Isolation and characterization of a human cementocyte-like cell line, HCY-23

Cementum is the mineralized tissue covering the tooth root that functions in tooth attachment and post-eruptive adjustment of tooth position. It has been reported to be highly similar to bone in several respects but remains poorly understood in terms of development and regeneration. Here, we investigate whether cementocytes, the residing cells in cellular cementum, have the potential to be protagonist in cementum homeostasis, responding to endocrine signals and directing local cementum metabolism. Cells from healthy erupted human teeth were isolated using sequential collagenase/EDTA digestions, and maintained in standard cell culture conditions. A cementocyte-like cell line was cloned (HCY-23, for human cementocyte clone 23), which presented a cementocyte compatible gene expression signature, including the expression of dentin matrix protein 1 ( DMP1 ), sclerostin ( SOST ), and E11/gp38/podoplanin ( E11 ). In contrast, these cells did not express the odontoblast/dentin marker dentin sialoprotein ( DSPP ). HCY-23 cells produced mineral-like nodules in vitro under differentiation conditions, and were highly responsive to inorganic phosphate (Pi). Within the limits of the present study, it can be concluded that cementocytes are phosphate-responsive cells, and have the potential do play a key role in periodontal homeostasis and regeneration33CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP304680/2014-12015/07232-0; 2015/06372-

Gravitino Dark Matter in the CMSSM and Implications for Leptogenesis and the LHC

In the framework of the CMSSM we study the gravitino as the lightest supersymmetric particle and the dominant component of cold dark matter in the Universe. We include both a thermal contribution to its relic abundance from scatterings in the plasma and a non--thermal one from neutralino or stau decays after freeze--out. In general both contributions can be important, although in different regions of the parameter space. We further include constraints from BBN on electromagnetic and hadronic showers, from the CMB blackbody spectrum and from collider and non--collider SUSY searches. The region where the neutralino is the next--to--lightest superpartner is severely constrained by a conservative bound from excessive electromagnetic showers and probably basically excluded by the bound from hadronic showers, while the stau case remains mostly allowed. In both regions the constraint from CMB is often important or even dominant. In the stau case, for the assumed reasonable ranges of soft SUSY breaking parameters, we find regions where the gravitino abundance is in agreement with the range inferred from CMB studies, provided that, in many cases, a reheating temperature \treh is large, \treh\sim10^{9}\gev. On the other side, we find an upper bound \treh\lsim 5\times 10^{9}\gev. Less conservative bounds from BBN or an improvement in measuring the CMB spectrum would provide a dramatic squeeze on the whole scenario, in particular it would strongly disfavor the largest values of \treh\sim 10^{9}\gev. The regions favored by the gravitino dark matter scenario are very different from standard regions corresponding to the neutralino dark matter, and will be partly probed at the LHC.Comment: JHEP version, several improvements and update

Distinguishing circumstellar from stellar photometric variability in Eta Carinae

The interacting binary Eta Carinae remains one of the most enigmatic massive stars in our Galaxy despite over four centuries of observations. In this work, its light curve from the ultraviolet to the near-infrared is analysed using spatially resolved HST observations and intense monitoring at the La Plata Observatory, combined with previously published photometry. We have developed a method to separate the central stellar object in the ground-based images using HST photometry and applying it to the more numerous ground-based data, which supports the hypothesis that the central source is brightening faster than the almost-constant Homunculus. After detrending from long-term brightening, the light curve shows periodic orbital modulation (V ∼ 0.6 mag) attributed to the wind–wind collision cavity as it sweeps around the primary star and it shows variable projected area to our line-of-sight. Two quasi-periodic components with time-scales of 2–3 and 8–10 yr and low amplitude, V < 0.2 mag, are superimposed on the brightening light curve, being the only stellar component of variability found, which indicates minimal stellar instability. Moreover, the light-curve analysis shows no evidence of ‘shell ejections’ at periastron. We propose that the long-term brightening of the stellar core is due to the dissipation of a dusty clump in front of the central star, which works like a natural coronagraph. Thus, the central stars appear to be more stable than previously thought since the dominant variability originates from a changing circumstellar medium. We predict that the brightening phase, due mainly to dust dissipation, will be completed around 2032 ± 4 yr, when the star will be brighter than in the 1600s by up to V ∼ 1 mag.Fil: Damineli, A.. Universidade do Sao Paulo. Instituto de Astronomia, Geofísica e Ciências Atmosféricas; BrasilFil: Fernandez Lajus, Eduardo Eusebio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; ArgentinaFil: Almeida, L.A.. Universidade do Sao Paulo. Instituto de Astronomia, Geofísica e Ciências Atmosféricas; Brasil. Universidade Federal do Rio Grande do Norte; BrasilFil: Corcoran, M.F.. National Aeronautics and Space Administration; Estados Unidos. The Catholic University of America; Estados UnidosFil: Damineli, D.S.C.. University of Maryland; Estados UnidosFil: Gull, T.R.. National Aeronautics and Space Administration; Estados UnidosFil: Hamaguchi, K. National Aeronautics and Space Administration; Estados Unidos. University of Maryland; Estados UnidosFil: Hillier, D.J.. University of Pittsburgh; Estados UnidosFil: Jablonski, F.J.. Centro de Previsao de Tempo e Estudos Climáticos. Instituto Nacional de Pesquisas Espaciais; BrasilFil: Madura, T.I.. San Jose State University; Estados UnidosFil: Moffat, A.F.J.. Université du Québec a Montreal; CanadáFil: Navarete, F.. Universidade do Sao Paulo. Instituto de Astronomia, Geofísica e Ciências Atmosféricas; BrasilFil: Richardson, N.D.. University Of Toledo (utoledo); Estados UnidosFil: Ruiz, G.F.. Universidade do Sao Paulo. Instituto de Astronomia, Geofísica e Ciências Atmosféricas; BrasilFil: Salerno, N.E.. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas; ArgentinaFil: Scalia, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; ArgentinaFil: Weigelt, G.. Max Planck Institute For Radio Astronomy; Alemani

Search for displaced vertices arising from decays of new heavy particles in 7 TeV pp collisions at ATLAS

We present the results of a search for new, heavy particles that decay at a significant distance from their production point into a final state containing charged hadrons in association with a high-momentum muon. The search is conducted in a pp-collision data sample with a center-of-mass energy of 7 TeV and an integrated luminosity of 33 pb^-1 collected in 2010 by the ATLAS detector operating at the Large Hadron Collider. Production of such particles is expected in various scenarios of physics beyond the standard model. We observe no signal and place limits on the production cross-section of supersymmetric particles in an R-parity-violating scenario as a function of the neutralino lifetime. Limits are presented for different squark and neutralino masses, enabling extension of the limits to a variety of other models.Comment: 8 pages plus author list (20 pages total), 8 figures, 1 table, final version to appear in Physics Letters

Measurement of the polarisation of W bosons produced with large transverse momentum in pp collisions at sqrt(s) = 7 TeV with the ATLAS experiment

This paper describes an analysis of the angular distribution of W->enu and W->munu decays, using data from pp collisions at sqrt(s) = 7 TeV recorded with the ATLAS detector at the LHC in 2010, corresponding to an integrated luminosity of about 35 pb^-1. Using the decay lepton transverse momentum and the missing transverse energy, the W decay angular distribution projected onto the transverse plane is obtained and analysed in terms of helicity fractions f0, fL and fR over two ranges of W transverse momentum (ptw): 35 < ptw < 50 GeV and ptw > 50 GeV. Good agreement is found with theoretical predictions. For ptw > 50 GeV, the values of f0 and fL-fR, averaged over charge and lepton flavour, are measured to be : f0 = 0.127 +/- 0.030 +/- 0.108 and fL-fR = 0.252 +/- 0.017 +/- 0.030, where the first uncertainties are statistical, and the second include all systematic effects.Comment: 19 pages plus author list (34 pages total), 9 figures, 11 tables, revised author list, matches European Journal of Physics C versio

Observation of a new chi_b state in radiative transitions to Upsilon(1S) and Upsilon(2S) at ATLAS

The chi_b(nP) quarkonium states are produced in proton-proton collisions at the Large Hadron Collider (LHC) at sqrt(s) = 7 TeV and recorded by the ATLAS detector. Using a data sample corresponding to an integrated luminosity of 4.4 fb^-1, these states are reconstructed through their radiative decays to Upsilon(1S,2S) with Upsilon->mu+mu-. In addition to the mass peaks corresponding to the decay modes chi_b(1P,2P)->Upsilon(1S)gamma, a new structure centered at a mass of 10.530+/-0.005 (stat.)+/-0.009 (syst.) GeV is also observed, in both the Upsilon(1S)gamma and Upsilon(2S)gamma decay modes. This is interpreted as the chi_b(3P) system.Comment: 5 pages plus author list (18 pages total), 2 figures, 1 table, corrected author list, matches final version in Physical Review Letter

Measurement of the inclusive isolated prompt photon cross-section in pp collisions at sqrt(s)= 7 TeV using 35 pb-1 of ATLAS data

A measurement of the differential cross-section for the inclusive production of isolated prompt photons in pp collisions at a center-of-mass energy sqrt(s) = 7 TeV is presented. The measurement covers the pseudorapidity ranges |eta|<1.37 and 1.52<=|eta|<2.37 in the transverse energy range 45<=E_T<400GeV. The results are based on an integrated luminosity of 35 pb-1, collected with the ATLAS detector at the LHC. The yields of the signal photons are measured using a data-driven technique, based on the observed distribution of the hadronic energy in a narrow cone around the photon candidate and the photon selection criteria. The results are compared with next-to-leading order perturbative QCD calculations and found to be in good agreement over four orders of magnitude in cross-section.Comment: 7 pages plus author list (18 pages total), 2 figures, 4 tables, final version published in Physics Letters

Search for direct production of charginos and neutralinos in events with three leptons and missing transverse momentum in √s = 7 TeV pp collisions with the ATLAS detector

A search for the direct production of charginos and neutralinos in final states with three electrons or muons and missing transverse momentum is presented. The analysis is based on 4.7 fb−1 of proton–proton collision data delivered by the Large Hadron Collider and recorded with the ATLAS detector. Observations are consistent with Standard Model expectations in three signal regions that are either depleted or enriched in Z-boson decays. Upper limits at 95% confidence level are set in R-parity conserving phenomenological minimal supersymmetric models and in simplified models, significantly extending previous results