Revitalization Through Food: Bringing Back Smalltown, USA

What makes a small town in the middle of nowhere USA unique, and what does food have to do with it? The following paper will discuss the impact of food on the revitalization process of small towns in the United States. This paper will attempt to answer questions concerning the revitalization process, how to measure the success of revitalization, and how food turns a town into a destination. Sidney Alive, a non-profit downtown revitalization group located in Sidney, Ohio, will be presented as a case study to discuss what could be a real-life success story. This case study will evaluate the work being done, the goals of the organization, and give a first-hand look at a revitalization project in progress: as well as how to best measure that progress. Additionally, research of downtown revitalization trends and many aspects of food tourism that create a unique process in small towns throughout the United States will be evaluated through an interdisciplinary lens. The many trends in downtown revitalization and food related destinations, such as the uniqueness that is needed for success or the historical significance of food, are discussed in relation to one another. Overall, the following paper will work to create a better understanding of the relationship between food as a destination and downtown revitalization in small towns throughout the United States, following different trends, discussing real-life work, and evaluation through an interdisciplinary lens

Comparison of Salivary Cortisol Levels in Various Academic Majors Using Enzyme-Linked Immunosorbent Assay

Keywords: Cortisol, Stress, ELISA The purpose of this study was to examine the impact that different majors at Embry-Riddle Aeronautical University have on salivary cortisol levels. Cortisol is a glucocorticoid synthesized in the zona fasciculata of the adrenal cortex. Secretion of cortisol is regulated by a hypothalamic hormone and a pituitary hormone. Corticotropin-releasing hormone (CRH) is released from the hypothalamus, and adrenocorticotropic hormone (ACTH) is released from the pituitary gland. Cortisol is a stress hormone that is secreted in response to a physical or emotional stressor. Different academic majors were anticipated to have different salivary cortisol levels when tested with competitive enzyme-linked immunosorbent assay (ELISA). Competitive ELISA uses cortisol standards and salivary samples to compete with cortisol conjugated to horseradish peroxidase to detect binding sites on a microtitre plate with coated anti-Cortisol antibodies. Bounded cortisol enzyme conjugates are measured by the reaction to the peroxidase to the substrate tetramethylbenzidine (TMB). Optical density is read on a plate reader, and the amount of cortisol in the sample is inversely proportional to the amount of enzyme conjugate detected from the plate well. The characterization of cortisol levels is classified by utilizing a standard curve line. Preliminary data indicates that cortisol levels are drastically increased in engineering majors and during morning lectures or laboratory classes. Future studies will include screening of a larger student population size for a more statistically accurate screening

Effects of Microgravity on mutualistic bacteria

Changes in environmental conditions represent a challenge for all terrestrial organisms, including the organisms involved in mutualistic associations (when both organisms obtain a benefit from each other). Changes in environment might include fluctuations in gravity and microgravity which represents a new frontier for space biology research. In this study we utilized Vibrio fischeri, a beneficial symbiotic bacterium of squids and monocentrid fishes. First, Vibrio fischeri was grown under gravity and microgravity conditions using two microgravity analogs: A Rotary Cell Culture System (RCCS) and a 2D clinostat design. After 48 hours of growth, we examined crucial phenotypic changes that might affect bacterial physiology and phenotypic changes involved in host colonization, mutualism and virulence. Phenotypes studied include cell growth, biofilm formation, cell colony morphology, susceptibility to toxic radicals and antibiotic resistance profiles. Our study demonstrates an increase of virulence-related phenotypes on strains grown under microgravity conditions compared to those grown under gravity conditions in both analog systems. This study will provide a window to the adaptative bacterial changes and responses in an effort to shed light on understanding microbiome changes and adaptations in space

Occupational Therapy in Primary Care -- Barriers, Pivots, and Outcomes: A pilot study

ABSTRACT Purpose: Habits, roles, and routines are important behaviors that affect lifestyle and can lead to the development of chronic disease such as diabetes mellitus. Primary care physicians (PCPs) have limited time, suggesting an interprofessional team approach would be beneficial in helping patients modify behaviors for the prevention and treatment of chronic disease. Occupational therapists (OTs) specialize in addressing health behaviors, and literature supports including OTs on the primary care team to improve the self-management techniques of patients with chronic disease. This study was guided by Lifestyle Redesign®, an evidence-based program that addresses the health behaviors of patients with diabetes in primary care. We aimed to improve health outcomes by combining an OT-led lifestyle modification program with patient-centered treatment recommendations from an external interprofessional team. We hypothesized that the health outcomes of patients with diabetes in primary care would improve after participating in an OT-led interprofessional lifestyle modification program, and that reimbursement for services would be obtainable. Method: Two PCPs in community practice initially agreed to involve an OT on their primary care team. Due to reimbursement concerns, the PCPs later preferred to refer patients to receive the OT-led intervention at an offsite clinic, rather than onsite at the primary care practice. Health outcomes were measured pre- and post-intervention using industry standard assessments and biometrics. Descriptive and non-parametric statistics were used to evaluate change. Results: Thirty-one patients with diabetes were referred, and three (9.7%) participated in the offsite lifestyle modification program. While statistical significance was not achieved (p≥0.10), trends toward individual improvement were noted for occupational performance and satisfaction and empowerment, and reimbursement was received from the third-party payers. Conclusions: Patient participation in this study was consistent with participation rates for offsite behavioral health referrals. An offsite OT-led interprofessional lifestyle modification program demonstrated potential for meaningful change and services were reimbursed. These findings support practice redesign efforts to include OTs as part of an integrated primary care model. Offering onsite services will increase patient accessibility to care and improve referral completion rates, thus providing OTs the opportunity to further demonstrate the efficacy of delivering chronic disease management in primary care

Latewood Ring Width Reveals CE 1734 Felling Dates for Walker House Timbers In Tupelo, Mississippi, USA

Dendroarchaeology is under-represented in the Gulf Coastal Plain region of the United States (US), and at present, only three published studies have precision dated a collection of 18th–19th-century structures. In this study, we examined the tree-ring data from pine, poplar, and oak timbers used in the Walker House in Tupelo, Mississippi. The Walker House was constructed ca. the mid-1800s with timbers that appeared to be recycled from previous structures. In total, we examined 30 samples (16 pines, 8 oaks, and 6 poplars) from the attic and crawlspace. We cross-dated latewood ring growth from the attic pine samples to the period 1541–1734 (r = 0.52, t = 8.43, p \u3c 0.0001) using a 514-year longleaf pine (Pinus palustris Mill.) latewood reference chronology from southern Mississippi. The crawlspace oak samples produced a 57-year chronology that we dated against a white oak (Quercus alba L.) reference chronology from northeast Alabama to the period 1765–1822 (r = 0.36, t = 2.83, p \u3c 0.01). We were unable to cross-date the six poplar samples due to a lack of poplar reference chronologies in the region. Our findings have two important implications: (1) the pine material dated to 1734 represents the oldest dendroarchaeology-confirmed dating match for construction materials in the southeastern US, and (2) cross-dating latewood growth for southeastern US pine species produced statistically significant results, whereas total ring width failed to produce significant dating results

Aging in fragile X syndrome

Many studies have focused on the behavior and cognitive problems in young patients with fragile X syndrome (FXS), but there are no studies about the problems in aging for those with FXS. The discovery of the fragile X-associated tremor ataxia syndrome (FXTAS), a neurodegenerative disorder related to elevated FMR1-mRNA, in elderly men and some women with the premutation, intensified the need for aging studies in FXS. Approximately 40% of males with FXS have repeat size mosaicism and as a result, some of these individuals also have elevated levels of FMR1-mRNA which theoretically puts them at risk for FXTAS. Here, we have surveyed all of the aging patients with FXS that we have followed over the years to clarify the medical complications of aging seen in those with FXS. Data was collected from 62 individuals with the FXS full mutation (44 males; 18 females) who were at least 40 years old at their most recent clinical examination. We found that the five most frequent medical problems in these patients were neurological problems (38.7%), gastrointestinal problems (30.6%), obesity (28.8%), hypertension (24.2%) and heart problems (24.2%). Movement disorders were significantly different between males and females (38.6% vs.10.2%, p = 0.029). We did not find any differences in medical problems between those with a full mutation and those with mosaicism. Identification of medical problems associated with aging in FXS is important to establish appropriate recommendations for medical screening and treatment considerations

Cyanobacterial distributions along a physico-chemical gradient in the Northeastern Pacific Ocean

The cyanobacteria Prochlorococcus and Synechococcus are important marine primary producers. We explored their distributions and covariance along a physico-chemical gradient from coastal to open ocean waters in the Northeastern Pacific Ocean. An inter-annual pattern was delineated in the dynamic transition zone where upwelled and eastern boundary current waters mix, and two new Synechococcus clades, Eastern Pacific Clade (EPC) 1 and EPC2, were identified. By applying state-of-the-art phylogenetic analysis tools to bar-coded 16S amplicon datasets, we observed higher abundance of Prochlorococcus high-light I (HLI) and low-light I (LLI) in years when more oligotrophic water intruded farther inshore, while under stronger upwelling Synechococcus I and IV dominated. However, contributions of some cyanobacterial clades were proportionally relatively constant, e.g. Synechococcus EPC2. In addition to supporting observations that Prochlorococcus LLI thrive at higher irradiances than other LL taxa, the results suggest LLI tolerate lower temperatures than previously reported. The phylogenetic precision of our 16S rRNA gene analytical approach and depth of bar-coded sequencing also facilitated detection of clades at low abundance in unexpected places. These include Prochlorococcus at the coast and Cyanobium-related sequences offshore, although it remains unclear whether these came from resident or potentially advected cells. Our study enhances understanding of cyanobacterial distributions in an ecologically important eastern boundary system. © 2014 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd

A solution to limitations of cognitive testing in children with intellectual disabilities: the case of fragile X syndrome

Intelligence testing in children with intellectual disabilities (ID) has significant limitations. The normative samples of widely used intelligence tests, such as the Wechsler Intelligence Scales, rarely include an adequate number of subjects with ID needed to provide sensitive measurement in the very low ability range, and they are highly subject to floor effects. The IQ measurement problems in these children prevent characterization of strengths and weaknesses, poorer estimates of cognitive abilities in research applications, and in clinical settings, limited utility for assessment, prognosis estimation, and planning intervention. Here, we examined the sensitivity of the Wechsler Intelligence Scale for Children (WISC-III) in a large sample of children with fragile X syndrome (FXS), the most common cause of inherited ID. The WISC-III was administered to 217 children with FXS (age 6–17 years, 83 girls and 134 boys). Using raw norms data obtained with permission from the Psychological Corporation, we calculated normalized scores representing each participant’s actual deviation from the standardization sample using a z-score transformation. To validate this approach, we compared correlations between the new normalized scores versus the usual standard scores with a measure of adaptive behavior (Vineland Adaptive Behavior Scales) and with a genetic measure specific to FXS (FMR1 protein or FMRP). The distribution of WISC-III standard scores showed significant skewing with floor effects in a high proportion of participants, especially males (64.9%–94.0% across subtests). With the z-score normalization, the flooring problems were eliminated and scores were normally distributed. Furthermore, we found correlations between cognitive performance and adaptive behavior, and between cognition and FMRP that were very much improved when using these normalized scores in contrast to the usual standardized scores. The results of this study show that meaningful variation in intellectual ability in children with FXS, and probably other populations of children with neurodevelopmental disorders, is obscured by the usual translation of raw scores into standardized scores. A method of raw score transformation may improve the characterization of cognitive functioning in ID populations, especially for research applications

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements