In-vitro Anti-Cancer Screening of Solanumindicum Rhus succedanea, Rheum emodiand Gardenia gummifera Medicinal Plants in Cancer Cells

ABSTRACT The anticancer activities of Solanumindicum, Rhus succedaneum, Rheum emodi, and Gardenia gummifera medicinal plants from the methanolic extracts were determined by using in-vitroMTT cytotoxicity assay in various cancer cell lines human non-small cell lung carcinoma(H1975), prostatecarcinoma (PC-3and DU145), colorectal carcinoma (HCT116) and malignant melanoma (A375). The invitrocytotoxicity assay revealed that S. indicum, R. succedanea, R. emodi and G. gummifera extracts had strongest cytotoxicity with IC50 of 8.48 g/ml,24.5g/ml, 28.24g/ml and 28.61g/ml respectively in DU145 cells and also with IC50 of 11.18g/ml, 11.04 g/ml, 11.05 g/ml and 23.03g/ml respectively in PC-3 cells. Whereas these extracts exhibited cytotoxicity with IC50 of 9.03g/ml, 7.71g/ml, 15.95 g/ml and 11.71g/ml respectively in H1975 cells. Similarly all these extracts showed cytotoxicity with IC50 of 17.58g/ml,8.87g/ml,19.31g/ml and 11.67 g/ml respectively in HCT116 cells. Finally methanolic extracts of these extracts exhibited cytotoxicity with IC 50 27.94 g/ml, 13.13g/ml, 11.05 g/ml and 7.816g/ml respectively in A375 cells. Our results revealed that methanolic extract of S

ZnO nanopellets have selective anticancer activity

This research work presents the synthesis of ZnO nanopellets (ZNPs) by low temperature hydrothermal approach and evaluation of their antibacterial activity, cytotoxicity in vitro and in vivo. Structural and morphological studies conducted on the sample reveal hexagonal ZNPs in the size range of 250–500nm. Surface area measurements showed high porosity of the sample compared to conventional ZnO nanoparticles. Antimicrobial studies revealed their bactericidal nature against both Gram-negative and Gram-positive bacteria. Furthermore, to better understand the parameters that affect the interactions between our ZNPs and mammalian cells, and thus their biocompatibility, we have examined the impact of cell culture conditions as well as of material properties on cytotoxicity by DPPH, blood hemolysis and MTT assay. The results showed good antioxidant capacity and biocompatibility of ZNPs at higher concentrations. MTT assay revealed the anticancer activity of ZNPs against prostate and breast cancer cell lines. Acute toxicity tests on Swiss albino mice showed no evident toxicity over a 14 days period