Geoestatística aplicada ao estudo das características físico-químicas do solo em áreas de Floresta Estacional Decidual

Methods of geostatistics were used in the identification of size and structure of space variability of some physiochemical attributes of soils under seasonal deciduous forest areas, which were called mature forest, secondary forest and “capoeirão”. The areas, located in Santa Tereza, RS, were sampled during the period of 2002 and 2003, comprising the soil classes: Argiluvic Chernosol, Cambisol Ta and Litholic Neosol. Systematic sampling was performed with regular spacing grid of points varying of 30 m for “capoeirão” and 40 m for mature and secondary forest, in an amount of 80 sample points in a total area of 7.34 ha. For each point, measurements "in situ" were made of the soil bulk density, clay content, pH, potassium, organic matter and phosphorus. The structure of space variability was identified through the variograms, being defined the needed parameters for the Kriging. The most variable attribute was the organic matter, being not adjusted to any model of variogram, while the pH was the least. In the analysis of the space variability structure through the geostatistics, the clay and bulk density, both in area of secondary forest, and pH in the experimental areas, had showed the biggest space dependence. The Gauss model for the clay attribute obtained the largest reach (a), being 400 m and 388 m in the areas of secondary and mature forest, respectively. The crossed validation showed accuracy in the variographic models adjustment, being the pH variable the one which presented the optimum performance, 0.87; 0.94 and 0.92 in the areas of mature forest, secondary forest and “capoeirão”, respectively.Utilizaram-se métodos da geoestatística na identificação do tamanho e da estrutura da variabilidade espacial de alguns atributos físico-químicos do solo em áreas de Floresta Estacional Decidual as quais foram denominadas floresta madura, floresta secundária e capoeirão. As áreas, localizadas no município de Santa Tereza, RS, foram amostradas durante o período de 2002 a 2003, compreendendo as classes de solo: Chernossolo Argilúvico, Cambissolo Ta e Neossolo Litólico. Realizou-se amostragem sistemática com grid de espaçamento regular entre os pontos variando de 30 m para o capoeirão e 40 m para floresta madura e secundária, totalizando oitenta pontos amostrais em uma área total de 7,34 ha. Foram coletadas amostras de solo "in situ" para análise em laboratório dos atributos densidade do solo, argila, pH, potássio, matéria orgânica e fósforo. A estrutura da variabilidade espacial foi identificada por meio dos semivariogramas, definindo-se os parâmetros necessários para a krigagem. O atributo mais variável foi a matéria orgânica, não obtendo ajuste a nenhum modelo teórico de semivariograma, enquanto que o pH foi o menor. Na análise de estrutura da variabilidade espacial por meio da geoestatística, a argila e densidade, ambas em área de floresta secundária, e o pH nas áreas experimentais apresentaram a maior dependência espacial. O modelo gaussiano para o atributo argila obteve o maior alcance da dependência espacial, sendo 400 e 388 m, nas áreas de floresta secundária e madura respectivamente. A validação cruzada mostrou acuracidade no ajuste dos modelos variográficos, tendo a variável pH apresentado o melhor desempenho, 0,87; 0,94 e 0,92 nas áreas de floresta madura, secundária e capoeirão respectivamente

Geostatistics applied to the study of soil physiochemical characteristics in seasonal deciduous forest areas

Utilizaram-se m\ue9todos da geoestat\uedstica na identifica\ue7\ue3o do tamanho e da estrutura da variabilidade espacial de alguns atributos f\uedsico-qu\uedmicos do solo em \ue1reas de Floresta Estacional Decidual as quais foram denominadas floresta madura, floresta secund\ue1ria e capoeir\ue3o. As \ue1reas, localizadas no munic\uedpio de Santa Tereza, RS, foram amostradas durante o per\uedodo de 2002 a 2003, compreendendo as classes de solo: Chernossolo Argil\ufavico, Cambissolo Ta e Neossolo Lit\uf3lico. Realizou-se amostragem sistem\ue1tica com grid de espa\ue7amento regular entre os pontos variando de 30 m para o capoeir\ue3o e 40 m para floresta madura e secund\ue1ria, totalizando oitenta pontos amostrais em uma \ue1rea total de 7,34 ha. Foram coletadas amostras de solo \u201cin situ\u201d para an\ue1lise em laborat\uf3rio dos atributos densidade do solo, argila, pH, pot\ue1ssio, mat\ue9ria org\ue2nica e f\uf3sforo. A estrutura da variabilidade espacial foi identificada por meio dos semivariogramas, definindo-se os par\ue2metros necess\ue1rios para a krigagem. O atributo mais vari\ue1vel foi a mat\ue9ria org\ue2nica, n\ue3o obtendo ajuste a nenhum modelo te\uf3rico de semivariograma, enquanto que o pH foi o menor. Na an\ue1lise de estrutura da variabilidade espacial por meio da geoestat\uedstica, a argila e densidade, ambas em \ue1rea de floresta secund\ue1ria, e o pH nas \ue1reas experimentais apresentaram a maior depend\ueancia espacial. O modelo gaussiano para o atributo argila obteve o maior alcance da depend\ueancia espacial, sendo 400 e 388 m, nas \ue1reas de floresta secund\ue1ria e madura respectivamente. A valida\ue7\ue3o cruzada mostrou acuracidade no ajuste dos modelos variogr\ue1ficos, tendo a vari\ue1vel pH apresentado o melhor desempenho, 0,87; 0,94 e 0,92 nas \ue1reas de floresta madura, secund\ue1ria e capoeir\ue3o respectivamente.Methods of geostatistics were used in the identification of size and structure of space variability of some physiochemical attributes of soils under seasonal deciduous forest areas, which were called mature forest, secondary forest and "capoeir\ue3o". The areas, located in Santa Tereza, RS, were sampled during the period of 2002 and 2003, comprising the soil classes: Argiluvic Chernosol, Cambisol Ta and Litholic Neosol. Systematic sampling was performed with regular spacing grid of points varying of 30 m for "capoeir\ue3o" and 40 m for mature and secondary forest, in an amount of 80 sample points in a total area of 7.34 ha. For each point, measurements "in situ" were made of the soil bulk density, clay content, pH, potassium, organic matter and phosphorus. The structure of space variability was identified through the variograms, being defined the needed parameters for the Kriging. The most variable attribute was the organic matter, being not adjusted to any model of variogram, while the pH was the least. In the analysis of the space variability structure through the geostatistics, the clay and bulk density, both in area of secondary forest, and pH in the experimental areas, had showed the biggest space dependence. The Gauss model for the clay attribute obtained the largest reach (a), being 400 m and 388 m in the areas of secondary and mature forest, respectively. The crossed validation showed accuracy in the variographic models adjustment, being the pH variable the one which presented the optimum performance, 0.87; 0.94 and 0.92 in the areas of mature forest, secondary forest and "capoeir\ue3o", respectively

Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli

Background: Trypanosoma rangeli is a hemoflagellate protozoan parasite infecting humans and other wild and domestic mammals across Central and South America. It does not cause human disease, but it can be mistaken for the etiologic agent of Chagas disease, Trypanosoma cruzi. We have sequenced the T. rangeli genome to provide new tools for elucidating the distinct and intriguing biology of this species and the key pathways related to interaction with its arthropod and mammalian hosts.  Methodology/Principal Findings: The T. rangeli haploid genome is ,24 Mb in length, and is the smallest and least repetitive trypanosomatid genome sequenced thus far. This parasite genome has shorter subtelomeric sequences compared to those of T. cruzi and T. brucei; displays intraspecific karyotype variability and lacks minichromosomes. Of the predicted 7,613 protein coding sequences, functional annotations could be determined for 2,415, while 5,043 are hypothetical proteins, some with evidence of protein expression. 7,101 genes (93%) are shared with other trypanosomatids that infect humans. An ortholog of the dcl2 gene involved in the T. brucei RNAi pathway was found in T. rangeli, but the RNAi machinery is non-functional since the other genes in this pathway are pseudogenized. T. rangeli is highly susceptible to oxidative stress, a phenotype that may be explained by a smaller number of anti-oxidant defense enzymes and heatshock proteins.  Conclusions/Significance: Phylogenetic comparison of nuclear and mitochondrial genes indicates that T. rangeli and T. cruzi are equidistant from T. brucei. In addition to revealing new aspects of trypanosome co-evolution within the vertebrate and invertebrate hosts, comparative genomic analysis with pathogenic trypanosomatids provides valuable new information that can be further explored with the aim of developing better diagnostic tools and/or therapeutic targets

Standalone vertex finding in the ATLAS muon spectrometer

A dedicated reconstruction algorithm to find decay vertices in the ATLAS muon spectrometer is presented. The algorithm searches the region just upstream of or inside the muon spectrometer volume for multi-particle vertices that originate from the decay of particles with long decay paths. The performance of the algorithm is evaluated using both a sample of simulated Higgs boson events, in which the Higgs boson decays to long-lived neutral particles that in turn decay to bbar b final states, and pp collision data at √s = 7 TeV collected with the ATLAS detector at the LHC during 2011

Measurements of Higgs boson production and couplings in diboson final states with the ATLAS detector at the LHC

Measurements are presented of production properties and couplings of the recently discovered Higgs boson using the decays into boson pairs, H →γ γ, H → Z Z∗ →4l and H →W W∗ →lνlν. The results are based on the complete pp collision data sample recorded by the ATLAS experiment at the CERN Large Hadron Collider at centre-of-mass energies of √s = 7 TeV and √s = 8 TeV, corresponding to an integrated luminosity of about 25 fb−1. Evidence for Higgs boson production through vector-boson fusion is reported. Results of combined fits probing Higgs boson couplings to fermions and bosons, as well as anomalous contributions to loop-induced production and decay modes, are presented. All measurements are consistent with expectations for the Standard Model Higgs boson

Measurement of the top quark pair cross section with ATLAS in pp collisions at √s=7 TeV using final states with an electron or a muon and a hadronically decaying τ lepton

A measurement of the cross section of top quark pair production in proton-proton collisions recorded with the ATLAS detector at the Large Hadron Collider at a centre-of-mass energy of 7 TeV is reported. The data sample used corresponds to an integrated luminosity of 2.05 fb -1. Events with an isolated electron or muon and a τ lepton decaying hadronically are used. In addition, a large missing transverse momentum and two or more energetic jets are required. At least one of the jets must be identified as originating from a b quark. The measured cross section, σtt-=186±13(stat.)±20(syst.)±7(lumi.) pb, is in good agreement with the Standard Model prediction

Measurement of the top quark-pair production cross section with ATLAS in pp collisions at \sqrt{s}=7\TeV

A measurement of the production cross-section for top quark pairs(\ttbar) in $pp$ collisions at \sqrt{s}=7 \TeV is presented using data recorded with the ATLAS detector at the Large Hadron Collider. Events are selected in two different topologies: single lepton (electron $e$ or muon $\mu$) with large missing transverse energy and at least four jets, and dilepton ($ee$, $\mu\mu$ or $e\mu$) with large missing transverse energy and at least two jets. In a data sample of 2.9 pb-1, 37 candidate events are observed in the single-lepton topology and 9 events in the dilepton topology. The corresponding expected backgrounds from non-\ttbar Standard Model processes are estimated using data-driven methods and determined to be $12.2 \pm 3.9$ events and $2.5 \pm 0.6$ events, respectively. The kinematic properties of the selected events are consistent with SM \ttbar production. The inclusive top quark pair production cross-section is measured to be \sigmattbar=145 \pm 31 ^{+42}_{-27} pb where the first uncertainty is statistical and the second systematic. The measurement agrees with perturbative QCD calculations.Comment: 30 pages plus author list (50 pages total), 9 figures, 11 tables, CERN-PH number and final journal adde

Hunt for new phenomena using large jet multiplicities and missing transverse momentum with ATLAS in 4.7 fb−1 of √s=7 TeV proton-proton collisions

Results are presented of a search for new particles decaying to large numbers of jets in association with missing transverse momentum, using 4.7 fb−1 of pp collision data at s√=7TeV collected by the ATLAS experiment at the Large Hadron Collider in 2011. The event selection requires missing transverse momentum, no isolated electrons or muons, and from ≥6 to ≥9 jets. No evidence is found for physics beyond the Standard Model. The results are interpreted in the context of a MSUGRA/CMSSM supersymmetric model, where, for large universal scalar mass m 0, gluino masses smaller than 840 GeV are excluded at the 95% confidence level, extending previously published limits. Within a simplified model containing only a gluino octet and a neutralino, gluino masses smaller than 870 GeV are similarly excluded for neutralino masses below 100 GeV

Advancing Eucalyptus genomics: identification and sequencing of lignin biosynthesis genes from deep-coverage BAC libraries

<p>Abstract</p> <p>Background</p> <p><it>Eucalyptus </it>species are among the most planted hardwoods in the world because of their rapid growth, adaptability and valuable wood properties. The development and integration of genomic resources into breeding practice will be increasingly important in the decades to come. Bacterial artificial chromosome (BAC) libraries are key genomic tools that enable positional cloning of important traits, synteny evaluation, and the development of genome framework physical maps for genetic linkage and genome sequencing.</p> <p>Results</p> <p>We describe the construction and characterization of two deep-coverage BAC libraries EG_Ba and EG_Bb obtained from nuclear DNA fragments of <it>E. grandis </it>(clone BRASUZ1) digested with <it>Hind</it>III and <it>BstY</it>I, respectively. Genome coverages of 17 and 15 haploid genome equivalents were estimated for EG_Ba and EG_Bb, respectively. Both libraries contained large inserts, with average sizes ranging from 135 Kb (Eg_Bb) to 157 Kb (Eg_Ba), very low extra-nuclear genome contamination providing a probability of finding a single copy gene ≥ 99.99%. Libraries were screened for the presence of several genes of interest <it>via </it>hybridizations to high-density BAC filters followed by PCR validation. Five selected BAC clones were sequenced and assembled using the Roche GS FLX technology providing the whole sequence of the <it>E. grandis </it>chloroplast genome, and complete genomic sequences of important lignin biosynthesis genes.</p> <p>Conclusions</p> <p>The two <it>E. grandis </it>BAC libraries described in this study represent an important milestone for the advancement of <it>Eucalyptus </it>genomics and forest tree research. These BAC resources have a highly redundant genome coverage (> 15×), contain large average inserts and have a very low percentage of clones with organellar DNA or empty vectors. These publicly available BAC libraries are thus suitable for a broad range of applications in genetic and genomic research in <it>Eucalyptus </it>and possibly in related species of <it>Myrtaceae</it>, including genome sequencing, gene isolation, functional and comparative genomics. Because they have been constructed using the same tree (<it>E. grandis </it>BRASUZ1) whose full genome is being sequenced, they should prove instrumental for assembly and gap filling of the upcoming <it>Eucalyptus </it>reference genome sequence.</p