From the web of data to a world of action

This is the author’s version of a work that was accepted for publication in Web Semantics: Science, Services and Agents on the World Wide Web. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Web Semantics: Science, Services and Agents on the World Wide Web 8.4 (2010): 10.1016/j.websem.2010.04.007This paper takes as its premise that the web is a place of action, not just information, and that the purpose of global data is to serve human needs. The paper presents several component technologies, which together work towards a vision where many small micro-applications can be threaded together using automated assistance to enable a unified and rich interaction. These technologies include data detector technology to enable any text to become a start point of semantic interaction; annotations for web-based services so that they can link data to potential actions; spreading activation over personal ontologies, to allow modelling of context; algorithms for automatically inferring 'typing' of web-form input data based on previous user inputs; and early work on inferring task structures from action traces. Some of these have already been integrated within an experimental web-based (extended) bookmarking tool, Snip!t, and a prototype desktop application On Time, and the paper discusses how the components could be more fully, yet more openly, linked in terms of both architecture and interaction. As well as contributing to the goal of an action and activity-focused web, the work also exposes a number of broader issues, theoretical, practical, social and economic, for the Semantic Web.Parts of this work were supported by the Information Society Technologies (IST) Program of the European Commission as part of the DELOS Network of Excellence on Digital Libraries (Contract G038- 507618). Thanks also to Emanuele Tracanna, Marco Piva, and Raffaele Giuliano for their work on On Time

Distinct roles of Arabidopsis ORC1 proteins in DNA replication and heterochromatic H3K27me1 deposition

13 Pág.Most cellular proteins involved in genome replication are conserved in all eukaryotic lineages including yeast, plants and animals. However, the mechanisms controlling their availability during the cell cycle are less well defined. Here we show that the Arabidopsis genome encodes for two ORC1 proteins highly similar in amino acid sequence and that have partially overlapping expression domains but with distinct functions. The ancestral ORC1b gene, present before the partial duplication of the Arabidopsis genome, has retained the canonical function in DNA replication. ORC1b is expressed in both proliferating and endoreplicating cells, accumulates during G1 and is rapidly degraded upon S-phase entry through the ubiquitin-proteasome pathway. In contrast, the duplicated ORC1a gene has acquired a specialized function in heterochromatin biology. ORC1a is required for efficient deposition of the heterochromatic H3K27me1 mark by the ATXR5/6 histone methyltransferases. The distinct roles of the two ORC1 proteins may be a feature common to other organisms with duplicated ORC1 genes and a major difference with animal cells.This research was supported by grants BFU2015-68396-R, RTI2018-094793-B-I00 (Ministerio de Ciencia e Innovacion) and AdG_833617 (European Research Council) to C.G., grant LABEX: ANR-10-LABX-0036-NETRNA and ANR-19-CE13-0032-01 to P.G. and S.N., and by institutional grants from Banco de Santander and Fundación Ramón Areces to the CBMSO.Peer reviewe

A step towards mobile arsenic measurement for surface waters.

Surface modified quantum dots (QDs) are studied using a bio-inspired cysteine rich ligand (glutathione, GSH) and their quenching response and selectivity to arsenic examined. As predicted from As(3+) binding with highly crosslinked phytochelatin-(PCn)-like molecules, better arsenic selectivity is obtained for a thicker more 3-dimensional GSH surface layer, with exposed sulfhydryl groups. A detection limit of at least 10 μM can be achieved using CdSe/ZnS core-shell QDs capped with this GSH structure. The system is also demonstrated using a mobile phone camera to record the measurement, producing a detection limit of 5 μM. However, copper remains the main interferent of concern. Water-soluble CdTe QDs show little sensitivity to As(3+) even with a GSH surface, but they remain sensitive to Cu(2+), allowing a copper baseline to be established from the CdTe measurement. Despite anticipating that spectrally non overlapping fluorescence would be required from the two types of QDs to achieve this, a method is demonstrated using RGB channels from a mobile phone and processing the raw data for CdTe QDs, with an emission wavelength of 600 nm, and CdSe/ZnS QDs, with emission maximum of 630 nm. It is shown that As(3+) measurement remains feasible at the WHO guideline value of 10 μg L(-1) up to a copper concentration of around 0.3 μM Cu(2+), which corresponds to the highest recorded level in a selection of large rivers world-wide.This is the author accepted manuscript. The final version is available via RSC at http://pubs.rsc.org/en/Content/ArticleLanding/2015/AN/c4an02368d#!divAbstract

A metastable equilibrium model for the relative abundances of microbial phyla in a hot spring

Many studies link the compositions of microbial communities to their environments, but the energetics of organism-specific biomass synthesis as a function of geochemical variables has rarely been assessed. We describe a thermodynamic model that integrates geochemical and metagenomic data for biofilms sampled at five sites along a thermal and chemical gradient in the outflow channel of the hot spring known as ‘‘Bison Pool’’ in Yellowstone National Park. The relative abundances of major phyla in individual communities sampled along the outflow channel are modeled by computing metastable equilibrium among model proteins with amino acid compositions derived from metagenomic sequences. Geochemical conditions are represented by temperature and activities of basis species, including pH and oxidation-reduction potential quantified as the activity of dissolved hydrogen. By adjusting the activity of hydrogen, the model can be tuned to closely approximate the relative abundances of the phyla observed in the community profiles generated from BLAST assignments. The findings reveal an inverse relationship between the energy demand to form the proteins at equal thermodynamic activities and the abundance of phyla in the community.Although the metabolisms used by many members of these communities are driven by chemical disequilibria, the results support the possibility that higher-level patterns of chemotrophic microbial ecosystems are shaped by metastable equilibrium states that depend on both the composition of biomass and the environmental conditions

Antimicrobial resistance among migrants in Europe: a systematic review and meta-analysis

BACKGROUND: Rates of antimicrobial resistance (AMR) are rising globally and there is concern that increased migration is contributing to the burden of antibiotic resistance in Europe. However, the effect of migration on the burden of AMR in Europe has not yet been comprehensively examined. Therefore, we did a systematic review and meta-analysis to identify and synthesise data for AMR carriage or infection in migrants to Europe to examine differences in patterns of AMR across migrant groups and in different settings. METHODS: For this systematic review and meta-analysis, we searched MEDLINE, Embase, PubMed, and Scopus with no language restrictions from Jan 1, 2000, to Jan 18, 2017, for primary data from observational studies reporting antibacterial resistance in common bacterial pathogens among migrants to 21 European Union-15 and European Economic Area countries. To be eligible for inclusion, studies had to report data on carriage or infection with laboratory-confirmed antibiotic-resistant organisms in migrant populations. We extracted data from eligible studies and assessed quality using piloted, standardised forms. We did not examine drug resistance in tuberculosis and excluded articles solely reporting on this parameter. We also excluded articles in which migrant status was determined by ethnicity, country of birth of participants' parents, or was not defined, and articles in which data were not disaggregated by migrant status. Outcomes were carriage of or infection with antibiotic-resistant organisms. We used random-effects models to calculate the pooled prevalence of each outcome. The study protocol is registered with PROSPERO, number CRD42016043681. FINDINGS: We identified 2274 articles, of which 23 observational studies reporting on antibiotic resistance in 2319 migrants were included. The pooled prevalence of any AMR carriage or AMR infection in migrants was 25·4% (95% CI 19·1-31·8; I2 =98%), including meticillin-resistant Staphylococcus aureus (7·8%, 4·8-10·7; I2 =92%) and antibiotic-resistant Gram-negative bacteria (27·2%, 17·6-36·8; I2 =94%). The pooled prevalence of any AMR carriage or infection was higher in refugees and asylum seekers (33·0%, 18·3-47·6; I2 =98%) than in other migrant groups (6·6%, 1·8-11·3; I2 =92%). The pooled prevalence of antibiotic-resistant organisms was slightly higher in high-migrant community settings (33·1%, 11·1-55·1; I2 =96%) than in migrants in hospitals (24·3%, 16·1-32·6; I2 =98%). We did not find evidence of high rates of transmission of AMR from migrant to host populations. INTERPRETATION: Migrants are exposed to conditions favouring the emergence of drug resistance during transit and in host countries in Europe. Increased antibiotic resistance among refugees and asylum seekers and in high-migrant community settings (such as refugee camps and detention facilities) highlights the need for improved living conditions, access to health care, and initiatives to facilitate detection of and appropriate high-quality treatment for antibiotic-resistant infections during transit and in host countries. Protocols for the prevention and control of infection and for antibiotic surveillance need to be integrated in all aspects of health care, which should be accessible for all migrant groups, and should target determinants of AMR before, during, and after migration. FUNDING: UK National Institute for Health Research Imperial Biomedical Research Centre, Imperial College Healthcare Charity, the Wellcome Trust, and UK National Institute for Health Research Health Protection Research Unit in Healthcare-associated Infections and Antimictobial Resistance at Imperial College London

Mobility across the pre-Pyrenean mountain ranges during the Chalcolithic through strontium isotopes in human enamel: La Cueva de los Cristales (Sarsa de Surta, Huesca, Spain)

There is an increasing abundance in the archaeological record in Iberia for the Late Neolithic and the beginning of the Chalcolithic periods, mostly regarding burials. The higher pre-Pyrenean areas began to be settled more frequently, but the poor weather conditions have led researchers to suggest that human presence mostly took the form of sporadic visits. This argument has provoked substantial controversy given the increase not only in the archaeological artefacts recorded but also in the number of burial sites in less accessible places. To shed more light on the knowledge of these Chalcolithic mountain groups, we have carried out strontium isotope analysis of human enamel of individuals from the funerary cave Cueva de los Cristales (Sarsa de Surta, Huesca, España), located 1,300 m above sea level (m a.s.l.). Our results point to a pre-Pyrenean origin of the Chalcolithic groups, despite differences found among the individual values, which may be related to different locations in the pre-Pyrenean area. The added value of the study resides in the large amount of data points (up to 40) of bioavailable strontium values which will be useful for future mobility studies performed in the Pyrenean territory

Overlapping political budget cycles in the legislative and the executive

We advance the literature on political budget cycles by testing separately for cycles in expenditures for elections in the legislative and the executive. Using municipal data, we can separately identify these cycles and account for general year effects. For the executive branch, we show that it is important whether the incumbent re-runs. To account for the potential endogeneity associated with this decision, we apply a unique instrumental variables approach based on age and pension eligibility rules. We find sizable and significant effects in expenditures before council elections and before joint elections when the incumbent re-runs