Tomato: a crop species amenable to improvement by cellular and molecular methods

Tomato is a crop plant with a relatively small DNA content per haploid genome and a well developed genetics. Plant regeneration from explants and protoplasts is feasable which led to the development of efficient transformation procedures. In view of the current data, the isolation of useful mutants at the cellular level probably will be of limited value in the genetic improvement of tomato. Protoplast fusion may lead to novel combinations of organelle and nuclear DNA (cybrids), whereas this technique also provides a means of introducing genetic information from alien species into tomato. Important developments have come from molecular approaches. Following the construction of an RFLP map, these RFLP markers can be used in tomato to tag quantitative traits bred in from related species. Both RFLP's and transposons are in the process of being used to clone desired genes for which no gene products are known. Cloned genes can be introduced and potentially improve specific properties of tomato especially those controlled by single genes. Recent results suggest that, in principle, phenotypic mutants can be created for cloned and characterized genes and will prove their value in further improving the cultivated tomato.

An Integrated TCGA Pan-Cancer Clinical Data Resource to Drive High-Quality Survival Outcome Analytics

For a decade, The Cancer Genome Atlas (TCGA) program collected clinicopathologic annotation data along with multi-platform molecular profiles of more than 11,000 human tumors across 33 different cancer types. TCGA clinical data contain key features representing the democratized nature of the data collection process. To ensure proper use of this large clinical dataset associated with genomic features, we developed a standardized dataset named the TCGA Pan-Cancer Clinical Data Resource (TCGA-CDR), which includes four major clinical outcome endpoints. In addition to detailing major challenges and statistical limitations encountered during the effort of integrating the acquired clinical data, we present a summary that includes endpoint usage recommendations for each cancer type. These TCGA-CDR findings appear to be consistent with cancer genomics studies independent of the TCGA effort and provide opportunities for investigating cancer biology using clinical correlates at an unprecedented scale. Analysis of clinicopathologic annotations for over 11,000 cancer patients in the TCGA program leads to the generation of TCGA Clinical Data Resource, which provides recommendations of clinical outcome endpoint usage for 33 cancer types

Measurement of the mass difference between top quark and antiquark in pp collisions at root s=8 TeV

Peer reviewe

Meditation-induced bliss viewed as release from conditioned neural (thought) patterns that block reward signals in the brain pleasure center

The nucleus accumbens orchestrates processes related to reward and pleasure, including the addictive consequences of repeated reward (e.g., drug addiction and compulsive gambling) and the accompanying feelings of craving and anhedonia. The neurotransmitters dopamine and endogenous opiates play interactive roles in these processes. They are released by natural rewards (i.e., food, water, sex, money, play, etc.) and are released or mimicked by drugs of abuse. Repeated drug use induces conditioned down-regulation of these neurotransmitters, thus causing painful suppression of everyday pleasure. As with many spiritual traditions, Buddhism provides strong advice against the pursuit of worldly pleasures to attain the ‘‘good life.’’ In contrast, many forms of meditation give rise to an immense and abiding joy. Most of these practices involve ‘‘stilling the mind,’’ whereby all content-laden thought (e.g., fantasies, daydreams, plans) ceases, and the mind enters a state of openness, formlessness, clarity, and bliss. This can be explained by the Buddhist suggestion that almost all of our everyday thoughts are a form of addiction. It follows that if we turn off this internal ‘‘gossip of ego,’’ we will find relief from the biochemical dopamine/opiate down-regulation, which is, perhaps, the perpetual concomitant of our daily rumination

Evolution of single-particle structure near the N=20 island of inversion

7 pags., 5 figs., 2 tabs.The single-particle properties of Mg29 have been investigated via a measurement of the Mg28(d,p)Mg29 reaction, in inverse kinematics, using the ISOLDE Solenoidal Spectrometer. The negative-parity intruder states from the fp shell have been identified and used to benchmark modern shell-model calculations. The systematic data on the single-particle centroids along the N=17 isotones show good agreement with shell-model predictions in describing the observed trends from stability toward O25. However, there is also evidence that the effect of the finite geometry of the nuclear potential is playing a role on the behavior of the p orbitals near the particle-emission threshold.This work wassupported by the U.K. Science and Technology Facilities Council [Grants No. ST/P004598/1, No. ST/N002563/1, No. ST/M00161X/1 (Liverpool), No. ST/P004423/1 (Manchester), No. ST/P005314/1 (Surrey), the ISOL-SRS Grant (Daresbury), No. ST/R004056/1 (Ernest Rutherford Fellowship - Gaffney), and No. ST/T004797/1 (Ernest Rutherford Fellowship - Sharp)], the U.S. Department of Energy, Office of Science, Office of Nuclear Physics, under Contracts No. DE-AC02-06CH11357 (ANL) and No. DE-SC-0014552 (UConn), the European Union’s Horizon 2020 Framework research and innovation program under Grant Agreement No. 654002 (ENSAR2), the Marie Skłodowska-Curie Grant Agreement No. 665779, the Research Foundation Flanders (FWO, Belgium), the European Research Council under the European Union’s Seventh Framework Programme (FP7/2007-2013)/ERC Grant Agreement No. 617156, and the Spanish Ministry of Science and Innovation under Grants No. PGC2018-095640- B-I00“ELEGANT” and No. PID2019-104390GB-I00. This research used targets provided by the Center for Accelerator Target Science at Argonne National Laboratory. The FSU shell-model calculations were performed using the computational facility of the nuclear physics theory group, Florida State University, supported by grants from the U.S. Department of Energy, Office of Science (DE-SC-0009883 (FSU)

Evolution of the nuclear spin-orbit splitting explored via the ³²Si<i>(d,p)</i>³³Si reaction using SOLARIS

The spin-orbit splitting between neutron 1p orbitals at 33Si has been deduced using the single-neutron-adding (d,p) reaction in inverse kinematics with a beam of 32Si, a long-lived radioisotope. Reaction products were analyzed by the newly implemented SOLARIS spectrometer at the reaccelerated-beam facility at the National Superconducting Cyclotron Laboratory. The measurements show reasonable agreement with shell-model calculations that incorporate modern cross-shell interactions, but they contradict the prediction of proton density depletion based on relativistic mean-field theory. The evolution of the neutron 1p-shell orbitals is systematically studied using the present and existing data in the isotonic chains of = 17, 19, and 21. In each case, a smooth decrease in the separation of the - orbitals is seen as the respective p-orbitals approach zero binding, suggesting that the finite nuclear potential strongly influences the evolution of nuclear structure in this region