Coalescing binary systems of compact objects: Dynamics of angular momenta

The end state of a coalescing binary of compact objects depends strongly on the final total mass M and angular momentum J. Since gravitational radiation emission causes a slow evolution of the binary system through quasi-circular orbits down to the innermost stable one, in this paper we examine the corresponding behavior of the ratio J/M^2 which must be less than 1(G/c) or about 0.7(G/c) for the formation of a black hole or a neutron star respectively. The results show cases for which, at the end of the inspiral phase, the conditions for black hole or neutron star formation are not satisfied. The inclusion of spin effects leads us to a study of precession equations valid also for the calculation of gravitational waveforms.Comment: 22 pages, AASTeX and 13 figures in PostScrip

Evaluation of biocides in oilfield environments using fluorescent <i>in-situ</i> hybridization

Microbiologically influenced corrosion and souring of oilfield reservoirs are process frequently provoked by the sulphate-reducing bacteria. The most common method applied in the industry for preventing or controlling the deleterious effect caused by the presence of microorganisms is the addition of chemical agents (biocides) aimed at killing the microorganisms or inhibiting the microbial growth. Traditionally, biocide selection and testing are based on NACE standard TM0194 which implies the use of culturing for enumerating the bacteria surviving the treatment. To overcome culturing limitations, we used Fluorescent in-situ Hybridization to assist in the evaluation of biocides applied in water production treatment plants. Biocides were based on THPS (40% (B1); 75% (B2)) and 40% of a mixture (1/1, v/v) of THPS and benzalkonium chloride (B3) applied at two concentrations: 50 and 400 mg/L. The relation between the number of cells visualized with the fluorescent probes Eub338 and SRB385 (for eubacteria and SRB populations respectively) and the DAPI-stained cells (PR%) was used as an indication of the biocide efficiency. B1 and B3 gave a high PR% indicating that the chemical induced the metabolic cell activity. Only the highest B2 concentration showed effectiveness on eubacteria and SRB populations. Thus, through the application of FISH we were able to distinguish concentration effects of the THPS, discriminating sublethal from net inhibitory effects. The possibility of including FISH into the protocols for the control of the biocides in water treatment plants could improve the biocide selection and the adjustment of their concentration in order to maintain the water system with a low density of metabolically active cells. This would avoid the misuse of chemicals with their consequent economic and ecological impacts.Centro de Investigación y Desarrollo en Fermentaciones IndustrialesCentro de Investigación y Desarrollo en Tecnología de Pintura

Evaluation of biocides in oilfield environments using fluorescent <i>in-situ</i> hybridization

Microbiologically influenced corrosion and souring of oilfield reservoirs are process frequently provoked by the sulphate-reducing bacteria. The most common method applied in the industry for preventing or controlling the deleterious effect caused by the presence of microorganisms is the addition of chemical agents (biocides) aimed at killing the microorganisms or inhibiting the microbial growth. Traditionally, biocide selection and testing are based on NACE standard TM0194 which implies the use of culturing for enumerating the bacteria surviving the treatment. To overcome culturing limitations, we used Fluorescent in-situ Hybridization to assist in the evaluation of biocides applied in water production treatment plants. Biocides were based on THPS (40% (B1); 75% (B2)) and 40% of a mixture (1/1, v/v) of THPS and benzalkonium chloride (B3) applied at two concentrations: 50 and 400 mg/L. The relation between the number of cells visualized with the fluorescent probes Eub338 and SRB385 (for eubacteria and SRB populations respectively) and the DAPI-stained cells (PR%) was used as an indication of the biocide efficiency. B1 and B3 gave a high PR% indicating that the chemical induced the metabolic cell activity. Only the highest B2 concentration showed effectiveness on eubacteria and SRB populations. Thus, through the application of FISH we were able to distinguish concentration effects of the THPS, discriminating sublethal from net inhibitory effects. The possibility of including FISH into the protocols for the control of the biocides in water treatment plants could improve the biocide selection and the adjustment of their concentration in order to maintain the water system with a low density of metabolically active cells. This would avoid the misuse of chemicals with their consequent economic and ecological impacts.Centro de Investigación y Desarrollo en Fermentaciones IndustrialesCentro de Investigación y Desarrollo en Tecnología de Pintura

EEG Fractal Analysis Reflects Brain Impairment after Stroke

Stroke is the commonest cause of disability. Novel treatments require an improved understanding of the underlying mechanisms of recovery. Fractal approaches have demonstrated that a single metric can describe the complexity of seemingly random fluctuations of physiological signals. We hypothesize that fractal algorithms applied to electroencephalographic (EEG) signals may track brain impairment after stroke. Sixteen stroke survivors were studied in the hyperacute (<48 h) and in the acute phase (∼1 week after stroke), and 35 stroke survivors during the early subacute phase (from 8 days to 32 days and after ∼2 months after stroke): We compared resting-state EEG fractal changes using fractal measures (i.e., Higuchi Index, Tortuosity) with 11 healthy controls. Both Higuchi index and Tortuosity values were significantly lower after a stroke throughout the acute and early subacute stage compared to healthy subjects, reflecting a brain activity which is significantly less complex. These indices may be promising metrics to track behavioral changes in the very early stage after stroke. Our findings might contribute to the neurorehabilitation quest in identifying reliable biomarkers for a better tailoring of rehabilitation pathways

Mechanisms of endothelial cell dysfunction in cystic fibrosis

Although cystic fibrosis (CF) patients exhibit signs of endothelial perturbation, the functions of the cystic fibrosis conductance regulator (CFTR) in vascular endothelial cells (EC) are poorly defined. We sought to uncover biological activities of endothelial CFTR, relevant for vascular homeostasis and inflammation. We examined cells from human umbilical cords (HUVEC) and pulmonary artery isolated from non-cystic fibrosis (PAEC) and CF human lungs (CF-PAEC), under static conditions or physiological shear. CFTR activity, clearly detected in HUVEC and PAEC, was markedly reduced in CF-PAEC. CFTR blockade increased endothelial permeability to macromolecules and reduced trans‑endothelial electrical resistance (TEER). Consistent with this, CF-PAEC displayed lower TEER compared to PAEC. Under shear, CFTR blockade reduced VE-cadherin and p120 catenin membrane expression and triggered the formation of paxillin- and vinculin-enriched membrane blebs that evolved in shrinking of the cell body and disruption of cell-cell contacts. These changes were accompanied by enhanced release of microvesicles, which displayed reduced capability to stimulate proliferation in recipient EC. CFTR blockade also suppressed insulin-induced NO generation by EC, likely by inhibiting eNOS and AKT phosphorylation, whereas it enhanced IL-8 release. Remarkably, phosphodiesterase inhibitors in combination with a β2 adrenergic receptor agonist corrected functional and morphological changes triggered by CFTR dysfunction in EC. Our results uncover regulatory functions of CFTR in EC, suggesting a physiological role of CFTR in the maintenance EC homeostasis and its involvement in pathogenetic aspects of CF. Moreover, our findings open avenues for novel pharmacology to control endothelial dysfunction and its consequences in CF

<i>Gaia</i> Data Release 1. Summary of the astrometric, photometric, and survey properties

Context. At about 1000 days after the launch of Gaia we present the first Gaia data release, Gaia DR1, consisting of astrometry and photometry for over 1 billion sources brighter than magnitude 20.7. Aims. A summary of Gaia DR1 is presented along with illustrations of the scientific quality of the data, followed by a discussion of the limitations due to the preliminary nature of this release. Methods. The raw data collected by Gaia during the first 14 months of the mission have been processed by the Gaia Data Processing and Analysis Consortium (DPAC) and turned into an astrometric and photometric catalogue. Results. Gaia DR1 consists of three components: a primary astrometric data set which contains the positions, parallaxes, and mean proper motions for about 2 million of the brightest stars in common with the HIPPARCOS and Tycho-2 catalogues – a realisation of the Tycho-Gaia Astrometric Solution (TGAS) – and a secondary astrometric data set containing the positions for an additional 1.1 billion sources. The second component is the photometric data set, consisting of mean G-band magnitudes for all sources. The G-band light curves and the characteristics of ∼3000 Cepheid and RR-Lyrae stars, observed at high cadence around the south ecliptic pole, form the third component. For the primary astrometric data set the typical uncertainty is about 0.3 mas for the positions and parallaxes, and about 1 mas yr−1 for the proper motions. A systematic component of ∼0.3 mas should be added to the parallax uncertainties. For the subset of ∼94 000 HIPPARCOS stars in the primary data set, the proper motions are much more precise at about 0.06 mas yr−1. For the secondary astrometric data set, the typical uncertainty of the positions is ∼10 mas. The median uncertainties on the mean G-band magnitudes range from the mmag level to ∼0.03 mag over the magnitude range 5 to 20.7. Conclusions. Gaia DR1 is an important milestone ahead of the next Gaia data release, which will feature five-parameter astrometry for all sources. Extensive validation shows that Gaia DR1 represents a major advance in the mapping of the heavens and the availability of basic stellar data that underpin observational astrophysics. Nevertheless, the very preliminary nature of this first Gaia data release does lead to a number of important limitations to the data quality which should be carefully considered before drawing conclusions from the data

Consensus statement of the Italian society of pediatric allergy and immunology for the pragmatic management of children and adolescents with allergic or immunological diseases during the COVID-19 pandemic

The COVID-19 pandemic has surprised the entire population. The world has had to face an unprecedented pandemic. Only, Spanish flu had similar disastrous consequences. As a result, drastic measures (lockdown) have been adopted worldwide. Healthcare service has been overwhelmed by the extraordinary influx of patients, often requiring high intensity of care. Mortality has been associated with severe comorbidities, including chronic diseases. Patients with frailty were, therefore, the victim of the SARS-COV-2 infection. Allergy and asthma are the most prevalent chronic disorders in children and adolescents, so they need careful attention and, if necessary, an adaptation of their regular treatment plans. Fortunately, at present, young people are less suffering from COVID-19, both as incidence and severity. However, any age, including infancy, could be affected by the pandemic. Based on this background, the Italian Society of Pediatric Allergy and Immunology has felt it necessary to provide a Consensus Statement. This expert panel consensus document offers a rationale to help guide decision-making in the management of children and adolescents with allergic or immunologic diseases

The Variant rs1867277 in FOXE1 Gene Confers Thyroid Cancer Susceptibility through the Recruitment of USF1/USF2 Transcription Factors

In order to identify genetic factors related to thyroid cancer susceptibility, we adopted a candidate gene approach. We studied tag- and putative functional SNPs in genes involved in thyroid cell differentiation and proliferation, and in genes found to be differentially expressed in thyroid carcinoma. A total of 768 SNPs in 97 genes were genotyped in a Spanish series of 615 cases and 525 controls, the former comprising the largest collection of patients with this pathology from a single population studied to date. SNPs in an LD block spanning the entire FOXE1 gene showed the strongest evidence of association with papillary thyroid carcinoma susceptibility. This association was validated in a second stage of the study that included an independent Italian series of 482 patients and 532 controls. The strongest association results were observed for rs1867277 (OR[per-allele] = 1.49; 95%CI = 1.30–1.70; P = 5.9×10−9). Functional assays of rs1867277 (NM_004473.3:c.−283G>A) within the FOXE1 5′ UTR suggested that this variant affects FOXE1 transcription. DNA-binding assays demonstrated that, exclusively, the sequence containing the A allele recruited the USF1/USF2 transcription factors, while both alleles formed a complex in which DREAM/CREB/αCREM participated. Transfection studies showed an allele-dependent transcriptional regulation of FOXE1. We propose a FOXE1 regulation model dependent on the rs1867277 genotype, indicating that this SNP is a causal variant in thyroid cancer susceptibility. Our results constitute the first functional explanation for an association identified by a GWAS and thereby elucidate a mechanism of thyroid cancer susceptibility. They also attest to the efficacy of candidate gene approaches in the GWAS era

Identification of ORC1/CDC6-Interacting Factors in Trypanosoma brucei Reveals Critical Features of Origin Recognition Complex Architecture

DNA Replication initiates by formation of a pre-replication complex on sequences termed origins. In eukaryotes, the pre-replication complex is composed of the Origin Recognition Complex (ORC), Cdc6 and the MCM replicative helicase in conjunction with Cdt1. Eukaryotic ORC is considered to be composed of six subunits, named Orc1–6, and monomeric Cdc6 is closely related in sequence to Orc1. However, ORC has been little explored in protists, and only a single ORC protein, related to both Orc1 and Cdc6, has been shown to act in DNA replication in Trypanosoma brucei. Here we identify three highly diverged putative T. brucei ORC components that interact with ORC1/CDC6 and contribute to cell division. Two of these factors are so diverged that we cannot determine if they are eukaryotic ORC subunit orthologues, or are parasite-specific replication factors. The other we show to be a highly diverged Orc4 orthologue, demonstrating that this is one of the most widely conserved ORC subunits in protists and revealing it to be a key element of eukaryotic ORC architecture. Additionally, we have examined interactions amongst the T. brucei MCM subunits and show that this has the conventional eukaryotic heterohexameric structure, suggesting that divergence in the T. brucei replication machinery is limited to the earliest steps in origin licensing