Virtual Round Table on Innovation for Smart and Sustainable Cities

A Dialogue between Paola Clerici Maestosi and Giovanni Vetritto (IT), Olga Kordas (SE), Johhanes Brezet (NL/DK) and Jonas Bylund (SE

Модель похибки вимірювального трансформатора напруги

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Phenol-Soluble Modulin α Peptide Toxins from Aggressive Staphylococcus aureus Induce Rapid Formation of Neutrophil Extracellular Traps through a Reactive Oxygen Species-Independent Pathway

Neutrophils have the ability to capture and kill microbes extracellularly through the formation of neutrophil extracellular traps (NETs). These are DNA and protein structures that neutrophils release extracellularly and are believed to function as a defense mechanism against microbes. The classic NET formation process, triggered by, e.g., bacteria, fungi, or by direct stimulation of protein kinase C through phorbol myristate acetate, is an active process that takes several hours and relies on the production of reactive oxygen species (ROS) that are further modified by myeloperoxidase (MPO). We show here that NET-like structures can also be formed by neutrophils after interaction with phenol-soluble modulin α (PSMα) that are cytotoxic membrane-disturbing peptides, secreted from community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA). The PSMα-induced NETs contained the typical protein markers and were able to capture microbes. The PSMα-induced NET structures were disintegrated upon prolonged exposure to DNase-positive S. aureus but not on exposure to DNase-negative Candida albicans. Opposed to classic NETosis, PSMα-triggered NET formation occurred very rapidly, independently of ROS or MPO, and was also manifest at 4°C. These data indicate that rapid NETs release may result from cytotoxic membrane disturbance by PSMα peptides, a process that may be of importance for CA-MRSA virulence

Triple access planning for uncertain futures - A handbook for practitioners

Planning for the future continues to evolve in the face of a changing world. What we did in the past will not work for the future. Even the recent shift, with greater interest in transport planning being vision-led and focused on people rather than traffic, it is still not enough.This Handbook is the next evolutionary step. It supports a way of thinking and acting that is intended to mark a change from transport planning in the ‘predict and provide’ paradigm to ‘Triple Access Planning’ in the ‘decide and provide’ paradigm. This is vision-led (‘decide’) instead of forecast-led (‘predict’). It includes digital accessibility alongside spatial proximity and mobility (together making ‘triple access’). It also includes addressing uncertainty about the future.The Handbook is a companion guide for those who are already conversant with transport planning or other planning approaches. It explains the triple access perspective on planning, the handling of uncertainty, addressing access for goods, and the organisational and institutional challenges associated with Triple Access Planning. For each of these themes, four planning phases are examined: (i) Philosophy - why take this approach?; (ii) Preparation and Analysis – assessing the current and future situations; (iii) Strategy Development – determining visions/goals and the approaches to achieving these; and (iv) Measure Planning – identifying more specifically what needs to be implemented to achieve goals. The first of these is an important (informal) orientation phase. The other phases reflect those recognised in European Sustainable Urban Mobility Planning (which also includes ‘Implementation and Monitoring’).Whilst Triple Access Planning does not offer an easier approach than established planning practices and institutions, it is more fit for purpose in relation to the challenge and opportunities we now face. This Handbook is a staging post in the onward journey of change in how we make sense of, and seek to be prepared for and shape, the future. Please embrace it

Activation of Neural and Pluripotent Stem Cell Signatures Correlates with Increased Malignancy in Human Glioma

The presence of stem cell characteristics in glioma cells raises the possibility that mechanisms promoting the maintenance and self-renewal of tissue specific stem cells have a similar function in tumor cells. Here we characterized human gliomas of various malignancy grades for the expression of stem cell regulatory proteins. We show that cells in high grade glioma co-express an array of markers defining neural stem cells (NSCs) and that these proteins can fulfill similar functions in tumor cells as in NSCs. However, in contrast to NSCs glioma cells co-express neural proteins together with pluripotent stem cell markers, including the transcription factors Oct4, Sox2, Nanog and Klf4. In line with this finding, in high grade gliomas mesodermal- and endodermal-specific transcription factors were detected together with neural proteins, a combination of lineage markers not normally present in the central nervous system. Persistent presence of pluripotent stem cell traits could only be detected in solid tumors, and observations based on in vitro studies and xenograft transplantations in mice imply that this presence is dependent on the combined activity of intrinsic and extrinsic regulatory cues. Together these results demonstrate a general deregulated expression of neural and pluripotent stem cell traits in malignant human gliomas, and indicate that stem cell regulatory factors may provide significant targets for therapeutic strategies

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements