Diffraction-limited superresolution ptychography in the Rayleigh-Sommerfeld regime

Superresolution in lensless near field ptychography is demonstrated via the application of a strongly curved illumination function. The reconstruction is performed using the Rayleigh-Sommerfeld diffraction integral, which is implemented via a pixel-size adjustable angular spectrum method. In this manner the reconstructed object details, which are not only smaller than the pixel-size of the sensor but even smaller than the smallest resolvable object detail defined by the effective NA of the 2D sensor, is enabled. The expected resolution, as predicted by the angles of scatter present in the optical configuration, is experimentally validated using a US air force resolution test target. The approach discussed here is not only limited to ptychography, but can be extended to other coherent diffractive imaging modalities such as object scanning holography or optical diffraction tomography, so as to enable high resolution near field quantitative phase imaging

Modal decomposition of a propagating matter wave via electron ptychography

We employ ptychography, a phase-retrieval imaging technique, to show experimentally for the first time that a partially coherent high-energy matter (electron) wave emanating from an extended source can be decomposed into a set of mutually independent modes of minimal rank. Partial coherence significantly determines the optical transfer properties of an electron microscope and so there has been much work on this subject. However, previous studies have employed forms of interferometry to determine spatial coherence between discrete points in the wavefield. Here we use the density matrix to derive a formal quantum mechanical description of electron ptychography and use it to measure a full description of the spatial coherence of a propagating matter wavefield, at least to the within the fundamental uncertainties of the measurements we can obtain

Image feature delocalization in defocused probe electron ptychography

Electron ptychography can in principle convert a conventional scanning electron microscope (SEM) into a good quality transmission electron microscope (TEM). An improvement in resolution of about a factor of 5 over the lens-defined resolution of an SEM was first demonstrated in 2012 by Humphry et.al. However, the results from that work showed some delocalization in the atomic fringes of the gold particles used as a test specimen for the technique. Here we explore factors that result in the delocalization effect when a defocused probe is used for the ptychographic data collection: source incoherence, the effects of detector faults, data truncation and a poorly calibrated illumination step size (or camera length). Various mitigation strategies are tested, including modal decomposition of the incoherence in the beam. We reprocess the data from the original SEM experiment to show that these refinements significantly improve the reconstruction

Sydney Conservatorium of Music Postgraduate Handbook 2009

In stage III breast carcinoma, metastasized disease needs to be determined. In the past, conventional imaging by liver ultrasound, chest X-ray and bone scintigraphy was the work-up of choice. Recently, FDG-PET/CT was found to have additional value, but clinicians are hesitant to introduce this technique. We present three patients in whom FDG-PET/CT was applied. A 61-year-old woman with stage III breast carcinoma after conventional work-up was upstaged to stage IV breast carcinoma by FDG-PET/CT, upon which her treatment was changed. A 55-year-old woman suspected of stage IV breast carcinoma after conventional imaging was downstaged to stage III after FDG-PET/CT. Her treatment was changed as well. In a 78-year-old woman with recurrent breast carcinoma, the diagnostic certainty of stage III breast carcinoma was increased by FDG-PET/CT. We conclude that FDG-PET/CT is valuable for adequately diagnosing metastases in patients with stage III breast carcinoma and can replace conventional imaging

CYP1A2 and coffee intake and the modifying effect of sex, age, and smoking

Background: The enzyme CYP1A2 (cytochrome 1A2) is involved in the metabolism of certain drugs and caffeine, and its activity can be influenced by factors such as sex, age, and smoking. The single nucleotide polymorphism (SNP) rs762551A>C, which has also been studied for its modifying effect on cardiovascular disease, has been reported to alter enzyme activity. Objective: The objective was to study the effect of CYP1A2, sex, age, and smoking on coffee intake. Design: Within the Rotterdam Study, a population-based cohort, all coffee drinkers for whom genome-wide association data were available were selected. Because SNP rs762551 was not on the Illumina 550 platform, SNP rs2472299 was used as a proxy, with the A allele of rs762551 linked to the G allele of rs2472299. Linear regression analyses were used to determine the effect and interaction of rs2472299, sex, age, and smoking on coffee intake. Adjusted geometric means of coffee intake were calculated per genotype for the different smoking and sex strata by using multivariable general linear models. A combined analysis, with the use of a "risk score,"was performed to determine the contribution of each separate factor. Results: rs2472299G>A, female sex, and nonsmoking were significantly inversely related to coffee intake. Coffee intake was lowest in nonsmoking women homozygous for rs2472299G>A (3.49 cups/d; ∼436 mL). All factors contributed almost linearly to the intake of coffee, with the highest coffee intake in smoking men without the A allele (5.32 cups/d; ∼665 mL). Conclusion: rs2472299G>A, linked to rs762551A>C, sex, age, and smoking significantly contribute to coffee intake

Efficient large field of view electron phase imaging using near-field electron ptychography with a diffuser

Most implementations of ptychography on the electron microscope operate in scanning transmission (STEM) mode, where a small focussed probe beam is rapidly scanned across the sample. In this paper we introduce a different approach based on near-field ptychography, where the focussed beam is replaced by a wide-field, structured illumination, realised through a purpose-designed etched Silicon Nitride window. We show that fields of view as large as 100 μm2 can be imaged using the new approach, and that quantitative electron phase images can be reconstructed from as few as nine near-field diffraction pattern measurements

Status quo and challenges of rice production in sub-Saharan Africa

Rice production in sub-Saharan Africa (SSA) has increaed ten-fold since 1961, whereas its consumption has exceeded the production and the regional self-sufficiency rate is only 48% in 2020. Increase in rice production has come mainly from increased harvested area. Yield increase has been limited and the current average yield in SSA is around 2 t ha−1. This paper aims to provide the status quo of (i) current rice production and its challenges, (ii) selected achievements in rice agronomy research mainly by the Africa Rice Center and its partners, and (iii) perspectives for future research on rice agronomy in SSA. The major problems confronting rice production include low yield in rainfed environments, accounting for 70% of the total rice harvested area. Rainfed rice yields are strongly affected by climate extremes such as water stresses, soil-related constraints, and sub-optimum natural resource management and crop management practices by smallholder farmers including poor water management, and suboptimal use of fertilizers, herbicides, and machineries. For alleviating these constraints, a wide range of technologies have been developed and introduced over the last three decades. These include water conservation technologies in rainfed and irrigated lowland rice, site-specific nutrient management practices, decision support tools such as crop growth simulation models, and labor-saving technologies. We conclude that further research efforts are needed to develop locally adapted agronomic solutions for sustainable intensification, especially in rainfed rice to enhance the resilience to climate change and increase land and labor productivity and sustainability of rice cultivation in SSA

An assessment of the resolution limitation due to radiation-damage in x-ray diffraction microscopy

X-ray diffraction microscopy (XDM) is a new form of x-ray imaging that is being practiced at several third-generation synchrotron-radiation x-ray facilities. Although only five years have elapsed since the technique was first introduced, it has made rapid progress in demonstrating high-resolution threedimensional imaging and promises few-nm resolution with much larger samples than can be imaged in the transmission electron microscope. Both life- and materials-science applications of XDM are intended, and it is expected that the principal limitation to resolution will be radiation damage for life science and the coherent power of available x-ray sources for material science. In this paper we address the question of the role of radiation damage. We use a statistical analysis based on the so-called "dose fractionation theorem" of Hegerl and Hoppe to calculate the dose needed to make an image of a lifescience sample by XDM with a given resolution. We conclude that the needed dose scales with the inverse fourth power of the resolution and present experimental evidence to support this finding. To determine the maximum tolerable dose we have assembled a number of data taken from the literature plus some measurements of our own which cover ranges of resolution that are not well covered by reports in the literature. The tentative conclusion of this study is that XDM should be able to image frozen-hydrated protein samples at a resolution of about 10 nm with "Rose-criterion" image quality.Comment: 9 pages, 4 figure

Search for the standard model Higgs boson decaying into two photons in pp collisions at sqrt(s)=7 TeV

A search for a Higgs boson decaying into two photons is described. The analysis is performed using a dataset recorded by the CMS experiment at the LHC from pp collisions at a centre-of-mass energy of 7 TeV, which corresponds to an integrated luminosity of 4.8 inverse femtobarns. Limits are set on the cross section of the standard model Higgs boson decaying to two photons. The expected exclusion limit at 95% confidence level is between 1.4 and 2.4 times the standard model cross section in the mass range between 110 and 150 GeV. The analysis of the data excludes, at 95% confidence level, the standard model Higgs boson decaying into two photons in the mass range 128 to 132 GeV. The largest excess of events above the expected standard model background is observed for a Higgs boson mass hypothesis of 124 GeV with a local significance of 3.1 sigma. The global significance of observing an excess with a local significance greater than 3.1 sigma anywhere in the search range 110-150 GeV is estimated to be 1.8 sigma. More data are required to ascertain the origin of this excess.Comment: Submitted to Physics Letters

Search for a W' boson decaying to a bottom quark and a top quark in pp collisions at sqrt(s) = 7 TeV

Results are presented from a search for a W' boson using a dataset corresponding to 5.0 inverse femtobarns of integrated luminosity collected during 2011 by the CMS experiment at the LHC in pp collisions at sqrt(s)=7 TeV. The W' boson is modeled as a heavy W boson, but different scenarios for the couplings to fermions are considered, involving both left-handed and right-handed chiral projections of the fermions, as well as an arbitrary mixture of the two. The search is performed in the decay channel W' to t b, leading to a final state signature with a single lepton (e, mu), missing transverse energy, and jets, at least one of which is tagged as a b-jet. A W' boson that couples to fermions with the same coupling constant as the W, but to the right-handed rather than left-handed chiral projections, is excluded for masses below 1.85 TeV at the 95% confidence level. For the first time using LHC data, constraints on the W' gauge coupling for a set of left- and right-handed coupling combinations have been placed. These results represent a significant improvement over previously published limits.Comment: Submitted to Physics Letters B. Replaced with version publishe