In vitro selective growth-inhibitory effect of 8-hydroxyquinoline on Clostridium perfringens versus Bifidobacteria in a medium containing chicken ileal digesta

Clostridium perfringens-induced necrotic enteritis is generally controlled by antibiotics. However, because of increasing antibiotic resistance, other antibacterial agents are required, preferably ones that do not affect the beneficial intestinal microbiota of the host. This study evaluated the in vitro selective growth-inhibitory effect of 8-hydroxyquinoline (8HQ) on C. perfringens vs. bifidobacteria in a medium containing chicken ileal digesta. Prior to the experiments, the minimum inhibitory concentrations of 8HQ and penicillin G were determined by broth microdilution assay. The minimum inhibitory concentration values of 8HQ for C. perfringens were 16-32 times lower than the values for bifidobacteria. Treatment of autoclaved and non-autoclaved chicken ileal digesta with 8HQ showed a selective anticlostridial effect. After incubation of C. perfringens with autoclaved ileal digesta for 3 h, all 8HQ concentrations tested (32-2048 mu g/mL) significantly reduced C. perfringens bacterial count. In contrast, the same treatment had no or only a slight effect on bifidobacteria counts. Unlike 8HQ, penicillin G did not exhibit any selectivity. Similar results were obtained after incubation for 24 h. In non-autoclaved ileal digesta, all 8HQ concentrations tested significantly reduced C. perfringens bacterial counts after incubation for 30 min and 3 h, while no effect was observed on bifidobacteria. These results suggest that 8HQ may serve as a prospective veterinary compound for use against necrotic enteritis in poultry

In vitro growth-inhibitory activity of Calophyllum inophyllum ethanol leaf extract against diarrhoea-causing bacteria

Purpose: To investigate the in vitro growth-inhibitory effect of Calophyllum inophyllum, a medicinal plant traditionally used to cure gastrointestinal disorders caused by diarrhoea-causing bacteria.Methods: The minimum inhibitory concentration (MIC) of C. inophyllum ethanol leaf extract was determined against six diarrhoea-causing bacteria, namely, Clostridium difficile infant, Clostridium perfringens, Enterococcus faecalis, Escherichia coli, Listeria monocytogenes, and Salmonella enterica. The effect of the plant extract on bacterial growth kinetics was further evaluated by slightly modified broth microdilution method.Results: The plant extract showed significant inhibitory activity against C. perfringens and L. monocytogenes (MIC = 128 μg/mL) followed by C. difficile (MIC = 512 μg/mL). Monitored growth curves also showed that the plant extract at ½ MIC inhibits bacterial growth by distinct extension of the lag phase or suppression of the whole growth rate in C. difficile and L. monocytogenes, respectively.Conclusion: These results demonstrate the significant anti-clostridial and anti-listerial activities of C. inophyllum ethanol leaf extract. Thus, the extract seems to be a promising material for the development of new antibacterial agents.Keywords: Calophyllum inophyllum, Alexandrian laurel, Intestinal infections, Antibacterial activity, Anticlostridial, Anti-listeria

Nutritive value of palm kernel meal in diets for growing rabbits

The aim of this work was to determine the nutritive value of palm kernel meal (PKM) in diets for growing rabbits. In Experiment 1, 20 New Zealand × Californian growing rabbits 50 d-old were used to determine energy, crude protein, fibre and fat digestibility of PKM. The nutritive value was estimated by the difference method using a basal diet and another diet made by substituting 200 g/kg of basal diet with PKM. Energy, crude protein, ether extract and neutral detergent fibre of PKM digestibilities were, respectively, 0.549 (±0.056, SE), 0.541 (±0.069), 0.850 (±0.048) and 0.430 (±0.101), and the digestible energy concentration was 10.9 MJ/kg (±1.03) DM. In Experiment 2, 412 rabbits were allocated at random to the two experimental diets to measure growing performance. Inclusion of 200 g PKM/kg in the diet did not affect feed or digestible energy intake but decreased slightly (by around 5%) average daily gain (P = 0.003) and feed efficiency (P < 0.001). Neither mortality nor Clostridium perfringens counts in soft faeces were affected by type of diet. Palm kernel meal can be considered a palatable source of fibre, protein and fat for rabbits and can substitute significant amounts of other fibrous ingredients in the diet without adverse effects on growth performance

Testing the efficacy of medium chain fatty acids against rabbit colibacillosis

Enteropathogenic Escherichia coli (EPEC) represents a major cause of lethal diarrhea in young mammals. Although the pathogenicity mechanisms of EPEC are now well understood, the intrinsic and environmental factors that control the expression of EPEC virulence remain largely unknown. In the rabbit, suckling reduces pups’ sensitivity to EPEC infection. Hence, we have hypothesized that uncharacterized factors present in doemilkmay mediate this protection. Medium chain fatty acids (MCFA), known to possess antimicrobial properties, are highly abundant in doe milk.We demonstrate that caprylic acid exhibits a clear bacteriostatic effect in vitro against the rabbit EPEC strain E22 (O103:H2:K-), in a dose-dependent manner. In vivo, the dietary inclusion of triglycerides of MCFA did not however reduce the sensitivity of young rabbits challenged with this EPEC strain. The mortality and fecal excretion of EPEC were not reduced, and the bacterial adhesion to ileum was not inhibited. Amount of MCFA reaching the ileal level might have been too low and/or their association to other milk antimicrobials may have been required to observe a positive effect on disease evolution in a context of a highly virulent challenge

Effects of dietary supplementation of selenium and iodine on growth performance, carcass characteristics and histology of thyroid gland in goats

This study assessed the effects of dietary selenium (Se), iodine (I) and a combination of both on growth performance, thyroid gland activity, carcass characteristics and the concentration of iodine and selenium in Longissimus lumborum (LL) muscle in goats. Twenty-four bucks were randomly assigned to four dietary treatments: control (CON), basal diet without supplementation, basal diet + 0.6 mg Se/kg dry matter (DM) (SS), 0.6 mg I/kg DM (IP), or combination of 0.6 mg/kg DM Se and 0.6 mg/kg DM I (SSIP) and fed for 100 days. Animals fed diet SSIP exhibited higher (P < 0.05) body weight and better feed conversion ratio (FCR) than those fed other diets. Dressing percentage of goats fed the supplemented diets was higher (P < 0.05) than that of the control. Carcasses from the IP group had higher (P < 0.05) total fat proportion than the SSIP group. The levels of both elements were significantly elevated (P < 0.05) in LL muscle in supplemented goats. Thyroid follicular epithelial cells of IP and SSIP animals were significantly higher than those of CON and SS groups. The study demonstrated that the combined Se and I dietary supplementation improves growth performance, carcass dressing percentage and increases the retention of Se and I in goat meat

Metodología de valoración de paisaje empleando ArcGIS: Aplicación a las Vías Pecuarias

The concept of landscape is assuming an increasing importance in legislation, and the Natural Heritage and Biodiversity Law requires a analysis of the landscape for the Natural Resources Ordination Plans. This calls for a range of tools to assist in studying the landscape prior to the assessment of management plans for natural resources. The advances in the techniques employed by Geographic Information Systems for assessing the landscape can be enhanced by the incorporation of numerous variables into the analysis. This work presents a landscape assessment methodology using GIS tools and including a number of variables which evaluate the landscape in terms of the viewshed and the distance from the observation point, applied to the specific case of the traditional cattle migration routes.<br><br>El concepto de paisaje cada vez adquiere mayor importancia en la legislación porque también es importante el estudio del paisaje y todas las herramientas que pueden ayudar a su valoración en la gestión de los recursos naturales, ya que dentro de la Ley de Patrimonio Natural y Biodiversidad, es necesario el estudio del paisaje en los Planes de Ordenación de los Recursos Naturales. El avance de las técnicas que utilizan los Sistemas de Información Geográfica para la evaluación del paisaje permite agilizar su valoración con la introducción de numerosas variables que se incorporan en el análisis. Este trabajo presenta una metodología de valoración del paisaje utilizando herramientas SIG, introduciendo variables y valorándolas en función de la cuenca visual y de la distancia al punto de observación, aplicándolo al caso particular de las Vías Pecuarias

Inhibitory effects of the macrolide antimicrobial tylosin on anaerobic treatment

A laboratory-scale anaerobic sequencing batch reactor (ASBR) was operated using a glucose-based synthetic wastewater to study the effects of tylosin, a macrolide antimicrobial commonly used in swine production, on treatment performance. The experimental period was divided into three consecutive phases with different influent tylosin concentrations (0, 1.67, and 167 mg/L). The addition of 1.67 mg/L tylosin to the reactor had negligible effects on the overall treatment performance, that is, total methane production and effluent chemical oxygen demand did not change significantly ( P  < 0.05), yet analyses of individual ASBR cycles revealed a decrease in the rates of both methane production and propionate uptake after tylosin was added. The addition of 167 mg/L tylosin to the reactor resulted in a gradual decrease in methane production and the accumulation of propionate and acetate. Subsequent inhibition of methanogenesis was attributed to a decrease in the pH of the reactor. After the addition of 167 mg/L tylosin to the reactor, an initial decrease in the rate of glucose uptake during the ASBR cycle followed by a gradual recovery was observed. In batch tests, the specific biogas production with the substrate butyrate was completely inhibited in the presence of tylosin. This study indicated that tylosin inhibited propionate- and butyrate-oxidizing syntrophic bacteria and fermenting bacteria resulting in unfavorable effects on methanogenesis. Biotechnol. Biotechnol. Bioeng. 2008;101: 73–82. © 2008 Wiley Periodicals, Inc.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/60471/1/21864_ftp.pd

Volatile Constituents, Inorganic Elements and Primary Screening of Bioactivity of Black Coral Cigarette Holders

Black corals (BC) have been used for a long time in Chinese medicine, and may have some pharmaceutical functions when used as material for cigarette holders in southeast China. This study is aimed to investigate the bioactivities of volatile constituents in BC and to explore the folklore behind the use of BC cigarette holders (BCCHs). We extracted the volatile constituents of BC by supercritical fluid extraction (SFE) with carbon dioxide (CO2-SFE), then identified and analyzed the constituents by gas chromatography-mass spectrometry (GC-MS). In total, 15 components were reliably identified in BC and found to be biologically active. These included triethyl phosphate, butylated hydroxytoluene, cedrol, n-hexadecanoic acid, squalene, and cholesterol. Meanwhile 13 inorganic elements (P, Ca, Mg, S, B, Si, Fe, Cu, Zn, Ba, etc.) were determined by inductively coupled plasma spectrometer (ICPS). In the bioactivity tests, the BC extract (BCE) showed a scavenging activity of 2,2-diphenyl-1-picrylhydrazyl free radicals and hydroxyl radicals by phenanthroline-Fe (II) oxidation and moderate inhibition of Gram-positive microorganisms. The antioxidant and antimicrobial activities of BC, which are related to the active chemical composition, may explain the perceived benefit for cigarette smokers who use BCCHs

The use of nano/micro-layers, self-healing and slow release coatings to prevent corrosion and biofouling

The mitigation of corrosion and biofouling is a challenge. Through application of chemicals and special techniques can slow these undesired processes, an effective resolution requires a multidisciplinary approach involving scientists, engineers, and metallurgists. In order to understand the importance of the use of nano- and microlayers as well as self-healing coatings, the basic concepts of corrosion, corrosion mechanisms, corrosion inhibition and the microbiologically influenced corrosion will be summarised. The preparation, characterization and application of Langmuir-Blodgett and self assembled nanolayers in corrosive and microbial environment will be discussed. Preparation and characterization of microcapsules/ microspheres and their application in coatings will be demonstrated by a number of examples