Identification of the causal agent of Botryosphaeria stem canker in Ethiopian Eucalyptus plantations

Plantations of exotic Eucalyptus make up more than 30% of Ethiopia's plantations, providing fuel and construction timber to the country. Species such as E. camaldulensis, E. saligna, E. grandis, E. citriodora and E. globulus are most commonly planted. During a survey of Eucalyptus diseases in 2000 and 2001, Botryosphaeria stem canker was observed in most plantations. The disease symptoms included tip die- back, coppice failure and stem cankers characterised by kino exudation. The aim of this study was to identify the species responsible for Botryosphaeria stem canker in Ethiopia. Culture and conidial morphology, as well as DNA-based identification involving Restriction Fragment Length Polymorphisms (RFLPs) and sequencing of the Internal Transcribed Spacer regions (ITS) of the ribosomal RNA gene and the elongation factor 1-alpha (EF1-α) gene, were used to identify isolates. Pathogenicity studies were conducted in the greenhouse and under field conditions. Results showed that Botryosphaeria parva is responsible for Botryosphaeria stem canker of Eucalyptus in Ethiopia. This is the first report of the fungus from this country. Greenhouse and field inoculation studies showed that the Ethiopian isolates are highly virulent. Careful site species selection and breeding trials are thus needed to reduce the impact of this disease in Ethiopia

The production of gibberellin-like substances by Colletotrichum gloeosporioides associated with lupin anthracnose

The most characteristic symptoms of lupin (Lupinus spp.) anthracnose caused by the fungus Colletotrichum gloeosporioides, are twisting and bending of stems and petioles. These might be related to the production of gibberellin-like activity by the pathogen. The barley aleurone bioassay was used to determine whether two isolates of C. gloeosporioides could produce gibberellin-like substances. Ethyl acetate extracts from isolates SHK 2148 and SHK 1033, grown in liquid minimal medium, had gibberellin-like activities of 0.21 μg GA3 equivalents and >0.26μg GA3 equivalents per g dry mass of mycelium, respectively. Provided that the strong association between gibberellins and this specific pathogen/host interaction can be established, this knowledge may be helpful in screening lupin cultivars for anthracnose resistance

Production of triploid Sandersonia aurantiaca plants

Triploid plants of Sandersonia aurantiaca were produced by crossing diploid and tetraploid forms of S. aurantiaca. Enlarged ovules were transferred to in vitro culture 14–30 days after pollination. The triploid nature of the embryo derived plants was determined by flow cytometry and chromosome counts both of which showed that the triploid plants had features that were midway between those of the two parents. The mean nuclear DNA contents of 2C nuclei from diploid, triploid and tetraploid forms of S. aurantiaca were 6.86pg, 10.04pg and 13.55pg, respectively. The nuclear DNA content of 1C nuclei of sperm cells from pollen grains was 2.94pg. Mitotic chromosome counts from the three plants gave 2n = 24, 36 and 48 chromosomes for the diploid, triploid and tetraploid forms, respectively. Meiotic chromosome counts for the diploid and tetraploid plants were n = 12 and n = 24, respectively. The triploid showed mainly bivalents, but lagging chromosomes led to micronuclei and infertility in gametes. The morphological features of the various plants corroborated other evidence indicating that the triploid plants were the result of a cross between diploid and tetraploid plants

Flavonoids in phylloclades discriminate endemic Semele androgyna chemotypes from Madeira

Thirty-five randomly-collected Semele androgyna Kunth samples were screened by RP-HPLC for their phenolic composition. Fraction analysis allowed the detection of 17 different compounds. According to their retention times and UV spectra obtained by diode array analysis, these phenolics represent three classes: phenolic acids, flavones and flavonols. Co-chromatography with specific standards enabled identification of quercetin, rutin and quercitrin in Semele tissues for the first time. Polymorphism based on phenolic composition was evaluated using multivariate analysis and showed four distinct S. androgyna clusters. This polymorphism was not associated with morphological diversity or different in ambient light intensities. Biochemical differentiation is thus present in this species. The application of multivariate analysis techniques to RP-HPLC data has allowed the classification of samples into two groups, previously proposed on the basis of morphological and cytotaxonomical information. Therefore, the use of phenolics as chemotaxonomic markers in Semele is highly recommended because of its diagnostic value, even at a subspecies level. Discriminant canonical analysis and Mahalanobis distances confirmed these clusters as recognisable chemosystematic units. However, these units do not support the separation of S. pterygophora.The Portuguese Foundation for Science and Technology (FCT) funded this work through the Centre of Macaronesian Studies (CEM). The authors are grateful to the Madeiran Centre of Science and Technology (CITMA), the Berardo Foundation and European Social Funding for financial assistance given during execution of this work. The assistance rendered by Mr Rogério Correia during field collection is gratefully acknowledged.info:eu-repo/semantics/publishedVersio

Search for direct production of charginos and neutralinos in events with three leptons and missing transverse momentum in √s = 7 TeV pp collisions with the ATLAS detector

A search for the direct production of charginos and neutralinos in final states with three electrons or muons and missing transverse momentum is presented. The analysis is based on 4.7 fb−1 of proton–proton collision data delivered by the Large Hadron Collider and recorded with the ATLAS detector. Observations are consistent with Standard Model expectations in three signal regions that are either depleted or enriched in Z-boson decays. Upper limits at 95% confidence level are set in R-parity conserving phenomenological minimal supersymmetric models and in simplified models, significantly extending previous results

Jet size dependence of single jet suppression in lead-lead collisions at sqrt(s(NN)) = 2.76 TeV with the ATLAS detector at the LHC

Measurements of inclusive jet suppression in heavy ion collisions at the LHC provide direct sensitivity to the physics of jet quenching. In a sample of lead-lead collisions at sqrt(s) = 2.76 TeV corresponding to an integrated luminosity of approximately 7 inverse microbarns, ATLAS has measured jets with a calorimeter over the pseudorapidity interval |eta| < 2.1 and over the transverse momentum range 38 < pT < 210 GeV. Jets were reconstructed using the anti-kt algorithm with values for the distance parameter that determines the nominal jet radius of R = 0.2, 0.3, 0.4 and 0.5. The centrality dependence of the jet yield is characterized by the jet "central-to-peripheral ratio," Rcp. Jet production is found to be suppressed by approximately a factor of two in the 10% most central collisions relative to peripheral collisions. Rcp varies smoothly with centrality as characterized by the number of participating nucleons. The observed suppression is only weakly dependent on jet radius and transverse momentum. These results provide the first direct measurement of inclusive jet suppression in heavy ion collisions and complement previous measurements of dijet transverse energy imbalance at the LHC.Comment: 15 pages plus author list (30 pages total), 8 figures, 2 tables, submitted to Physics Letters B. All figures including auxiliary figures are available at http://atlas.web.cern.ch/Atlas/GROUPS/PHYSICS/PAPERS/HION-2011-02

Measurement of the polarisation of W bosons produced with large transverse momentum in pp collisions at sqrt(s) = 7 TeV with the ATLAS experiment

This paper describes an analysis of the angular distribution of W->enu and W->munu decays, using data from pp collisions at sqrt(s) = 7 TeV recorded with the ATLAS detector at the LHC in 2010, corresponding to an integrated luminosity of about 35 pb^-1. Using the decay lepton transverse momentum and the missing transverse energy, the W decay angular distribution projected onto the transverse plane is obtained and analysed in terms of helicity fractions f0, fL and fR over two ranges of W transverse momentum (ptw): 35 < ptw < 50 GeV and ptw > 50 GeV. Good agreement is found with theoretical predictions. For ptw > 50 GeV, the values of f0 and fL-fR, averaged over charge and lepton flavour, are measured to be : f0 = 0.127 +/- 0.030 +/- 0.108 and fL-fR = 0.252 +/- 0.017 +/- 0.030, where the first uncertainties are statistical, and the second include all systematic effects.Comment: 19 pages plus author list (34 pages total), 9 figures, 11 tables, revised author list, matches European Journal of Physics C versio

Observation of a new chi_b state in radiative transitions to Upsilon(1S) and Upsilon(2S) at ATLAS

The chi_b(nP) quarkonium states are produced in proton-proton collisions at the Large Hadron Collider (LHC) at sqrt(s) = 7 TeV and recorded by the ATLAS detector. Using a data sample corresponding to an integrated luminosity of 4.4 fb^-1, these states are reconstructed through their radiative decays to Upsilon(1S,2S) with Upsilon->mu+mu-. In addition to the mass peaks corresponding to the decay modes chi_b(1P,2P)->Upsilon(1S)gamma, a new structure centered at a mass of 10.530+/-0.005 (stat.)+/-0.009 (syst.) GeV is also observed, in both the Upsilon(1S)gamma and Upsilon(2S)gamma decay modes. This is interpreted as the chi_b(3P) system.Comment: 5 pages plus author list (18 pages total), 2 figures, 1 table, corrected author list, matches final version in Physical Review Letter

Search for displaced vertices arising from decays of new heavy particles in 7 TeV pp collisions at ATLAS

We present the results of a search for new, heavy particles that decay at a significant distance from their production point into a final state containing charged hadrons in association with a high-momentum muon. The search is conducted in a pp-collision data sample with a center-of-mass energy of 7 TeV and an integrated luminosity of 33 pb^-1 collected in 2010 by the ATLAS detector operating at the Large Hadron Collider. Production of such particles is expected in various scenarios of physics beyond the standard model. We observe no signal and place limits on the production cross-section of supersymmetric particles in an R-parity-violating scenario as a function of the neutralino lifetime. Limits are presented for different squark and neutralino masses, enabling extension of the limits to a variety of other models.Comment: 8 pages plus author list (20 pages total), 8 figures, 1 table, final version to appear in Physics Letters