The Brazilian grapevine variety called ‘Peverella’ corresponds to the ‘Boschera’ variety

Through fingerprinting analysis using reference microsatellite markers and comparing with the Vitis International Variety Catalogue (VIVC) database, it was possible to discover that in Brazil, the wine grape variety cultivated for over 70 years, called 'Peverella' (Variety number VIVC = 12963; National Register of Vine Varieties (Italy), code: 254) is actually the grape variety called 'Boschera' (Variety number VIVC = 1576;National Register of Vine Varieties (Italy), code: 326). This discovery makes wines produced in Brazil even rarer (with only approximately 10 hectares planted worldwide), presenting an additional means to promote and market this historic variety

Activity and consciousness: reflections on the teaching work from a cultural-historical perspective

The purpose of this theoretical article is to reflect on how teaching can be understood as a work activity under the referential of Cultural-Historical Psychology. From this perspective, we emphasize the concepts of activity and consciousness; a brief discussion on the psychological function of the teaching work is also presented. We argue that teachers' awareness of their work activity is a relevant tool that transforms teaching practices and strengthens their power to act in the situated context of the classroom. We conclude that Cultural-Historical Psychology brings significant contributions to the understanding of teaching activity, being configured as an approach that values the creative and critical character of teaching as a conscious, intentional, accessible, and transforming action of work and life relations for the teacher. O objetivo deste artigo, de caráter teórico, é refletir como a docência pode ser compreendida enquanto atividade de trabalho sob os referenciais da Psicologia Histórico-Cultural. Nessa perspectiva, enfatizam-se os conceitos de atividade e consciência; apresenta-se também uma breve discussão sobre a função psicológica do trabalho docente. Argumenta-se que a consciência do professor acerca de sua atividade de trabalho configura um importante instrumento que transforma as práticas docentes e reforça seu poder de agir no contexto situado da sala de aula. Conclui-se que a Psicologia Histórico-Cultural aporta significativas contribuições para a compreensão da atividade docente, configurando-se como uma abordagem que valoriza o caráter criativo e crítico da docência como ação consciente intencional, livre e transformadora das relações de trabalho e de vida para o professor

Inclusão no ensino superior: possibilidades docentes a partir da Teoria Histórico-Cultural

In view of the contemporary educational scenario in Brazil this article, of a reflective theoretical nature, seeks to problematize the different spheres of exclusion in Higher Education, emphasizing the possibilities of action/ transformation in this context. Based on the Historical-Cultural Theory, the article discusses the specificities of the inclusion and exclusion processes that are established in Higher Education and reflects on the teaching work, with a view to the consolidation of educational environments that are effectively inclusive in which it is possible to recognize, value and promote the presence, participation and learning of each student.En vista del escenario educativo contemporáneo en Brasil este artículo, de carácter teórico reflexivo, busca problematizar las diferentes esferas de exclusión en la Educación Superior, enfatizando las posibilidades de acción/ transformación docente en este contexto. A partir de la Teoría Histórico-Cultural, el artículo discute las especificidades de los procesos de inclusión y exclusión que se establecen en la Educación Superior y reflexiona sobre la labor docente, con miras a la consolidación de entornos educativos efectivamente inclusivos en los que se pueda reconocer, valorar y promover la presencia, participación y aprendizaje de cada alumno.Diante do cenário educacional contemporâneo no Brasil, este artigo, de cunho teórico reflexivo, tem por objetivo problematizar as diferentes esferas de exclusão no ensino superior, enfatizando as possibilidades de (transform)ação docente nesse contexto. Fundamentado na Teoria Histórico-Cultural, o artigo discute as especificidades dos processos de inclusão e exclusão que se estabelecem no ensino superior e tece reflexões sobre o trabalho docente, com vistas à consolidação de ambientes educacionais efetivamente inclusivos nos quais se possa reconhecer, valorizar e promover a presença, a participação e a aprendizagem de cada estudante

A Lactose-Binding Lectin from the Marine Sponge Cinachyrella Apion (Cal) Induces Cell Death in Human Cervical Adenocarcinoma Cells

Cancer represents a set of more than 100 diseases, including malignant tumors from different locations. Strategies inducing differentiation have had limited success in the treatment of established cancers. Marine sponges are a biological reservoir of bioactive molecules, especially lectins. Several animal and plant lectins were purified with antitumor activity, mitogenic, anti-inflammatory and antiviral, but there are few reports in the literature describing the mechanism of action of lectins purified from marine sponges to induce apoptosis in human tumor cells. In this work, a lectin purified from the marine sponge Cinachyrella apion (CaL) was evaluated with respect to its hemolytic, cytotoxic and antiproliferative properties, besides the ability to induce cell death in tumor cells. The antiproliferative activity of CaL was tested against HeLa, PC3 and 3T3 cell lines, with highest growth inhibition for HeLa, reducing cell growth at a dose dependent manner (0.5–10 µg/mL). Hemolytic activity and toxicity against peripheral blood cells were tested using the concentration of IC50 (10 µg/mL) for both trials and twice the IC50 for analysis in flow cytometry, indicating that CaL is not toxic to these cells. To assess the mechanism of cell death caused by CaL in HeLa cells, we performed flow cytometry and western blotting. Results showed that lectin probably induces cell death by apoptosis activation by pro-apoptotic protein Bax, promoting mitochondrial membrane permeabilization, cell cycle arrest in S phase and acting as both dependent and/or independent of caspases pathway. These results indicate the potential of CaL in studies of medicine for treating cancer

Discovery of Markers of Exposure Specific to Bites of Lutzomyia longipalpis, the Vector of Leishmania infantum chagasi in Latin America

Leishmania parasites are transmitted by the bite of an infected vector sand fly that injects salivary molecules into the host skin during feeding. Certain salivary molecules can produce antibodies and can be used as an indicator of exposure to a vector sand fly and potentially the disease it transmits. Here we identified potential markers of specific exposure to the sand fly Lutzomyia longipalpis, the vector of visceral leishmaniasis in Latin America. Initially, we determined which of the salivary proteins produce antibodies in humans, dogs, and foxes from areas endemic for the disease. To identify potential specific markers of vector exposure, we produced nine different recombinant salivary proteins from Lu. longipalpis and tested for their recognition by individuals exposed to another human-biting sand fly, Lu. intermedia, that transmits cutaneous leishmaniasis and commonly occurs in the same endemic areas as Lu. longipalpis. Two of the nine salivary proteins were recognized only by humans exposed to Lu. longipalpis, suggesting they are immunogenic proteins and may be useful in epidemiological studies. The identification of specific salivary proteins as potential markers of exposure to vector sand flies will increase our understanding of vector–human interaction, bring new insights to vector control, and in some instances act as an indicator for risk of acquiring disease

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Production of He-4 and (4) in Pb-Pb collisions at root(NN)-N-S=2.76 TeV at the LHC

Results on the production of He-4 and (4) nuclei in Pb-Pb collisions at root(NN)-N-S = 2.76 TeV in the rapidity range vertical bar y vertical bar <1, using the ALICE detector, are presented in this paper. The rapidity densities corresponding to 0-10% central events are found to be dN/dy4(He) = (0.8 +/- 0.4 (stat) +/- 0.3 (syst)) x 10(-6) and dN/dy4 = (1.1 +/- 0.4 (stat) +/- 0.2 (syst)) x 10(-6), respectively. This is in agreement with the statistical thermal model expectation assuming the same chemical freeze-out temperature (T-chem = 156 MeV) as for light hadrons. The measured ratio of (4)/He-4 is 1.4 +/- 0.8 (stat) +/- 0.5 (syst). (C) 2018 Published by Elsevier B.V.Peer reviewe

MUSiC : a model-unspecific search for new physics in proton-proton collisions at root s=13TeV

Results of the Model Unspecific Search in CMS (MUSiC), using proton-proton collision data recorded at the LHC at a centre-of-mass energy of 13 TeV, corresponding to an integrated luminosity of 35.9 fb(-1), are presented. The MUSiC analysis searches for anomalies that could be signatures of physics beyond the standard model. The analysis is based on the comparison of observed data with the standard model prediction, as determined from simulation, in several hundred final states and multiple kinematic distributions. Events containing at least one electron or muon are classified based on their final state topology, and an automated search algorithm surveys the observed data for deviations from the prediction. The sensitivity of the search is validated using multiple methods. No significant deviations from the predictions have been observed. For a wide range of final state topologies, agreement is found between the data and the standard model simulation. This analysis complements dedicated search analyses by significantly expanding the range of final states covered using a model independent approach with the largest data set to date to probe phase space regions beyond the reach of previous general searches.Peer reviewe

Measurement of prompt open-charm production cross sections in proton-proton collisions at root s=13 TeV

The production cross sections for prompt open-charm mesons in proton-proton collisions at a center-of-mass energy of 13TeV are reported. The measurement is performed using a data sample collected by the CMS experiment corresponding to an integrated luminosity of 29 nb(-1). The differential production cross sections of the D*(+/-), D-+/-, and D-0 ((D) over bar (0)) mesons are presented in ranges of transverse momentum and pseudorapidity 4 < p(T) < 100 GeV and vertical bar eta vertical bar < 2.1, respectively. The results are compared to several theoretical calculations and to previous measurements.Peer reviewe