RNAseq analysis of different Hevea genus inoculated by Microcyclus ulei : S01P09

The rubber tree, Hevea is native from the Brazilian Amazon region and it is responsible for almost all natural rubber produced in the world, a strategic raw material for a variety of products. One of major challenges for rubber tree cultivation is the adaptation to biotic stress caused by several leaf pathogens. Microcyclus ulei, causal agent of the South American leaf blight (SALB) is responsible of massive losses of leaves by infection, leading to a low latex production and sometimes leading to plant death. The main proposed strategies to avoid the M. ulei damaging on plantations are to cultivate productive and SALB resistant genotypes. In Hevea, quantitative and qualitative resistance to M. ulei can be found in cultivars and but also between genus. By transcriptomic comparison of RNAseq of three genus of Hevea (H. brasiliensis cv. MDF180 and cv. PB314, H. pauciflora cv. PA31 and H. benthamiana cv. F4542), our purpose was to identify genes differentially expressed and characteristic of each genus. Hevea leaf lesions and non-inoculated controls were collected 24, 48, 96 and168 hpi. For each cultivar inoculated or non-inoculated, total RNA of time samples were pooled and enriched in mRNA. The eight cDNA libraries were sequenced with 454 XL+ sequencing kit and produced a total 4,225,291 reads de novo assembled on 28,349 contigs. A tBLASTx against a NCBI plant RefSeq was performed and 15,442 sequences were successfully annotated. A differential expression analysis was conducted to each library with the NOIseq 2.0 software (Bioconductor) and for a cutoff of q score of 0.9, we identified in F4542 cultivar (quantitative resistance) 89 up-regulated and 32 down-regulated genes, in MDF180 cultivar (quantitative resistance), 117 up- and 71 down-regulated genes, in PA31cultivar (quantitative resistance), 72 up- and 12 down-regulated genes and in PB314 cultivar (susceptible) 455 up-regulated and 169 down-regulated genes. This preliminary analysis provides a first look at the host and parasite transcriptome of Hevea spp. Further analyses are underway to distinguish the host genes from those of the parasite and identify with more accuracy the genes differentally expressed in each Hevea genus during the infection. Work supported by CAPES/CNPq. (Texte intégral

Measurement of the Bottom contribution to non-photonic electron production in p+pp+p collisions at s\sqrt{s} =200 GeV

The contribution of $B$ meson decays to non-photonic electrons, which are mainly produced by the semi-leptonic decays of heavy flavor mesons, in $p+p$ collisions at $\sqrt{s} =$ 200 GeV has been measured using azimuthal correlations between non-photonic electrons and hadrons. The extracted $B$ decay contribution is approximately 50% at a transverse momentum of $p_{T} \geq 5$ GeV/$c$. These measurements constrain the nuclear modification factor for electrons from $B$ and $D$ meson decays. The result indicates that $B$ meson production in heavy ion collisions is also suppressed at high $p_{T}$.Comment: 6 pages, 4 figures, accepted by PR

Longitudinal double-spin asymmetry and cross section for inclusive neutral pion production at midrapidity in polarized proton collisions at sqrt(s) = 200 GeV

We report a measurement of the longitudinal double-spin asymmetry A_LL and the differential cross section for inclusive Pi0 production at midrapidity in polarized proton collisions at sqrt(s) = 200 GeV. The cross section was measured over a transverse momentum range of 1 < p_T < 17 GeV/c and found to be in good agreement with a next-to-leading order perturbative QCD calculation. The longitudinal double-spin asymmetry was measured in the range of 3.7 < p_T < 11 GeV/c and excludes a maximal positive gluon polarization in the proton. The mean transverse momentum fraction of Pi0's in their parent jets was found to be around 0.7 for electromagnetically triggered events.Comment: 6 pages, 3 figures, submitted to Phys. Rev. D (RC

Measurement of the cross section for isolated-photon plus jet production in pp collisions at √s=13 TeV using the ATLAS detector

The dynamics of isolated-photon production in association with a jet in proton–proton collisions at a centre-of-mass energy of 13 TeV are studied with the ATLAS detector at the LHC using a dataset with an integrated luminosity of 3.2 fb−1. Photons are required to have transverse energies above 125 GeV. Jets are identified using the anti- algorithm with radius parameter and required to have transverse momenta above 100 GeV. Measurements of isolated-photon plus jet cross sections are presented as functions of the leading-photon transverse energy, the leading-jet transverse momentum, the azimuthal angular separation between the photon and the jet, the photon–jet invariant mass and the scattering angle in the photon–jet centre-of-mass system. Tree-level plus parton-shower predictions from Sherpa and Pythia as well as next-to-leading-order QCD predictions from Jetphox and Sherpa are compared to the measurements

A search for resonances decaying into a Higgs boson and a new particle X in the XH → qqbb final state with the ATLAS detector

A search for heavy resonances decaying into a Higgs boson (H) and a new particle (X) is reported, utilizing 36.1 fb−1 of proton–proton collision data at collected during 2015 and 2016 with the ATLAS detector at the CERN Large Hadron Collider. The particle X is assumed to decay to a pair of light quarks, and the fully hadronic final state is analysed. The search considers the regime of high XH resonance masses, where the X and H bosons are both highly Lorentz-boosted and are each reconstructed using a single jet with large radius parameter. A two-dimensional phase space of XH mass versus X mass is scanned for evidence of a signal, over a range of XH resonance mass values between 1 TeV and 4 TeV, and for X particles with masses from 50 GeV to 1000 GeV. All search results are consistent with the expectations for the background due to Standard Model processes, and 95% CL upper limits are set, as a function of XH and X masses, on the production cross-section of the resonance

Feed training of juvenile giant trahira under different light intensities

The aim of this study was to evaluate the influence of light intensity on the feed training of juvenile giant trahiras (Hoplias lacerdae). A completely randomized design with four light treatments and three replicates was used, and the following light intensities were tested: 204.9; 177.58; 54.64 and 0.00 lux. Juvenile giant trahiras (length = 3.0 ± 0.4 cm and weight = 0.5 ± 0.05 g) were stocked at 6 fish L-1 in 12 rectangular tanks (24 × 20 × 14 cm) containing six liters of water, and subjected to feed training to accept dry diets. At the end of the experiment (21 days), weight gain, length gain, survival, cannibalism, and mortality rates were evaluated. Data were subjected to regression analysis at 5% probability. Different light intensities did not affect growth, survival, mortality, or cannibalism rates in juvenile giant trahiras during the feed training. Therefore, this species can be trained to accept dry diets in the presence or absence of light

Microbial and fermentation profiles, losses and chemical composition of silages of buffel grass harvested at different cutting heights

The present study evaluated the microbial population, fermentation profile, losses and dry matter recovery, and chemical composition of silages of buffel grass at different cutting heights. To evaluate the microbial fermentation dynamics, the treatments resulted from a 4 × 5 factorial combination consisting of 4 cutting heights and 5 fermentation periods, in a completely randomized design with three replications. The fermentation was evaluated at the end of 1, 3, 7, 15 and 30 days. The other characteristics of silages with 30 days were evaluated following a completely randomized design with four treatments, consisting of 4 cutting heights (30, 40, 50 and 60 cm), and five replications. Fermentation period and cutting height effects and interaction between both factors were observed on the populations of lactic acid bacteria (LAB), enterobacteria and molds and yeasts. The peak of development of LAB populations was observed on the seventh day of fermentation for the heights of 40 and 50 cm, with 8.25 and 8.30 log cfu/g, respectively. The pH values of silages ranged with different cutting heights, in which at the height of 50 cm the decrease was most pronounced. However, the pH values were similar between the cutting heights at the end of 30 days of fermentation. Quadratic relationship was observed between lactic acid concentrations and cutting heights. The crude protein content behaved linearly, initially showing 128.5 g/kg DM at 30 cm, decreasing as the cutting heights increased. The neutral detergent fiber and ether extract contents increased linearly with the cutting heights. Based on microbial populations, fermentation, losses and chemical composition, it is recommended to harvest buffel grass for silage from 50 cm on

Feed training of giant trahira fingerlings fed diets containing different levels of vitamin C

The objective of this work was to evaluate suplemenation of vitamin C during feed training of giant trahira fingerlings. An experiment was established in a complete random design with seven treatments (0.0, 17.5, 52.5, 87.5, 122.5, 175.0 and 350.0 mg vitamin C/kg diet) and four repetitions. Giant trahira fingerlings (2.8 ± 0.2 cm) were distributed in 6-L aquaria at the density of 6 fish/L. A basal diet was formulated with 44.0% of crude protein and vitamin C and bovine heart were added in it daily. After 20 days, biometry of fish for evaluation of weight and length gains, rates of survival and cannibalism and uniformity of final length and for macroscopic observations of clinical signs of deficiency or excess of vitamin was carried out. After biometry evaluation, ten fish from each tested diet were collected for analysis of the fatty acid profile which was compared to profile of fish at the same life stage fed forage fish collected from breeding fishponds. Data on growth performance and fatty acid profiles were submitted to analyses of polynomial regression and uniformity of the final length was evaluated by the Bartlett test. There was a significant difference only for uniformity in final length and fatty acid profile of the carcass. Supplementation with 52.5 mg vitamin C/kg diet provided a greater uniformity of the final length of the fish. Fish from breeding fishponds presented lower concentration of PUFA (polyunsaturated fatty acids) and higher concentration of saturated fatty acids compared to fish trained to accept dry diets.Com este trabalho objetivou-se avaliar a suplementação de vitamina C durante a fase de condicionamento alimentar de alevinos de trairão. Um experimento foi estabelecido em delineamento inteiramente casualizado com sete tratamentos (0,0; 17,5; 52,5; 87,5; 122,5; 175,0 e 350,0 mg de vitamina C/kg de ração) e quatro repetições. Alevinos de trairão (2,8 ± 0,2 cm) foram distribuídos em aquários de 6 L, na densidade de 6 peixes/L. Uma ração basal foi confeccionada com 44,0% proteína bruta, e diariamente foi adicionada vitamina C e coração bovino. Após 20 dias, realizou-se biometria dos peixes para avaliação do ganho de peso e do comprimento, das taxas de sobrevivência e canibalismo e da uniformidade do comprimento final e para observações macroscópicas de sinais clínicos de deficiência ou excesso da vitamina. Após a biometria, dez peixes de cada dieta testada foram coletados para análise do perfil de ácidos graxos e este perfil foi comparado ao perfil de peixes, na mesma fase de vida, alimentados com peixes forrageiros coletados de viveiros de criação. Os dados de desempenho produtivo e perfil de ácidos graxos foram submetidos a análise de regressão polinomial e a uniformidade do comprimento final avaliada pelo teste de Bartlett. Houve diferença significativa apenas para a uniformidade em comprimento final e perfil de ácidos graxos da carcaça. A suplementação com 52,5 mg de vitamina C/kg de ração proporcionou maior uniformidade do comprimento final dos peixes. Os peixes dos viveiros de criação apresentaram menor concentração de PUFA (polyunsaturated fatty acids) e maior de ácidos graxos saturados em comparação aos peixes condicionados a aceitar dietas secas