Cyanobacterial diversity in microbial mats from the hypersaline lagoon system of Araruama, Brazil: An in-depth polyphasic study

Abstract

Microbial mats are complex, micro-scale ecosystems that can be found in a wide range of environments. In the top layer of photosynthetic mats from hypersaline environments, a large diversity of cyanobacteria typically predominates. With the aim of strengthening the knowledge on the cyanobacterial diversity present in the coastal lagoon system of Araruama (state of Rio de Janeiro, Brazil), we have characterized three mat samples by means of a polyphasic approach. We have used morphological and molecular data obtained by culture-dependent and -independent methods. Moreover, we have compared different classification methodologies and discussed the outcomes, challenges, and pitfalls of these methods. Overall, we show that Araruama's lagoons harbor a high cyanobacterial diversity. Thirty-six unique morphospecies could be differentiated, which increases by more than 15% the number of morphospecies and genera already reported for the entire Araruama system. Morphology-based data were compared with the 16S rRNA gene phylogeny derived from isolate sequences and environmental sequences obtained by PCR-DGGE and pyrosequencing. Most of the 48 phylotypes could be associated with the observed morphospecies at the order level. More than one third of the sequences demonstrated to be closely affiliated (best BLAST hit results of ≥99%) with cyanobacteria from ecologically similar habitats. Some sequences had no close relatives in the public databases, including one from an isolate, being placed as "loner" sequences within different orders. This hints at hidden cyanobacterial diversity in the mats of the Araruama system, while reinforcing the relevance of using complementary approaches to study cyanobacterial diversity. © 2017 Ramos, Castelo-Branco, Leão, Martins, Carvalhal-Gomes, Sobrinho da Silva, Mendonça Filho and Vasconcelos.This work was supported by the Biogeochemical Project (AMPETRO 14777—Cooperation term 0050.0023165.06.4) of the GSE (Sedimentology Management) Network of PETROBRAS, by the Brazilian National Research Agency— CNPq and by the Research Agency of Rio de Janeiro State—FAPERJ. It was also funded by Portuguese National Funds through FCT—Fundação para a Ciência e a Tecnologia, grants SFRH/BD/80153/2011 to VR, IF/01358/2014 to PL, and UID/Multi/04423/2013 and by the Structured Program of R&D&I INNOVMAR—Innovation and Sustainability in the Management and Exploitation of Marine Resources (reference NORTE-01-0145-FEDER-000035, Research Line NOVELMAR), funded by the Northern Regional Operational Program (NORTE2020) through the European Regional Development Fund (ERDF)