Solar ultraviolet radiation is necessary to enhance grapevine fruit ripening transcriptional and phenolic responses

Background: Ultraviolet (UV) radiation modulates secondary metabolism in the skin of Vitis vinifera L. berries, which affects the final composition of both grapes and wines. The expression of several phenylpropanoid biosynthesis-related genes is regulated by UV radiation in grape berries. However, the complete portion of transcriptome and ripening processes influenced by solar UV radiation in grapes remains unknown.Results: Whole genome arrays were used to identify the berry skin transcriptome modulated by the UV radiation received naturally in a mid-altitude Tempranillo vineyard. UV radiation-blocking and transmitting filters were used to generate the experimental conditions. The expression of 121 genes was significantly altered by solar UV radiation. Functional enrichment analysis of altered transcripts mainly pointed out that secondary metabolism-related transcripts were induced by UV radiation including VvFLS1, VvGT5 and VvGT6 flavonol biosynthetic genes and monoterpenoid biosynthetic genes. Berry skin phenolic composition was also analysed to search for correlation with gene expression changes and UV-increased flavonols accumulation was the most evident impact. Among regulatory genes, novel UV radiation-responsive transcription factors including VvMYB24 and three bHLH, together with known grapevine UV-responsive genes such as VvMYBF1, were identified. A transcriptomic meta-analysis revealed that genes up-regulated by UV radiation in the berry skin were also enriched in homologs of Arabidopsis UVR8 UV-B photoreceptor-dependent UV-B -responsive genes. Indeed, a search of the grapevine reference genomic sequence identified UV-B signalling pathway homologs and among them, VvHY5-1, VvHY5-2 and VvRUP were up-regulated by UV radiation in the berry skin.Conclusions: Results suggest that the UV-B radiation-specific signalling pathway is activated in the skin of grapes grown at mid-altitudes. The biosynthesis and accumulation of secondary metabolites, which are appreciated in winemaking and potentially confer cross-tolerance, were almost specifically triggered. This draws attention to viticultural practices that increase solar UV radiation on vineyards as they may improve grape features. © 2014 Carbonell-Bejerano et al.; licensee BioMed Central Ltd

Solar ultraviolet radiation is necessary to enhance grapevine fruit ripening transcriptional and phenolic responses

Background: Ultraviolet (UV) radiation modulates secondary metabolism in the skin of Vitis vinifera L. berries, which affects the final composition of both grapes and wines. The expression of several phenylpropanoid biosynthesis-related genes is regulated by UV radiation in grape berries. However, the complete portion of transcriptome and ripening processes influenced by solar UV radiation in grapes remains unknown.Results: Whole genome arrays were used to identify the berry skin transcriptome modulated by the UV radiation received naturally in a mid-altitude Tempranillo vineyard. UV radiation-blocking and transmitting filters were used to generate the experimental conditions. The expression of 121 genes was significantly altered by solar UV radiation. Functional enrichment analysis of altered transcripts mainly pointed out that secondary metabolism-related transcripts were induced by UV radiation including VvFLS1, VvGT5 and VvGT6 flavonol biosynthetic genes and monoterpenoid biosynthetic genes. Berry skin phenolic composition was also analysed to search for correlation with gene expression changes and UV-increased flavonols accumulation was the most evident impact. Among regulatory genes, novel UV radiation-responsive transcription factors including VvMYB24 and three bHLH, together with known grapevine UV-responsive genes such as VvMYBF1, were identified. A transcriptomic meta-analysis revealed that genes up-regulated by UV radiation in the berry skin were also enriched in homologs of Arabidopsis UVR8 UV-B photoreceptor-dependent UV-B -responsive genes. Indeed, a search of the grapevine reference genomic sequence identified UV-B signalling pathway homologs and among them, VvHY5-1, VvHY5-2 and VvRUP were up-regulated by UV radiation in the berry skin.Conclusions: Results suggest that the UV-B radiation-specific signalling pathway is activated in the skin of grapes grown at mid-altitudes. The biosynthesis and accumulation of secondary metabolites, which are appreciated in winemaking and potentially confer cross-tolerance, were almost specifically triggered. This draws attention to viticultural practices that increase solar UV radiation on vineyards as they may improve grape features. © 2014 Carbonell-Bejerano et al.; licensee BioMed Central Ltd.ENO and JMA are grateful to the Ministerio de Economía y Competitividad of Spain and the Fondo Europeo de Desarrollo Regional (FEDER) for financial support (Project CGL2011-26937). This study was funded in part by Project BIO2011-026229 from the Spanish MINECO. Microarray hybridizations were carried out at the Genomics Unit of the National Centre for Biotechnology, CNB-CSIC, Madrid, Spain. The present work is integrated in the COST (European Cooperation in Science and Technology) Action FA0906 of the European Union “UV-B radiation: a specific regulator of plant growth and food quality in a changing climate” as well as COST Action FA1106 “Quality fruit”.Peer Reviewe

Challenges of viticulture adaptation to global change: tackling the issue from the roots

Viticulture is facing emerging challenges not only because of the effect of climate change on yield and composition of grapes, but also of a social demand for environmental‐friendly agricultural management. Adaptation to these challenges is essential to guarantee the sustainability of viticulture. The aim of this review is to present adaptation possibilities from the soil‐hidden, and often disregarded, part of the grapevine, the roots. The complexity of soil–root interactions makes necessary a comprehensive approach taking into account physiology, pathology and genetics, in order to outline strategies to improve viticulture adaptation to current and future threats. Rootstocks are the link between soil and scion in grafted crops, and they have played an essential role in viticulture since the introduction of phylloxera into Europe at the end of the 19th century. This review outlines current and future challenges that are threatening the sustainability of the wine sector and the relevant role that rootstocks can play to face these threats. We describe how rootstocks along with soil management can be exploited as an essential tool to deal with the effects of climate change and of emerging soil‐borne pests and pathogens. Moreover, we discuss the possibilities and limitations of diverse genetic strategies for rootstock breeding.info:eu-repo/semantics/publishedVersio

Challenges of viticulture adaptation to global change: tackling the issue from the roots

[EN] Viticulture is facing emerging challenges not only because of the effect of climate change on yield and composition of grapes, but also of a social demand for environmental-friendly agricultural management. Adaptation to these challenges is essential to guarantee the sustainability of viticulture. The aim of this review is to present adaptation possibilities from the soil-hidden, and often disregarded, part of the grapevine, the roots. The complexity of soil-root interactions makes necessary a comprehensive approach taking into account physiology, pathology and genetics, in order to outline strategies to improve viticulture adaptation to current and future threats. Rootstocks are the link between soil and scion in grafted crops, and they have played an essential role in viticulture since the introduction of phylloxera into Europe at the end of the 19th century. This review outlines current and future challenges that are threatening the sustainability of the wine sector and the relevant role that rootstocks can play to face these threats. We describe how rootstocks along with soil management can be exploited as an essential tool to deal with the effects of climate change and of emerging soil-borne pests and pathogens. Moreover, we discuss the possibilities and limitations of diverse genetic strategies for rootstock breeding.This work is framed in the networking activities of RedVitis (AGL2015-70931-REDT) and RedVitis 2.0 (AGL2017-90759-REDT), funded by the State Research Agency (AEI) of the Spanish Ministry of Science and Innovation. Ms Diana Marin is beneficiary of postgraduate scholarship funded by Universidad Publica de Navarra (FPI-UPNA-2016). Dr Juan Emilio Palomares-Rius acknowledges the State Research Agency (AEI) of the Spanish Ministry of Science and Innovation for the 'Ramon y Cajal' Fellowship RYC-2017-22228 and Dr David Gramaje acknowledges Spanish Ministry of Economy and Competitiveness for the 'Ramon y Cajal' Fellowship RYC-2017-23098.Marín, D.; Armengol Fortí, J.; Carbonell-Bejerano, P.; Escalona, J.; Gramaje Pérez, D.; Hernández-Montes, E.; Intrigliolo, DS.... (2021). Challenges of viticulture adaptation to global change: tackling the issue from the roots. Australian Journal of Grape and Wine Research. 27(1):8-25. https://doi.org/10.1111/ajgw.12463S825271AGÜERO, C. B., URATSU, S. L., GREVE, C., POWELL, A. L. T., LABAVITCH, J. M., MEREDITH, C. P., & DANDEKAR, A. M. (2005). Evaluation of tolerance to Pierce’s disease andBotrytisin transgenic plants ofVitis viniferaL. expressing the pear PGIP gene. Molecular Plant Pathology, 6(1), 43-51. doi:10.1111/j.1364-3703.2004.00262.xAgustí-Brisach, C., Mostert, L., & Armengol, J. (2013). Detection and quantification ofIlyonectriaspp. associated with black-foot disease of grapevine in nursery soils using multiplex nested PCR and quantitative PCR. Plant Pathology, 63(2), 316-322. doi:10.1111/ppa.12093Agustí-Brisach, C., Gramaje, D., García-Jiménez, J., & Armengol, J. (2013). Detection of black-foot disease pathogens in the grapevine nursery propagation process in Spain. European Journal of Plant Pathology, 137(1), 103-112. doi:10.1007/s10658-013-0221-8Alaniz, S., García-Jiménez, J., Abad-Campos, P., & Armengol, J. (2010). Susceptibility of grapevine rootstocks to Cylindrocarpon liriodendri and C. macrodidymum. Scientia Horticulturae, 125(3), 305-308. doi:10.1016/j.scienta.2010.04.009Alaniz, S., Armengol, J., León, M., García-Jiménez, J., & Abad-Campos, P. (2009). Analysis of genetic and virulence diversity of Cylindrocarpon liriodendri and C. macrodidymum associated with black foot disease of grapevine. Mycological Research, 113(1), 16-23. doi:10.1016/j.mycres.2008.07.002Albacete, A., Martinez-Andujar, C., Martinez-Perez, A., Thompson, A. J., Dodd, I. C., & Perez-Alfocea, F. (2015). Unravelling rootstockxscion interactions to improve food security. Journal of Experimental Botany, 66(8), 2211-2226. doi:10.1093/jxb/erv027Aragüés, R., Medina, E. T., Zribi, W., Clavería, I., Álvaro-Fuentes, J., & Faci, J. (2014). Soil salinization as a threat to the sustainability of deficit irrigation under present and expected climate change scenarios. Irrigation Science, 33(1), 67-79. doi:10.1007/s00271-014-0449-xBarrios-Masias, F. H., Knipfer, T., Walker, M. A., & McElrone, A. J. (2019). Differences in hydraulic traits of grapevine rootstocks are not conferred to a common Vitis vinifera scion. Functional Plant Biology, 46(3), 228. doi:10.1071/fp18110Bavaresco, L., Gardiman, M., Brancadoro, L., Espen, L., Failla, O., Scienza, A., … Testolin, R. (2015). Grapevine breeding programs in Italy. Grapevine Breeding Programs for the Wine Industry, 135-157. doi:10.1016/b978-1-78242-075-0.00007-7Berdeja, M., Nicolas, P., Kappel, C., Dai, Z. W., Hilbert, G., Peccoux, A., … Delrot, S. (2015). Water limitation and rootstock genotype interact to alter grape berry metabolism through transcriptome reprogramming. Horticulture Research, 2(1). doi:10.1038/hortres.2015.12Bert, P.-F., Bordenave, L., Donnart, M., Hévin, C., Ollat, N., & Decroocq, S. (2012). Mapping genetic loci for tolerance to lime-induced iron deficiency chlorosis in grapevine rootstocks (Vitis sp.). Theoretical and Applied Genetics, 126(2), 451-473. doi:10.1007/s00122-012-1993-5Bianchi, D., Grossi, D., Tincani, D. T. G., Simone Di Lorenzo, G., Brancadoro, L., & Rustioni, L. (2018). Multi-parameter characterization of water stress tolerance in Vitis hybrids for new rootstock selection. Plant Physiology and Biochemistry, 132, 333-340. doi:10.1016/j.plaphy.2018.09.018Bonada, M., Jeffery, D. W., Petrie, P. R., Moran, M. A., & Sadras, V. O. (2015). Impact of elevated temperature and water deficit on the chemical and sensory profiles of Barossa Shiraz grapes and wines. Australian Journal of Grape and Wine Research, 21(2), 240-253. doi:10.1111/ajgw.12142Borie, B., Jacquiot, L., Jamaux-Despréaux, I., Larignon, P., & Péros, J.-P. (2002). Genetic diversity in populations of the fungiPhaeomoniella chlamydosporaandPhaeoacremonium aleophilumon grapevine in France. Plant Pathology, 51(1), 85-96. doi:10.1046/j.0032-0862.2001.658.xBravdo, B. (2012). EFFECTS OF SALINITY AND IRRIGATION WITH DESALINATED EFFLUENT AND SEA WATER ON PRODUCTION AND FRUIT QUALITY OF GRAPEVINES (REVIEW AND UPDATE). Acta Horticulturae, (931), 245-258. doi:10.17660/actahortic.2012.931.27Brown, D. S., Jaspers, M. V., Ridgway, H. J., Barclay, C. J., & Jones, E. E. (2013). Susceptibility of four grapevine rootstocks to Cylindrocladiella parva. New Zealand Plant Protection, 66, 249-253. doi:10.30843/nzpp.2013.66.5675Brunori, E., Farina, R., & Biasi, R. (2016). Sustainable viticulture: The carbon-sink function of the vineyard agro-ecosystem. Agriculture, Ecosystems & Environment, 223, 10-21. doi:10.1016/j.agee.2016.02.012Cabral, A., Rego, C., Nascimento, T., Oliveira, H., Groenewald, J. Z., & Crous, P. W. (2012). Multi-gene analysis and morphology reveal novel Ilyonectria species associated with black foot disease of grapevines. Fungal Biology, 116(1), 62-80. doi:10.1016/j.funbio.2011.09.010Carbonell-Bejerano, P., Santa María, E., Torres-Pérez, R., Royo, C., Lijavetzky, D., Bravo, G., … Martínez-Zapater, J. M. (2013). Thermotolerance Responses in Ripening Berries of Vitis vinifera L. cv Muscat Hamburg. Plant and Cell Physiology, 54(7), 1200-1216. doi:10.1093/pcp/pct071Carneiro, R., Randig, O., Almeida, M. R., & Gomes, A. C. (2004). Additional information on Meloidogyne ethiopica Whitehead, 1968 (Tylenchida: Meloidogynidae), a root-knot nematode parasitising kiwi fruit and grape-vine from Brazil and Chile. Nematology, 6(1), 109-123. doi:10.1163/156854104323072982Castellarin, S. D., Matthews, M. A., Di Gaspero, G., & Gambetta, G. A. (2007). Water deficits accelerate ripening and induce changes in gene expression regulating flavonoid biosynthesis in grape berries. Planta, 227(1), 101-112. doi:10.1007/s00425-007-0598-8Chaverri, P., Salgado, C., Hirooka, Y., Rossman, A. Y., & Samuels, G. J. (2011). Delimitation of Neonectria and Cylindrocarpon (Nectriaceae, Hypocreales, Ascomycota) and related genera with Cylindrocarpon-like anamorphs. Studies in Mycology, 68, 57-78. doi:10.3114/sim.2011.68.03Chaves, M. M., Zarrouk, O., Francisco, R., Costa, J. M., Santos, T., Regalado, A. P., … Lopes, C. M. (2010). Grapevine under deficit irrigation: hints from physiological and molecular data. Annals of Botany, 105(5), 661-676. doi:10.1093/aob/mcq030Chitarra, W., Perrone, I., Avanzato, C. G., Minio, A., Boccacci, P., Santini, D., … Gambino, G. (2017). Grapevine Grafting: Scion Transcript Profiling and Defense-Related Metabolites Induced by Rootstocks. Frontiers in Plant Science, 8. doi:10.3389/fpls.2017.00654Clark, J. R., & Finn, C. E. (2010). Register of New Fruit and Nut Cultivars List 45. HortScience, 45(5), 716-756. doi:10.21273/hortsci.45.5.716Clingeleffer, P., Morales, N., Davis, H., & Smith, H. (2019). The significance of scion × rootstock interactions. OENO One, 53(2). doi:10.20870/oeno-one.2019.53.2.2438COMAS, L. H., BAUERLE, T. L., & EISSENSTAT, D. M. (2010). Biological and environmental factors controlling root dynamics and function: effects of root ageing and soil moisture. Australian Journal of Grape and Wine Research, 16, 131-137. doi:10.1111/j.1755-0238.2009.00078.xComas, L. H., Anderson, L. J., Dunst, R. M., Lakso, A. N., & Eissenstat, D. M. (2005). Canopy and environmental control of root dynamics in a long‐term study of Concord grape. New Phytologist, 167(3), 829-840. doi:10.1111/j.1469-8137.2005.01456.xComont, G., Corio-Costet, M.-F., Larignon, P., & Delmotte, F. (2010). AFLP markers reveal two genetic groups in the French population of the grapevine fungal pathogen Phaeomoniella chlamydospora. European Journal of Plant Pathology, 127(4), 451-464. doi:10.1007/s10658-010-9611-3Corso, M., & Bonghi, C. (2014). Grapevine rootstock effects on abiotic stress tolerance. Plant Science Today, 1(3), 108-113. doi:10.14719/pst.2014.1.3.64Corso, M., Vannozzi, A., Maza, E., Vitulo, N., Meggio, F., Pitacco, A., … Lucchin, M. (2015). Comprehensive transcript profiling of two grapevine rootstock genotypes contrasting in drought susceptibility links the phenylpropanoid pathway to enhanced tolerance. Journal of Experimental Botany, 66(19), 5739-5752. doi:10.1093/jxb/erv274Costa, J. M., Vaz, M., Escalona, J., Egipto, R., Lopes, C., Medrano, H., & Chaves, M. M. (2016). Modern viticulture in southern Europe: Vulnerabilities and strategies for adaptation to water scarcity. Agricultural Water Management, 164, 5-18. doi:10.1016/j.agwat.2015.08.021Cousins, P. (2005). Rootstock Breeding: An Analysis of Intractability. HortScience, 40(7), 1945-1946. doi:10.21273/hortsci.40.7.1945Cramer W. Guiot J.andMarini K.(2019)MedECC booklet: risks associated to climate and environmental changes in the Mediterranean region. A preliminary assessment by the MedECC Network Science‐policy interface.https://www.medecc.org/wp-content/uploads/2018/12/MedECC-Booklet_EN_WEB.pdfCummins, J. N., & Aldwinckle, H. S. (1995). Breeding rootstocks for tree fruit crops. New Zealand Journal of Crop and Horticultural Science, 23(4), 395-402. doi:10.1080/01140671.1995.9513915Davies, W. J., Kudoyarova, G., & Hartung, W. (2005). Long-distance ABA Signaling and Its Relation to Other Signaling Pathways in the Detection of Soil Drying and the Mediation of the Plant’s Response to Drought. Journal of Plant Growth Regulation, 24(4). doi:10.1007/s00344-005-0103-1Degu, A., Morcia, C., Tumino, G., Hochberg, U., Toubiana, D., Mattivi, F., … Fait, A. (2015). Metabolite profiling elucidates communalities and differences in the polyphenol biosynthetic pathways of red and white Muscat genotypes. Plant Physiology and Biochemistry, 86, 24-33. doi:10.1016/j.plaphy.2014.11.006Delrot, S., Grimplet, J., Carbonell-Bejerano, P., Schwandner, A., Bert, P.-F., Bavaresco, L., … Vezzulli, S. (2020). Genetic and Genomic Approaches for Adaptation of Grapevine to Climate Change. Genomic Designing of Climate-Smart Fruit Crops, 157-270. doi:10.1007/978-3-319-97946-5_7Demangeat, G., Voisin, R., Minot, J.-C., Bosselut, N., Fuchs, M., & Esmenjaud, D. (2005). Survival of Xiphinema index in Vineyard Soil and Retention of Grapevine fanleaf virus Over Extended Time in the Absence of Host Plants. Phytopathology®, 95(10), 1151-1156. doi:10.1094/phyto-95-1151Downton, W. (1977). Photosynthesis in Salt-Stressed Grapevines. Functional Plant Biology, 4(2), 183. doi:10.1071/pp9770183Dutt, M., Li, Z. T., Kelley, K. T., Dhekney, S. A., Van Aman, M., Tattersall, J., & Gray, D. J. (2007). TRANSGENIC ROOTSTOCK PROTEIN TRANSMISSION IN GRAPEVINES. Acta Horticulturae, (738), 749-754. doi:10.17660/actahortic.2007.738.99Eissenstat, D. M., Bauerle, T. L., Comas, L. H., Lakso, A. N., Neilsen, D., Neilsen, G. H., & Smart, D. R. (2006). SEASONAL PATTERNS OF ROOT GROWTH IN RELATION TO SHOOT PHENOLOGY IN GRAPE AND APPLE. Acta Horticulturae, (721), 21-26. doi:10.17660/actahortic.2006.721.1ESCALONA, J. M., TOMÀS, M., MARTORELL, S., MEDRANO, H., RIBAS-CARBO, M., & FLEXAS, J. (2012). Carbon balance in grapevines under different soil water supply: importance of whole plant respiration. Australian Journal of Grape and Wine Research, 18(3), 308-318. doi:10.1111/j.1755-0238.2012.00193.xEsmenjaud, D., & Bouquet, A. (2009). Selection and Application of Resistant Germplasm for Grapevine Nematodes Management. Integrated Management of Fruit Crops Nematodes, 195-214. doi:10.1007/978-1-4020-9858-1_8Fahrentrapp, J., Müller, L., & Schumacher, P. (2015). Is there need for leaf-galling grape phylloxera control? Presence and distribution ofDactulosphaira vitifoliaein Swiss vineyards. International Journal of Pest Management, 61(4), 340-345. doi:10.1080/09670874.2015.1067734FLEXAS, J., GALMÃ S, J., GALLÃ , A., GULÃ AS, J., POU, A., RIBAS-CARBO, M., … MEDRANO, H. (2010). Improving water use efficiency in grapevines: potential physiological targets for biotechnological improvement. Australian Journal of Grape and Wine Research, 16, 106-121. doi:10.1111/j.1755-0238.2009.00057.xFort, K. P., Heinitz, C. C., & Walker, M. A. (2015). Chloride exclusion patterns in six grapevine populations. Australian Journal of Grape and Wine Research, 21(1), 147-155. doi:10.1111/ajgw.12125Foundation Plant Services(2020) Grape Variery: RS‐2. Grape program at Foundation Plant Services.https://fps.ucdavis.edu/Fraga, H., Malheiro, A. C., Moutinho‐Pereira, J., & Santos, J. A. (2012). An overview of climate change impacts on European viticulture. Food and Energy Security, 1(2), 94-110. doi:10.1002/fes3.14Franck, N., Morales, J. P., Arancibia‐Avendaño, D., García de Cortázar, V., Perez‐Quezada, J. F., Zurita‐Silva, A., & Pastenes, C. (2011). Seasonal fluctuations in Vitis vinifera root respiration in the field. New Phytologist, 192(4), 939-951. doi:10.1111/j.1469-8137.2011.03860.xFu, Q., Tan, Y., Zhai, H., & Du, Y. (2019). Evaluation of salt resistance mechanisms of grapevine hybrid rootstocks. Scientia Horticulturae, 243, 148-158. doi:10.1016/j.scienta.2018.07.034Funes, I., Savé, R., Rovira, P., Molowny-Horas, R., Alcañiz, J. M., Ascaso, E., … Vayreda, J. (2019). Agricultural soil organic carbon stocks in the north-eastern Iberian Peninsula: Drivers and spatial variability. Science of The Total Environment, 668, 283-294. doi:10.1016/j.scitotenv.2019.02.317Galbignani, M., Merli, M. C., Magnanini, E., Bernizzoni, F., Talaverano, I., Gatti, M., … Poni, S. (2016). Gas exchange and water-use efficiency of cv. Sangiovese grafted to rootstocks of varying water-deficit tolerance. Irrigation Science, 34(2), 105-116. doi:10.1007/s00271-016-0490-zGambetta, G. A., Manuck, C. M., Drucker, S. T., Shaghasi, T., Fort, K., Matthews, M. A., … McElrone, A. J. (2012). The relationship between root hydraulics and scion vigour across Vitis rootstocks: what role do root aquaporins play? Journal of Experimental Botany, 63(18), 6445-6455. doi:10.1093/jxb/ers312Geier, T., Eimert, K., Scherer, R., & Nickel, C. (2008). Production and rooting behaviour of rolB-transgenic plants of grape rootstock ‘Richter 110’ (Vitis berlandieri × V. rupestris). Plant Cell, Tissue and Organ Culture, 94(3), 269-280. doi:10.1007/s11240-008-9352-6Girollet, N., Rubio, B., Lopez-Roques, C., Valière, S., Ollat, N., & Bert, P.-F. (2019). De novo phased assembly of the Vitis riparia grape genome. Scientific Data, 6(1). doi:10.1038/s41597-019-0133-3Gómez, J., Lasanta, C., Palacios-Santander, J. M., & Cubillana-Aguilera, L. M. (2015). Chemical modeling for pH prediction of acidified musts with gypsum and tartaric acid in warm regions. Food Chemistry, 168, 218-224. doi:10.1016/j.foodchem.2014.07.058Gong, H., Blackmore, D., Clingeleffer, P., Sykes, S., Jha, D., Tester, M., & Walker, R. (2010). Contrast in chloride exclusion between two grapevine genotypes and its variation in their hybrid progeny. Journal of Experimental Botany, 62(3), 989-999. doi:10.1093/jxb/erq326Gramaje, D., & Armengol, J. (2011). Fungal Trunk Pathogens in the Grapevine Propagation Process: Potential Inoculum Sources, Detection, Identification, and Management Strategies. Plant Disease, 95(9), 1040-1055. doi:10.1094/pdis-01-11-0025Gramaje, D., Armengol, J., & Ridgway, H. J. (2012). Genetic and virulence diversity, and mating type distribution of Togninia minima causing grapevine trunk diseases in Spain. European Journal of Plant Pathology, 135(4), 727-743. doi:10.1007/s10658-012-0110-6Gramaje, D., García-Jiménez, J., & Armengol, J. (2010). Field Evaluation of Grapevine Rootstocks Inoculated with Fungi Associated with Petri Disease and Esca. American Journal of Enology and Viticulture, 61(4), 512-520. doi:10.5344/ajev.2010.10021Gramaje, D., Úrbez-Torres, J. R., & Sosnowski, M. R. (2018). Managing Grapevine Trunk Diseases With Respect to Etiology and Epidemiology: Current Strategies and Future Prospects. Plant Disease, 102(1), 12-39. doi:10.1094/pdis-04-17-0512-feGramaje, D., Mostert, L., Groenewald, J. Z., & Crous, P. W. (2015). Phaeoacremonium: From esca disease to phaeohyphomycosis. Fungal Biology, 119(9), 759-783. doi:10.1016/j.funbio.2015.06.004Gramaje, D., León, M., Santana, M., Crous, P. W., & Armengol, J. (2014). Multilocus ISSR Markers Reveal Two Major Genetic Groups in Spanish and South African Populations of the Grapevine Fungal Pathogen Cadophora luteo-olivacea. PLoS ONE, 9(10), e110417. doi:10.1371/journal.pone.0110417Granett, J., Walker, M. A., Kocsis, L., & Omer, A. D. (2001). BIOLOGY AND MANAGEMENT OF GRAPE PHYLLOXERA. Annual Review of Entomology, 46(1), 387-412. doi:10.1146/annurev.ento.46.1.387Gubler, W. D., Baumgartner, K., Browne, G. T., Eskalen, A., Latham, S. R., Petit, E., & Bayramian, L. A. (2004). Root diseases of grapevines in California and their control. Australasian Plant Pathology, 33(2), 157. doi:10.1071/ap04019Gullo, G., Dattola, A., Vonella, V., & Zappia, R. (2018). Evaluation of water relation parameters in vitis rootstocks with different drought tolerance and their effects on growth of a grafted cultivar. Journal of Plant Physiology, 226, 172-178. doi:10.1016/j.jplph.2018.04.013Haider, M. S., Jogaiah, S., Pervaiz, T., Yanxue, Z., Khan, N., & Fang, J. (2019). Physiological and transcriptional variations inducing complex adaptive mechanisms in grapevine by salt stress. Environmental and Experimental Botany, 162, 455-467. doi:10.1016/j.envexpbot.2019.03.022Hajdu, E. (2015). Grapevine breeding in Hungary. Grapevine Breeding Programs for the Wine Industry, 103-134. doi:10.1016/b978-1-78242-075-0.00006-5Harbertson, J. F., & Keller, M. (2011). Rootstock Effects on Deficit-Irrigated Winegrapes in a Dry Climate: Grape and Wine Composition. American Journal of Enology and Viticulture, 63(1), 40-48. doi:10.5344/ajev.2011.11079Haywood, V., Yu, T.-S., Huang, N.-C., & Lucas, W. J. (2005). Phloem long-distance trafficking of GIBBERELLIC ACID-INSENSITIVE RNA regulates leaf development. The Plant Journal, 42(1), 49-68. doi:10.1111/j.1365-313x.2005.02351.xHe, F., Mu, L., Yan, G.-L., Liang, N.-N., Pan, Q.-H., Wang, J., … Duan, C.-Q. (2010). Biosynthesis of Anthocyanins and Their Regulation in Colored Grapes. Molecules, 15(12), 9057-9091. doi:10.3390/molecules15129057He, R., Zhuang, Y., Cai, Y., Agüero, C. B., Liu, S., Wu, J., … Zhang, Y. (2018). Overexpression of 9-cis-Epoxycarotenoid Dioxygenase Cisgene in Grapevine Increases Drought Tolerance and Results in Pleiotropic Effects. Frontiers in Plant Science, 9. doi:10.3389/fpls.2018.00970Heinitz, C. C., Riaz, S., Tenscher, A. C., Romero, N., & Walker, M. A. (2020). Survey of chloride exclusion in grape germplasm from the southwestern United States and Mexico. Crop Science, 60(4), 1946-1956. doi:10.1002/csc2.20085Hemmer, C., Djennane, S., Ackerer, L., Hleibieh, K., Marmonier, A., Gersch, S., … Ritzenthaler, C. (2017). Nanobody-mediated resistance to Grapevine fanleaf virus in plants. Plant Biotechnology Journal, 16(2), 660-671. doi:10.1111/pbi.12819Henderson, S. W., Baumann, U., Blackmore, D. H., Walker, A. R., Walker, R. R., & Gilliham, M. (2014). Shoot chloride exclusion and salt tolerance in grapevine is associated with differential ion transporter expression in roots. BMC Plant Biology, 14(1). doi:10.1186/s12870-014-0273-8Henderson, S. W., Dunlevy, J. D., Wu, Y., Blackmore, D. H., Walker, R. R., Edwards, E. J., … Walker, A. R. (2017). Functional differences in transport properties of natural HKT 1;1 variants influence shoot Na + exclusion in grapevine rootstocks. New Phytologist, 217(3), 1113-1127. doi:10.1111/nph.14888Hernández-Montes, E., Escalona, J. M., Tomás, M., & Medrano, H. (2017). Influence of water availability and grapevine phenological stage on the spatial variation in soil respiration. Australian Journal of Grape and Wine Research, 23(2), 273-279. doi:10.1111/ajgw.12279De Herralde, F., Sa

Challenges of viticulture adaptation to global change: tackling the issue from the roots

Viticulture is facing emerging challenges not only because of the effect of climate change on yield and composition of grapes, but also of a social demand for environmental-friendly agricultural management. Adaptation to these challenges is essential to guarantee the sustainability of viticulture. The aim of this review is to present adaptation possibilities from the soil-hidden, and often disregarded, part of the grapevine, the roots. The complexity of soil-root interactions makes necessary a comprehensive approach taking into account physiology, pathology and genetics, in order to outline strategies to improve viticulture adaptation to current and future threats. Rootstocks are the link between soil and scion in grafted crops, and they have played an essential role in viticulture since the introduction of phylloxera into Europe at the end of the 19th century. This review outlines current and future challenges that are threatening the sustainability of the wine sector and the relevant role that rootstocks can play to face these threats. We describe how rootstocks along with soil management can be exploited as an essential tool to deal with the effects of climate change and of emerging soil-borne pests and pathogens. Moreover, we discuss the possibilities and limitations of diverse genetic strategies for rootstock breeding.This work is framed in the networking activities of RedVitis (AGL2015-70931-REDT) and RedVitis 2.0 (AGL2017-90759-REDT), funded by the State Research Agency (AEI) of the Spanish Ministry of Science and Innovation. Ms Diana Marin is beneficiary of postgraduate scholarship funded by Universidad Publica de Navarra (FPI-UPNA-2016). Dr Juan Emilio Palomares-Rius acknowledges the State Research Agency (AEI) of the Spanish Ministry of Science and Innovation for the 'Ramon y Cajal' Fellowship RYC-2017-22228 and Dr David Gramaje acknowledges Spanish Ministry of Economy and Competitiveness for the 'Ramon y Cajal' Fellowship RYC-2017-23098

A Regulatory Network for Coordinated Flower Maturation

For self-pollinating plants to reproduce, male and female organ development must be coordinated as flowers mature. The Arabidopsis transcription factors AUXIN RESPONSE FACTOR 6 (ARF6) and ARF8 regulate this complex process by promoting petal expansion, stamen filament elongation, anther dehiscence, and gynoecium maturation, thereby ensuring that pollen released from the anthers is deposited on the stigma of a receptive gynoecium. ARF6 and ARF8 induce jasmonate production, which in turn triggers expression of MYB21 and MYB24, encoding R2R3 MYB transcription factors that promote petal and stamen growth. To understand the dynamics of this flower maturation regulatory network, we have characterized morphological, chemical, and global gene expression phenotypes of arf, myb, and jasmonate pathway mutant flowers. We found that MYB21 and MYB24 promoted not only petal and stamen development but also gynoecium growth. As well as regulating reproductive competence, both the ARF and MYB factors promoted nectary development or function and volatile sesquiterpene production, which may attract insect pollinators and/or repel pathogens. Mutants lacking jasmonate synthesis or response had decreased MYB21 expression and stamen and petal growth at the stage when flowers normally open, but had increased MYB21 expression in petals of older flowers, resulting in renewed and persistent petal expansion at later stages. Both auxin response and jasmonate synthesis promoted positive feedbacks that may ensure rapid petal and stamen growth as flowers open. MYB21 also fed back negatively on expression of jasmonate biosynthesis pathway genes to decrease flower jasmonate level, which correlated with termination of growth after flowers have opened. These dynamic feedbacks may promote timely, coordinated, and transient growth of flower organs

Solar ultraviolet radiation and ozone depletion-driven climate change: Effects on terrestrial ecosystems

In this assessment we summarise advances in our knowledge of how UV-B radiation (280-315 nm), together with other climate change factors, influence terrestrial organisms and ecosystems. We identify key uncertainties and knowledge gaps that limit our ability to fully evaluate the interactive effects of ozone depletion and climate change on these systems. We also evaluate the biological consequences of the way in which stratospheric ozone depletion has contributed to climate change in the Southern Hemisphere. Since the last assessment, several new findings or insights have emerged or been strengthened. These include: (1) the increasing recognition that UV-B radiation has specific regulatory roles in plant growth and development that in turn can have beneficial consequences for plant productivity via effects on plant hardiness, enhanced plant resistance to herbivores and pathogens, and improved quality of agricultural products with subsequent implications for food security; (2) UV-B radiation together with UV-A (315-400 nm) and visible (400-700 nm) radiation are significant drivers of decomposition of plant litter in globally important arid and semi-arid ecosystems, such as grasslands and deserts. This occurs through the process of photodegradation, which has implications for nutrient cycling and carbon storage, although considerable uncertainty exists in quantifying its regional and global biogeochemical significance; (3) UV radiation can contribute to climate change via its stimulation of volatile organic compounds from plants, plant litter and soils, although the magnitude, rates and spatial patterns of these emissions remain highly uncertain at present. UV-induced release of carbon from plant litter and soils may also contribute to global warming; and (4) depletion of ozone in the Southern Hemisphere modifies climate directly via effects on seasonal weather patterns (precipitation and wind) and these in turn have been linked to changes in the growth of plants across the Southern Hemisphere. Such research has broadened our understanding of the linkages that exist between the effects of ozone depletion, UV-B radiation and climate change on terrestrial ecosystems

Berry Flesh and Skin Ripening Features in Vitis vinifera as Assessed by Transcriptional Profiling

Background Ripening of fleshy fruit is a complex developmental process involving the differentiation of tissues with separate functions. During grapevine berry ripening important processes contributing to table and wine grape quality take place, some of them flesh- or skin-specific. In this study, transcriptional profiles throughout flesh and skin ripening were followed during two different seasons in a table grape cultivar ‘Muscat Hamburg’ to determine tissue-specific as well as common developmental programs. Methodology/Principal Findings Using an updated GrapeGen Affymetrix GeneChip® annotation based on grapevine 12×v1 gene predictions, 2188 differentially accumulated transcripts between flesh and skin and 2839 transcripts differentially accumulated throughout ripening in the same manner in both tissues were identified. Transcriptional profiles were dominated by changes at the beginning of veraison which affect both pericarp tissues, although frequently delayed or with lower intensity in the skin than in the flesh. Functional enrichment analysis identified the decay on biosynthetic processes, photosynthesis and transport as a major part of the program delayed in the skin. In addition, a higher number of functional categories, including several related to macromolecule transport and phenylpropanoid and lipid biosynthesis, were over-represented in transcripts accumulated to higher levels in the skin. Functional enrichment also indicated auxin, gibberellins and bHLH transcription factors to take part in the regulation of pre-veraison processes in the pericarp, whereas WRKY and C2H2 family transcription factors seems to more specifically participate in the regulation of skin and flesh ripening, respectively. Conclusions/Significance A transcriptomic analysis indicates that a large part of the ripening program is shared by both pericarp tissues despite some components are delayed in the skin. In addition, important tissue differences are present from early stages prior to the ripening onset including tissue-specific regulators. Altogether, these findings provide key elements to understand berry ripening and its differential regulation in flesh and skin.This study was financially supported by GrapeGen Project funded by Genoma España within a collaborative agreement with Genome Canada. The authors also thank The Ministerio de Ciencia e Innovacion for project BIO2008-03892 and a bilateral collaborative grant with Argentina (AR2009-0021). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewe

Environmental effects of ozone depletion, UV radiation and interactions with climate change : UNEP Environmental Effects Assessment Panel, update 2017

Peer reviewe