Phytochemical characterization and radical scavenging activity of aqueous extracts of medicinal plants from Portugal

Aims: The aim of this study was to evaluate the total phenolic and flavonoid contents of four medicinal plants from Portugal usually consumed as tea infusion, Melissa officinalis (Lamiaceae) – lemon balm, Matricaria chamomilla (Asteraceae) – chamomile, Olea europaea (Oleaceae) – olive leaves, and Aloysia triphylla (Verbenaceae) – lemon verbena. Place and Duration of Study: Air-dried leaves of four of the most consumed medicinal plants from Portugal were analyzed in the Department of Pharmacognosy (UFP) and Laboratory of Bromatology (FFUP) and, Department of Pharmacy, and Chemical Laboratory, Health Technology Research Center (CITS) between June 2010 and September 2011. Methodology: Qualitative phytochemical analysis was done for several phytoconstituents (alkaloids, steroids, terpenoids, flavonoids, and tannins). Total phenolic, flavonoid, and tannin contents were quantified by UV-Vis spectrophotometry. It was also analyzed the possible correlation between antioxidant activity (in vitro) and the synergistic effect between different phytochemicals, using the free radical 2,2’-diphenyl-1-picrylhydrazyl (DPPH•). Results: Steroids, terpenoids, flavonoids and tannin, were present in Melissa officinalis and Matricaria chamomilla. In Olea europaea leaves, all the chemical constituents were present except alkaloids, and terpenoids. Aloysia triphylla showed the presence of all the constituents. Total phenol contents ranged from 12.91mg.100g-1 to 87.25 mg.100g-1 and flavonoid contents ranged from 25.17mg.100g-1 to 57.28mg.100g-1. The screening of the leaf of the four selected medicinal plants indicates that the presence of high phenolic content may be due to the presence of tannins and flavonoids which are known to possess antioxidant activities. A slight correlation has been observed between total phenolics and antioxidant activity. Conclusion: Our findings provided evidence that aqueous extracts of these tested plants from Portugal contain medicinally important bioactive compounds. Results showed that plants from Portugal usually used as tea infusions are a good source of phytochemical compounds presenting antioxidant activity, so their consumption must be incremented in younger generations which usually consume other less beneficial drinks.info:eu-repo/semantics/publishedVersio

Nutrition of marine mesograzers: integrating feeding behavior, nutrient intake and performance of an herbivorous amphipod

Consumers can regulate the acquisition and use of nutrients through behavioral and physiological mechanisms. Here, we present an experimental approach that simultaneously integrates multiple nutritional traits, feeding assays, and juvenile performance to assess whether a marine herbivore (the amphipod Ampithoe valida) regulates the intake of elements (carbon and nitrogen), macronutrients (protein and non-protein) or both when offered freeze-dried tissues of seaweeds varying in nutritional content. We assessed behavioral regulation of nutrients in three ways. First, during no-choice assays, we found that amphipods ingested similar amounts of carbon, but not nitrogen, non-protein and protein, across algal diets. Second, herbivore intake rates of carbon, protein and non-protein components across no-choice assays was similar to intake rates when offered a choice of foods. Third, variation in intake rates of carbon and non-protein components among algal diets was significantly greater than was tissue content of these components, while variation in intake rates of nitrogen was significantly lower; differences in protein intake variation was equivocal. While these analytical approaches are not uniformly consistent, carbon and nitrogen seem to emerge as the nutrient components that are more strongly regulated by A. valida. Juveniles reared on single diets shown patterns of survivorship, growth and reproduction that could not be predicted by these feeding preferences, nor nutrient content. We conclude that an integrative approach that considers the intake of multiple nutrients potentially yields insights into feeding behavior and its performance consequences

Enzyme-assisted release of antioxidant peptides from porphyra dioica conchocelis

peer-reviewedThe conchocelis life cycle stage of P. dioica represents an unexplored source of bioactive compounds. The aim of this study was to generate and characterise, for the first time, hydrolysates of conchocelis using a specific combination of proteases (Prolyve® and Flavourzyme®). Hydrolysate molecular mass distribution and free amino acid contents were assessed, and the antioxidant activity was determined using a range of in vitro assays. The protein content and the total amino acid profiles of conchocelis were also studied. Conchocelis contained ~25% of protein (dry weight basis) and had a complete profile of essential amino acids. Direct sequential enzymatic treatment modified the profile of the generated compounds, increasing the amount of low molecular weight peptides (<1 kDa). There was a significant improvement in the antioxidant activity of the hydrolysates compared with the control (up to 2.5-fold), indicating their potential as a novel source of antioxidant ingredients

Modulatory effect of BclI GR gene polymorphisms on the obesity phenotype in Brazilian patients with Cushing's disease

OBJECTIVES: Patients with Cushing's disease exhibit wide phenotypic variability in the severity of obesity, diabetes and hypertension. In the general population, several glucocorticoid receptor genes (NR3C1) and HSD11B1 polymorphisms are associated with altered glucocorticoid sensitivity and/or metabolism, resulting in an increased or reduced risk of an adverse metabolic profile. Our aim was to analyze the association of NR3C1 and HSD11B1 gene variants with the severity of some clinical and hormonal features of Cushing's disease. METHODS: Sixty-four patients presenting with Cushing's disease were diagnosed based on adrenocorticotrophic hormone levels, high-dose dexamethasone suppression tests and/or inferior petrosal sinus sampling and magnetic resonance imaging. The A3669G, ER22/23EK, N363S BclI-NR3C1 and HSD11B1-rs12086634 variants were screened. RESULTS: The BclI, HSD11B1-rs12086634 and A3669G variants were found in 36%, 19.5% and 14% of alleles, respectively. The N363S and ER22/23EK polymorphisms were identified in heterozygosis once in only two patients (1.5% of alleles). There were no differences in the weight gain or prevalence of diabetes and hypertension in the patients carrying the abovementioned alleles compared to the wild-type carriers. Interestingly, the mean body mass index (BMI) of the BclI carriers was significantly higher than the non-carriers (34.4±7 kg/m2 vs. 29.6±4.7 kg/m2, respectively). None of the polymorphisms were associated with the basal adrenocorticotrophic hormone, FU levels or F level after dexamethasone suppression testing. CONCLUSION: Although Cushing's disease results from increased glucocorticoid secretion, we observed that interindividual variability in the peripheral glucocorticoid sensitivity, mediated by the glucocorticoid receptor, could modulate the obesity phenotype

SUPRIMENTO ARTERIAL PARA A BOLSA CLOACAL DE GALINHAS (Gallus gallus Linnaeus, 1758) DA LINHAGEM LABEL ROUGE

Foram estudados a origem, o número e a ordenação dos ramos arteriais destinados à bolsa cloacal em 30 exemplares de Gallus gallus, da linhagem Label Rouge, machos e fêmeas, com idade entre oito e dez semanas. Após injetado o sistema arterial desses animais com solução corada de Neoprene látex, seguido de fixação em solução aquosa de formol a 10 % e ulteriormente dissecados, verificou-se que: a) uma artéria bursocloacal direita esteve presente em 24 dos casos (80,00%), ao passo que a artéria bursocloacal esquerda foi confirmada em 29 dos exemplares (96,66%); b) a bolsa cloacal foi irrigada por um ou dois ramos cloacais, oriundos da artéria bursocloacal ipsilateral; c) a artéria cloacal direita ocorreu em 20 casos (66,66%) e a artéria cloacal esquerda em 9 casos (30,00%), ocasiões em que cediam apenas um ramo; d) o número de ramos arteriais, independentemente de sua origen, variou de dois a quatro, sendo a sua disposição de maneira própria para cada exemplar examinado. Arterial supply for the cloacal bursa of the Rouge Lineage fowl Gallus gallus (Linnaeus, 1758) Abstract A study has been carried out on the origin, number and disposition of the cloacal bursa arterial branches from tirthy male and female fowls (Gallus gallus), of the Label Rouge lineage, ageing between eight and ten weeks. After being injected the arterial system of those animals with a colored solution of Neoprene latex, they were soon after fixed in an 10% aqueous solution of formalin and dissected. The following observations were carried out: a) a right burso cloacal artery was present in 24 of the cases (80.00%), while the left bursocloacal artery was confirmed in 29 of the cases (96.66%); b) the cloacal bursa was irrigated by one or two cloacal branches of the ipsilateral bursocloacal artery; c) the right cloacal artery is present in 20 cases (66.66%) and the left cloacal artery in 9 cases (30.00%), with only one branch; d) the number of arterial branches, independent of origin

The impact of exposure to cartoons promoting healthy eating on children's food preferences and choices

Objective: This study explored whether a cartoon show with healthy eating messages positively affected children's food choices and food preferences.Design: Experimental between-subjects design.Setting: Four elementary schools in Portugal were investigated.Participants: Children (aged 4-8 years; n = 142) were randomly assigned to 1 of 2 groups: a comparison group (n = 73) was exposed to cartoons with no reference to food and an intervention group (n = 69) was exposed to cartoons with healthy eating messages. After viewing, each child was given the opportunity to eat ad libitum for 10 minutes from a small selection of snack foods.Main Outcome Measure: Number of healthy and unhealthy food items chosen. Food preferences were measured using an adapted version of the Leeds Food Preference Checklist.Analysis: Generalized linear models were used to test for differences between groups. Results were considered significant at P <= .05.Results: Children in the experimental group chose significantly more healthy food items than did those in the comparison group (B = -.600; SE = .19; P < .05).Conclusions and Implications: Future studies may address the effect of prolonged exposure to healthy eating cartoons. Cartoons can be used to promote healthy food choices and can be a part of health promotion campaigns.This research was partially supported by Fundacao para a Ciencia e Tecnologia through a European Union COMPETE program grant to Eva Conceicao (IF/01219/2014), and a postdoctoral scholarship (SFRH/BPD/94490/2013) to Ana Rita Vaz, cofinanced by FEDER under the PT2020 Partnership Agreement (UID/PSI/01662/2013) and the project PTDC/MHC-PCL/4974/2012. This research was supported, in part, by the Portuguese Foundation for Science and Technology and the Portuguese Ministry of Science, Technology, and Higher Education through national funds and cofinanced by FEDER through COMPETE2020 under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007653). The authors would like to thank Nutri Ventures for adapting the episodes for the research purposes. They would also like to thank Ana Rito for her suggestions regarding the study protocol

Assessment of penetration of Ascorbyl Tetraisopalmitate into biological membranes by molecular dynamics

The present work, involves the simulation of the transport of a vitamin C derivative, Ascorbyl Tetraisopalmitate (ATI), through human skin by molecular dynamics. Percutaneous absorption of the ATI molecule through the infundibulum, an important route of absorption into the hair follicle of the human skin, has been modeled and compared with the stratum corneum membrane. The comparative study was done, using molecular dynamics with Martini force field. In infundibulum, a single ATI molecule require more time to penetrate, and the data obtained suggested that a high concentration of ATI molecule accelerated the process of penetration. In conclusion, the ATI molecule was found to have more affinity towards the stratum corneum as compared towards the infundibulum and it followed a straight pathway to penetrate (until 600 ns of simulation). In infundibulum, it showed less affinity, more mobility and followed a lateral pathway. Thus, this work contributes to a better understanding of the different molecular interactions during percutaneous absorption of active molecules in these two different types of biological membranes.The authors acknowledge financial support from the Brazilian agencies CAPES, Finep and Fapesp (Project FINEP 01.10.0661-00, FAPESP 2011/13250-0, FAPESP 2013/17247-9, FAPESP 2014/05975-2, CAPES 88887068264/2014-00), of Institute of Research and Development, University of Vale Paraíba

Search for direct production of charginos and neutralinos in events with three leptons and missing transverse momentum in √s = 7 TeV pp collisions with the ATLAS detector

A search for the direct production of charginos and neutralinos in final states with three electrons or muons and missing transverse momentum is presented. The analysis is based on 4.7 fb−1 of proton–proton collision data delivered by the Large Hadron Collider and recorded with the ATLAS detector. Observations are consistent with Standard Model expectations in three signal regions that are either depleted or enriched in Z-boson decays. Upper limits at 95% confidence level are set in R-parity conserving phenomenological minimal supersymmetric models and in simplified models, significantly extending previous results

Search for displaced vertices arising from decays of new heavy particles in 7 TeV pp collisions at ATLAS

We present the results of a search for new, heavy particles that decay at a significant distance from their production point into a final state containing charged hadrons in association with a high-momentum muon. The search is conducted in a pp-collision data sample with a center-of-mass energy of 7 TeV and an integrated luminosity of 33 pb^-1 collected in 2010 by the ATLAS detector operating at the Large Hadron Collider. Production of such particles is expected in various scenarios of physics beyond the standard model. We observe no signal and place limits on the production cross-section of supersymmetric particles in an R-parity-violating scenario as a function of the neutralino lifetime. Limits are presented for different squark and neutralino masses, enabling extension of the limits to a variety of other models.Comment: 8 pages plus author list (20 pages total), 8 figures, 1 table, final version to appear in Physics Letters

Jet size dependence of single jet suppression in lead-lead collisions at sqrt(s(NN)) = 2.76 TeV with the ATLAS detector at the LHC

Measurements of inclusive jet suppression in heavy ion collisions at the LHC provide direct sensitivity to the physics of jet quenching. In a sample of lead-lead collisions at sqrt(s) = 2.76 TeV corresponding to an integrated luminosity of approximately 7 inverse microbarns, ATLAS has measured jets with a calorimeter over the pseudorapidity interval |eta| < 2.1 and over the transverse momentum range 38 < pT < 210 GeV. Jets were reconstructed using the anti-kt algorithm with values for the distance parameter that determines the nominal jet radius of R = 0.2, 0.3, 0.4 and 0.5. The centrality dependence of the jet yield is characterized by the jet "central-to-peripheral ratio," Rcp. Jet production is found to be suppressed by approximately a factor of two in the 10% most central collisions relative to peripheral collisions. Rcp varies smoothly with centrality as characterized by the number of participating nucleons. The observed suppression is only weakly dependent on jet radius and transverse momentum. These results provide the first direct measurement of inclusive jet suppression in heavy ion collisions and complement previous measurements of dijet transverse energy imbalance at the LHC.Comment: 15 pages plus author list (30 pages total), 8 figures, 2 tables, submitted to Physics Letters B. All figures including auxiliary figures are available at http://atlas.web.cern.ch/Atlas/GROUPS/PHYSICS/PAPERS/HION-2011-02