Far infrared observations of pre-protostellar sources in Lynds 183

Using ISOPHOT maps at 100 and 200um and raster scans at 100, 120, 150 and 200um we have detected four unresolved far-infrared sources in the high latitude molecular cloud L183. Two of the sources are identified with 1.3mm continuum sources found by Ward-Thompson et al. and are located near the temperature minimum and the coincident column density maximum of dust distribution. For these two sources, the ISO observations have enabled us to derive temperatures (about 8.3 K) and masses (about 1.4 and 2.4 solar masses). They are found to have masses greater than or comparable to their virial masses and are thus expected to undergo gravitational collapse. We classify them as pre-protostellar sources. The two new sources are good candidates for pre-protostellar sources or protostars within L183.Comment: 12 pages, 7 Postscript figures, 1 JPEG figure. Accepted for publication in Astronomy & Astrophysic

Spitzer characterisation of dust in an anomalous emission region: the Perseus cloud

Anomalous microwave emission is known to exist in the Perseus cloud. One of the most promising candidates to explain this excess of emission is electric dipole radiation from rapidly rotating very small dust grains, commonly referred to as spinning dust. Photometric data obtained with the Spitzer Space Telescope have been reprocessed and used in conjunction with the dust emission model DUSTEM to characterise the properties of the dust within the cloud. This analysis has allowed us to constrain spatial variations in the strength of the interstellar radiation field ($\chi_\mathrm{ISRF}$), the mass abundances of the PAHs and VSGs relative to the BGs (Y$_\mathrm{PAH}$ and Y$_\mathrm{VSG}$), the column density of hydrogen (N$_\mathrm{H}$) and the equilibrium dust temperature (T$_\mathrm{dust}$). The parameter maps of Y$_\mathrm{PAH}$, Y$_\mathrm{VSG}$ and $\chi_\mathrm{ISRF}$ are the first of their kind to be produced for the Perseus cloud, and we used these maps to investigate the physical conditions in which anomalous emission is observed. We find that in regions of anomalous emission the strength of the ISRF, and consequently the equilibrium temperature of the dust, is enhanced while there is no significant variation in the abundances of the PAHs and the VSGs or the column density of hydrogen. We interpret these results as an indication that the enhancement in $\chi_\mathrm{ISRF}$ might be affecting the properties of the small stochastically heated dust grains resulting in an increase in the spinning dust emission observed at 33 GHz. This is the first time that such an investigation has been performed, and we believe that this type of analysis creates a new perspective in the field of anomalous emission studies, and represents a powerful new tool for constraining spinning dust models.Comment: 13 pages, 9 figures, accepted for publication in MNRA

A Broadband Study of Galactic Dust Emission

We have combined infrared data with HI, H2 and HII surveys in order to spatially decompose the observed dust emission into components associated with different phases of the gas. An inversion technique is applied. For the decomposition, we use the IRAS 60 and 100 micron bands, the DIRBE 140 and 240 micron bands, as well as Archeops 850 and 2096 micron wavelengths. In addition, we apply the decomposition to all five WMAP bands. We obtain longitude and latitude profiles for each wavelength and for each gas component in carefully selected Galactic radius bins.We also derive emissivity coefficients for dust in atomic, molecular and ionized gas in each of the bins.The HI emissivity appears to decrease with increasing Galactic radius indicating that dust associated with atomic gas is heated by the ambient interstellar radiation field (ISRF). By contrast, we find evidence that dust mixed with molecular clouds is significantly heated by O/B stars still embedded in their progenitor clouds. By assuming a modified black-body with emissivity law lambda^(-1.5), we also derive the radial distribution of temperature for each phase of the gas. All of the WMAP bands except W appear to be dominated by emission from something other than normal dust, most likely a mixture of thermal bremstrahlung from diffuse ionized gas, synchrotron emission and spinning dust. Furthermore, we find indications of an emissivity excess at long wavelengths (lambda > 850 micron) in the outer Galaxy (R > 8.9 kpc). This suggests either the existence of a very cold dust component in the outer Galaxy or a temperature dependence of the spectral emissivity index. Finally, it is shown that ~ 80% of the total FIR luminosity is produced by dust associated with atomic hydrogen, in agreement with earlier findings by Sodroski et al. (1997).Comment: accepted for publication by A&

Physical structure of the photodissociation regions in NGC 7023: Observations of gas and dust emission with <i>Herschel</i>

The determination of the physical conditions in molecular clouds is a key step towards our understanding of their formation and evolution of associated star formation. We investigate the density, temperature, and column density of both dust and gas in the photodissociation regions (PDRs) located at the interface between the atomic and cold molecular gas of the NGC 7023 reflection nebula. We study how young stars affect the gas and dust in their environment. Our approach combining both dust and gas delivers strong constraints on the physical conditions of the PDRs. We find dense and warm molecular gas of high column density in the PDRs

Velocity field and star formation in the Horsehead nebula

Using large scale maps in C18O(2-1) and in the continuum at 1.2mm obtained at the IRAM-30m antenna with the Heterodyne Receiver Array (HERA) and MAMBO2, we investigated the morphology and the velocity field probed in the inner layers of the Horsehead nebula. The data reveal a non--self-gravitating (m/mvir = 0.3) filament of dust and gas (the "neck", diameter = 0.15-0.30 pc) connecting the Horsehead western ridge, a Photon-Dominated Region illuminated by sigmaOri, to its parental cloud L1630. Several dense cores are embedded in the ridge and the neck. One of these cores appears particularly peaked in the 1.2 mm continuum map and corresponds to a feature seen in absorption on ISO maps around 7 micr. Its \cdo emission drops at the continuum peak, suggestive of molecular depletion onto cold grains. The channel maps of the Horsehead exhibit an overall north-east velocity gradient whose orientation swivels east-west, showing a somewhat more complex structure than was recently reported by \cite{pound03} using BIMA CO(1-0) mapping. In both the neck and the western ridge, the material is rotating around an axis extending from the PDR to L1630 (angular velocity=1.5-4.0 km/s). Moreover, velocity gradients along the filament appear to change sign regularly (3 km/s/pc, period=0.30 pc) at the locations of embedded integrated intensity peaks. The nodes of this oscillation are at the same velocity. Similar transverse cuts across the filament show a sharp variation of the angular velocity in the area of the main dense core. The data also suggest that differential rotation is occurring in parts of the filament. We present a new scenario for the formation and evolution of the nebula and discuss dense core formation inside the filament.Comment: 11 pages, 9 figures, A&

Mapping the cold dust temperatures and masses of nearby Kingfish galaxies with Herschel

Taking advantage of the sensitivity and angular resolution of the Herschel Space Observatory at far-infrared and submm wavelengths, we aim to characterize the physical properties of cold dust within nearby galaxies and study the robustness of the parameters we derive using different modified blackbody models. For a pilot subsample of the KINGFISH program, we perform 2 temperature fits of the Spitzer and Herschel photometric data (24 to 500um), with a warm and a cold component, globally and in each resolution element.At global scales, we observe ranges of values for beta_c(0.8 to 2.5) and Tc(19.1 to 25.1K).We compute maps of our parameters with beta fixed or free to test the robustness of the temperature and dust surface density maps we deduce. When the emissivity is fixed, we observe temperature gradients as a function of radius.When the emissivity is fitted as a free parameter, barred galaxies tend to have uniform fitted emissivities.Gathering resolved elements in a Tc-beta_c diagram underlines an anti-correlation between the two parameters.It remains difficult to assess whether the dominant effect is the physics of dust grains, noise, or mixing along the line of sight and in the beam. We finally observe in both cases that the dust column density peaks in central regions of galaxies and bar ends (coinciding with molecular gas density enhancements usually found in these locations).We also quantify how the total dust mass varies with our assumptions about the emissivity index as well as the influence of the wavelength coverage used in the fits. We show that modified blackbody fits using a shallow emissivity (beta_c < 2.0) lead to significantly lower dust masses compared to the beta_c < 2.0 case, with dust masses lower by up to 50% if beta_c=1.5 for instance.The working resolution affects our total dust mass estimates: masses increase from global fits to spatially-resolved fits.Comment: 26 pages, 12 figures, 4 tables, accepted for publication in MNRAS, 2012 June 2

An ECVAG† trial on assessment of oxidative damage to DNA measured by the comet assay

The increasing use of single cell gel electrophoresis (the comet assay) highlights its popularity as a method for detecting DNA damage, including the use of enzymes for assessment of oxidatively damaged DNA. However, comparison of DNA damage levels between laboratories can be difficult due to differences in assay protocols (e.g. lysis conditions, enzyme treatment, the duration of the alkaline treatment and electrophoresis) and in the end points used for reporting results (e.g. %DNA in tail, arbitrary units, tail moment and tail length). One way to facilitate comparisons is to convert primary comet assay end points to number of lesions/106 bp by calibration with ionizing radiation. The aim of this study was to investigate the inter-laboratory variation in assessment of oxidatively damaged DNA by the comet assay in terms of oxidized purines converted to strand breaks with formamidopyrimidine DNA glycosylase (FPG). Coded samples with DNA oxidation damage induced by treatment with different concentrations of photosensitizer (Ro 19-8022) plus light and calibration samples irradiated with ionizing radiation were distributed to the 10 participating laboratories to measure DNA damage using their own comet assay protocols. Nine of 10 laboratories reported the same ranking of the level of damage in the coded samples. The variation in assessment of oxidatively damaged DNA was largely due to differences in protocols. After conversion of the data to lesions/106 bp using laboratory-specific calibration curves, the variation between the laboratories was reduced. The contribution of the concentration of photosensitizer to the variation in net FPG-sensitive sites increased from 49 to 73%, whereas the inter-laboratory variation decreased. The participating laboratories were successful in finding a dose–response of oxidatively damaged DNA in coded samples, but there remains a need to standardize the protocols to enable direct comparisons between laboratories

The Current Status and Work of Three Rs Centres and Platforms in Europe*

The adoption of Directive 2010/63/EU on the protection of animals used for scientific purposes has given a major push to the formation of Three Rs initiatives in the form of centres and platforms. These centres and platforms are dedicated to the so-called Three Rs, which are the Replacement, Reduction and Refinement of animal use in experiments. ATLA's 50th Anniversary year has seen the publication of two articles on European Three Rs centres and platforms. The first of these was about the progressive rise in their numbers and about their founding history; this second part focuses on their current status and activities. This article takes a closer look at their financial and organisational structures, describes their Three Rs focus and core activities (dissemination, education, implementation, scientific quality/translatability, ethics), and presents their areas of responsibility and projects in detail. This overview of the work and diverse structures of the Three Rs centres and platforms is not only intended to bring them closer to the reader, but also to provide role models and show examples of how such Three Rs centres and platforms could be made sustainable. The Three Rs centres and platforms are very important focal points and play an immense role as facilitators of Directive 2010/63/EU 'on the ground' in their respective countries. They are also invaluable for the wide dissemination of information and for promoting the implementation of the Three Rs in general

The Rise of Three Rs Centres and Platforms in Europe*

Public awareness and discussion about animal experiments and replacement methods has greatly increased in recent years. The term 'the Three Rs', which stands for the Replacement, Reduction and Refinement of animal experiments, is inseparably linked in this context. A common goal within the Three Rs scientific community is to develop predictive non-animal models and to better integrate all available data from in vitro, in silico and omics technologies into regulatory decision-making processes regarding, for example, the toxicity of chemicals, drugs or food ingredients. In addition, it is a general concern to implement (human) non-animal methods in basic research. Toward these efforts, there has been an ever-increasing number of Three Rs centres and platforms established over recent years - not only to develop novel methods, but also to disseminate knowledge and help to implement the Three Rs principles in policies and education. The adoption of Directive 2010/63/EU on the protection of animals used for scientific purposes gave a strong impetus to the creation of Three Rs initiatives, in the form of centres and platforms. As the first of a series of papers, this article gives an overview of the European Three Rs centres and platforms, and their historical development. The subsequent articles, to be published over the course of ATLA's 50th Anniversary year, will summarise the current focus and tasks as well as the future and the plans of the Three Rs centres and platforms. The Three Rs centres and platforms are very important points of contact and play an immense role in their respective countries as 'on the ground' facilitators of Directive 2010/63/EU. They are also invaluable for the widespread dissemination of information and for promoting implementation of the Three Rs in general

Planck 2013 results. XI. All-sky model of thermal dust emission

This paper presents an all-sky model of dust emission from the Planck 353, 545, and 857 GHz, and IRAS 100 \u3bcm data. Using a modified blackbody fit to the data we present all-sky maps of the dust optical depth, temperature, and spectral index over the 353-3000 GHz range. This model is a good representation of the IRAS and Planck data at 5\u2032 between 353 and 3000 GHz (850 and 100 \u3bcm). It shows variations of the order of 30% compared with the widely-used model of Finkbeiner, Davis, and Schlegel. The Planck data allow us to estimate the dust temperature uniformly over the whole sky, down to an angular resolution of 5\u2032, providing an improved estimate of the dust optical depth compared to previous all-sky dust model, especially in high-contrast molecular regions where the dust temperature varies strongly at small scales in response to dust evolution, extinction, and/or local production of heating photons. An increase of the dust opacity at 353 GHz, \u3c4353/NH, from the diffuse to the denser interstellar medium (ISM) is reported. It is associated with a decrease in the observed dust temperature, Tobs, that could be due at least in part to the increased dust opacity. We also report an excess of dust emission at H i column densities lower than 1020 cm-2 that could be the signature of dust in the warm ionized medium. In the diffuse ISM at high Galactic latitude, we report an anticorrelation between \u3c4353/NH and Tobs while the dust specific luminosity, i.e., the total dust emission integrated over frequency (the radiance) per hydrogen atom, stays about constant, confirming one of the Planck Early Results obtained on selected fields. This effect is compatible with the view that, in the diffuse ISM, Tobs responds to spatial variations of the dust opacity, due to variations of dust properties, in addition to (small) variations of the radiation field strength. The implication is that in the diffuse high-latitude ISM \u3c4353 is not as reliable a tracer of dust column density as we conclude it is in molecular clouds where the correlation of \u3c4353 with dust extinction estimated using colour excess measurements on stars is strong. To estimate Galactic E(B-V) in extragalactic fields at high latitude we develop a new method based on the thermal dust radiance, instead of the dust optical depth, calibrated to E(B-V) using reddening measurements of quasars deduced from Sloan Digital Sky Survey data. \ua9 2014 ESO