RUNX3 Regulates Intercellular Adhesion Molecule 3 (ICAM-3) Expression during Macrophage Differentiation and Monocyte Extravasation

The adhesion molecule ICAM-3 belongs to the immunoglobulin gene superfamily and functions as a ligand for the β2 integrins LFA-1, Mac-1 and αdβ2. The expression of ICAM-3 is restricted to cells of the hematopoietic lineage. We present evidences that the ICAM-3 gene promoter exhibits a leukocyte-specific activity, as its activity is significantly higher in ICAM-3+ hematopoietic cell lines. The activity of the ICAM-3 gene promoter is dependent on the occupancy of RUNX cognate sequences both in vitro and in vivo, and whose integrity is required for RUNX responsiveness and for the cooperative actions of RUNX with transcription factors of the Ets and C/EBP families. Protein analysis revealed that ICAM-3 levels diminish upon monocyte-derived macrophage differentiation, monocyte transendothelial migration and dendritic cell maturation, changes that correlate with an increase in RUNX3. Importantly, disruption of RUNX-binding sites led to enhanced promoter activity, and small interfering RNA-mediated reduction of RUNX3 expression resulted in increased ICAM-3 mRNA levels. Altogether these results indicate that the ICAM-3 gene promoter is negatively regulated by RUNX transcription factors, which contribute to the leukocyte-restricted and the regulated expression of ICAM-3 during monocyte-to-macrophage differentiation and monocyte extravasation

Timing and severity of inhibitor development in recombinant versus plasma-derived factor VIII concentrates: a SIPPET analysis

Essentials Recombinant factor VIII (rFVIII) was contrasted with plasma-derived FVIII (pdFVIII). In previously untreated patients with hemophilia A, rFVIII led to more inhibitors than pdFVIII. Inhibitors with rFVIII developed earlier, and the peak rate was higher than with pdFVIII. Inhibitors with rFVIII were more severe (higher titre) than with pdFVIII. Summary: Background The development of neutralizing antibodies (inhibitors) against factor VIII (FVIII) is the most severe complication in the early phases of treatment of severe hemophilia A. Recently, a randomized trial, the Survey of Inhibitors in Plasma-Product Exposed Toddlers (SIPPET) demonstrated a 2-fold higher risk of inhibitor development in children treated with recombinant FVIII (rFVIII) products than with plasma-derived FVIII (pdFVIII) during the first 50 exposure days (EDs). Objective/Methods In this post-hoc SIPPET analysis we evaluated the rate of inhibitor incidence over time by every 5 EDs (from 0 to 50 EDs) in patients treated with different classes of FVIII product, made possible by a frequent testing regime. Results The highest rate of inhibitor development occurred in the first 10 EDs, with a large contrast between rFVIII and pdFVIII during the first 5 EDs: hazard ratio 3.14 (95% confidence interval [CI], 1.01\ue2\u80\u939.74) for all inhibitors and 4.19 (95% CI, 1.18\ue2\u80\u9314.8) for high-titer inhibitors. For patients treated with pdFVIII, the peak of inhibitor development occurred later (6\ue2\u80\u9310 EDs) and lasted for a shorter time. Conclusion These results emphasize the high immunologic vulnerability of patients during the earliest exposure to FVIII concentrates, with the strongest response to recombinant FVIII products

Overview of the DESI Legacy Imaging Surveys

The DESI Legacy Imaging Surveys (http://legacysurvey.org/) are a combination of three public projects (the Dark Energy Camera Legacy Survey, the Beijing–Arizona Sky Survey, and the Mayall z-band Legacy Survey) that will jointly image ≈14,000 deg2 of the extragalactic sky visible from the northern hemisphere in three optical bands (g, r, and z) using telescopes at the Kitt Peak National Observatory and the Cerro Tololo Inter-American Observatory. The combined survey footprint is split into two contiguous areas by the Galactic plane. The optical imaging is conducted using a unique strategy of dynamically adjusting the exposure times and pointing selection during observing that results in a survey of nearly uniform depth. In addition to calibrated images, the project is delivering a catalog, constructed by using a probabilistic inference-based approach to estimate source shapes and brightnesses. The catalog includes photometry from the grz optical bands and from four mid-infrared bands (at 3.4, 4.6, 12, and 22 μm) observed by the Wide-field Infrared Survey Explorer satellite during its full operational lifetime. The project plans two public data releases each year. All the software used to generate the catalogs is also released with the data. This paper provides an overview of the Legacy Surveys project

Efectividad de la acupuntura en pacientes con epicondilalgia lateral: un resumen de revisiones sistemáticas

Introduction: Epicondylalgia lateral skeletal muscle is a fairly common condition, characterized by the presence of pain, decreased grip strength and progressive loss of function of the hand. Objective: To summarize the evidence in Cochrane and non-Cochrane, looking at the effects and bene ts of acupuncture for the treatment of pain in patients who may be LE. Methods: A search was con- ducted in the specialized registry of the Cochrane musculoskeletal group (1980-September 2015) and databases: Medline (1980-September 2015), Pedro (1980-September 2015), Metasearcher Epistemonikos (1980-September 2015), gray literature, conference proceedings was sought. The methodological quality of systematic reviews was evaluated including amstar and the overall quality of the evidence using grade. Results: The search identified 92 preliminary systematic reviews, of which two met the eligibility criteria. 2 systematic reviews were categorized as high methodological quality. Conclusion: Despite the rarity of prescription and their application in subjects with lateral epicondylalgia, acupuncture has been proven effective in relieving pain in the short term.A epicondilalgia lateral é uma condição musculoesquelética bastante comum, que se caracteriza pela presença de dor, diminuição na força de preensão e perda progressiva da funcionalidade da mão. A acupuntura é um método terapêutico derivado da medicina chinesa tradicional, onde se realiza estimulação de pontos corporais mediante guias, com  ns terapêuticos ou preventivos. Alguns estudos têm reportado que a acupuntura produz um benefício a curto prazo sobre a dor localizada no epicôndilo lateral do cotovelo; no entanto, os benefícios que informam os estudos associados a esta patologia não conseguem ser concluintes. Objetivo: Resumir a evidência existente em revisões Cochrane e não Cochrane, buscando os efeitos e benefícios da Acupuntura para o tratamento da dor em sujeitos que apresentem Epicondilalgia lateral. Materiais e métodos: Realizou-se uma busca no registro especializado Cochrane do grupo musculoesquelético (1980- Junho 2015) e nas bases de dados: Medline (1980-Setembro 2015), PEDro (1980- Setembro 2015), Metabuscador Epistemonikos (1980- Setembro 2015), se buscou literatura cinzenta e atas de con- gressos. A qualidade metodológica das revisões sistemáticas incluídas foi avaliada com AMSTAR e a qualidade global da evidência mediante GRADE. Resultados: A busca preliminar identi cou 92 revisões sistemáticas, das quais 2 cumpriram com os critérios de elegibilidade. As 2 revisões sistemáticas foram categorizadas como de alta qualidade metodológica. Conclusão: Apesar da prescrição e a sua aplicação em sujeitos que apresentam epicondilalgia lateral, a acupuntura tem demonstrado ser e caz no alívio da dor a curto prazo.La epicondilalgia lateral es una condición músculoesquelética bastante común, que se caracteriza por la presencia de dolor, disminución en la fuerza de prensión y pérdida progresiva de la funcionalidad de la mano. La acupuntura es un método terapéutico derivado de la medicina china tradicional, en donde se realiza estimulación de puntos corporales mediante agujas, con nes terapéuticos o preventivos. Algunos estudios han reportado que la acupuntura produce un beneficio a corto plazo sobre el dolor localizado en el epicóndilo lateral del codo; sin embargo, los beneficios que informan los estudios asociados a esta patología no logran ser concluyentes. Objetivo: resumir la evidencia existente en revisiones Cochrane y no Cochrane, buscando los efectos y bene cios de la acupuntura para el tratamiento del dolor en sujetos que presenten epicondilalgia lateral. Materiales y métodos: se realizó una búsqueda en el registro especializado Cochrane del grupo músculo-esquelético (1980-junio 2015) y en las bases de datos: Medline (1980-septiembre 2015), PEDro (1980- septiembre 2015), Metabuscador Epistemonikos (1980- septiembre 2015), se buscó literatura gris y actas de congresos. La calidad metodológica de las revisiones sistemáticas incluidas fueron evaluadas con amstar y la calidad global de la evidencia mediante grade. Resultados: La búsqueda preliminar identicó 92 revisiones sistemáticas, de las cuales 2 cumplieron con los criterios de elegibilidad. Las 2 revisiones sistemáticas fueron categorizadas como de alta calidad metodológica. Conclusión: a pesar de lo poco común de la prescripción y su aplicación sujetos que presentan epicondilalgia lateral, la acupuntura ha demostrado ser eficaz en el alivio del dolor a corto plazo

Reproductive period and sexual maturity of the sardine Sardina pilchardus (Walbaum, 1792) in Gran Canaria waters (Canary Islands)

The present paper discusses the reproductive period and sexual maturity of a sample of sardine Sardina pilchardus (Walbaum, 1792) (N = 831) caught off the south-west coast of Gran Canaria (Canary Islands) from January 1995 to May 1996. The size range of the fish was 4.9-23.2 cm total length, and weight ranged from 0.8 to 101.5 g. Regarding sex ratio, there was no significant difference in the number of males and females. The reproductive period extended from November to March, with most gonadal activity in January-February. Mean total lengths at 50 % and 95 % of maturity were 15.0 cm and 17.8 cm, respectively, for males, and 15.2 cm and 18.4 cm for females.Se estudian 831 ejemplares de sardina Sardina pilchardus (Walbaum, 1792) capturados al suroeste de la isla de Gran Canaria (islas Canarias) durante el periodo comprendido entre enero de 1995 y mayo de 1996. El rango de tallas está comprendido entre 4,9 cm y 23,2 cm de longitud total y el de pesos entre 0,8 g y 101,5 g. La proporción entre machos y hembras no presenta diferencias significativas de la relación teórica 1:1. El periodo reproductor se extiende entre los meses de noviembre y marzo, con un máximo de actividad gonadal en enero-febrero. Las tallas medias de primera madurez sexual y de maduración masiva se alcanzan respectivamente a los 15,0 cm y 17,8 cm de longitud total en los machos y a los 15,2 cm y 18,4 cm en las hembras.Instituto Español de Oceanografí

Comparison of Oil Content and Fatty Acids Profile of Western Schley, Wichita, and Native Pecan Nuts Cultured in Chihuahua, Mexico

Two different extraction processes, Soxhlet and ultrasound, were used to obtain the oil extracts of Western Schley, Wichita, and Native pecan nuts cultured in Chihuahua, Mexico. The aspects evaluated in this study were the extraction yield of the processes and fatty acids’ profile of the resulting extracts. Gas chromatography coupled with mass spectrometry (GC-MS) was used to identify and determine the composition percentage of fatty acids present in pecan nuts oils extracted. The results obtained show that higher oil extraction yields were obtained by Soxhlet method with hexane (69.90%) in Wichita varieties. Wichita, Western Schley, and Native pecan nuts from Chihuahua are rich in PUFA (polyunsaturated fatty acids) and MUFA (monounsaturated fatty acids) and have low levels of SFA (saturated fatty acids). The predominant fatty acid present in all pecan nuts oils was linoleic acid followed by oleic acid. Myristic acid, palmitic acid, and linolenic acid were also identified in representative quantities. The results from this study suggest that there are statistically significant differences in the chemical composition of the pecan nuts oils extracted from the varieties cultured in Chihuahua, Mexico, and those cultivated in other regions of the world

Comparison of Oil Content and Fatty Acids Profile of Western Schley, Wichita, and Native Pecan Nuts Cultured in Chihuahua, Mexico

Two different extraction processes, Soxhlet and ultrasound, were used to obtain the oil extracts of Western Schley, Wichita, and Native pecan nuts cultured in Chihuahua, Mexico. The aspects evaluated in this study were the extraction yield of the processes and fatty acids’ profile of the resulting extracts. Gas chromatography coupled with mass spectrometry (GC-MS) was used to identify and determine the composition percentage of fatty acids present in pecan nuts oils extracted. The results obtained show that higher oil extraction yields were obtained by Soxhlet method with hexane (69.90%) in Wichita varieties. Wichita, Western Schley, and Native pecan nuts from Chihuahua are rich in PUFA (polyunsaturated fatty acids) and MUFA (monounsaturated fatty acids) and have low levels of SFA (saturated fatty acids). The predominant fatty acid present in all pecan nuts oils was linoleic acid followed by oleic acid. Myristic acid, palmitic acid, and linolenic acid were also identified in representative quantities. The results from this study suggest that there are statistically significant differences in the chemical composition of the pecan nuts oils extracted from the varieties cultured in Chihuahua, Mexico, and those cultivated in other regions of the world

Uso del taping en el manejo clínico de sujetos con pinzamiento sub-acromial: Revisión sistemática

The sub-acromial impingement (SAI) is the most common musculoskeletal disorder of shoulder pathologies. In recent times, the taping has become a relatively new and quite common indication in recent years. There will be a synthesis of evidence through a systematic review (SR) of randomized trials that had compared the use of taping for the management of these patients. The aim of this review is to determine the effect of taping on pain in subjects with sub-acromial pinch. The research strategy included randomized clinical trials (RCTs) databases were used: Medline, Cinahl, PEDro, Central, LILACS. In addition, we searched electronic journals, conference proceedings, randomized trials registry and contacted experts in the field. The results were two articles that met our eligibility criteria. We conclude that there is low quality evidence suggesting that the application of taping, would be effective to improve functionality and reduce pain at rest and during movements up to 1 week after application, in subjects with sub-acromial pinch compared to physical therapy program.El pinzamiento sub-acromial (SPS) es el trastorno músculo-esquelético más frecuente de las patologías de hombro. El taping se ha transformado en una indicación relativamente nueva y bastante común en los últimos años. Se realizará una síntesis de la evidencia a través de una revisión sistemática (RS) de ensayos clínicos aleatorizados que hayan comparado el uso del taping para el manejo de estos pacientes. El objetivo de esta revisión es determinar el efecto del taping sobre el dolor en sujetos con pellizcamiento sub-acromial. La estrategia de búsqueda incluyó ensayos clínicos aleatorizados (ECA); las bases de datos usadas fueron: Medline, Cinahl, PEDro, Central, LILACS. Además, se realizó una búsqueda en revistas electrónicas, actas de congresos, Registro de Estudios Clínicos Aleatorizados y se contactó con expertos en el tema. Los resultados obtenidos fueron de dos artículos que cumplían con nuestros criterios de elegibilidad. Se concluye que existe evidencia de baja calidad que sugiere que la aplicación de taping sería efectiva para mejorar la funcionalidad, reducir el dolor en reposo y durante los movimientos hasta en una semana posterior a su aplicación, en sujetos que presentan pellizcamiento sub-acromial en comparación a un programa de terapia física