Population Biology And Distribution Of The Tanaid Kalliapseudes Schubarti Mañé-garzon, 1949, In An Intertidal Flat In Southeastern Brazil.

The population biology and the spatial and temporal distribution of Kalliapseudes schubarti Mañé-Garzon, 1949, a common tanaidacean in mud flats and estuaries in southern and southeastern Brazil, was studied in the Araçá region, São Sebastião (SP), Brazil. This species showed a clustered dispersion in the area and the individuals were concentrated in the superficial sediment layer (5 cm). Higher densities of K. schubarti were recorded in areas characterized by moderately sorted fine sediment. Multiple regression analysis revealed a positive influence of the organic matter contents and a negative effect of the silt-clay contents on the abundance of K. schubarti. This species showed a marked temporal variation with very low abundance in winter and fall (March to August). Sexual dimorphism was evidenced with males being larger than females. Ovigerous females were also larger than pre-ovigerous ones. Sex ratio was skewed towards females. Seven cohorts were identified during the sampling period, the estimated longevity was 12 months, and no seasonal oscillation in growth was evidenced. The continuous reproduction, as evidenced by the presence of larval phases (manca II and neutron) and reproductive females throughout the year, and high fecundity among the tanaids associated with fast growth and limited longevity support the case for the opportunistic life strategy suggested for this species in the literature.63346947

Effective Action for QED with Fermion Self-Interaction in D=2 and D=3 Dimensions

In this work we discuss the effect of the quartic fermion self-interaction of Thirring type in QED in D=2 and D=3 dimensions. This is done through the computation of the effective action up to quadratic terms in the photon field. We analyze the corresponding nonlocal photon propagators nonperturbatively in % \frac{k}{m}, where k is the photon momentum and m the fermion mass. The poles of the propagators were determined numerically by using the Mathematica software. In D=2 there is always a massless pole whereas for strong enough Thirring coupling a massive pole may appear . For D=3 there are three regions in parameters space. We may have one or two massive poles or even no pole at all. The inter-quark static potential is computed analytically in D=2. We notice that the Thirring interaction contributes with a screening term to the confining linear potential of massive QED_{2}. In D=3 the static potential must be calculated numerically. The screening nature of the massive QED$_{3}$ prevails at any distance, indicating that this is a universal feature of % D=3 electromagnetic interaction. Our results become exact for an infinite number of fermion flavors.Comment: Latex, 13 pages, 3 figure

Insights into the mechanism of MCT8 oligomerization

Mutations in the thyroid hormone transporter monocarboxylate transporter 8 (MCT8) result in MCT8 deficiency, characterized by severe intellectual and motor disability. The MCT8 protein is predicted to have 12 transmembrane domains (TMDs) and is expressed as monomers, homodimers, and homo-oligomers. This study aimed to delineate the mechanism o

Insights into the mechanism of MCT8 oligomerization

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Efectos del proceso de secado sobre la composición en ácidos grasos, perfil fenólico, tocoferoles y actividad antioxidante de almendras barú (Dipteryx alata Vog.)

This study carried out a chromatographic and spectrophotometric characterization of the bioactive compounds, antioxidants, phenolics, tocopherols, sterols and fatty acids of baru almonds “in natura” and submitted to drying processes. It was determined that baru “in natura” almonds presented high levels of phenolic compounds, vitamin C, antioxidants, phenolics, sterols, total monounsaturated fatty acids and low thrombogenic, and atherogenic indexes. During the process of drying it at 65 °C for 30 minutes, a decrease was noted in the levels of caffeic acid, chlorogenic acid, anthocyanins, p-coumaric acid, ferulic acid, o-coumaric acid, quercetin, and polyunsaturated fatty acids. The same condition resulted in an increase in the levels of gallic acid, rutin, catechin, trans-cinnamic acid, vanillin, m-coumaric acid, tocopherols, monounsaturated fatty acids and antioxidant activity (ORAC and DPPH). When submitted to a temperature of 105 ºC for 30 minutes the same behavior was seen with a reduction in the vitamin C and ORAC contents and increased presence of flavonoids.Este estudio realizó una caracterización cromatográfica y espectrofotométrica de la presencia de compuestos bioactivos, antioxidantes, fenólicos, tocoferoles, esteroles y ácidos grasos en almendras del tipo baru “in natura” y sometidos a procesos de secado. Se detectó, en la almendra de baru “in natura”, altos contenidos de compuestos fenólicos, vitamina C, antioxidantes fenólicos, esteroles, ácidos grasos monoinsaturados totales y bajos índices de trombogénicos y aterogénicos. Durante el proceso de secado a 65 °C durante 30 minutos, se observó una disminución en los niveles de ácido cafeíco, ácido clorogénico, antocianinas, ácido p-cumárico, ácido ferúlico, ácido o-cumárico, quercetina y ácidos grasos poliinsaturados. De la misma manera se observó un aumento en los niveles de ácido gálico, rutina, catequina, ácido trans-cinámico, vanilina, ácido m-cumárico, tocoferoles, ácidos grasos monoinsaturados y actividad antioxidante (ORAC y DPPH). Cuando se sometió a una temperatura de 105 °C durante 30 minutos, presentó el mismo comportamiento, sin embargo, influyó en la reducción del contenido de vitamina C y ORAC y aumentó la presencia de flavonoides

Kinase Inhibitor Profile For Human Nek1, Nek6, And Nek7 And Analysis Of The Structural Basis For Inhibitor Specificity

Human Neks are a conserved protein kinase family related to cell cycle progression and cell division and are considered potential drug targets for the treatment of cancer and other pathologies. We screened the activation loop mutant kinases hNek1 and hNek2, wild-type hNek7, and five hNek6 variants in different activation/phosphorylation statesand compared them against 85 compounds using thermal shift denaturation. We identified three compounds with significant Tm shifts: JNK Inhibitor II for hNek1(Ä262-1258)-(T162A), Isogranulatimide for hNek6(S206A), and GSK-3 Inhibitor XIII for hNek7wt. Each one of these compounds was also validated by reducing the kinases activity by at least 25%. The binding sites for these compounds were identified by in silico docking at the ATP-binding site of the respective hNeks. Potential inhibitors were first screened by thermal shift assays, had their efficiency tested by a kinase assay, and were finally analyzed by molecular docking. Our findings corroborate the idea of ATP-competitive inhibition for hNek1 and hNek6 and suggest a novel non-competitive inhibition for hNek7 in regard to GSK-3 Inhibitor XIII. Our results demonstrate that our approach is useful for finding promising general and specific hNekscandidate inhibitors, which may also function as scaffolds to design more potent and selective inhibitors.20111761191Rubin, G.M., Yandell, M.D., Wortman, J.R., Gabor Miklos, G.L., Nelson, C.R., Hariharan, I.K., Fortini, M.E., Fleischmann, W., Comparative genomics of the eukaryotes (2000) Science, 287, pp. 2204-2215Johnson, L.N., Lowe, E.D., Noble, M.E., Owen, D.J., The Eleventh Datta Lecture. The structural basis for substrate recognition and control by protein kinases (1998) FEBS Lett., 430, pp. 1-11Hanks, S.K., Eukaryotic protein kinases (1991) Curr. Opin. Struct. Biol., 1, pp. 369-383Jeffrey, P.D., Russo, A.A., Polyak, K., Gibbs, E., Hurwitz, J., Massagué, J., Pavletich, N.P., Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complex (1995) Nature, 376, pp. 313-320Yamaguchi, H., Hendrickson, W.A., Structural basis for activation of human lymphocyte kinase Lck upon tyrosine phosphorylation (1996) Nature, 384, pp. 484-489Canagarajah, B.J., Khokhlatchev, A., Cobb, M.H., Goldsmith, E.J., Activation mechanism of the MAP kinase ERK2 by dual phosphorylation (1997) Cell, 90, pp. 859-869Hubbard, S.R., Crystal structure of the activated insulin receptor tyrosine kinase in complex with peptide substrate and ATP analog (1997) EMBO J, 16, pp. 5572-5581Fry, A.M., O'Regan, L., Sabir, S.R., Bayliss, R., Cell cycle regulation by the NEK family of protein kinases (2012) J. Cell Sci., 125, pp. 4423-4433Meirelles, G.V., Perez, A.M., Souza, E.E., Basei, F.L., Papa, P.F., Melo Hanchuk, T.D., Cardoso, V.B., Kobarg, J., "Stop Ne(c)king around": How systems biology can help to characterize the functions of Nek family kinases from cell cycle regulation to DNA damage response (2014) World J. Biol. Chem., 5, pp. 141-160Fry, A.M., Mayor, T., Meraldi, P., Stierhof, Y.D., Tanaka, K., Nigg, E.A., C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2 (1998) J. Cell Biol., 141, pp. 1563-1574Quarmby, L.M., Mahjoub, M.R., Caught nek-ing: Cilia and centrioles (2005) J. Cell Sci., 118, pp. 5161-5169Meirelles, G.V., Silva, J.C., Mendonça, Y.A., Ramos, C.H., Torriani, I.L., Kobarg, J., Human Nek6 is a monomeric mostly globular kinase with an unfolded short N-terminal domain (2011) BMC Struct. Biol., 11, p. 12Belham, C., Roig, J., Caldwell, J.A., Aoyama, Y., Kemp, B.E., Comb, M., Avruch, J., A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases (2003) J. Biol. Chem., 278, pp. 34897-34909Yin, M.J., Shao, L., Voehringer, D., Smeal, T., Jallal, B., The serine/threonine kinase Nek6 is required for cell cycle progression through mitosis (2003) J. Biol. Chem., 278, pp. 52454-52460Yissachar, N., Salem, H., Tennenbaum, T., Motro, B., Nek7 kinase is enriched at the centrosome, and is required for proper spindle assembly and mitotic progression (2006) FEBS Lett., 580, pp. 6489-6495Kim, S., Lee, K., Rhee, K., NEK7 is a centrosomal kinase critical for microtubule nucleation (2007) Biochem. Biophys. Res.Commun., 360, pp. 56-62Roig, J., Mikhailov, A., Belham, C., Avruch, J., Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression (2002) Genes Dev., 16, pp. 1640-1658Upadhya, P., Birkenmeier, E.H., Birkenmeier, C.S., Barker, J.E., Mutations in a NIMA-related kinase gene, Nek1, cause pleiotropic effects including a progressive polycystic kidney disease in mice (2000) Proc. Natl. Acad. Sci. USA, 97, pp. 217-221Liu, S., Lu, W., Obara, T., Kuida, S., Lehoczky, J., Dewar, K., Drummond, I.A., Beier, D.R., A defect in a novel Nek-family kinase causes cystic kidney disease in the mouse and in zebrafish (2002) Development, 129, pp. 5839-5846Chen, J., Li, L., Zhang, Y., Yang, H., Wei, Y., Zhang, L., Liu, X., Yu, L., Interaction of Pin1 with Nek6 and characterization of their expression correlation in Chinese hepatocellular carcinoma patients (2006) Biochem. Biophys. Res. Commun., 341, pp. 1059-1065Chen, Y., Chen, P.L., Chen, C.F., Jiang, X., Riley, D.J., Never-in mitosis related kinase 1 functions in DNA damage response and checkpoint control (2008) Cell Cycle, 7, pp. 3194-3201Lee, M.Y., Kim, H.J., Kim, M.A., Jee, H.J., Kim, A.J., Bae, Y.S., Park, J.I., Yun, J., Nek6 is involved in G2/M phase cell cycle arrest through DNA damage induced phosphorylation (2008) Cell Cycle, 7, pp. 2705-2709Innocenti, P., Cheung, K.M., Solanki, S., Mas-Droux, C., Rowan, F., Yeoh, S., Boxall, K., Hardy, T., Design of potent and selective hybrid inhibitors of the mitotic kinase Nek2: Structure-activity relationship, structural biology, and cellular activity (2012) J. Med. Chem., 55, pp. 3228-3241Solanki, S., Innocenti, P., Mas-Droux, C., Boxall, K., Barillari, C., Van Montfort, R.L., Aherne, G.W., Hoelder, S., Benzimidazole inhibitors induce a DFG-out conformation of never in mitosis gene A-related kinase 2 (Nek2) without binding to the back pocket and reveal a nonlinear structure-activity relationship (2011) J. Med. Chem., 54, pp. 1626-1639Whelligan, D.K., Solanki, S., Taylor, D., Thomson, D.W., Cheung, K.M., Boxall, K., Mas-Droux, C., Grummitt, C.G., Aminopyrazine inhibitors binding to an unusual inactive conformation of the mitotic kinase Nek2: SAR and structural characterization (2010) J. Med. Chem., 53, pp. 7682-7698Srinivasan, P., ChellaPerumal, P., Sudha, A., Discovery of novel inhibitors for Nek6 protein through homology model assisted structure based virtual screening and molecular docking approaches (2014) Sci. World J., 2014. , ID: 967873Rellos, P., Ivins, F.J., Baxter, J.E., Pike, A., Nott, T.J., Parkinson, D.M., Das, S., Shen, Q.Y., Structure and regulation of the human Nek2 centrosomal kinase (2007) J. Biol. Chem., 282, pp. 6833-6842Westwood, I., Cheary, D.M., Baxter, J.E., Richards, M.W., Van Montfort, R.L., Fry, A.M., Bayliss, R., Insights into the conformational variability and regulation of human Nek2 kinase (2009) J. Mol. Biol, 386, pp. 476-485Richards, M.W., O'Regan, L., Mas-Droux, C., Blot, J.M., Cheung, J., Hoelder, S., Fry, A.M., Bayliss, R., An autoinhibitory tyrosine motif in the cell-cycleregulated Nek7 kinase is released through binding of Nek9 (2009) Mol. Cell, 36, pp. 560-570Geromichalos, G.D., Importance of molecular computer modeling in anticancer drug development (2007) J. Buon., 12, pp. S101-S118Vedadi, M., Niesen, F.H., Allali-Hassani, A., Fedorov, O.Y., Finerty, P.J., Jr., Wasney, G.A., Yeung, R., Berglund, H., Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination (2006) Proc. Natl. Acad. Sci. USA, 103, pp. 15835-15840Lengauer, T., Rarey, M., Computational methods for biomolecular docking (1996) Curr. Opin. Struct. Biol., 6, pp. 402-406Fedorov, O., Marsden, B., Pogacic, V., Rellos, P., Müller, S., Bullock, A.N., Schwaller, J., Knapp, S., A systematic interaction map of validated kinase inhibitors with Ser/Thr kinases (2007) Proc. Natl. Acad. Sci. USA, 104, pp. 20523-20528Roberge, M., Berlinck, R.G., Xu, L., Anderson, H.J., Lim, L.Y., Curman, D., Stringer, C.M., Vincent, I., High-throughput assay for G2 checkpoint inhibitors and identification of the structurally novel compound isogranulatimide (1998) Cancer Res, 58, pp. 5701-5706Jiang, X., Zhao, B., Britton, R., Lim, L.Y., Leong, D., Sanghera, J.S., Zhou, B.B., Roberge, M., Inhibition of Chk1 by the G2 DNA damage checkpoint inhibitor isogranulatimide (2004) Mol. Cancer Ther., 3, pp. 1221-1227De Castro, E., Sigrist, C.J.A., Gattiker, A., Bulliard, V., Langendijk-Genevaux, P.S., Gasteiger, E., Bairoch, A., Hulo, N., ScanProsite: Detection of PROSITE signature matches and ProRule-associated functional and structural residues in proteins (2006) Nucleic Acids Res., 34, pp. W362-W365Gavrin, L.K., Saiah, E., Approaches to discover non-ATP site kinase inhibitors (2013) Med. Chem. Commun., 4, pp. 41-51Oliveira, S.H., Ferraz, F.A., Honorato, R.V., Xavier-Neto, J., Sobreira, T.J., De Oliveira, P.S., KVFinder: Steered identification of protein cavities as a PyMOL plugin (2014) BMC Bioinform., 15, p. 197Meirelles, G.V., Lanza, D.C.F., Silva, J.C., Bernachi, J.S., Leme, A.F.P., Kobarg, J., Characterization of hNek6 Interactome Reveals an Important Role for Its Short N-Terminal Domain and Colocalization with Proteins at the Centrosome (2010) J. Proteome Res., 9, pp. 6298-6316Trott, O., Olson, A.J., AutoDockVina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization and multithreading (2010) J. Comput. Chem., 31, pp. 455-46

Validação Da Versão De 36 Itens Do Who Disability Assessment Schedule 2.0 (whodas 2.0) Para A Avaliação De Incapacidade E Funcionalidade Da Mulher Associada à Morbidade Materna

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)To validate the translation and adaptation to Brazilian Portuguese of 36 items from the World Health Organizaton Disability Assessment Schedule 2.0 (WHODAS 2.0), regarding their content and structure (construct), in a female population after pregnancy. Methods This is a validation of an instrument for the evaluation of disability and functioning and an assessment of its psychometric properties, performed in a tertiary maternity and a referral center specialized in high-risk pregnancies in Brazil. A sample of 638 women in different postpartum periods who had either a normal or a complicated pregnancy was included. The structure was evaluated by exploratory factor analysis (EFA) and confirmatory factor analysis (CFA), while the content and relationships among the domains were assessed through Pearson’s correlation coefficient. The sociodemographic characteristics were identified, and the mean scores with their standard deviations for the 36 questions of the WHODAS 2.0 were calculated. The internal consistency was evaluated byCronbach’s α. Results Cronbach’s α was higher than 0.79 for both sets of questons of the questionnaire. The EFA and CFA for the main 32 questions exhibited a total variance of 54.7% (Kaiser-Meyer-Olkin [KMO] measure of sampling adequacy = 0.934; p < 0.001) and 53.47% (KMO = 0.934; p < 0.001) respectively. There was a significant correlation among the 6 domains (r = 0.571–0.876), and a moderate correlation among all domains (r = 0.476–0.694). Conclusion The version of the WHODAS 2.0 instrument adapted to Brazilian Portuguese showed good psychometric properties in this sample, and therefore could be applied to populations of women regarding their reproductive history. © 2017 by Thieme-Revinter Publicações Ltda, Rio de Janeiro, Brazil.3924452471142/2011-5, CNPq, Conselho Nacional de Desenvolvimento Científico e TecnológicoConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq