Morphofunctional peculiarities of tissue of oral cavity in chronic recurrent aphthous stomatitis with therapeutical correction

Chronic recurrent aphthous stomatitis (CRAS) belongs to the group of chronic, inflammatory, ulcerative diseases of the oral mucosa. The aim of this study was to identify the morphofunctional peculiarities in chronic recurrent aphthous stomatitis with therapeutical correction in soft tissues of the oral cavity of experimental animals in the modeling of chronic recurrent aphthous stomatitis. We performed experimental investigation for study of the morpho-functional state of tissues of the oral mucosa in CRAS and formed three groups of animals (rabbits) with different methods of treatment. Histological investigation have been performed. Conclusion of our research is that correction of tissual changes in chronic recurrent aphthous stomatitis could be obtained with application of gel with β-carotene, α-tocopherol, a mixture of vegetable oils; with ozone therapy and their combination

Glutathione transferase activity and reduce glutathione content in the cytosol of rat gastric mucosa cells under carcinogen N-methyl-N'-nitro-Nnitrosoguanidine treatment

Aim. To determine the activity of glutathione transferase (GT) and the content of reduced glutathione (GSH) in the cytosol of the gastric mucosa cells in experimental gastrocarcinogenesis. Methods. The activity of GT was determined spectrophotometrically, the content of GSH was measured spectrofluorimetrically. Gastrocarcinogenesis was initiated by 10-week replacement of drinking water by 0.01 % solution of carcinogen N-methyl-N'-nitro-N-nitrosoguanidine, at the same time the rats were given a diet containing 5 % NaCl. Results. It was established that at the end of the 4th and 6th weeks of consumption of carcino- gen and NaCl, the activity of GT increased by 26 and 94 %, whereas the content of GSH increased by 135 and 85 %, respectively. After 12 weeks there was a decrease in the activity of GT by 50 % and the maximum decrease in the GSH concentration by 69 %. At the end of the 18th and 24th weeks it was recorded the increase in the activity of GT by 44 and 47 % and the decrease in the GSH content by 55 and 52 %. Conclusions. The changes in the activity of GT and GSH-content are evidence of the violation of glutathione homeostasis, which may cause the delay as well as initiation of development of the pathology. The reduction of GSH is established at the early stages of tumors formation. Keywords: glutathione transferase, reduced glutathione, gastric cancer.Мета. Визначити глутатіонтрансферазну (ГТ) активність і вміст відновленого глутатіону (GSH) у цитозолі клітин слизової оболонки шлунка за умов експериментального гастроканцерогенезу. Методи. ГТ-активність визначали спектрофотометричним методом, вміст GSH – методом спектрофлуориметрії. Гастроканцерогенез ініціювали 10-тижневою заміною питної води на 0,01 %-й розчин канцеро- гену N-метил-N'-нітро-N-нітрозогуанідину з одночасним переведенням щурів на корм, який містить 5 % NaCl. Результати. По закінченні 4- і 6-тижневого споживання щурами канцерогену і NaCl ГТ-активність зростала відповідно на 26 і 94 %, тоді як вміст GSH – на 135 і 85 %. Через 12 тижнів спостерігали зниження активності ГТ на 50 % і максимальне зменшення концентрації GSH на 69 %. Після 18 і 24 тижнів зафіксовано зростання ГТ-активності на 44 і 47 % та зниження вмісту GSH на 55 і 52 %. Висновки. Зміни ГТ-активності і концентрації GSH свідчать про порушення глутатіонового гомеостазу, що може призводити як до затримки, так і ініціації розвитку патології. Встановлено зниження вмісту GSH на ранніх стадіях формування пухлин. Ключові слова: глутатіонтрансфераза, відновлений глутатіон, рак шлунка.Цель. Определить глутатионтрансферазную (ГТ) активность и содержание восстановленного глутатиона (GSH) в цитозоле клеток слизистой оболочки желудка в условиях экспериментального гастроканцерогенеза. Методы. ГТ-активность определяли спектрофотометрическим методом, содержание GSH – методом спектрофлуориметрии. Гастроканцерогенез инициировали 10-недельной заменой питьевой воды на 0,01 %-й раствор канцерогена N-метил-N'-нитро-N-нитрозогуанидина с одновременным переводом крыс на корм, содержащий 5 % NaCl. Результаты. По окончании 4 и 6 недель потребления крысами канцерогена и NaCl ГТ-активность увеличилась соответственно на 26 и 94 %, тогда как содержание GSH – на 135 и 85 %. Через 12 недель наблюдали снижение ГТ-активности на 50 % и максимальное уменьшение концентрации GSH на 69 %. Спустя 18 и 24 недели зафиксировано возрастание активности ГТ на 44 и 47 %, а также снижение содержания GSH на 55 и 52 %. Выводы. Изменения ГТ- активности и концентрации GSH свидетельствуют о нарушении гомеостаза глутатиона, что может вызывать как задержку, так и инициацию развития патологии. Установлено снижение содержания GSH на ранних стадиях формирования опухолей. Ключевые слова: глутатионтрансфераза, восстановленный глутатион, рак желудка

The phospho-c-JUN (Ser63) content in mucosa cells under experimental development of gastric cancer

Aim: The aim of the study was to determine the phosphorylated c-Jun content and reduced and oxidized glutathione (GSH/GSSG) ratio in gastric mucosa cells during the process of gastric cancer development in rats. Materials and Methods: Gastric carcinoge­nesis was initiated in 80 white male rats by 10-week replacement of drinking water with 0.01% solution of N-methyl-N´-nitro-N-nitrosoguanidine, at the same time they were redefined on diet containing 5% NaCl. Then the animals were fed with standard vivarium diet till the end of 24th week. The gastric mucosa cells were examined at the end of 4th, 6th, 8th, 10th, 12th, 18th, and 24th weeks. Sandwich ELISA method was used to determine the content of phospho-c-Jun. The contents of GSH and GSSG were analyzed by spectrofluorymetric method with the use of orthophthalic aldehyde. Results: At the end of 6th, 8th, 10th weeks of MNNG and NaCl treatment the gastric mucosa cells were characterized by 4-, 6.3-, 1.9-fold higher content of phospho-c-Jun compared to the control, respectively, and 12, 18 and 24 weeks there was registered a stable increase of phospho-c-Jun content on the average at 3.6-fold compared to control values. Starting from 6th week of gastric cancer development an average decrease of GSH/GSSG was 3.4-fold compared with the control. Conclusion: During gastric carcinogenesis there was registered the decrease of GSH/GSSG ratio and increased level of phosphorylated c-Jun what points on MAP-kinase cascade activation in prooxidant conditions. Key Words: c-Jun, reduce glutathione (GSH), oxidized glutathione (GSSG), gastric cancer

Measurement of the View the tt production cross-section using eμ events with b-tagged jets in pp collisions at √s = 13 TeV with the ATLAS detector

This paper describes a measurement of the inclusive top quark pair production cross-section (σtt¯) with a data sample of 3.2 fb−1 of proton–proton collisions at a centre-of-mass energy of √s = 13 TeV, collected in 2015 by the ATLAS detector at the LHC. This measurement uses events with an opposite-charge electron–muon pair in the final state. Jets containing b-quarks are tagged using an algorithm based on track impact parameters and reconstructed secondary vertices. The numbers of events with exactly one and exactly two b-tagged jets are counted and used to determine simultaneously σtt¯ and the efficiency to reconstruct and b-tag a jet from a top quark decay, thereby minimising the associated systematic uncertainties. The cross-section is measured to be: σtt¯ = 818 ± 8 (stat) ± 27 (syst) ± 19 (lumi) ± 12 (beam) pb, where the four uncertainties arise from data statistics, experimental and theoretical systematic effects, the integrated luminosity and the LHC beam energy, giving a total relative uncertainty of 4.4%. The result is consistent with theoretical QCD calculations at next-to-next-to-leading order. A fiducial measurement corresponding to the experimental acceptance of the leptons is also presented

Search for TeV-scale gravity signatures in high-mass final states with leptons and jets with the ATLAS detector at sqrt [ s ] = 13TeV

A search for physics beyond the Standard Model, in final states with at least one high transverse momentum charged lepton (electron or muon) and two additional high transverse momentum leptons or jets, is performed using 3.2 fb−1 of proton–proton collision data recorded by the ATLAS detector at the Large Hadron Collider in 2015 at √s = 13 TeV. The upper end of the distribution of the scalar sum of the transverse momenta of leptons and jets is sensitive to the production of high-mass objects. No excess of events beyond Standard Model predictions is observed. Exclusion limits are set for models of microscopic black holes with two to six extra dimensions

The performance of the jet trigger for the ATLAS detector during 2011 data taking

The performance of the jet trigger for the ATLAS detector at the LHC during the 2011 data taking period is described. During 2011 the LHC provided proton–proton collisions with a centre-of-mass energy of 7 TeV and heavy ion collisions with a 2.76 TeV per nucleon–nucleon collision energy. The ATLAS trigger is a three level system designed to reduce the rate of events from the 40 MHz nominal maximum bunch crossing rate to the approximate 400 Hz which can be written to offline storage. The ATLAS jet trigger is the primary means for the online selection of events containing jets. Events are accepted by the trigger if they contain one or more jets above some transverse energy threshold. During 2011 data taking the jet trigger was fully efficient for jets with transverse energy above 25 GeV for triggers seeded randomly at Level 1. For triggers which require a jet to be identified at each of the three trigger levels, full efficiency is reached for offline jets with transverse energy above 60 GeV. Jets reconstructed in the final trigger level and corresponding to offline jets with transverse energy greater than 60 GeV, are reconstructed with a resolution in transverse energy with respect to offline jets, of better than 4 % in the central region and better than 2.5 % in the forward direction

Search for dark matter produced in association with a hadronically decaying vector boson in pp collisions at sqrt (s) = 13 TeV with the ATLAS detector

A search is presented for dark matter produced in association with a hadronically decaying W or Z boson using 3.2 fb−1 of pp collisions at View the MathML sources=13 TeV recorded by the ATLAS detector at the Large Hadron Collider. Events with a hadronic jet compatible with a W or Z boson and with large missing transverse momentum are analysed. The data are consistent with the Standard Model predictions and are interpreted in terms of both an effective field theory and a simplified model containing dark matter

Search for resonances in the mass distribution of jet pairs with one or two jets identified as b-jets in proton–proton collisions at √s=13TeV with the ATLAS detector

Searches for high-mass resonances in the dijet invariant mass spectrum with one or two jets identi-fied as b-jets are performed using an integrated luminosity of 3.2fb−1of proton–proton collisions with a centre-of-mass energy of √s=13TeVrecorded by the ATLAS detector at the Large Hadron Collider. Noevidence of anomalous phenomena is observed in the data, which are used to exclude, at 95%credibility level, excited b∗quarks with masses from 1.1TeVto 2.1TeVand leptophobic Z bosons with masses from 1.1TeVto 1.5TeV. Contributions of a Gaussian signal shape with effective cross sections ranging from approximately 0.4 to 0.001pb are also excluded in the mass range 1.5–5.0TeV

Measurement of the double-differential high-mass Drell-Yan cross section in pp collisions at √s = 8 TeV with the ATLAS detector

This paper presents a measurement of the double-differential cross section for the Drell-Yan Z/γ∗ → ℓ+ℓ− and photon-induced γγ → ℓ+ℓ− processes where ℓ is an electron or muon. The measurement is performed for invariant masses of the lepton pairs, mℓℓ, between 116 GeV and 1500 GeV using a sample of 20.3 fb−1 of pp collisions data at centre-of-mass energy of √s = 8 TeV collected by the ATLAS detector at the LHC in 2012. The data are presented double differentially in invariant mass and absolute dilepton rapidity as well as in invariant mass and absolute pseudorapidity separation of the lepton pair. The single-differential cross section as a function of mℓℓ is also reported. The electron and muon channel measurements are combined and a total experimental precision of better than 1% is achieved at low mℓℓ. A comparison to next-to-next-to-leading order perturbative QCD predictions using several recent parton distribution functions and including next-to-leading order electroweak effects indicates the potential of the data to constrain parton distribution functions. In particular, a large impact of the data on the photon PDF is demonstrated

Charged-particle distributions in √s=13 TeV pp interactions measured with the ATLAS detector at the LHC

Charged-particle distributions are measured in proton–proton collisions at a centre-of-mass energy of 13 TeV, using a data sample of nearly 9 million events, corresponding to an integrated luminosity of 170 μb−1170 μb−1, recorded by the ATLAS detector during a special Large Hadron Collider fill. The charged-particle multiplicity, its dependence on transverse momentum and pseudorapidity and the dependence of the mean transverse momentum on the charged-particle multiplicity are presented. The measurements are performed with charged particles with transverse momentum greater than 500 MeV and absolute pseudorapidity less than 2.5, in events with at least one charged particle satisfying these kinematic requirements. Additional measurements in a reduced phase space with absolute pseudorapidity less than 0.8 are also presented, in order to compare with other experiments. The results are corrected for detector effects, presented as particle-level distributions and are compared to the predictions of various Monte Carlo event generators