Estimating the Net Energy Value of Expelled, Extruded Soybean Meal When Fed to Nursery Pigs

Solvent-extracted soybean meal (SSBM) is typically used as the primary protein source in swine diets in the United States. Mechanically extracted or expelled soybean meal (MSBM) typically has increased concentrations of fat and decreased concentrations of essential AA compared to SSBM, but MSBM has been demonstrated to have improved AA digestibility. This study aimed to estimate the NE value of MSBM relative to SSBM and to determine its effects on growth performance of late nursery pigs. A total of 297 pigs (241 × 600, DNA) were weaned (BW 11.2 lb) and placed into 60 pens (2 rooms of 30 pens) with 5 pigs per pen balanced by gender and weaning weight. Pigs were fed a common diet for 21 days. Then, pens of pigs (BW 20.7 lb) were randomly assigned to one of five treatments to provide 12 replications per treatment. Treatments consisted of increasing amounts of MSBM replacing SSBM in the diet (0, 25, 50, 75, and 100%). All diets were fed for 28 days and were formulated to 1.30% standardized ileal digestible lysine, and met or exceeded NRC2 recommendations for Lys:AA, Ca, and P. The SSBM has an NRC2 NE value of 946 kcal/lb and the 0% MSBM diet was formulated to 1091 kcal/lb and NE was not balanced between diets. Analyzed values for CP, ether extract, crude fiber, and total Lys for the SSBM were 47.28%, 0.47%, 3.80%, and 3.00%, respectively, while the MSBM contained 47.41%, 6.88%, 5.32%, and 2.99% respectively. The MSBM had increased values for KOH solubility and trypsin inhibitor (83.62 and 7026 TIU/g, respectively) compared to the SSBM (73.05 and 3011 TIU/g, respectively) while urease activity was similar between the two (0.03 vs. 0.02 Δ pH, respectively). Data were analyzed using PROC GLIMMIX (SAS v. 9.4) with pen as the experimental unit and room as the blocking factor. There was no evidence of differences in ADG and ADFI in pigs fed diets with increasing concentrations of MSBM. Pigs fed diets with increasing concentrations of MSBM had improved (linear, P \u3c 0.001) F/G and caloric efficiency on an NE basis. In conclusion, using caloric efficiency to estimate NE of the MSBM relative to SSBM, MSBM was estimated to have a value of 123% of SSBM NE or 1,164 kcal/lb. This increase in NE resulted in improved feed efficiency of nursery pigs

RAHI-SATHI Indo-U.S. Collaboration: The Evolution of a Trainee-Led Twinning Model in Global Health Into a Multidisciplinary Collaborative Program

BACKGROUND: In recent years there has been a surge in the number of global health programs operated by academic institutions. However, most of the existing programs describe partnerships that are primarily faculty-driven and supported by extramural funding. PROGRAM DESCRIPTION: Research and Advocacy for Health in India (RAHI, or pathfinder in Hindi) and Support and Action Towards Health-Equity in India (SATHI, or partnership in Hindi) are 2 interconnected, collaborative efforts between the University of Massachusetts Medical School (UMMS) and Charutar Arogya Mandal (CAM), a medical college and a tertiary care center in rural western India. The RAHI-SATHI program is the culmination of a series of student/trainee-led research and capacity strengthening initiatives that received institutional support in the form of faculty mentorship and seed funding. RAHI-SATHI\u27s trainee-led twinning approach overcomes traditional barriers faced by global health programs. Trainees help mitigate geographical barriers by acting as a bridge between members from different institutions, garner cultural insight through their ability to immerse themselves in a community, and overcome expertise limitations through pre-planned structured mentorship from faculty of both institutions. Trainees play a central role in cultivating trust among the team members and, in the process, they acquire personal leadership skills that may benefit them in their future careers. CONCLUSION: This paradigm of trainee-led twinning partnership promotes sustainability in an uncertain funding climate and provides a roadmap for conducting foundational work that is essential for the development of a broad, university-wide global health program

How To Perform Meaningful Estimates of Genetic Effects

Although the genotype-phenotype map plays a central role both in Quantitative and Evolutionary Genetics, the formalization of a completely general and satisfactory model of genetic effects, particularly accounting for epistasis, remains a theoretical challenge. Here, we use a two-locus genetic system in simulated populations with epistasis to show the convenience of using a recently developed model, NOIA, to perform estimates of genetic effects and the decomposition of the genetic variance that are orthogonal even under deviations from the Hardy-Weinberg proportions. We develop the theory for how to use this model in interval mapping of quantitative trait loci using Halley-Knott regressions, and we analyze a real data set to illustrate the advantage of using this approach in practice. In this example, we show that departures from the Hardy-Weinberg proportions that are expected by sampling alone substantially alter the orthogonal estimates of genetic effects when other statistical models, like F2 or G2A, are used instead of NOIA. Finally, for the first time from real data, we provide estimates of functional genetic effects as sets of effects of natural allele substitutions in a particular genotype, which enriches the debate on the interpretation of genetic effects as implemented both in functional and in statistical models. We also discuss further implementations leading to a completely general genotype-phenotype map

Quantification of Semi-Truck Cab Decontamination

Evidence suggests that the inside of vehicle cabs used for feed delivery may serve as a potential source for disease, yet there are no standardized protocols or scientific evidence for methods of their disinfection. Therefore, the objective of this project was to evaluate commercially available disinfectants and disinfection application methods against PEDV and PRRSV on various surfaces within semi-truck cabs. Three different surface types common in vehicle cabs (fabric, plastic, and rubber) were cut into 4 × 4 inch coupons and inoculated with either PEDV or PRRSV. Once inoculated, surfaces were placed in one of 3 semi-truck cabs and the disinfectant treatment was applied. Disinfectant treatments were as follows: 1) no-disinfectant, 2) hurricane fumigation with 1:256 dilution of Synergize, 3) hurricane fumigation with 1:64 dilution of Intervention, 4) pump sprayer with 1:256 dilution of Synergize, 5) pump sprayer with 1:64 dilution of Intervention, 6) pump sprayer with 10% bleach, 7) no chemical with 10 hr downtime, and 8) gaseous fumigation over a 10 hr period with water-based chlorine dioxide. Once a disinfectant treatment was applied, the coupons were environmentally swabbed and submitted for qPCR duplex analysis for PEDV and PRRSV. There was a significant disinfectant × surface interaction (P \u3c 0.0001) indicating that the disinfectant treatment efficacy differed based on surface. Within rubber surfaces, 10% bleach had a greater Ct value compared to all other treatments (P \u3c 0.05), with the exception of Intervention with hurricane fumigation application, which was intermediate. In both fabric and plastic surfaces, there was no evidence (P \u3e 0.05) of a difference in Ct value between any of the treatments. Additionally, for the no-disinfectant treatment, the Ct value was greater on fabric surfaces compared to plastic and rubber (P \u3c 0.05); fabric was greater than plastic in the Intervention with pump sprayer application treatment (P \u3c 0.05), fabric and rubber greater than plastic in the 10% bleach treatment (P \u3c 0.05); and fabric greater than plastic and rubber in the 10 hr downtime and gaseous fumigation treatments (P \u3c 0.05). There was a significant main effect of disinfectant treatment (P = 0.016), where 10% bleach had a greater Ct value compared to both the control treatment, 10 hr downtime treatment, and Intervention applied using the pump sprayer (P \u3c 0.05). There was a main effect of surface (P \u3c 0.0001) where rubber had a greater Ct value compared to plastic (P \u3c 0.05), and fabric had a greater Ct value compared to both rubber and plastic (P \u3c 0.05). Finally, the Ct value for PRRSV was greater than PEDV (P \u3c 0.0001) when averaged across all surfaces and disinfectant treatments. In summary, these data highlight that it is important to consider the surface of interest when implementing disinfectant protocols. In general, most disinfectant applications were only able to reduce the quantity of detectable virus, but not completely eliminate it from surface. However, additional research is necessary to understand the viability of residual virus on disinfected surfaces

Evaluating a Dry vs. Wet Disinfection in Boot Baths on Detection of Porcine Epidemic Diarrhea Virus and Porcine Reproductive and Respiratory Syndrome Virus RNA

Maintaining biosecurity between swine barns is challenging, and boot baths are an easily implementable option some utilize to limit pathogen spread. However, there are concerns regarding their efficacy, especially when comparing wet or dry disinfectants. The objective of this study was to evaluate the efficacy of boot baths in reducing the quantity of detectable porcine epidemic diarrhea virus (PEDV) and porcine reproductive and respiratory syndrome virus (PRRSV) genetic material using wet or dry disinfectants. Treatments included 1) control; 2) dry chlorine powder (Traffic C.O.P., PSP, LLC, Rainsville, AL); and 3) wet quaternary ammonium/glutaraldehyde liquid (1:256 Synergize, Neogen, Lexington, KY). Prior to disinfection, rubber boots were inoculated with 1 mL of co-inoculants of PRRSV (1×105TCID50/mL) and PEDV (1×105 TCID50/mL) and dried for 15 min. After the drying period, a researcher placed the boot on the right foot and stepped directly on a stainless steel coupon (control). Alternatively, the researcher stepped first into a boot bath containing either the wet or dry sanitizer, stood for 3 s, and then stepped onto a steel coupon. After one min, an environmental swab was then collected and processed from each boot and steel coupon. The procedure was replicated 12 times per disinfectant treatment. Samples were analyzed using a duplex qPCR at the Kansas State Veterinary Diagnostic Laboratory. Cycle threshold values, which indicate the presence or absence of the inoculants and their relative concentrations when present, were analyzed using SAS GLIMMIX (v. 9.4, SAS Institute, Inc., Cary, NC). There was no evidence of a disinfectant × surface × virus interaction (P \u3e 0.10). An interaction between disinfectant × surface impacted (P \u3c 0.05) the quantity of detectable viral RNA. As expected, the quantity of the viruses on the coupon were greatest in the control, indicating that a contaminated boot has the ability to transfer viruses from a contaminated surface to a clean surface. Comparatively, the dry disinfectant treatment resulted in no detectable viral RNA on either the boot or subsequent coupon. The wet disinfectant treatment had statistically similar (P \u3e 0.05) viral contamination to the control on the boot, but less viral contamination compared to the control on the metal coupon. In this experiment, a boot bath with dry powder was the most efficacious in reducing the detectable viral RNA on both boots and subsequent surfaces

Evaluating a Dry vs. Wet Disinfection in Boot Baths on Detection of Porcine Epidemic Diarrhea Virus and Porcine Reproductive and Respiratory Syndrome Virus RNA

Maintaining biosecurity between swine barns is challenging, and boot baths are an easily implementable option some utilize to limit pathogen spread. However, there are concerns regarding their efficacy, especially when comparing wet or dry disinfectants. The objective of this study was to evaluate the efficacy of boot baths in reducing the quantity of detectable porcine epidemic diarrhea virus (PEDV) and porcine reproductive and respiratory syndrome virus (PRRSV) genetic material using wet or dry disinfectants. Treatments included 1) control; 2) dry chlorine powder (Traffic C.O.P., PSP, LLC, Rainsville, AL); and 3) wet quaternary ammonium/glutaraldehyde liquid (1:256 Synergize, Neogen, Lexington, KY). Prior to disinfection, rubber boots were inoculated with 1 mL of co-inoculants of PRRSV (1×105TCID50/mL) and PEDV (1×105 TCID50/mL) and dried for 15 min. After the drying period, a researcher placed the boot on the right foot and stepped directly on a stainless steel coupon (control). Alternatively, the researcher stepped first into a boot bath containing either the wet or dry sanitizer, stood for 3 s, and then stepped onto a steel coupon. After one min, an environmental swab was then collected and processed from each boot and steel coupon. The procedure was replicated 12 times per disinfectant treatment. Samples were analyzed using a duplex qPCR at the Kansas State Veterinary Diagnostic Laboratory. Cycle threshold values, which indicate the presence or absence of the inoculants and their relative concentrations when present, were analyzed using SAS GLIMMIX (v. 9.4, SAS Institute, Inc., Cary, NC). There was no evidence of a disinfectant × surface × virus interaction (P \u3e 0.10). An interaction between disinfectant × surface impacted (P \u3c 0.05) the quantity of detectable viral RNA. As expected, the quantity of the viruses on the coupon were greatest in the control, indicating that a contaminated boot has the ability to transfer viruses from a contaminated surface to a clean surface. Comparatively, the dry disinfectant treatment resulted in no detectable viral RNA on either the boot or subsequent coupon. The wet disinfectant treatment had statistically similar (P \u3e 0.05) viral contamination to the control on the boot, but less viral contamination compared to the control on the metal coupon. In this experiment, a boot bath with dry powder was the most efficacious in reducing the detectable viral RNA on both boots and subsequent surfaces

Search for squarks and gluinos in events with isolated leptons, jets and missing transverse momentum at s√=8 TeV with the ATLAS detector

The results of a search for supersymmetry in final states containing at least one isolated lepton (electron or muon), jets and large missing transverse momentum with the ATLAS detector at the Large Hadron Collider are reported. The search is based on proton-proton collision data at a centre-of-mass energy s√=8 TeV collected in 2012, corresponding to an integrated luminosity of 20 fb−1. No significant excess above the Standard Model expectation is observed. Limits are set on supersymmetric particle masses for various supersymmetric models. Depending on the model, the search excludes gluino masses up to 1.32 TeV and squark masses up to 840 GeV. Limits are also set on the parameters of a minimal universal extra dimension model, excluding a compactification radius of 1/R c = 950 GeV for a cut-off scale times radius (ΛR c) of approximately 30

Evidence for the Higgs-boson Yukawa coupling to tau leptons with the ATLAS detector

Results of a search for H → τ τ decays are presented, based on the full set of proton-proton collision data recorded by the ATLAS experiment at the LHC during 2011 and 2012. The data correspond to integrated luminosities of 4.5 fb−1 and 20.3 fb−1 at centre-of-mass energies of √s = 7 TeV and √s = 8 TeV respectively. All combinations of leptonic (τ → `νν¯ with ` = e, µ) and hadronic (τ → hadrons ν) tau decays are considered. An excess of events over the expected background from other Standard Model processes is found with an observed (expected) significance of 4.5 (3.4) standard deviations. This excess provides evidence for the direct coupling of the recently discovered Higgs boson to fermions. The measured signal strength, normalised to the Standard Model expectation, of µ = 1.43 +0.43 −0.37 is consistent with the predicted Yukawa coupling strength in the Standard Model

Measurement of the t t-bar production cross section in the dilepton channel in pp collisions at sqrt(s) = 7 TeV

The t t-bar production cross section (sigma[t t-bar]) is measured in proton-proton collisions at sqrt(s) = 7 TeV in data collected by the CMS experiment, corresponding to an integrated luminosity of 2.3 inverse femtobarns. The measurement is performed in events with two leptons (electrons or muons) in the final state, at least two jets identified as jets originating from b quarks, and the presence of an imbalance in transverse momentum. The measured value of sigma[t t-bar] for a top-quark mass of 172.5 GeV is 161.9 +/- 2.5 (stat.) +5.1/-5.0 (syst.) +/- 3.6(lumi.) pb, consistent with the prediction of the standard model.Comment: Replaced with published version. Included journal reference and DO