Plan de negocio para determinar la viabilidad de la implementaci?n de una plataforma digital educativa de cursos de inversiones para la empresa Washington Capital

El pa?s viene incrementando, cada a?o, el crecimiento econ?mico, as? como la cultura digital. Todo esto acompa?ado por la aparici?n de nuevas tecnolog?as que son r?pidamente aceptadas por el consumidor, que busca cada vez m?s, alternativas de consumo que puedan adaptarse a su nuevo estilo de vida agitado, que le permitan comodidad y ahorro de tiempo. Es en este escenario que el presente plan de negocio analiza la factibilidad de desarrollar una unidad de negocio de cursos online para la empresa Washington Capital. En el presente estudio se realiza un an?lisis detallado del entorno, a fin de identificar aquellos factores u oportunidades que permitir?n alcanzar el ?xito de la propuesta de negocio y una minuciosa investigaci?n del mercado para determinar los principales insights, percepciones y preferencias de los consumidores. Con esta informaci?n se procede al dise?o de producto y las estrategias del marketing mix. Finalmente se realiza la evaluaci?n financiera para determinar la factibilidad econ?mica de la unidad de negocio

cIMPACT-NOW Update 9: Recommendations on Utilization of Genome-Wide DNA Methylation Profiling for Central Nervous System Tumor Diagnostics

Genome-wide DNA methylation signatures correlate with and distinguish central nervous system (CNS) tumor types. Since the publication of the initial CNS tumor DNA methylation classifier in 2018, this platform has been increasingly used as a diagnostic tool for CNS tumors, with multiple studies showing the value and utility of DNA methylation – based classification of CNS tumors. A Consortium to Inform Molecular and Practical Approaches to CNS Tumor Taxonomy (cIMPACT-NOW) Working Group was therefore convened to describe the current state of the field and to provide advice based on lessons learned to date. Here, we provide recommendations for the use of DNA methylation-based classification in CNS tumor diagnostics, emphasizing attributes and limitations of the modality. We emphasize that methylation classifier is one diagnostic tool to be used alongside previously established diagnostic tools in a fully integrated fashion. In addition, we provide examples of the inclusion of DNA methylation data within the layered diagnostic reporting format endorsed by the World Health Organization (WHO) and the International Collaboration on Cancer Reporting. We emphasize the need for backwards compatibility of future platforms to enable accumulated data to be compatible with new versions of the array. Finally, we outline the specific connections between methylation classes and CNS WHO tumor types to aid in the interpretation of classifier results. It is hoped that this update will assist the neuro-oncology community in the interpretation of DNA methylation classifier results to facilitate the accurate diagnosis of CNS tumors and thereby help guide patient management

Recherche

ARTIKELEN: C.J. de Poot - Dilemma's in de opsporing E.J. van der Torre en E.R. Muller - Het recherchevak; een institutionele benadering C.J.de Poot en P.J. van Koppen - Meten van recherchewerk F. van Tulder, P. Smit en S. Siero - Ophelderingspercentages als maatsaf voor prestaties? B.L. Mac Lean - Contact tussen O.M. en recherche door de jaren heen; de praktijk A.G. van Amelsvoort, H. Groenendaal en J. van Manen - Werkwijze bij het onderzoek op de Plaats Delict (PD) R.J. Bokhorst - De telefoontap in grote opsporingsonderzoeken 8. J. van der Schoor - Brains voor de recherche SAMENVATTING: In dit themanummer wordt een beeld geschetst van het veranderingsproces dat de recherche doormaakt. Er is voor gekozen  zowel wetenschappers als praktijkdeskundigen aan het woord te laten over hun visies op de organisatie en het dagelijks werk van de recherche

Functional Organization of Locomotor Interneurons in the Ventral Lumbar Spinal Cord of the Newborn Rat

Although the mammalian locomotor CPG has been localized to the lumbar spinal cord, the functional-anatomical organization of flexor and extensor interneurons has not been characterized. Here, we tested the hypothesis that flexor and extensor interneuronal networks for walking are physically segregated in the lumbar spinal cord. For this purpose, we performed optical recordings and lesion experiments from a horizontally sectioned lumbar spinal cord isolated from neonate rats. This ventral hemi spinal cord preparation produces well-organized fictive locomotion when superfused with 5-HT/NMDA. The dorsal surface of the preparation was visualized using the Ca2+ indicator fluo-4 AM, while simultaneously monitoring motor output at ventral roots L2 and L5. Using calcium imaging, we provided a general mapping view of the interneurons that maintained a stable phase relationship with motor output. We showed that the dorsal surface of L1 segment contains a higher density of locomotor rhythmic cells than the other segments. Moreover, L1 segment lesioning induced the most important changes in the locomotor activity in comparison with lesions at the T13 or L2 segments. However, no lesions led to selective disruption of either flexor or extensor output. In addition, this study found no evidence of functional parcellation of locomotor interneurons into flexor and extensor pools at the dorsal-ventral midline of the lumbar spinal cord of the rat

Imported malaria in a cosmopolitan European city: A mirror image of the world epidemiological situation

© 2008 Millet et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution Licens

Whole-genome sequencing reveals host factors underlying critical COVID-19

Critical COVID-19 is caused by immune-mediated inflammatory lung injury. Host genetic variation influences the development of illness requiring critical care1 or hospitalization2–4 after infection with SARS-CoV-2. The GenOMICC (Genetics of Mortality in Critical Care) study enables the comparison of genomes from individuals who are critically ill with those of population controls to find underlying disease mechanisms. Here we use whole-genome sequencing in 7,491 critically ill individuals compared with 48,400 controls to discover and replicate 23 independent variants that significantly predispose to critical COVID-19. We identify 16 new independent associations, including variants within genes that are involved in interferon signalling (IL10RB and PLSCR1), leucocyte differentiation (BCL11A) and blood-type antigen secretor status (FUT2). Using transcriptome-wide association and colocalization to infer the effect of gene expression on disease severity, we find evidence that implicates multiple genes—including reduced expression of a membrane flippase (ATP11A), and increased expression of a mucin (MUC1)—in critical disease. Mendelian randomization provides evidence in support of causal roles for myeloid cell adhesion molecules (SELE, ICAM5 and CD209) and the coagulation factor F8, all of which are potentially druggable targets. Our results are broadly consistent with a multi-component model of COVID-19 pathophysiology, in which at least two distinct mechanisms can predispose to life-threatening disease: failure to control viral replication; or an enhanced tendency towards pulmonary inflammation and intravascular coagulation. We show that comparison between cases of critical illness and population controls is highly efficient for the detection of therapeutically relevant mechanisms of disease

Conforto térmico e desempenho de bezerras Girolando alojadas em abrigos individuais com diferentes coberturas

Esta pesquisa foi desenvolvida com o objetivo de caracterizar os efeitos do ambiente térmico nos índices de conforto, respostas fisiológicas e no desempenho de bezerras Girolando, alojadas em abrigos individuais cobertos com diferentes materiais. O experimento foi realizado entre janeiro e março de 2012, com duração de 56 dias, conduzido com 24 bezerras de composição genética 7/8 Holandês-Gir aos 15 dias de vida e com peso médio de 40,6 kg. Os tratamentos consistiram em três tipos de cobertura, palha de palmeira, telha de polímero reciclado e telha de fibrocimento, com 8 repetições. O delineamento experimental adotado foi o inteiramente casualizado com comparação entre médias pelo teste de Tukey (p < 0,05). A entalpia e a carga térmica radiante diferiram estatisticamente em todos os tratamentos sendo os menores valores apresentados pelos abrigos com cobertura de telha reciclada, 59,3 kJ kg-1 de ar seco e 444,8 W m-2, respectivamente. Não houve diferença significativa em nenhuma das variáveis fisiológicas estudadas, porém a frequência respiratória esteve elevada em todos os tratamentos sendo mais acentuada nos animais sob cobertura de fibrocimento (57,2 mov min-1) indicando mais suscetibilidade ao estresse térmico