cases and 6624 controls) of Asian and 2048 (1116 cases and 932 controls) of African ancestry. Associations with risk of breast cancer, overall and by disease sub-type, were assessed using unconditional logistic regression.
Results:Little evidence of association with breast cancer risk was observed for SNPs in the FGF receptor genes. The strongest evidence in European women was for rs743682 in FGFR3; the estimated per-allele odds ratio was 1.05 (95 confidence interval=1.02-1.09, P=0.0020), which is substantially lower than that observed for SNPs in FGFR2.
Conclusion:Our results suggest that common variants in the other FGF receptors are not associated with risk of breast cancer to the degree observed for FGFR2. © 2014 Cancer Research UK
Search for direct production of charginos and neutralinos in events with three leptons and missing transverse momentum in √s = 7 TeV pp collisions with the ATLAS detector
A search for the direct production of charginos and neutralinos in final states with three electrons or muons and missing transverse momentum is presented. The analysis is based on 4.7 fb−1 of proton–proton collision data delivered by the Large Hadron Collider and recorded with the ATLAS detector. Observations are consistent with Standard Model expectations in three signal regions that are either depleted or enriched in Z-boson decays. Upper limits at 95% confidence level are set in R-parity conserving phenomenological minimal supersymmetric models and in simplified models, significantly extending previous results
Jet size dependence of single jet suppression in lead-lead collisions at sqrt(s(NN)) = 2.76 TeV with the ATLAS detector at the LHC
Measurements of inclusive jet suppression in heavy ion collisions at the LHC
provide direct sensitivity to the physics of jet quenching. In a sample of
lead-lead collisions at sqrt(s) = 2.76 TeV corresponding to an integrated
luminosity of approximately 7 inverse microbarns, ATLAS has measured jets with
a calorimeter over the pseudorapidity interval |eta| < 2.1 and over the
transverse momentum range 38 < pT < 210 GeV. Jets were reconstructed using the
anti-kt algorithm with values for the distance parameter that determines the
nominal jet radius of R = 0.2, 0.3, 0.4 and 0.5. The centrality dependence of
the jet yield is characterized by the jet "central-to-peripheral ratio," Rcp.
Jet production is found to be suppressed by approximately a factor of two in
the 10% most central collisions relative to peripheral collisions. Rcp varies
smoothly with centrality as characterized by the number of participating
nucleons. The observed suppression is only weakly dependent on jet radius and
transverse momentum. These results provide the first direct measurement of
inclusive jet suppression in heavy ion collisions and complement previous
measurements of dijet transverse energy imbalance at the LHC.Comment: 15 pages plus author list (30 pages total), 8 figures, 2 tables,
submitted to Physics Letters B. All figures including auxiliary figures are
available at
http://atlas.web.cern.ch/Atlas/GROUPS/PHYSICS/PAPERS/HION-2011-02
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