Migratory history of wild and released ayu (Plecoglossus altivelis) in the Kurobe River, Japan

In order to discuss the influence of dam discharge on the movement of ayu Plecoglossus altivelis in a river and the stocking effectiveness of released ayu, the migratory history of 83 juveniles of ayu (standard length=67.9-142.4mm sL) collected in the Kurobe River, Japan during 25 June-23 August 2004 was determined by otolith microchemistry, and the origin of fish was identified as being either native amphidromous ayu or released amphidromous or landlocked fish. Except for otoliths with abnormal morphology (N=10), most specimens (96%, N=70) had inner layers (radius:>400μm) of higher Sr: Ca ratios and outer ones of low values. These specimens were identified as native amphidromous fish that migrated upstream from the sea. Only 4% (N=3) were released ayu (reared fish), which were further divided into two origins, amphidromous fish (N=2) with smaller inner layers (radius:<400μm) of high Sr: Ca ratios, and landlocked fish (N=1) with constantly low values over the whole otolith. There were no specimens with an increase in Sr: Ca ratios in the outer layers of the low Sr: Ca region of the otolith after their upstream migration in spring, suggesting that it is unlikely that any of these ayu had been washed-out from the river by cold and turbulent river water

Age and growth of Anguilla interioris leptocephali collected in Indonesian waters

Anguilla interiorisis has been only known to be distributed in northern New Guinea, but recent sampling surveys for leptocephali and the development of species identification techniques using DNA analysis have discovered that this species also appears to occur off Sumatra in the eastern Indian Ocean, and may be present in other areas of the Indonesian Archipelago. To reveal the ages and early life histories of this species, the otolith microstructure of the leptocephali collected near the Sulawesi Island and off Sumatra of Indonesia were examined. The otolith microstructure of the A. interioris leptocephali was similar to other anguillid species and showed narrow increment widths (0.49-0.58μm) near the core that increased (1.14-1.34μm) before decreasing again until the otolith edge (0.51-0.83μm), except in the smallest specimen (12.4mm TL) collected off Sumatra, which had no peak. The range of Sr: Ca ratios in their otoliths were 8.37 to 14.01. Their ages were 19d for the smallest specimen from off Sumatra, 85d for the specimen (48.9mm TL) from Tomini Bay, and 85 to 94d for the three specimens (43.4-46.5mm TL) from the Molucca Sea. The overall growth rate of the leptocephali was 0.48mm/d, and this value was intermediate compared with the growth rates of the other anguillid species. The age of the smallest A. interioris leptocephalus collected off Sumatra and the geographic patterns of currents in the regions, suggest that it was spawned in the Indian Ocean and that it may belong to a different population than the New Guinea population

Correspondence between otolith microstructual changes and early life history events in Anguilla marmorata leptocephali and glass eels

To determine the exact correspondence between otolith characteristics and early life history events such as metamorphosis, coastal migration and recruitment to estuaries, both otolith microstructure and microchemistry analyses were applied to a sequential developmental series of samples, e. g. leptocephali, a metamorphosing larva, oceanic glass eels, and coastal glass eels. Total length and age were 10.1-50.7 mm and 22-137 d in leptocephali, 46.3 mm and 147 d in a metamorphosing larva, 47.8, 48.6 mm and 159, 160 d in oceanic glass eels, and 47.9-54.8 mm and 119-168 d in coastal glass eels. Checks at hatching and first feeding were observed in all specimens, but metamorphosis and freshwater checks were observed only in some specimens. It was confirmed that the abrupt drop in otolith Sr : Ca ratios and drastic increases of otolith increment widths in the metamorphosing larval stage correspond to the onset of metamorphosis, and the decrease after the peaks suggested the completion of metamorphosis, because the metamorphosing larva had no decrease in incremental widths. The relatively conserved Sr : Ca ratios decreased sharply in synchrony with the increasing increment widths. This study provides the first direct evidence that these drastic changes in otolith microstructure and microchemistry actually occur during metamorphosis, which has been only hypothesized

Original delayed-start ovarian stimulation protocol with a gonadotropin-releasing hormone antagonist, medroxyprogesterone acetate, and high-dose gonadotropin for poor responders and patients with poor-quality embryos

IntroductionThe delayed-start gonadotropin-releasing hormone antagonist protocol seems effective for patients who are poor ovarian responders, but there are insufficient data on whether it is also effective for patients with poor-quality embryos and low rates of good blastocyst formation. Specifically, the effectiveness of delayed-start gonadotropin-releasing hormone antagonists with progesterone has not been adequately investigated. Therefore, we compared the efficacy of the original delayed-start gonadotropin-releasing hormone antagonist protocol using medroxyprogesterone acetate (MPA) and high-dose gonadotropin in patients with poor ovarian response.MethodsOverall, 156 patients with recurrent assisted reproductive technology failure who underwent the original protocol were included. They received cetrorelix acetate (3 mg) and MPA (10 mg) on cycle day 3, and high-dose gonadotropin was initiated on day 11. When the leading follicle reached 14 mm, ganirelix acetate (0.25 mg) was administered until the trigger day. The number of oocytes retrieved, metaphase II (MII) oocytes, two pronuclear (2PN) zygotes, and good blastocysts and live birth rates were compared between the previous (Cycle A) and original (Cycle B) cycles in three groups (Group A, all patients; Group B, poor responders; and Group C, patients with poor-quality embryos).ResultsIn Group A (n=156), the number of MII oocytes (3.6 ± 3.3 versus 4.5 ± 3.6), 2PN zygotes (2.8 ± 2.9 versus 3.8 ± 3.1), good blastocysts (0.5 ± 0.9 versus 1.2 ± 1.6), and live birth rates (0.6 versus 24.4) significantly increased in Cycle B. Similar results were obtained in Group B (n=83; 2PN zygotes [1.7 ± 1.7 versus 2.3 ± 1.8], good blastocysts [0.4 ± 0.7 versus 0.9 ± 1.3], live birth rates [0 versus 18.1]) and Group C (n=73; MII oocytes [5.1 ± 3.8 versus 6.6 ± 4.0], 2PN zygotes [4.0 ± 3.4 versus 5.4 ± 3.4], good blastocysts [0.7 ± 1.1 versus 1.6 ± 1.9], and live birth rates [1.4 versus 31.5]).ConclusionThis original protocol increased the number of MII oocytes retrieved, 2PN zygotes, good blastocysts, and live birth rates in both poor responders and in patients with poor-quality embryos

Correspondence between otolith microstructual changes and early life history events in Anguilla marmorata leptocephali and glass eels

To determine the exact correspondence between otolith characteristics and early life history events such as metamorphosis, coastal migration and recruitment to estuaries, both otolith microstructure and microchemistry analyses were applied to a sequential developmental series of samples, e. g. leptocephali, a metamorphosing larva, oceanic glass eels, and coastal glass eels. Total length and age were 10.1-50.7 mm and 22-137 d in leptocephali, 46.3 mm and 147 d in a metamorphosing larva, 47.8, 48.6 mm and 159, 160 d in oceanic glass eels, and 47.9-54.8 mm and 119-168 d in coastal glass eels. Checks at hatching and first feeding were observed in all specimens, but metamorphosis and freshwater checks were observed only in some specimens. It was confirmed that the abrupt drop in otolith Sr : Ca ratios and drastic increases of otolith increment widths in the metamorphosing larval stage correspond to the onset of metamorphosis, and the decrease after the peaks suggested the completion of metamorphosis, because the metamorphosing larva had no decrease in incremental widths. The relatively conserved Sr : Ca ratios decreased sharply in synchrony with the increasing increment widths. This study provides the first direct evidence that these drastic changes in otolith microstructure and microchemistry actually occur during metamorphosis, which has been only hypothesized

Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata)

Non-human primates are our closest relatives and are of special interest for ecological, evolutionary and biomedical research. The Japanese macaque (Macaca fuscata) has contributed to the progress of primatology and neurosciences over 60 years. Despite this importance, the molecular and cellular basis of the Japanese macaque remains unexplored since useful cellular tools are lacking. Here we generated induced pluripotent stem cells (iPSCs) from skin fibroblasts of the Japanese macaque with Sendai virus or plasmid vectors. The Japanese macaque iPSCs (jm-iPSCs) were established under feeder-free culture conditions, but feeder cells turned out to be essential for their maintenance. The jm-iPSCs formed human iPSC-like flat colonies which were positive for pluripotent antigens including alkaline phosphatase, SSEA4, and TRA-1-81. They also expressed endogenous OCT3/4, SOX2, L-MYC, and KLF4 and other pluripotent marker genes. The potential to differentiate into all three germ layers and neural stem cells was confirmed by embryoid body and neurosphere formation, respectively. The jm-iPSCs will provide a robust in vitro tool for investigating the underlying mechanisms of development and physiology studies with the Japanese macaque

Understanding Novel Superconductors with Ab Initio Calculations

This chapter gives an overview of the progress in the field of computational superconductivity. Following the MgB2 discovery (2001), there has been an impressive acceleration in the development of methods based on Density Functional Theory to compute the critical temperature and other physical properties of actual superconductors from first-principles. State-of-the-art ab-initio methods have reached predictive accuracy for conventional (phonon-mediated) superconductors, and substantial progress is being made also for unconventional superconductors. The aim of this chapter is to give an overview of the existing computational methods for superconductivity, and present selected examples of material discoveries that exemplify the main advancements.Comment: 38 pages, 10 figures, Contribution to Springer Handbook of Materials Modellin

Advances in the treatment of prolactinomas

Prolactinomas account for approximately 40% of all pituitary adenomas and are an important cause of hypogonadism and infertility. The ultimate goal of therapy for prolactinomas is restoration or achievement of eugonadism through the normalization of hyperprolactinemia and control of tumor mass. Medical therapy with dopamine agonists is highly effective in the majority of cases and represents the mainstay of therapy. Recent data indicating successful withdrawal of these agents in a subset of patients challenge the previously held concept that medical therapy is a lifelong requirement. Complicated situations, such as those encountered in resistance to dopamine agonists, pregnancy, and giant or malignant prolactinomas, may require multimodal therapy involving surgery, radiotherapy, or both. Progress in elucidating the mechanisms underlying the pathogenesis of prolactinomas may enable future development of novel molecular therapies for treatment-resistant cases. This review provides a critical analysis of the efficacy and safety of the various modes of therapy available for the treatment of patients with prolactinomas with an emphasis on challenging situations, a discussion of the data regarding withdrawal of medical therapy, and a foreshadowing of novel approaches to therapy that may become available in the future

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead