Stop N\u27 Go: Save Time, Save Lives

A poster presented by Maddox Alexander, William He, James Rodgers, Sahil Konduru, Paige Omohundro and Brandon Smith for the class Business, Accounting, and Entrepreneurship.https://scholarworks.moreheadstate.edu/gsp_projects_2019/1011/thumbnail.jp

Questions on pure luminosity evolution for ellipticals

The explanation for the existence of an excess population of faint blue galaxies (FBGs) has been a mystery for nearly two decades, and remains one of the grand astronomical issues to date. Existing models cannot explain all of the observational data such as galaxy number counts in the optical and infrared passbands and the redshift distributions of galaxies. Here, by modelling the morphological number counts derived from the Hubble Space Telescope, as well as the number counts in optical and infrared passbands, and the redshift and color distributions of galaxies obtained from ground-based observations, we show that the `FBG problem' cannot be resolved if elliptical galaxies are assumed to have formed in an instantaneous burst of star formation at high redshift with no subsequent star formation events, which is just the conventional scenario for formation and evolution of ellipticals. There exist great discrepancies between the observed $B-K$ color distribution and the predicted distribution for ellipticals by such a pure luminosity evolution (PLE) model in the context of the conventional scenario. Neither can the mild evolution (i.e., the star formation events have lasted for a longer time than those of the instantaneous burst and passive evolution since the formation of galaxies) for ellipticals be accepted in the context of PLE assumption. The introduction of dust extinction also cannot save the PLE models. This conclusion holds for each of the three cosmological models under consideration: flat, open and $\Lambda$-dominated. Hence, our investigation suggests that PLE assumption for elliptical galaxies is questionable, and number evolution may be essential for ellipticals.Comment: 20pages (AASTeX), 10 eps figures, 4 tables, to appear in ApJ, 1999, Vol. 51

Visualization of Mad2 Dynamics at Kinetochores, along Spindle Fibers, and at Spindle Poles in Living Cells

The spindle checkpoint prevents errors in chromosome segregation by inhibiting anaphase onset until all chromosomes have aligned at the spindle equator through attachment of their sister kinetochores to microtubules from opposite spindle poles. A key checkpoint component is the mitotic arrest–deficient protein 2 (Mad2), which localizes to unattached kinetochores and inhibits activation of the anaphase-promoting complex (APC) through an interaction with Cdc20. Recent studies have suggested a catalytic model for kinetochore function where unattached kinetochores provide sites for assembling and releasing Mad2–Cdc20 complexes, which sequester Cdc20 and prevent it from activating the APC. To test this model, we examined Mad2 dynamics in living PtK1 cells that were either injected with fluorescently labeled Alexa 488-XMad2 or transfected with GFP-hMAD2. Real-time, digital imaging revealed fluorescent Mad2 localized to unattached kinetochores, spindle poles, and spindle fibers depending on the stage of mitosis. FRAP measurements showed that Mad2 is a transient component of unattached kinetochores, as predicted by the catalytic model, with a t1/2 of ∼24–28 s. Cells entered anaphase ∼10 min after Mad2 was no longer detectable on the kinetochores of the last chromosome to congress to the metaphase plate. Several observations indicate that Mad2 binding sites are translocated from kinetochores to spindle poles along microtubules. First, Mad2 that bound to sites on a kinetochore was dynamically stretched in both directions upon microtubule interactions, and Mad2 particles moved from kinetochores toward the poles. Second, spindle fiber and pole fluorescence disappeared upon Mad2 disappearance at the kinetochores. Third, ATP depletion resulted in microtubule-dependent depletion of Mad2 fluorescence at kinetochores and increased fluorescence at spindle poles. Finally, in normal cells, the half-life of Mad2 turnover at poles, 23 s, was similar to kinetochores. Thus, kinetochore-derived sites along spindle fibers and at spindle poles may also catalyze Mad2 inhibitory complex formation

Budding Yeast Chromosome Structure and Dynamics during Mitosis

Using green fluorescent protein probes and rapid acquisition of high-resolution fluorescence images, sister centromeres in budding yeast are found to be separated and oscillate between spindle poles before anaphase B spindle elongation. The rates of movement during these oscillations are similar to those of microtubule plus end dynamics. The degree of preanaphase separation varies widely, with infrequent centromere reassociations observed before anaphase. Centromeres are in a metaphase-like conformation, whereas chromosome arms are neither aligned nor separated before anaphase. Upon spindle elongation, centromere to pole movement (anaphase A) was synchronous for all centromeres and occurred coincident with or immediately after spindle pole separation (anaphase B). Chromatin proximal to the centromere is stretched poleward before and during anaphase onset. The stretched chromatin was observed to segregate to the spindle pole bodies at rates greater than centromere to pole movement, indicative of rapid elastic recoil between the chromosome arm and the centromere. These results indicate that the elastic properties of DNA play an as of yet undiscovered role in the poleward movement of chromosome arms

Microstructural evolution and trace element mobility in Witwatersrand pyrite

Microstructural analysis of pyrite from a single sample of Witwatersrand conglomerate indicates a complex deformation history involving components of both plastic and brittle deformation. Internal deformation associated with dislocation creep is heterogeneously developed within grains, shows no systematic relationship to bulk rock strain or the location of grain boundaries and is interpreted to represent an episode of pyrite deformation that predates the incorporation of detrital pyrite grains into the Central Rand conglomerates. In contrast, brittle deformation, manifest by grain fragmentation that transects dislocation-related microstructures, is spatially related to grain contacts and is interpreted to represent post-depositional deformation of the Central Rand conglomerates. Analysis of the low-angle boundaries associated with the early dislocation creep phase of deformation indicates the operation of {100} slip systems. However, some orientation boundaries have geometrical characteristics that are not consistent with simple {100} deformation.These boundaries may represent the combination of multiple slip systems or the operation of the previously unrecognized {120} slip system. These boundaries are associated with order of magnitude enrichments in As, Ni and Co that indicate a deformation control on the remobilization of trace elements within pyrite and a potential slip system control on the effectiveness of fast-diffusion pathways. The results confirm the importance of grain-scale elemental remobilization within pyrite prior to their incorporation into the Witwatersrand gold-bearing conglomerates. Since the relationship between gold and pyrite is intimately related to the trace element geochemistry of pyrite, the results have implications for the application of minor element geochemistry to ore deposit formation, suggest a reason for heterogeneous conductivity and localized gold precipitation in natural pyrite and provide a framework for improving mineral processing

Fine-structured multi-scaling long-range correlations in completely sequenced genomes—features, origin, and classification

The sequential organization of genomes, i.e. the relations between distant base pairs and regions within sequences, and its connection to the three-dimensional organization of genomes is still a largely unresolved problem. Long-range power-law correlations were found using correlation analysis on almost the entire observable scale of 132 completely sequenced chromosomes of 0.5 × 106 to 3.0 × 107 bp from Archaea, Bacteria, Arabidopsis thaliana, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Drosophila melanogaster, and Homo sapiens. The local correlation coefficients show a species-specific multi-scaling behaviour: close to random correlations on the scale of a few base pairs, a first maximum from 40 to 3,400 bp (for Arabidopsis thaliana and Drosophila melanogaster divided in two submaxima), and often a region of one or more second maxima from 105 to 3 × 105 bp. Within this multi-scaling behaviour, an additional fine-structure is present and attributable to codon usage in all except the human sequences, where it is related to nucleosomal binding. Computer-generated random sequences assuming a block organization of genomes, the codon usage, and nucleosomal binding explain these results. Mutation by sequence reshuffling destroyed all correlations. Thus, the stability of correlations seems to be evolutionarily tightly controlled and connected to the spatial genome organization, especially on large scales. In summary, genomes show a complex sequential organization related closely to their three-dimensional organization

Characteristics of Mesoscale Convective Systems over China and Its Vicinity Using Geostationary Satellite FY2

This study investigates mesoscale convective systems (MCSs) over China and its vicinity during the boreal warm season (May-August) from 2005 to 2012 based on data fromthe geostationary satellite Fengyun 2 (FY2) series. The authors classified and analyzed the quasi-circular and elongated MCSs on both large and small scales, including mesoscale convective complexes (MCCs), persistent elongated convective systems (PECSs), meso-ß circular convective systems (MßCCSs), meso-ß elongated convective system (MßECSs), and two additional types named small meso-ß circular convective systems (SMßCCSs) and small meso-ß elongated convective systems (SMßECSs). Results show that nearly 80% of the 8696 MCSs identified in this study fall into the elongated categories. Overall, MCSs occur mainly at three zonal bands with average latitudes around 208, 308, and 508N. The frequency of MCSs occurrences is maximized at the zonal band around 208N and decreases with increase in latitude. During the eight warm seasons, the period of peak systems occurrences is in July, followed decreasingly by June, August, and May. Mean while, fromMay to August three kinds of monthly variations are observed, which are clear northward migration, rapid increase, and persistent high frequency of MCS occurrences. Compared to MCSs in the United States, the four types of MCSs (MCCs, PECSs, MßCCSs, and MßECSs) are relatively smaller both in size and eccentricity but exhibit nearly equal life spans. Moreover, MCSs in both countries share similar positive correlations between their duration and maximum extent. Additionally, the diurnal cycles of MCSs in both countries are similar (local time) regarding the three stages of initiation, maturation, and termination

The impact of reward and punishment on skill learning depends on task demands

Reward and punishment motivate behavior, but it is unclear exactly how they impact skill performance and whether the effect varies across skills. The present study investigated the effect of reward and punishment in both a sequencing skill and a motor skill context. Participants trained on either a sequencing skill (serial reaction time task) or a motor skill (force-tracking task). Skill knowledge was tested immediately after training, and again 1 hour, 24-48 hours, and 30 days after training. We found a dissociation of the effects of reward and punishment on the tasks, primarily reflecting the impact of punishment. While punishment improved serial reaction time task performance, it impaired force-tracking task performance. In contrast to prior literature, neither reward nor punishment benefitted memory retention, arguing against the common assumption that reward ubiquitously benefits skill retention. Collectively, these results suggest that punishment impacts skilled behavior more than reward in a complex, task dependent fashion

Photonic hydrogel sensors

Analyte-sensitive hydrogels that incorporate optical structures have emerged as sensing platforms for point-of-care diagnostics. The optical properties of the hydrogel sensors can be rationally designed and fabricated through self-assembly, microfabrication or laser writing. The advantages of photonic hydrogel sensors over conventional assay formats include label-free, quantitative, reusable, and continuous measurement capability that can be integrated with equipment-free text or image display. This Review explains the operation principles of photonic hydrogel sensors, presents syntheses of stimuli-responsive polymers, and provides an overview of qualitative and quantitative readout technologies. Applications in clinical samples are discussed, and potential future directions are identified