Wnt/β-Catenin Signaling Determines the Vasculogenic Fate of Post-Natal Mesenchymal Stem Cells

Vasculogenesis is the process of de novo blood vessel formation observed primarily during embryonic development. Emerging evidence suggest that post-natal mesenchymal stem cells are capable of recapitulating vasculogenesis when these cells are engaged in tissue regeneration. However, the mechanisms underlining the vasculogenic differentiation of mesenchymal stem cells remain unclear. Here, we used stem cells from human permanent teeth (DPSC) or deciduous teeth (SHED) as models of post-natal primary human mesenchymal stem cells to understand mechanisms regulating their vasculogenic fate. GFP-tagged mesenchymal stem cells seeded in human tooth slice/scaffolds and transplanted into immunodeficient mice differentiate into human blood vessels that anastomize with the mouse vasculature. In vitro, VEGF induced the vasculogenic differentiation of DPSC and SHED via potent activation of Wnt/β-catenin signaling. Further, activation of Wnt signaling is sufficient to induce the vasculogenic differentiation of post-natal mesenchymal stem cells, while Wnt inhibition blocked this process. Notably, β-catenin-silenced DPSC no longer differentiate into endothelial cells in vitro, and showed impaired vasculogenesis in vivo. Collectively, these data demonstrate that VEGF signaling through the canonical Wnt/β-catenin pathway defines the vasculogenic fate of post-natal mesenchymal stem cells

Evaluation of National Health Insurance Program (JKN) Services at Puskesmas PB Selayang II Medan

Evaluation of  Implementation of National Health Insurance Program Services is an assessment or analysis of the implementation of national health insurance program services Based on Law Number 24 of 2011 concerning the Social Security Administering Body (BPJS), the government has taken steps to establish the National.  The purpose of this study was to evaluate the services of the National Health Insurance Program (JKN) at PB Selayang II Health Center using qualitative methods. The research method used in this study is a qualitative research method with descriptive analytic research design. The results of study found that the implementation of the JKN program at the PB Selayang II Health Center had gone well. The Puskesmas has provided adequate facilities and health workers to serve JKN participants and there are still several obstacles in implementing the JKN program at the Puskesmas, namely the use of the Mobile JKN application. The regulation given by the Mayor is in the form of a UHC (Universal Health Coverage) program that guarantees access to health services for all people, regardless of ownership or use of BPJS. With the UHC program, PB Selayang II Health Center is committed to providing fair and equitable health services to all people

Atividade antimicrobiana de espécies de Hypericum

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PELATIHAN BERTANAM SECARA HIDROPONIK SEBAGAI UPAYA PEMBERDAYAAN MASYARAKAT DAN PENGUATAN MODERASI BERAGAMA DI DESA BUNTUT BALI

Pelatihan sistem bertanam secara hidroponik direalisasikan melalui kegiatan KKN (Kuliah Kerja Nyata) Nusantara Moderasi Beragama di desa Buntut Bali. Hal ini sebagai upaya pemberdayaan masyarakat sekaligus upaya penguatan moderasi beragama. Pelatihan ini menggunakan metode Asset-Based Community Development (ABCD). Melalui metode ini, masyarakat menjadi pelaku dan penentu utama dalam upaya pembangunan di lingkungan melalui bertanam secara hidroponik. Tujuannya untuk mengetahui serta memberdayakan setiap potensi dan aset yang dimiliki masyarakat. Pelatihan ini diselenggarakan untuk mengatasi persoalan yang dihadapi oleh masyarakat petani dengan kondisi lokasi lahan pertanian yang jauh. Ilmu pengetahuan yang diperoleh melalui kegiatan ini adalah pengenalan tentang hidroponik sekaligus keterampilan melalui pelatihan bertanam secara hidroponik. Selain itu, pelatihan ini sebagai kegiatan kreatif-kolaboratif antar umat beragama. Hal ini berdampak pada hubungan sosial masyarakat beragama yang hidup berdampingan dan harmonis. Dengan demikian, konteks masyarakat yang beragam dalam bentuk penghayatan kepada Tuhan, terus diperkuat dalam kebersamaan yang saling memberdayakan

Incidence of malignant neoplasms in Porto Alegre and Salvador in 2020 according to DATASUS / Incidência de neoplasias malignas em Porto Alegre e Salvador em 2020 de acordo com o DATASUS

OBJECTIVE: to analyze the incidence of malignant neoplasms in 2020 in two Brazilian cities. METHODS: this is an ecological study with comparative analysis between the populations of the cities of Porto Alegre, and Salvador. Data were extracted from the DATASUS, analyzed in tables and presented in descriptive. RESULTS: The incidence of malignant neoplasms in women aged 30 to 34 years is higher of Porto Alegre than in Salvador, with almost double the number of cases in women compared to men in both cities. In the age 65 to 69, women accounted for 20 more cases in Porto Alegre, and in Salvador, males had 28 more cases. Women underwent more chemotherapy and men more surgical in both cities. CONCLUSION: Differences were observed between the incidence of neoplasms for the cities compared, which could associate determinant variables such as female biological sex with the type of cancer and advanced age. In addition, there is evidence that the southern region of Brazil has a higher incidence than the northeast region, which may be associated with lifestyle habits such as food and culture in the region.OBJETIVO: analizar la incidencia de neoplasias malignas en 2020 en dos ciudades brasileñas. MÉTODOS: se trata de un estudio ecológico con análisis comparativo entre las poblaciones de Porto Alegre y Salvador. Los datos fueron extraídos del DATASUS, analizados en tablas y presentados en gráficos. RESULTADOS: La incidencia de neoplasias malignas en mujeres de 30 a 34 años es mayor en Porto Alegre que en Salvador, con casi el doble de casos en mujeres que en hombres. Entre 65 a 69 años, las mujeres representaron 20 casos más en Porto Alegre, y en Salvador, los hombres tuvieron 28 casos más. Las mujeres se sometieron más a quimioterapia y los hombres más a quirúrgias. CONCLUSIÓN: Se observaron diferencias entre la incidencia de neoplasias, que podrían asociar variables determinantes como el sexo biológico femenino con el tipo de cáncer y la edad avanzada. Existe evidencia de que la región sur de Brasil tiene una mayor incidencia que la región noreste, lo que puede estar asociado con hábitos de estilo de vida como la alimentación y la cultura en la región.Objetivo: analisar a incidência de neoplasias malignas em 2020. Métodos: estudo ecológico com análise comparativa entre as populações de Porto Alegre e Salvador. Foram extraídos dados do DATASUS, analisados em tabelas e apresentados em gráficos. Resultados: a incidência de neoplasias malignas em mulheres entre 30 e 34 anos é maior em Porto Alegre que em Salvador, sendo quase o dobro de casos de mulheres em relação aos homens. Entre 65 e 69 anos, mulheres representaram 20 casos a mais em Porto Alegre, e, em Salvador, o sexo masculino apresentou 28 casos a mais. As mulheres realizaram mais quimioterapias e os homens mais cirurgias. Conclusão: houve diferença entre a incidência de neoplasias nas cidades podendo associar variáveis determinantes como sexo biológico feminino ao tipo de câncer e idade avançada. A maior incidência de casos na região sul pode estar associada aos hábitos de vida como alimentação e cultura desta região.

Análise química de Hypericum carinatum (guttiferae)

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The transcriptional landscape of Shh medulloblastoma

© The Author(s) 2021. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.Sonic hedgehog medulloblastoma encompasses a clinically and molecularly diverse group of cancers of the developing central nervous system. Here, we use unbiased sequencing of the transcriptome across a large cohort of 250 tumors to reveal differences among molecular subtypes of the disease, and demonstrate the previously unappreciated importance of non-coding RNA transcripts. We identify alterations within the cAMP dependent pathway (GNAS, PRKAR1A) which converge on GLI2 activity and show that 18% of tumors have a genetic event that directly targets the abundance and/or stability of MYCN. Furthermore, we discover an extensive network of fusions in focally amplified regions encompassing GLI2, and several loss-of-function fusions in tumor suppressor genes PTCH1, SUFU and NCOR1. Molecular convergence on a subset of genes by nucleotide variants, copy number aberrations, and gene fusions highlight the key roles of specific pathways in the pathogenesis of Sonic hedgehog medulloblastoma and open up opportunities for therapeutic intervention.info:eu-repo/semantics/publishedVersio

Contributions of mean and shape of blood pressure distribution to worldwide trends and variations in raised blood pressure: A pooled analysis of 1018 population-based measurement studies with 88.6 million participants

© The Author(s) 2018. Background: Change in the prevalence of raised blood pressure could be due to both shifts in the entire distribution of blood pressure (representing the combined effects of public health interventions and secular trends) and changes in its high-blood-pressure tail (representing successful clinical interventions to control blood pressure in the hypertensive population). Our aim was to quantify the contributions of these two phenomena to the worldwide trends in the prevalence of raised blood pressure. Methods: We pooled 1018 population-based studies with blood pressure measurements on 88.6 million participants from 1985 to 2016. We first calculated mean systolic blood pressure (SBP), mean diastolic blood pressure (DBP) and prevalence of raised blood pressure by sex and 10-year age group from 20-29 years to 70-79 years in each study, taking into account complex survey design and survey sample weights, where relevant. We used a linear mixed effect model to quantify the association between (probittransformed) prevalence of raised blood pressure and age-group- and sex-specific mean blood pressure. We calculated the contributions of change in mean SBP and DBP, and of change in the prevalence-mean association, to the change in prevalence of raised blood pressure. Results: In 2005-16, at the same level of population mean SBP and DBP, men and women in South Asia and in Central Asia, the Middle East and North Africa would have the highest prevalence of raised blood pressure, and men and women in the highincome Asia Pacific and high-income Western regions would have the lowest. In most region-sex-age groups where the prevalence of raised blood pressure declined, one half or more of the decline was due to the decline in mean blood pressure. Where prevalence of raised blood pressure has increased, the change was entirely driven by increasing mean blood pressure, offset partly by the change in the prevalence-mean association. Conclusions: Change in mean blood pressure is the main driver of the worldwide change in the prevalence of raised blood pressure, but change in the high-blood-pressure tail of the distribution has also contributed to the change in prevalence, especially in older age groups