Impact of Different Fecal Processing Methods on Assessments of Bacterial Diversity in the Human Intestine.

The intestinal microbiota are integral to understanding the relationships between nutrition and health. Therefore, fecal sampling and processing protocols for metagenomic surveys should be sufficiently robust, accurate, and reliable to identify the microorganisms present. We investigated the use of different fecal preparation methods on the bacterial community structures identified in human stools. Complete stools were collected from six healthy individuals and processed according to the following methods: (i) randomly sampled fresh stool, (ii) fresh stool homogenized in a blender for 2 min, (iii) randomly sampled frozen stool, and (iv) frozen stool homogenized in a blender for 2 min, or (v) homogenized in a pneumatic mixer for either 10, 20, or 30 min. High-throughput DNA sequencing of the 16S rRNA V4 regions of bacterial community DNA extracted from the stools showed that the fecal microbiota remained distinct between individuals, independent of processing method. Moreover, the different stool preparation approaches did not alter intra-individual bacterial diversity. Distinctions were found at the level of individual taxa, however. Stools that were frozen and then homogenized tended to have higher proportions of Faecalibacterium, Streptococcus, and Bifidobacterium and decreased quantities of Oscillospira, Bacteroides, and Parabacteroides compared to stools that were collected in small quantities and not mixed prior to DNA extraction. These findings indicate that certain taxa are at particular risk for under or over sampling due to protocol differences. Importantly, homogenization by any method significantly reduced the intra-individual variation in bacteria detected per stool. Our results confirm the robustness of fecal homogenization for microbial analyses and underscore the value of collecting and mixing large stool sample quantities in human nutrition intervention studies

Impact of sleeve gastrectomy compared to Roux-en-y gastric bypass upon hedonic hunger and the relationship to post-operative weight loss

'Hedonic hunger' indicates the desire to consume food in the absence of an energy requirement. Hedonic hunger can be investigated using the validated Power of Food Scale (PFS). Roux-en-Y gastric bypass (RYGB) and sleeve gastrectomy (SG) are currently the most effective treatment options for severe obesity. Following RYGB, hedonic hunger diminishes, which may contribute to sustained weight loss. There are no data examining the effect of SG on hedonic hunger. We prospectively evaluated hedonic hunger using PFS in patients with severe obesity prior to and 6 months after SG (n = 95) or RYGB (n = 44) and investigated the procedure-specific relationship between percentage weight loss (%WL) and hedonic hunger. Anthropometric data were collected at baseline after 6 months, 12 months and 24 months post-operatively. PFS contains 15 items grouped into 3 domains considering when food is: available (FA), present (FP), tasted (FT) and a total score (TS). At 6 months, a significant reduction was seen in all categories post-SG (p < 0.0001) and in TS (p = 0.003), FA (p = 0.0006) and FP (p = 0.0007) post-RYGB. A significantly larger reduction in FP scores was seen post-SG (p = 0.01). Post-SG, a significant correlation with 6-month %WL was noted for changes in FP (p = 0.03) and TS (p = 0.03). Post-SG changes in FP and TS predicted 24-month %WL. Post-RYGB significant correlations were seen between 6-month %WL and dFA (p = 0.04) and dFP (p = 0.03). Changes in FA, FP and TS were predictive of 12-month %WL. HH is reduced following both SG and RYGB with a greater reduction following SG and is related to post-operative %WL. PFS may have a role as a predictive tool for post-operative outcomes following SG and RYGB

Myopalladin promotes muscle growth through modulation of the serum response factor pathway

Myopalladin (MYPN) is a striated muscle-specific, immunoglobulin-containing protein located in the Z-line and I-band of the sarcomere as well as the nucleus. Heterozygous MYPN gene mutations are associated with hypertrophic, dilated, and restrictive cardiomyopathy, and homozygous loss-of-function truncating mutations have recently been identified in patients with cap myopathy, nemaline myopathy, and congenital myopathy with hanging big toe

Descriptive study of sociodemographic profile of population urban and rural at state of Minas Gerais

Este artigo teve por objetivo compreender como as variáveis relacionadas ao tipo de trabalho, à renda, ao acesso a bens de consumo, a serviços e tecnologias da informação e da comunicação manifestam-se no perfil das famílias que habitam o campo no Brasil. Utilizou-se dados da Pesquisa Nacional de Amostra de Domicílios (PNAD), referentes ao estado de Minas Gerais. Observou-se, em termos dos resultados finais, que o processo de mudança do modo de vida rural se dá de forma mais visível nas variáveis relacionadas ao consumo, no que se refere ao acesso à bens e serviços de origem urbana.This article aimed to understand how variables related to the type of work, income, access to goods, services and information and communication technologies are the profile of families inhabiting the field in Brazil. We used data from the National Household Sample Survey (PNAD), refering to the State of Minas Gerais. It was observed, in terms of final results, that the process of change of the rural way of life is most visible in the consumption-related variables, as regards access to goods and services of urban origin.Facultad de Humanidades y Ciencias de la Educació

Descriptive study of sociodemographic profile of population urban and rural at state of Minas Gerais

Este artigo teve por objetivo compreender como as variáveis relacionadas ao tipo de trabalho, à renda, ao acesso a bens de consumo, a serviços e tecnologias da informação e da comunicação manifestam-se no perfil das famílias que habitam o campo no Brasil. Utilizou-se dados da Pesquisa Nacional de Amostra de Domicílios (PNAD), referentes ao estado de Minas Gerais. Observou-se, em termos dos resultados finais, que o processo de mudança do modo de vida rural se dá de forma mais visível nas variáveis relacionadas ao consumo, no que se refere ao acesso à bens e serviços de origem urbana.This article aimed to understand how variables related to the type of work, income, access to goods, services and information and communication technologies are the profile of families inhabiting the field in Brazil. We used data from the National Household Sample Survey (PNAD), refering to the State of Minas Gerais. It was observed, in terms of final results, that the process of change of the rural way of life is most visible in the consumption-related variables, as regards access to goods and services of urban origin.Facultad de Humanidades y Ciencias de la Educació

Descriptive study of sociodemographic profile of population urban and rural at state of Minas Gerais

Este artigo teve por objetivo compreender como as variáveis relacionadas ao tipo de trabalho, à renda, ao acesso a bens de consumo, a serviços e tecnologias da informação e da comunicação manifestam-se no perfil das famílias que habitam o campo no Brasil. Utilizou-se dados da Pesquisa Nacional de Amostra de Domicílios (PNAD), referentes ao estado de Minas Gerais. Observou-se, em termos dos resultados finais, que o processo de mudança do modo de vida rural se dá de forma mais visível nas variáveis relacionadas ao consumo, no que se refere ao acesso à bens e serviços de origem urbana.This article aimed to understand how variables related to the type of work, income, access to goods, services and information and communication technologies are the profile of families inhabiting the field in Brazil. We used data from the National Household Sample Survey (PNAD), refering to the State of Minas Gerais. It was observed, in terms of final results, that the process of change of the rural way of life is most visible in the consumption-related variables, as regards access to goods and services of urban origin.Facultad de Humanidades y Ciencias de la Educació

The RADIOSTAR Project

© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).Radioactive nuclei are the key to understanding the circumstances of the birth of our Sun because meteoritic analysis has proven that many of them were present at that time. Their origin, however, has been so far elusive. The ERC-CoG-2016 RADIOSTAR project is dedicated to investigating the production of radioactive nuclei by nuclear reactions inside stars, their evolution in the Milky Way Galaxy, and their presence in molecular clouds. So far, we have discovered that: (i) radioactive nuclei produced by slow (107Pd and 182Hf) and rapid (129I and 247Cm) neutron captures originated from stellar sources —asymptotic giant branch (AGB) stars and compact binary mergers, respectively—within the galactic environment that predated the formation of the molecular cloud where the Sun was born; (ii) the time that elapsed from the birth of the cloud to the birth of the Sun was of the order of 107 years, and (iii) the abundances of the very short-lived nuclei 26Al, 36Cl, and 41Ca can be explained by massive star winds in single or binary systems, if these winds directly polluted the early Solar System. Our current and future work, as required to finalise the picture of the origin of radioactive nuclei in the Solar System, involves studying the possible origin of radioactive nuclei in the early Solar System from core-collapse supernovae, investigating the production of 107Pd in massive star winds, modelling the transport and mixing of radioactive nuclei in the galactic and molecular cloud medium, and calculating the galactic chemical evolution of 53Mn and 60Fe and of the p-process isotopes 92Nb and 146Sm.Peer reviewedFinal Published versio

Improving quality in nanoparticle-induced cytotoxicity testing by a tiered inter-laboratory comparison study

The quality and relevance of nanosafety studies constitute major challenges to ensure their key role as a supporting tool in sustainable innovation, and subsequent competitive economic advantage. However, the number of apparently contradictory and inconclusive research results has increased in the past few years, indicating the need to introduce harmonized protocols and good practices in the nanosafety research community. Therefore, we aimed to evaluate if best-practice training and inter-laboratory comparison (ILC) of performance of the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay for the cytotoxicity assessment of nanomaterials among 15 European laboratories can improve quality in nanosafety testing. We used two well-described model nanoparticles, 40-nm carboxylated polystyrene (PS-COOH) and 50-nm amino-modified polystyrene (PS-NH2). We followed a tiered approach using well-developed standard operating procedures (SOPs) and sharing the same cells, serum and nanoparticles. We started with determination of the cell growth rate (tier 1), followed by a method transfer phase, in which all laboratories performed the first ILC on the MTS assay (tier 2). Based on the outcome of tier 2 and a survey of laboratory practices, specific training was organized, and the MTS assay SOP was refined. This led to largely improved intra- and inter-laboratory reproducibility in tier 3. In addition, we confirmed that PS-COOH and PS-NH2 are suitable negative and positive control nanoparticles, respectively, to evaluate impact of nanomaterials on cell viability using the MTS assay. Overall, we have demonstrated that the tiered process followed here, with the use of SOPs and representative control nanomaterials, is necessary and makes it possible to achieve good inter-laboratory reproducibility, and therefore high-quality nanotoxicological data.Web of Science108art. no. 143

Genome-wide association study identifies 30 loci associated with bipolar disorder.

Bipolar disorder is a highly heritable psychiatric disorder. We performed a genome-wide association study (GWAS) including 20,352 cases and 31,358 controls of European descent, with follow-up analysis of 822 variants with P &lt; 1 × 10-4 in an additional 9,412 cases and 137,760 controls. Eight of the 19 variants that were genome-wide significant (P &lt; 5 × 10-8) in the discovery GWAS were not genome-wide significant in the combined analysis, consistent with small effect sizes and limited power but also with genetic heterogeneity. In the combined analysis, 30 loci were genome-wide significant, including 20 newly identified loci. The significant loci contain genes encoding ion channels, neurotransmitter transporters and synaptic components. Pathway analysis revealed nine significantly enriched gene sets, including regulation of insulin secretion and endocannabinoid signaling. Bipolar I disorder is strongly genetically correlated with schizophrenia, driven by psychosis, whereas bipolar II disorder is more strongly correlated with major depressive disorder. These findings address key clinical questions and provide potential biological mechanisms for bipolar disorder

The ocean sampling day consortium

Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of the marine microbial biodiversity and function of the world’s oceans. It is a simultaneous global mega-sequencing campaign aiming to generate the largest standardized microbial data set in a single day. This will be achievable only through the coordinated efforts of an Ocean Sampling Day Consortium, supportive partnerships and networks between sites. This commentary outlines the establishment, function and aims of the Consortium and describes our vision for a sustainable study of marine microbial communities and their embedded functional traits