Collaborative Governance dalam Pembangunan Masyarakat Perbatasan di Kabupaten Merauke

The development of border areas aims to realize the border paradigm as the homeland of the Republic of Indonesia and needs to be carried out comprehensively, in the sense not only through a welfare approach but also through a security and environmental approach. For this reason, collaborative governance is one of the concepts that can be applied in developing border areas. This research was conducted using a descriptive method and a qualitative approach in Sota District, which is an area directly adjacent to Papua New Guinea. The results of the study indicate that collaborative governance in the development of the RI-PNG border area in the Sota District has not gone well. Indicators of starting conditions and intermediate outcomes have been seen from several programs such as the formation of community groups for Pokos honey entrepreneurs, eucalyptus oil processors, and ant nest tea processors. However, when viewed from the indicators of the collaborative process and institutional design, there has not been an ideal collaboration between the government, the private sector, and universities. Where it can be seen that all community empowerment activities are still dominated by the government, in this case, the Regional Border Management Agency, the Natural Resources Conservation Center, the Wasur National Park Center, and Merauke Regency. Ideally, in a collaborative process, universities and the community should be involved as implementers and balancers of the programs provided by the government in improving the welfare of the community

Implementation of The Disaster Management Policy in The Sigi District (Study on Regional Disaster Management in The Sigi Regency)

The objective of this research is to find out the implementation of disaster management policies in Sigi Regency which are focused on the rehabilitation and reconstruction phase of the earthquake and liquefaction post-disaster in Sigi Regency, namely the program of providing assistance to repair houses and permanent housing development assistance which is indeed the responsibility of the Regional Disaster Management Agency of Sigi Regency. It was a qualitative research method. Data were collected through observation, interview, and documentation. Researcher conducted interviews to 10 (ten) informants consisted of elements of program implementers and program recipients with questions related to 4 (four) aspects of the model of policy implementation from Thomas B. Smith. The results of the research show that the ideal policy aspect are implemented well while the aspect of Target Group, Implementing Organizations and Environmental Factors are not implemented well. Therefore, it can be concluded that the implementation of the disaster management policy of the rehabilitation and reconstruction phase of the program of providing assistance to repair houses for the community has not been implemented well

Pemodelan data warehouse pada jurusan teknik informatika unikom

Informasi sangat dibutuhkan tidak hanya sebagai hasil rekapitulasi saja akan tetapi suatu informasi dapat digunakan untuk membantu dalam proses pengambilan keputusan bagi pihak manajerial maupun eksekutif. Program Studi Teknik Informatika ketika akan melaksanakan akreditasi menghadapi kesulitan dalam menghimpun informasi dikarena penyajian informasi didapat dari berbagai basis data dan file eksternal. Basis data dan file eksternal yang digunakan belum mempunyai struktur yang sama sehingga diperlukan lagi usaha untuk menyeragamkan data. Data warehouse adalah sebuah koleksi data yang berorientasi subjek, diintegrasikan, time-variant, dan non volatile untuk mendukung proses pembuatan manajemen pengambilan keputusan. Hasil dari data warehouse merupakan informasi hasil intisari dari berbagai macam basis data.Hasil penelitian menghasilkan fakta bahwa atribut data pada diagram relasi OLTP masih belum bisa memenuhi kebutuhan data yang ada pada diagram relasi data warehouse dengan membandingkan antara diagram relasi OLTP dengan diagram relasi data warehouse. Terdapat kekurangan data pada OLTP Program Studi Teknik Informatika UNIKOM yang mengakibatkan data pada data warehouse tidak bisa diisi. Dari hasil penelitian ini diharapkan nantinya akan ada integrasi data pada seluruh basis data yang berhubungan dengan data warehouse agar model data warehouse yang telah dibentuk dapat diimplementasi pada penelitian berikutny

Contribution of alcohol use in HIV/hepatitis C virus co-infection to all-cause and cause-specific mortality: A collaboration of cohort studies

Among persons with HIV (PWH), higher alcohol use and having hepatitis C virus (HCV) are separately associated with increased morbidity and mortality. We investigated whether the association between alcohol use and mortality among PWH is modified by HCV. Data were combined from European and North American cohorts of adult PWH who started antiretroviral therapy (ART). Self-reported alcohol use data, collected in diverse ways between cohorts, were converted to grams/day. Eligible PWH started ART during 2001-2017 and were followed from ART initiation for mortality. Interactions between the associations of baseline alcohol use (0, 0.1-20.0, >20.0 g/day) and HCV status were assessed using multivariable Cox models. Of 58,769 PWH, 29,711 (51%), 23,974 (41%) and 5084 (9%) self-reported alcohol use of 0 g/day, 0.1-20.0 g/day, and > 20.0 g/day, respectively, and 4799 (8%) had HCV at baseline. There were 844 deaths in 37,729 person-years and 2755 deaths in 443,121 person-years among those with and without HCV, respectively. Among PWH without HCV, adjusted hazard ratios (aHRs) for mortality were 1.18 (95% CI: 1.08-1.29) for 0.0 g/day and 1.84 (1.62-2.09) for >20.0 g/day compared with 0.1-20.0 g/day. This J-shaped pattern was absent among those with HCV: aHRs were 1.00 (0.86-1.17) for 0.0 g/day and 1.64 (1.33-2.02) for >20.0 g/day compared with 0.1-20.0 g/day (interaction p < .001). Among PWH without HCV, mortality was higher in both non-drinkers and heavy drinkers compared with moderate alcohol drinkers. Among those with HCV, mortality was higher in heavy drinkers but not non-drinkers, potentially due to differing reasons for not drinking (e.g. illness) between those with and without HCV

Integration of genome-wide association studies with biological knowledge identifies six novel genes related to kidney function

In conducting genome-wide association studies (GWAS), analytical approaches leveraging biological information may further understanding of the pathophysiology of clinical traits. To discover novel associations with estimated glomerular filtration rate (eGFR), a measure of kidney function, we developed a strategy for integrating prior biological knowledge into the existing GWAS data for eGFR from the CKDGen Consortium. Our strategy focuses on single nucleotide polymorphism (SNPs) in genes that are connected by functional evidence, determined by literature mining and gene ontology (GO) hierarchies, to genes near previously validated eGFR associations. It then requires association thresholds consistent with multiple testing, and finally evaluates novel candidates by independent replication. Among the samples of European ancestry, we identified a genome-wide significant SNP in FBXL20 (P = 5.6 × 10−9) in meta-analysis of all available data, and additional SNPs at the INHBC, LRP2, PLEKHA1, SLC3A2 and SLC7A6 genes meeting multiple-testing corrected significance for replication and overall P-values of 4.5 × 10−4-2.2 × 10−7. Neither the novel PLEKHA1 nor FBXL20 associations, both further supported by association with eGFR among African Americans and with transcript abundance, would have been implicated by eGFR candidate gene approaches. LRP2, encoding the megalin receptor, was identified through connection with the previously known eGFR gene DAB2 and extends understanding of the megalin system in kidney function. These findings highlight integration of existing genome-wide association data with independent biological knowledge to uncover novel candidate eGFR associations, including candidates lacking known connections to kidney-specific pathways. The strategy may also be applicable to other clinical phenotypes, although more testing will be needed to assess its potential for discovery in genera

Genome-wide association and functional follow-up reveals new loci for kidney function

Chronic kidney disease (CKD) is an important public health problem with a genetic component. We performed genome-wide association studies in up to 130,600 European ancestry participants overall, and stratified for key CKD risk factors. We uncovered 6 new loci in association with estimated glomerular filtration rate (eGFR), the primary clinical measure of CKD, in or near MPPED2, DDX1, SLC47A1, CDK12, CASP9, and INO80. Morpholino knockdown of mpped2 and casp9 in zebrafish embryos revealed podocyte and tubular abnormalities with altered dextran clearance, suggesting a role for these genes in renal function. By providing new insights into genes that regulate renal function, these results could further our understanding of the pathogenesis of CKD

New genetic loci link adipose and insulin biology to body fat distribution.

Body fat distribution is a heritable trait and a well-established predictor of adverse metabolic outcomes, independent of overall adiposity. To increase our understanding of the genetic basis of body fat distribution and its molecular links to cardiometabolic traits, here we conduct genome-wide association meta-analyses of traits related to waist and hip circumferences in up to 224,459 individuals. We identify 49 loci (33 new) associated with waist-to-hip ratio adjusted for body mass index (BMI), and an additional 19 loci newly associated with related waist and hip circumference measures (P < 5 × 10(-8)). In total, 20 of the 49 waist-to-hip ratio adjusted for BMI loci show significant sexual dimorphism, 19 of which display a stronger effect in women. The identified loci were enriched for genes expressed in adipose tissue and for putative regulatory elements in adipocytes. Pathway analyses implicated adipogenesis, angiogenesis, transcriptional regulation and insulin resistance as processes affecting fat distribution, providing insight into potential pathophysiological mechanisms

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals &lt;1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Retrospective evaluation of whole exome and genome mutation calls in 746 cancer samples

Funder: NCI U24CA211006Abstract: The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) curated consensus somatic mutation calls using whole exome sequencing (WES) and whole genome sequencing (WGS), respectively. Here, as part of the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium, which aggregated whole genome sequencing data from 2,658 cancers across 38 tumour types, we compare WES and WGS side-by-side from 746 TCGA samples, finding that ~80% of mutations overlap in covered exonic regions. We estimate that low variant allele fraction (VAF < 15%) and clonal heterogeneity contribute up to 68% of private WGS mutations and 71% of private WES mutations. We observe that ~30% of private WGS mutations trace to mutations identified by a single variant caller in WES consensus efforts. WGS captures both ~50% more variation in exonic regions and un-observed mutations in loci with variable GC-content. Together, our analysis highlights technological divergences between two reproducible somatic variant detection efforts