Search for radiative pumping lines of OH masers: I. The 34.6um absorption line towards 1612 MHz OH maser sources

The 1612 MHz hydroxyl maser in circumstellar envelopes has long been thought to be pumped by 34.6um photons. Only recently, the Infrared Space Observatory has made possible spectroscopic observations which enable the direct confirmation of this pumping mechanism in a few cases. To look for the presence of this pumping line, we have searched the Infrared Space Observatory Data Archive and found 178 spectra with data around 34.6um for 87 galactic 1612MHz masers. The analysis performed showed that the noise level and the spectral resolution of the spectra are the most important factors affecting the detection of the 34.6um absorption line. Only 5 objects from the sample (3 red supergiants and 2 galactic center sources) are found to show clear 34.6um absorption (all of them already known) while two additional objects only tentatively show this line. The 3 supergiants show similar pump rates and their masers might be purely radiatively pumped. The pump rates of OH masers in late type stars are found to be about 0.05, only 1/5 of the theoretical value of 0.25 derived by Elitzur (1992). We have also found 16 maser sources which, according to the analysis assuming Elitzur's pump rate, should show the 34.6 $\mu$m absorption line but do not. These non-detections can be tentatively explained by far-infrared photon pumping, clumpy nature of the OH masing region or a limb-filling emission effect in the OH shell.Comment: 11 pages, 8 figures, 3 table

Genome-wide association and transcriptome studies identify target genes and risk loci for breast cancer

Genome-wide association studies (GWAS) have identified more than 170 breast cancer susceptibility loci. Here we hypothesize that some risk-associated variants might act in non-breast tissues, specifically adipose tissue and immune cells from blood and spleen. Using expression quantitative trait loci (eQTL) reported in these tissues, we identify 26 previously unreported, likely target genes of overall breast cancer risk variants, and 17 for estrogen receptor (ER)-negative breast cancer, several with a known immune function. We determine the directional effect of gene expression on disease risk measured based on single and multiple eQTL. In addition, using a gene-based test of association that considers eQTL from multiple tissues, we identify seven (and four) regions with variants associated with overall (and ER-negative) breast cancer risk, which were not reported in previous GWAS. Further investigation of the function of the implicated genes in breast and immune cells may provide insights into the etiology of breast cancer.Peer reviewe

The pyrimidin analogue cyclopentenyl cytosine induces alloantigen-specific non-responsiveness of human T lymphocytes

Cyclopentenyl cytosine (CPEC) has been shown to induce apoptosis in human T lymphoblastic cell lines and T cells from leukaemia patients. In this study we have addressed the question of whether CPEC is able to decrease proliferation and effector functions of human alloresponsive T lymphocytes and induce T cell anergy. The proliferative capacity of human peripheral blood mononuclear cells in response to allogeneic stimulation was measured by 5,6-carboxy-succinimidyl-diacetate-fluorescein-ester staining. Flow cytometric analysis was performed using surface CD4, CD8, CD25, CD103 and intracellular perforin, granzyme A, granzyme B, caspase-3 and forkhead box P3 (FoxP3) markers. The in vivo immunosuppressive capacity was tested in a murine skin graft model. Addition of CPEC at a concentration of 20 nM strongly decreased the expansion and cytotoxicity of alloreactive T cells. Specific restimulation in the absence of CPEC showed that the cells became anergic. The drug induced caspase-dependent apoptosis of alloreactive T lymphocytes. Finally, CPEC increased the percentage of CD25high FoxP3+ CD4+ and CD103+ CD8+ T cells, and potentiated the effect of rapamycin in increasing the numbers of alloreactive regulatory T cells. Treatment with CPEC of CBA/CA mice transplanted with B10/Br skin grafts significantly prolonged graft survival. We conclude that CPEC inhibits proliferation and cytotoxicity of human alloreactive T cells and induces alloantigen non-responsiveness in vitro