Architecture, the City, and Nature: Part and Whole?

Despite all the differences that immediately come to mind when natural and artificial landscapes are compared, we tend to believe that they are or should be similarly whole and entire, that each is best when characterized by unity. The same is true for individual buildings: each should be all-of-a-piece

The Beginning of the Beginning: Kahn and Architectural Education in Philadelphia

Paul Philippe Cret was one of Penn’s greatest teachers and one of the city’s greatest architects. Louis I. Kahn, the University’s most well–known teacher, was one of Cret’s students. Holmes Perkins, educated at Harvard under Walter Gropius, reshaped the School and changed its orientation. The key task of the three architects was to articulate a new understanding of what is specific to the discipline, recreating its professional and intellectual center and orientation. This would not require the replacement or elimination of what had been developed in the preceding years; instead the task was to augment it with a more focused sense of what architecture itself is all about

Architectures of the Text: An Inquiry Into the Hypnerotomachia Poliphili

Architectures of the Text: An Inquiry Into the Hypnerotomachia Poliphili A symposium to celebrate the acquisition of the second edition of the Hypnerotomachia Poliphili (1545) by the University of Pennsylvania Libraries. Website To download podcasts of the lectures, select the additional files below. Files in .mp4 format include images; files in .mp3 format are audio only. To download the symposium program, select download button at right. In April 2011, the University of Pennsylvania Libraries acquired a copy of the uncommon second edition of Francesco Colonna’s Hypnerotomachia Poliphili (Venice 1545). Since the appearance of the first edition in 1499, the Hypnerotomachia Poliphili has been heralded as the most beautiful book to appear in the Italian Renaissance. Printed in Venice by Aldus Manutius, “The Dream of Poliphily” was admired by Aldus’s contemporaries for its scholarship and value as an architectural treatise. Forty-six years after the publication of the first edition, Aldus’s heirs printed a second edition in 1545. This second edition suggests a renewed interest in the work, within Italy and beyond, for within a year a French translation appeared, followed by an English translation in 1592. Celebrated for its typographical design and illustrations, the Hypnerotomachia continues to attract the interest of scholars, typophiles, and collectors; it remains available in modern scholarly editions in both print and electronic format. The University of Pennsylvania Libraries\u27 acquisition came at the suggestion of John Dixon Hunt, Professor Emeritus of Landscape Architecture at the University. Funds for its purchase came from the G. Holmes Perkins Books and Archives Fund, established by G. Holmes Perkins, Professor of Architecture and Urbanism and former dean of the Graduate School of Fine Arts (now the School of Design). The Libraries and the School of Design administer this fund jointly. On February 11, 2012, the Anne and Jerome Fisher Fine Arts Library, the Rare Book and Manuscript Library, and the School of Design collaborated on a one-day symposium to celebrate the acquisition of the Hypnerotomachia. Presentations took place in the Class of \u2755 room, Van Pelt-Dietrich Library. Program: 10:30am-11:30am Movement 1: Books and Histories Welcome: David McKnight William B. Keller, Hypnerotomachia Joins the Perkins Library: Collecting to Support Persuasion in Architectural Design and History Eric Pumroy, Remarks on the 1499 Hypnerotomachia Poliphili at Bryn Mawr Special Collections John Dixon Hunt, Hypnerotomachia Poliphili: A Child\u27s Guide to the Story Line and a Look at its Afterlives Lynne Farrington, \u27Though I could lead a quiet and peaceful life, I have chosen one full of toil and trouble\u27: Aldus Manutius and the Printing History of the Hypnerotomachia Poliphili 11:30am-1:00pm Movement 2: Words and Interpretations Victoria Kirkham, Hypno What? A Dreamer\u27s Vision and the Reader\u27s Nightmare Ann Moyer, The Wanderings of Poliphilo through Renaissance Studies Ian White, Multiple Words, Multiple Meanings in the Hypnerotomachia 2:00pm-3:00pm Movement 3: Art and Illustration Chris Nygren, The Hypnerotomachia and Italian Art Circa 1500 Larry Silver, Not Hypnerotomachia: Venice\u27s Other Early Woodcut Illustrations 3:00pm-4:30pm Movement 4: Imagined Architectures Raffaella Fabiani Giannetto, \u27Not before either known or dreamt of\u27: The Hypnerotomachia Poliphili and the Craft of Wonder David Leatherbarrow, What Fragments are to Desire, Elements are to Design Ian White, Mathematical Design in Poliphilo\u27s Imaginary Building, The Temple of Venus 4:30pm-5:00pm Break and Interlude Shushi Yoshinaga, Hypnerotomachia Poliphili: A Modern Heritage : a display of objects and images 5:00pm-6:00pm Movement 5: Contemporary Resonances and Final Observation

Structural Basis for Specificity of Propeptide-Enzyme Interaction in Barley C1A Cysteine Peptidases

C1A cysteine peptidases are synthesized as inactive proenzymes. Activation takes place by proteolysis cleaving off the inhibitory propeptide. The inhibitory capacity of propeptides from barley cathepsin L and B-like peptidases towards commercial and barley cathepsins has been characterized. Differences in selectivity have been found for propeptides from L-cathepsins against their cognate and non cognate enzymes. Besides, the propeptide from barley cathepsin B was not able to inhibit bovine cathepsin B. Modelling of their three-dimensional structures suggests that most propeptide inhibitory properties can be explained from the interaction between the propeptide and the mature cathepsin structures. Their potential use as biotechnological tools is discussed

Validation of N-myristoyltransferase as an antimalarial drug target using an integrated chemical biology approach

Malaria is an infectious disease caused by parasites of the genus Plasmodium, which leads to approximately one million deaths per annum worldwide. Chemical validation of new antimalarial targets is urgently required in view of rising resistance to current drugs. One such putative target is the enzyme N-myristoyltransferase, which catalyses the attachment of the fatty acid myristate to protein substrates (N-myristoylation). Here, we report an integrated chemical biology approach to explore protein myristoylation in the major human parasite P. falciparum, combining chemical proteomic tools for identification of the myristoylated and glycosylphosphatidylinositol-anchored proteome with selective small-molecule N-myristoyltransferase inhibitors. We demonstrate that N-myristoyltransferase is an essential and chemically tractable target in malaria parasites both in vitro and in vivo, and show that selective inhibition of N-myristoylation leads to catastrophic and irreversible failure to assemble the inner membrane complex, a critical subcellular organelle in the parasite life cycle. Our studies provide the basis for the development of new antimalarials targeting N-myristoyltransferase