129 research outputs found
Draft genome sequence of the N2-fixing cyanobacterium Nostoc piscinale CENA21, isolated from the Brazilian Amazon floodplain
We announce here the draft genome sequence of Nostoc piscinale CENA21, a diazotrophic heterocyst-forming cyanobacterium isolated from the Solimões River, Amazon Basin, Brazil. It consists of one circular chromosome scaffold with 11 contigs and total size of 7,094,556 bp. Secondary metabolite annotations indicate a good source for the discovery of novel natural products. © 2016 Leão et al.This work has been supported by grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and from the Funda ção de Amparo à Pesquisa do Estado do Pará (BIONORTE-CNPq/FAPESPA) to M.P.C.S. This work, including the efforts of Marli Fatima Fiore and Maria Paula Cruz Schneider, was funded by MCTI | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (307045/2010-2 and 306607/2012-3). This work, including the efforts of Tiago Leão and Pedro Ivo Guimaraes, was funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (13425-13-7 and 13194-13-5). This work, including the efforts of Pedro Nuno Leão, was funded by Ministry of Education and Science | Fundação para a Ciência e a Tecnologia (FCT) (IF/01358/2014)
Cyanobacterial diversity in microbial mats from the hypersaline lagoon system of Araruama, Brazil: An in-depth polyphasic study
Microbial mats are complex, micro-scale ecosystems that can be found in a wide range of environments. In the top layer of photosynthetic mats from hypersaline environments, a large diversity of cyanobacteria typically predominates. With the aim of strengthening the knowledge on the cyanobacterial diversity present in the coastal lagoon system of Araruama (state of Rio de Janeiro, Brazil), we have characterized three mat samples by means of a polyphasic approach. We have used morphological and molecular data obtained by culture-dependent and -independent methods. Moreover, we have compared different classification methodologies and discussed the outcomes, challenges, and pitfalls of these methods. Overall, we show that Araruama's lagoons harbor a high cyanobacterial diversity. Thirty-six unique morphospecies could be differentiated, which increases by more than 15% the number of morphospecies and genera already reported for the entire Araruama system. Morphology-based data were compared with the 16S rRNA gene phylogeny derived from isolate sequences and environmental sequences obtained by PCR-DGGE and pyrosequencing. Most of the 48 phylotypes could be associated with the observed morphospecies at the order level. More than one third of the sequences demonstrated to be closely affiliated (best BLAST hit results of ≥99%) with cyanobacteria from ecologically similar habitats. Some sequences had no close relatives in the public databases, including one from an isolate, being placed as "loner" sequences within different orders. This hints at hidden cyanobacterial diversity in the mats of the Araruama system, while reinforcing the relevance of using complementary approaches to study cyanobacterial diversity. © 2017 Ramos, Castelo-Branco, Leão, Martins, Carvalhal-Gomes, Sobrinho da Silva, Mendonça Filho and Vasconcelos.This work was supported by the Biogeochemical Project
(AMPETRO 14777—Cooperation term 0050.0023165.06.4)
of the GSE (Sedimentology Management) Network of
PETROBRAS, by the Brazilian National Research Agency—
CNPq and by the Research Agency of Rio de Janeiro
State—FAPERJ. It was also funded by Portuguese National
Funds through FCT—Fundação para a Ciência e a Tecnologia,
grants SFRH/BD/80153/2011 to VR, IF/01358/2014 to PL,
and UID/Multi/04423/2013 and by the Structured Program of
R&D&I INNOVMAR—Innovation and Sustainability in the
Management and Exploitation of Marine Resources (reference
NORTE-01-0145-FEDER-000035, Research Line NOVELMAR),
funded by the Northern Regional Operational Program
(NORTE2020) through the European Regional Development
Fund (ERDF)
Searching singlet extensions of the supersymmetric standard model in orbifold compactification of heterotic string
We search for supersymmetric standard model realizations with extra singlets
and extra using the heterotic string compactification on the orbifold with two Wilson lines. We analyze the vacuum restabilization
mechanism for three representative Pati-Salam string models obtained in the
literature and present detailed results for the effective superpotential
compatible with the string selection rules. An automated selection of
semi-realistic vacua along flat directions in the non-Abelian singlet modes
field space is performed by requiring the presence of massless pairs of
electroweak Higgs bosons having trilinear superpotential couplings with
massless singlet modes and the decoupling of color triplet exotic modes needed
to suppress and number violating processes.Comment: revtex4 format, 21 pages, 7 tables, shortened version added
reference
Population and fertility by age and sex for 195 countries and territories, 1950–2017: a systematic analysis for the Global Burden of Disease Study 2017
Background: Population estimates underpin demographic and epidemiological research and are used to track progress on numerous international indicators of health and development. To date, internationally available estimates of population and fertility, although useful, have not been produced with transparent and replicable methods and do not use standardised estimates of mortality. We present single-calendar year and single-year of age estimates of fertility and population by sex with standardised and replicable methods. Methods: We estimated population in 195 locations by single year of age and single calendar year from 1950 to 2017 with standardised and replicable methods. We based the estimates on the demographic balancing equation, with inputs of fertility, mortality, population, and migration data. Fertility data came from 7817 location-years of vital registration data, 429 surveys reporting complete birth histories, and 977 surveys and censuses reporting summary birth histories. We estimated age-specific fertility rates (ASFRs; the annual number of livebirths to women of a specified age group per 1000 women in that age group) by use of spatiotemporal Gaussian process regression and used the ASFRs to estimate total fertility rates (TFRs; the average number of children a woman would bear if she survived through the end of the reproductive age span [age 10–54 years] and experienced at each age a particular set of ASFRs observed in the year of interest). Because of sparse data, fertility at ages 10–14 years and 50–54 years was estimated from data on fertility in women aged 15–19 years and 45–49 years, through use of linear regression. Age-specific mortality data came from the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2017 estimates. Data on population came from 1257 censuses and 761 population registry location-years and were adjusted for underenumeration and age misreporting with standard demographic methods. Migration was estimated with the GBD Bayesian demographic balancing model, after incorporating information about refugee migration into the model prior. Final population estimates used the cohort-component method of population projection, with inputs of fertility, mortality, and migration data. Population uncertainty was estimated by use of out-of-sample predictive validity testing. With these data, we estimated the trends in population by age and sex and in fertility by age between 1950 and 2017 in 195 countries and territories. Findings: From 1950 to 2017, TFRs decreased by 49\ub74% (95% uncertainty interval [UI] 46\ub74–52\ub70). The TFR decreased from 4\ub77 livebirths (4\ub75–4\ub79) to 2\ub74 livebirths (2\ub72–2\ub75), and the ASFR of mothers aged 10–19 years decreased from 37 livebirths (34–40) to 22 livebirths (19–24) per 1000 women. Despite reductions in the TFR, the global population has been increasing by an average of 83\ub78 million people per year since 1985. The global population increased by 197\ub72% (193\ub73–200\ub78) since 1950, from 2\ub76 billion (2\ub75–2\ub76) to 7\ub76 billion (7\ub74–7\ub79) people in 2017; much of this increase was in the proportion of the global population in south Asia and sub-Saharan Africa. The global annual rate of population growth increased between 1950 and 1964, when it peaked at 2\ub70%; this rate then remained nearly constant until 1970 and then decreased to 1\ub71% in 2017. Population growth rates in the southeast Asia, east Asia, and Oceania GBD super-region decreased from 2\ub75% in 1963 to 0\ub77% in 2017, whereas in sub-Saharan Africa, population growth rates were almost at the highest reported levels ever in 2017, when they were at 2\ub77%. The global average age increased from 26\ub76 years in 1950 to 32\ub71 years in 2017, and the proportion of the population that is of working age (age 15–64 years) increased from 59\ub79% to 65\ub73%. At the national level, the TFR decreased in all countries and territories between 1950 and 2017; in 2017, TFRs ranged from a low of 1\ub70 livebirths (95% UI 0\ub79–1\ub72) in Cyprus to a high of 7\ub71 livebirths (6\ub78–7\ub74) in Niger. The TFR under age 25 years (TFU25; number of livebirths expected by age 25 years for a hypothetical woman who survived the age group and was exposed to current ASFRs) in 2017 ranged from 0\ub708 livebirths (0\ub707–0\ub709) in South Korea to 2\ub74 livebirths (2\ub72–2\ub76) in Niger, and the TFR over age 30 years (TFO30; number of livebirths expected for a hypothetical woman ageing from 30 to 54 years who survived the age group and was exposed to current ASFRs) ranged from a low of 0\ub73 livebirths (0\ub73–0\ub74) in Puerto Rico to a high of 3\ub71 livebirths (3\ub70–3\ub72) in Niger. TFO30 was higher than TFU25 in 145 countries and territories in 2017. 33 countries had a negative population growth rate from 2010 to 2017, most of which were located in central, eastern, and western Europe, whereas population growth rates of more than 2\ub70% were seen in 33 of 46 countries in sub-Saharan Africa. In 2017, less than 65% of the national population was of working age in 12 of 34 high-income countries, and less than 50% of the national population was of working age in Mali, Chad, and Niger. Interpretation: Population trends create demographic dividends and headwinds (ie, economic benefits and detriments) that affect national economies and determine national planning needs. Although TFRs are decreasing, the global population continues to grow as mortality declines, with diverse patterns at the national level and across age groups. To our knowledge, this is the first study to provide transparent and replicable estimates of population and fertility, which can be used to inform decision making and to monitor progress. Funding: Bill & Melinda Gates Foundation
Feijão guandu cru na alimentação de frangos caipiras criados em sistema semi-intensivo
Search for jet extinction in the inclusive jet-pT spectrum from proton-proton collisions at s=8 TeV
Published by the American Physical Society under the terms of the Creative Commons Attribution 3.0 License. Further distribution of this work must maintain attribution to the author(s) and the published articles title, journal citation, and DOI.The first search at the LHC for the extinction of QCD jet production is presented, using data collected with the CMS detector corresponding to an integrated luminosity of 10.7 fb−1 of proton-proton collisions at a center-of-mass energy of 8 TeV. The extinction model studied in this analysis is motivated by the search for signatures of strong gravity at the TeV scale (terascale gravity) and assumes the existence of string couplings in the strong-coupling limit. In this limit, the string model predicts the suppression of all high-transverse-momentum standard model processes, including jet production, beyond a certain energy scale. To test this prediction, the measured transverse-momentum spectrum is compared to the theoretical prediction of the standard model. No significant deficit of events is found at high transverse momentum. A 95% confidence level lower limit of 3.3 TeV is set on the extinction mass scale
Exposure and dose assessment to particle components among an elderly population
People spend the majority of their time indoors and the composition and toxicity of indoor particles is
very complex and present significant differences comparing with outdoor aerosols. Consequently,
ambient particles cannot represent a real exposure. The aim of this work was to determine the daily
exposure and the daily inhaled dose to particle components of elders living in Elderly Care Centers. A
questionnaire was applied to 193 institutionalized elders in order to achieve their daily time pattern and
to define the micro-environments where PM10 and its components (carbonaceous components and trace
elements) were assessed. Daily exposure was calculated by integrating the elder's time spend in each
micro-environment and the concentration of the pollutants for the period of interest. This parameter,
together with the inhalation rate and the standard body weight, were used to calculate the daily inhaled
dose. PM10 daily exposure and daily inhaled dose ranged between 11 e 16 mg m 3 and 20 10 3 e
28 10 3 mg kg 1, respectively. This work not only allowed a fully quantification of the magnitude of the
elders exposure, but also showed that the assessment of the integrated exposure to PM components is
determinant to accomplish the dose inhaled by elders living in ECCs
Sensibilidade da técnica de imuno-histoquímica em fragmentos de sistema nervoso central de bovinos e equinos naturalmente infectados pelo vírus da raiva
Bioactivity of aqueous extracts of Clibadium sylvestre (Aubl.) Baill. and Derris amazonica Killip on the aphid Myzus persicae (Sulzer, 1776) (Hemiptera: Aphididae)
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