Cartographic Calculation and Coordination in the Urbanisation of the Peripheral Slopes of Lima

The urbanisation of the peripheral slopes of Lima is often referred to in official discourse and the media as an informal/illegal process driven exclusively by the urban poor. However, a close examination of such process defies its understanding as occurring beyond the State, in violation of planning laws, or in the exclusive domain of the poor’s agency. Instead close engagements with regulatory frameworks and spatial outcomes compliant with planning norms are central features, since such practices shape local dwellers' entitlements to basic services, as well as their expectations on securing tenure. Notwithstanding that the slopes have been declared uninhabitable high-risk zones by the State, their occupation is occurring at an unprecedented rate, exposing an increasing number of inhabitants to hazardous living conditions. The thesis examines how and why this mode of urbanisation is enabled and sustained. In so doing, it offers analytical and methodological insights into contemporary urbanisation processes across the Global South. Borrowing from actor-network theory and institutional ethnography, the research takes a relational and socio-material perspective. It focuses on cartography - the maps and plans used on the slopes of Lima- to provide a transversal reading across 'black boxed' actors such as the 'State, 'communities' and 'land traffickers', and observe the engagement with the regulatory frameworks. Through an ethnography of cartographic practices, the thesis provides a novel methodology for bringing into view the processes, practices, alliances, and agency which are often invisible to policy makers, yet structure outcomes. The thesis demonstrates that peripheral urbanisation and planning need to be considered as socio-technical assemblages that have numerous and unexpected ways of interlinking. Unintended consequences, such as the production of risk, are outcomes of these assemblages. Consequently, planning research could do more to consider the technical as much as the political aspects of planning and interrogate the agency of materiality in urban processes. For policy makers and planners, a better understanding of the socio-technical configurations can guide their actions to rearrange these toward progressive agendas

Participatory Mapping to Disrupt Unjust Urban Trajectories in Lima

This chapter shares the experience of two action research projects ReMap Lima and cLIMA sin Riego, where mapping has been used with three main objectives: to make visible what is otherwise ‘invisible’; to open up dialogue between different stakeholders in the city and to arrive at concrete actions, collectively negotiated between citizens and policy makers. Two case study sites were chosen in Lima, Peru: Barrios Altos (BA) in the historic centre and José Carlos Mariátegui (JCM) at the edge of the city. The approach adopted applies a participatory action methodology based on grounded applications and advanced technologies for community-led mapping and visualisation. The chapter reflects upon three interrelated sites of the mapping process: the reading, writing and audiencing of maps and explores how these can provide opportunities to break away from the polar positions often established between Claimant/ marginalised group and the state, thus aiming to contribute to a process of spatial co-learning across typically confronted actors. The two case studies show different possibilities for interrogating the city to provide a spatially and socially grounded way of co-producing knowledge for action that can contribute to the planning of just urban futures

Urban risk readdressed: Bridging resilience-seeking practices in African cities

Throughout the Global South, urbanization is increasingly coupled with the production of risk accumulation cycles or urban “risk traps”, which are not exclusively driven but exacerbated by climate change. This is the case across many cities in sub Sahara Africa, where biophysical and socio-economic risk drivers combine to produce vicious cycles of unequal risk exposure and displacement, with severe impacts on the lives, livelihoods and assets of the urban poor and the city’s ecological and socio-economic future. Focusing on two case studies characterized by different approaches to the governance of disaster risk management (DRM) – Freetown (Sierra Leone) and Karonga (Malawi) – this chapter seeks to untangle the processes that drive risk accumulation over time and to appraise the resilience-seeking practices deployed and resources mobilized to mitigate, reduce, and prevent risk. It reflects on the findings from an action-research project conducted in the aforementioned cities, as part of a wider program entitled “Urban Africa Risk Knowledge” (Urban ARK). As such, it provides fresh insights into how the governance of urban resilience currently works in both contexts and on how to enhance the capacity to act of those most vulnerable to become trapped in risk accumulation cycles to disrupt these traps strategically, inclusively, and collectively. Our central argument is that the capacity of emerging DRM governance frameworks to disrupt urban risk traps is defined by the extent to which resilience-seeking is actually practiced in a relational way – that is acknowledging the multiple practices that converge in responding to risk and their relative capacities to disrupt the risk accumulation cycles that impact the most vulnerable. We further hypothesize that the differential ability of ongoing resilient-seeking practices to disrupt risk traps is shaped by the extent to which their governance expands the political space to enable abridged collective action among the urban poor, customary authorities, local governments and external agencies

Centralized injustices: understanding energy resilience in times of disruption in low-income settlements in Peru

What happens once people have electricity has received far less analytical and policy attention than the provision of the infrastructure itself. For low-income settlements that have gained a connection to the grid, energy access challenges can still prevail, keeping many inhabitants in energy poverty. This paper analyses energy practices in three low-income neighbourhoods in Lima, Peru, with particular attention to inhabitants’ responses in the face of the COVID-19 pandemic. In doing so, it seeks to draw lessons for energy policy and planning to enhance energy resilience in the transition towards more just and sustainable futures. Building on energy resilience scholarship and drawing links with justice debates, this paper discusses three community coping strategies: (1) fuel stacking; (2) collective practices and the shared economy; and (3) material and spatial changes. It analyses how these strategies relate to dominant policy directions, as well as their implications for energy resilience and justice more broadly

Effect of leaf area on maize productivity

Maize (Zea mays L) leaves provide energy for growth and development. Increases in plant densities the past 75 years have contributed to increased maize grain yields. No recorded change has been observed in leaf area per plant during this period, but some change may have occurred. Plant density increases are associated with in¬creases in leaf area per-unit of land mass. Grain yield increases resulted from hybrids with improved tolerance to higher plant densities. Recently developed maize hybrids have upright leaves and smaller tassels allowing more light to penetrate the leaf canopy. Tolerance to increased plant density is directly related to intra and inter-plant shading plus changes in leaf area per plant may change leaf canopy structure. To evaluate the concept, maize leaf area affects grain yield, we developed high- and low leaf area hybrids. Objectives were to evaluate productivity of high and low leaf area maize hybrids at three high plant densities for two years. Averaged over three plant densities low leaf area hybrids produced significantly more grain than high leaf area hybrids. Low leaf area hybrids tolerated higher plant density better than high leaf area hybrids. Results indicate low leaf area hybrids are superior in several maize productivity traits

Evasion of anti-growth signaling: a key step in tumorigenesis and potential target for treatment and prophylaxis by natural compounds

The evasion of anti-growth signaling is an important characteristic of cancer cells. In order to continue to proliferate, cancer cells must somehow uncouple themselves from the many signals that exist to slow down cell growth. Here, we define the anti-growth signaling process, and review several important pathways involved in growth signaling: p53, phosphatase and tensin homolog (PTEN), retinoblastoma protein (Rb), Hippo, growth differentiation factor 15 (GDF15), AT-rich interactive domain 1A (ARID1A), Notch, insulin-like growth factor (IGF), and Krüppel-like factor 5 (KLF5) pathways. Aberrations in these processes in cancer cells involve mutations and thus the suppression of genes that prevent growth, as well as mutation and activation of genes involved in driving cell growth. Using these pathways as examples, we prioritize molecular targets that might be leveraged to promote anti-growth signaling in cancer cells. Interestingly, naturally-occurring phytochemicals found in human diets (either singly or as mixtures) may promote anti-growth signaling, and do so without the potentially adverse effects associated with synthetic chemicals. We review examples of naturally-occurring phytochemicals that may be applied to prevent cancer by antagonizing growth signaling, and propose one phytochemical for each pathway. These are: epigallocatechin-3-gallate (EGCG) for the Rb pathway, luteolin for p53, curcumin for PTEN, porphyrins for Hippo, genistein for GDF15, resveratrol for ARID1A, withaferin A for Notch and diguelin for the IGF1-receptor pathway. The coordination of anti-growth signaling and natural compound studies will provide insight into the future application of these compounds in the clinical setting

Effects of antiplatelet therapy on stroke risk by brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases: subgroup analyses of the RESTART randomised, open-label trial

Background Findings from the RESTART trial suggest that starting antiplatelet therapy might reduce the risk of recurrent symptomatic intracerebral haemorrhage compared with avoiding antiplatelet therapy. Brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases (such as cerebral microbleeds) are associated with greater risks of recurrent intracerebral haemorrhage. We did subgroup analyses of the RESTART trial to explore whether these brain imaging features modify the effects of antiplatelet therapy

Loss of Function of the Nuclear Receptor NR2F2, Encoding COUP-TF2, Causes Testis Development and Cardiac Defects in 46,XX Children

Emerging evidence from murine studies suggests that mammalian sex determination is the outcome of an imbalance between mutually antagonistic male and female regulatory networks that canalize development down one pathway while actively repressing the other. However, in contrast to testis formation, the gene regulatory pathways governing mammalian ovary development have remained elusive. We performed exome or Sanger sequencing on 79 46,XX SRY-negative individuals with either unexplained virilization or with testicular/ovotesticular disorders/differences of sex development (TDSD/OTDSD). We identified heterozygous frameshift mutations in NR2F2, encoding COUP-TF2, in three children. One carried a c.103_109delGGCGCCC (p.Gly35Argfs( *)75) mutation, while two others carried a c.97_103delCCGCCCG (p.Pro33Alafs( *)77) mutation. In two of three children the mutation was de novo. All three children presented with congenital heart disease (CHD), one child with congenital diaphragmatic hernia (CDH), and two children with blepharophimosis-ptosis-epicanthus inversus syndrome (BPES). The three children had androgen production, virilization of external genitalia, and biochemical or histological evidence of testicular tissue. We demonstrate a highly significant association between the NR2F2 loss-of-function mutations and this syndromic form of DSD (p = 2.44 x 10(-8)). We show that COUP-TF2 is highly abundant in a FOXL2-negative stromal cell population of the fetal human ovary. In contrast to the mouse, these data establish COUP-TF2 as a human "pro-ovary" and "anti-testis" sex-determining factor in female gonads. Furthermore, the data presented here provide additional evidence of the emerging importance of nuclear receptors in establishing human ovarian identity and indicate that nuclear receptors may have divergent functions in mouse and human biology

Correlation between MMPs and their inhibitors in breast cancer tumor tissue specimens and in cell lines with different metastatic potential

Background: The metastatic disease rather than the primary tumor itself is responsible for death in most solid tumors, including breast cancer. The role of matrix metalloproteinases ( MMPs), tissue inhibitors of MMPs (TIMPs) and Reversion-inducing cysteine-rich protein with Kazal motifs ( RECK) in the metastatic process has previously been established. However, in all published studies only a limited number of MMPs/MMP inhibitors was analyzed in a limited number of cell lines. Here, we propose a more comprehensive approach by analyzing the expression levels of several MMPs (MMP-2, MMP-9 and MMP-14) and MMP inhibitors (TIMP-1, TIMP-2 and RECK) in different models ( five human breast cancer cell lines, 72 primary breast tumors and 30 adjacent normal tissues). Methods: We analyzed the expression levels of MMP-2, MMP-9 and MMP-14 and their inhibitors (TIMP-1, TIMP-2 and RECK) by quantitative RT-PCR (qRT-PCR) in five human breast cancer cell lines presenting increased invasiveness and metastatic potential, 72 primary breast tumors and 30 adjacent normal tissues. Moreover, the role of cell-extracellular matrix elements interactions in the regulation of expression and activity of MMPs and their inhibitors was analyzed by culturing these cell lines on plastic or on artificial ECM (Matrigel). Results: The results demonstrated that MMPs mRNA expression levels displayed a positive and statistically significant correlation with the transcriptional expression levels of their inhibitors both in the cell line models and in the tumor tissue samples. Furthermore, the expression of all MMP inhibitors was modulated by cell-Matrigel contact only in highly invasive and metastatic cell lines. The enzyme/inhibitor balance at the transcriptional level significantly favors the enzyme which is more evident in tumor than in adjacent non-tumor tissue samples. Conclusion: Our results suggest that the expression of MMPs and their inhibitors, at least at the transcriptional level, might be regulated by common factors and signaling pathways. Therefore, the multi-factorial analysis of these molecules could provide new and independent prognostic information contributing to the determination of more adequate therapy strategies for each patient.`Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Conselho Nacional de Pesquisa (CNPq)Financiadora de Estudos e Projetos (FINEP)Pro-Reitoria da Universidade de Sao Paulo (PRP-USP