Differential induction of chitinase in Piper colubrinum in response to inoculation with Phytophthora capsici, the cause of foot rot in black pepper

AbstractPlant chitinases have been of particular interest since they are known to be induced upon pathogen invasion. Inoculation of Piper colubrinum leaves with the foot rot fungus, Phytophthora capsici leads to increase in chitinase activity. A marked increase in chitinase activity in the inoculated leaves was observed, with the maximum activity after 60h of inoculation and gradually decreased thereafter. Older leaves showed more chitinase activity than young leaves. The level of chitinase in black pepper (Piper nigrum L.) upon inoculation was found to be substantially high when compared to P. colubrinum. RT–PCR using chitinase specific primers revealed differential accumulation of mRNA in P. colubrinum leaves inoculated with P. capsici. However, hyphal extension assays revealed no obvious differences in the ability of the protein extracts to inhibit growth of P. capsici in vitro

A method for isolation and reverse transcription of high quality RNA from Piper species

A rapid and efficient protocol for isolation of RNA from Piper leaves is described. RNA was extracted from two Piper species namel y, P. nigrum and P. colubrinulI1, using strong denaturing buffer containing guanidinum thiocyanate and polyvinylpyrollidone followed by p recipitation with pre-cooled sodium acetate and extraction with phenol:chloroform : isoamyl alcohol (25:24:1). RNA was precipitated with isopropanol. The extraction procedure reliably yielded high quality RNA suitable for reverse transcription experiments. &nbsp

Optimization of DNA isolation and PCR parameters in Myristica sp. and related gen- era for RAPD and ISSR analysis.

An efficient protocol for isolation of DNA from wild and related genera of Myristica rich inpolysaccharides and polyphenols was developed. The protocol utilizes CTAB (3%), 1.5% PVPand 0.3% ß-mercaptoethanol for isolation and RNase and phenol chloroform extraction forpurification. The yield of DNA ranged from 25-175 µg/g of fresh leaf tissue, with Knemaandamanica giving the highest yield. The present method yielded 10 times higher than theold methods. Characteristic patterns were generated on digestion of DNA by EcorI and HindIII restriction enzymes. PCR parameters were optimized using random primers (OPERONTechnology, USA). DNA concentration at 20 ng/reaction, annealing temperature of 45°C, 0.3mM dNTP in presence of 0.5 U of Taq DNA polymerase, and 2.0 mM MgCl2 and MJ ResearchGene Thermocycler was best. Successful amplification by ISSR and RAPD primers indicatedthat DNA is of good quality and free of polysaccharides and polyphenols. &nbsp

Trans-ancestry genome-wide association meta-analysis of prostate cancer identifies new susceptibility loci and informs genetic risk prediction.

Prostate cancer is a highly heritable disease with large disparities in incidence rates across ancestry populations. We conducted a multiancestry meta-analysis of prostate cancer genome-wide association studies (107,247 cases and 127,006 controls) and identified 86 new genetic risk variants independently associated with prostate cancer risk, bringing the total to 269 known risk variants. The top genetic risk score (GRS) decile was associated with odds ratios that ranged from 5.06 (95% confidence interval (CI), 4.84-5.29) for men of European ancestry to 3.74 (95% CI, 3.36-4.17) for men of African ancestry. Men of African ancestry were estimated to have a mean GRS that was 2.18-times higher (95% CI, 2.14-2.22), and men of East Asian ancestry 0.73-times lower (95% CI, 0.71-0.76), than men of European ancestry. These findings support the role of germline variation contributing to population differences in prostate cancer risk, with the GRS offering an approach for personalized risk prediction

Optimasi Portofolio Resiko Menggunakan Model Markowitz MVO Dikaitkan dengan Keterbatasan Manusia dalam Memprediksi Masa Depan dalam Perspektif Al-Qur`an

Risk portfolio on modern finance has become increasingly technical, requiring the use of sophisticated mathematical tools in both research and practice. Since companies cannot insure themselves completely against risk, as human incompetence in predicting the future precisely that written in Al-Quran surah Luqman verse 34, they have to manage it to yield an optimal portfolio. The objective here is to minimize the variance among all portfolios, or alternatively, to maximize expected return among all portfolios that has at least a certain expected return. Furthermore, this study focuses on optimizing risk portfolio so called Markowitz MVO (Mean-Variance Optimization). Some theoretical frameworks for analysis are arithmetic mean, geometric mean, variance, covariance, linear programming, and quadratic programming. Moreover, finding a minimum variance portfolio produces a convex quadratic programming, that is minimizing the objective function ðð¥with constraintsð ð 𥠥 ðandð´ð¥ = ð. The outcome of this research is the solution of optimal risk portofolio in some investments that could be finished smoothly using MATLAB R2007b software together with its graphic analysis

Search for heavy resonances decaying to two Higgs bosons in final states containing four b quarks

A search is presented for narrow heavy resonances X decaying into pairs of Higgs bosons (H) in proton-proton collisions collected by the CMS experiment at the LHC at root s = 8 TeV. The data correspond to an integrated luminosity of 19.7 fb(-1). The search considers HH resonances with masses between 1 and 3 TeV, having final states of two b quark pairs. Each Higgs boson is produced with large momentum, and the hadronization products of the pair of b quarks can usually be reconstructed as single large jets. The background from multijet and t (t) over bar events is significantly reduced by applying requirements related to the flavor of the jet, its mass, and its substructure. The signal would be identified as a peak on top of the dijet invariant mass spectrum of the remaining background events. No evidence is observed for such a signal. Upper limits obtained at 95 confidence level for the product of the production cross section and branching fraction sigma(gg -> X) B(X -> HH -> b (b) over barb (b) over bar) range from 10 to 1.5 fb for the mass of X from 1.15 to 2.0 TeV, significantly extending previous searches. For a warped extra dimension theory with amass scale Lambda(R) = 1 TeV, the data exclude radion scalar masses between 1.15 and 1.55 TeV

Search for supersymmetry in events with one lepton and multiple jets in proton-proton collisions at root s=13 TeV

Peer reviewe

Measurement of the top quark mass using charged particles in pp collisions at root s=8 TeV

Peer reviewe

A STUDY OF SOCIAL SUPPORT IN PHYSICALLY DISABLED STUDENTS IN AN EDUCATIONAL INSTITUTE OF KANPUR

Background- People with disabilities are the world’s largest minority, yet they are routinely excluded from most educational, economic and social opportunities. Persons with physical disabilities have to face problems not only because of their handicap but also due to non- addressal of their socio-emotional needs. Objective- 1) To study the bio-social characteristics of physically disabled students. 2) To study the profile of social support in   physically disabled students. Study design – Cross-sectional study. Study setting – Dr. Ambedkar Institute of Technology for Handicapped, U.P., Kanpur. Study subject- All the physically disabled diploma stream students present at the time of data collection were taken as study subjects. Data was collected by interview method using a predesigned & pretested Questionnaire. Results- Physically disabled students predominantly belonged to age-group 18-20 years (47.62%), Hindu religion (95.24%), OBC Caste (47.14%) and social class V (51.90%) as per modified BG Prasad social classification. 88.57% of families provide required support to their physically disabled children, while 27.14%, 20.48% & 14.29% parents avoid taking them to social gatherings, make undue interference & show discriminatory behaviour respectively.87.14% of students were well behaved by their siblings, 87.14% students can rely on their friends and about same percentage of normal children interact well with them. 90.95% of the students were treated by community satisfactorily. Majority of the students (>50%) accepted that family economy was affected adversely by their disability. Conclusion- In the present study, majority of the students were getting support from their parents, siblings, friends and community at large