The saguenay population register and the processing of occupational data: an overview of the methodology

Das Papier gibt einen Überblick über die Forschung, die in den letzten Jahren in der kanadischen Forschungsgruppe SOREP durchgeführt worden ist, um eine neue Methode zu entwickeln, die sich mit Berufsdaten befaßt. Diese Daten kommen von regionalen computerisierten Bevölkerungsregistern und sie wurden verschiedenen Verfahren unterzogen, um ihre Bedeutung und Nutzbarkeit herauszuarbeiten. Die Studie zeigt, daß soziale Abfragen, insbesondere hinsichtlich vergangener Mobilität zu dramatischen einseitigen Ergebnissen führen können, wenn nicht zuvor vorläufige kritische Arbeit erfolgt. Die Studie führt auch zu neuen Berufsklassifizierungsschemen, die hoffentlich einige der übrigen bekannten Defizite nicht aufweisen. Das Papier befaßt sich des weiteren mit den Problemen der Einschätzung des Inhalts von Berufsbezeichnungen als notwendigen Schritt zur korrekten Klassifizierung und Kategorisierung. Dabei wird ein grundsätzliches methodisches Problem aufgezeigt. Die Kriterien und Richtlinien, die benutzt werden sollten, um ein konsistentes und systematisches Klassifizierungsverfahren zu ermöglichen. Es wird gezeigt, daß der Gebrauch von technischen nicht notwendigerweise zu anachronistischen Aussagen führen muß, auch wenn vergangenheitsbezogene Berufsdaten klassifiziert werden sollen. (KW)'This paper outlines the research that has been carried out in the last few years within a Canadian research group (SOREP) to device a whole new methodology dealing with occupational data. These data come from a regional, computerized population register and they have been submitted to several processings in order to bring out their significance and possible uses. They study reveals that the social inquiries - namely into historical mobility - can be dramatically biased by a lack of preliminary, critical work. It also provides a new occupational classification grid which, hopefully, will avoid some of the usual, major known pitfalls. Finally, the paper adresses the problems of assessing the content of occupational titles, which is a necessary step allowing a proper classification into categories. Here, a basic methodological problem is pointed out: the criteria and guidelines that should be retained in order to achieve a consistent and systematic classifying process. It is shown that the use of contemporaneous technical scales, even to classify past occupational data, doesn't necessarily entails anachronism. (author's abstract

Alien Registration- Bouchard, Gerard H. (Saint John Plantation, Aroostook County)

https://digitalmaine.com/alien_docs/32487/thumbnail.jp

A genealogical study of essential hypertension with and without obesity in French Canadians

Objectives: To investigate genetic homogeneity in a set of hypertensive families and in subsets chosen for high and low prevalence of obesity; and to compare fasting insulin and lipids, ion transport, and water homeostasis in the obese and lean families. Research methods and procedures: The study was carried out in a relative population isolate of the Saguenay/Lac St. Jean region in Canada. Genetic homogeneity was evaluated with the mean coeffigcients of kinship (phi) and inbreeding (F) computed with ascending genealogies. Serum insulin and lipids were measured after overnight fasting. Total body water was estimated with bioelectrical impedance. Sodium-lithium countertransport and sodium-potassium co-transport were determined in freshly isolated erythrocytes. Results: F and phi were increased in hypertensive families compared with families selected at random. F and phi were further increased within the subsets of obese and lean families. In addition, fasting insulin, total body water, sodium-lithium countertransport, and sodium-potassium co-transport were higher in the obese than in the lean families. The two subsets of families did not differ by fasting lipids. Discussion: In the Saguenay/Lac St. Jean population, the degree of genetic homogeneity was increased in families selected for hypertension, and it was further increased in subsets of hypertensive families with high and low prevalence of obesity. This suggests that hypertension in lean and obese individuals may represent, at least in part, separate genetic entities. Some of the extra genes shared in common within the subsets may contribute to their differences in body weight, insulin sensitivity, ion transport, and water homeostasis

Leak-before-break: Global perspectives and procedures

Structural integrity of components containing fluids is critical for economic, environmental and safety issues. Any risk of catastrophic failure, in the form of either brittle or ductile manner, is not acceptable across the industries. Consequently, many efforts have been invested in the structural integrity aspect to improve the assessment methodologies. One of the ways to aid the decision whether or not to live with the defect is through the demonstration of Leak-Before-Break (LBB). LBB which is a well-established practice in the nuclear industry, albeit as a defence-in-depth argument or to justify the elimination of pipe whip restraints, also finds its applicability in other industries. A review of the available procedures, their associated limitations and the research carried out in the last thirty years is presented in this paper. Application of this concept within non-nuclear industries is also discussed

A Genome-Wide Association Study of Neuroticism in a Population-Based Sample

Neuroticism is a moderately heritable personality trait considered to be a risk factor for developing major depression, anxiety disorders and dementia. We performed a genome-wide association study in 2,235 participants drawn from a population-based study of neuroticism, making this the largest association study for neuroticism to date. Neuroticism was measured by the Eysenck Personality Questionnaire. After Quality Control, we analysed 430,000 autosomal SNPs together with an additional 1.2 million SNPs imputed with high quality from the Hap Map CEU samples. We found a very small effect of population stratification, corrected using one principal component, and some cryptic kinship that required no correction. NKAIN2 showed suggestive evidence of association with neuroticism as a main effect (p<10−6) and GPC6 showed suggestive evidence for interaction with age (p≈10−7). We found support for one previously-reported association (PDE4D), but failed to replicate other recent reports. These results suggest common SNP variation does not strongly influence neuroticism. Our study was powered to detect almost all SNPs explaining at least 2% of heritability, and so our results effectively exclude the existence of loci having a major effect on neuroticism

Epigenome-wide meta-analysis of blood DNA methylation in newborns and children identifies numerous loci related to gestational age

Background Preterm birth and shorter duration of pregnancy are associated with increased morbidity in neonatal and later life. As the epigenome is known to have an important role during fetal development, we investigated associations between gestational age and blood DNA methylation in children. Methods We performed meta-analysis of Illumina's HumanMethylation450-array associations between gestational age and cord blood DNA methylation in 3648 newborns from 17 cohorts without common pregnancy complications, induced delivery or caesarean section. We also explored associations of gestational age with DNA methylation measured at 4-18 years in additional pediatric cohorts. Follow-up analyses of DNA methylation and gene expression correlations were performed in cord blood. DNA methylation profiles were also explored in tissues relevant for gestational age health effects: fetal brain and lung. Results We identified 8899 CpGs in cord blood that were associated with gestational age (range 27-42 weeks), at Bonferroni significance, P <1.06 x 10(- 7), of which 3343 were novel. These were annotated to 4966 genes. After restricting findings to at least three significant adjacent CpGs, we identified 1276 CpGs annotated to 325 genes. Results were generally consistent when analyses were restricted to term births. Cord blood findings tended not to persist into childhood and adolescence. Pathway analyses identified enrichment for biological processes critical to embryonic development. Follow-up of identified genes showed correlations between gestational age and DNA methylation levels in fetal brain and lung tissue, as well as correlation with expression levels. Conclusions We identified numerous CpGs differentially methylated in relation to gestational age at birth that appear to reflect fetal developmental processes across tissues. These findings may contribute to understanding mechanisms linking gestational age to health effects.Peer reviewe

Meta-analysis of epigenome-wide association studies in neonates reveals widespread differential DNA methylation associated with birthweight

Birthweight is associated with health outcomes across the life course, DNA methylation may be an underlying mechanism. In this meta-analysis of epigenome-wide association studies of 8,825 neonates from 24 birth cohorts in the Pregnancy And Childhood Epigenetics Consortium, we find that DNA methylation in neonatal blood is associated with birthweight at 914 sites, with a difference in birthweight ranging from -183 to 178 grams per 10% increase in methylation (P-Bonferroni <1.06 x 10(-7)). In additional analyses in 7,278 participants,Peer reviewe

Genome-wide meta-analysis of 241,258 adults accounting for smoking behaviour identifies novel loci for obesity traits

Few genome-wide association studies (GWAS) account for environmental exposures, like smoking, potentially impacting the overall trait variance when investigating the genetic contribution to obesity-related traits. Here, we use GWAS data from 51,080 current smokers and 190,178 nonsmokers (87% European descent) to identify loci influencing BMI and central adiposity, measured as waist circumference and waist-to-hip ratio both adjusted for BMI. We identify 23 novel genetic loci, and 9 loci with convincing evidence of gene-smoking interaction (GxSMK) on obesity-related traits. We show consistent direction of effect for all identified loci and significance for 18 novel and for 5 interaction loci in an independent study sample. These loci highlight novel biological functions, including response to oxidative stress, addictive behaviour, and regulatory functions emphasizing the importance of accounting for environment in genetic analyses. Our results suggest that tobacco smoking may alter the genetic susceptibility to overall adiposity and body fat distribution.Peer reviewe

Deinococcus geothermalis: The Pool of Extreme Radiation Resistance Genes Shrinks

Bacteria of the genus Deinococcus are extremely resistant to ionizing radiation (IR), ultraviolet light (UV) and desiccation. The mesophile Deinococcus radiodurans was the first member of this group whose genome was completely sequenced. Analysis of the genome sequence of D. radiodurans, however, failed to identify unique DNA repair systems. To further delineate the genes underlying the resistance phenotypes, we report the whole-genome sequence of a second Deinococcus species, the thermophile Deinococcus geothermalis, which at its optimal growth temperature is as resistant to IR, UV and desiccation as D. radiodurans, and a comparative analysis of the two Deinococcus genomes. Many D. radiodurans genes previously implicated in resistance, but for which no sensitive phenotype was observed upon disruption, are absent in D. geothermalis. In contrast, most D. radiodurans genes whose mutants displayed a radiation-sensitive phenotype in D. radiodurans are conserved in D. geothermalis. Supporting the existence of a Deinococcus radiation response regulon, a common palindromic DNA motif was identified in a conserved set of genes associated with resistance, and a dedicated transcriptional regulator was predicted. We present the case that these two species evolved essentially the same diverse set of gene families, and that the extreme stress-resistance phenotypes of the Deinococcus lineage emerged progressively by amassing cell-cleaning systems from different sources, but not by acquisition of novel DNA repair systems. Our reconstruction of the genomic evolution of the Deinococcus-Thermus phylum indicates that the corresponding set of enzymes proliferated mainly in the common ancestor of Deinococcus. Results of the comparative analysis weaken the arguments for a role of higher-order chromosome alignment structures in resistance; more clearly define and substantially revise downward the number of uncharacterized genes that might participate in DNA repair and contribute to resistance; and strengthen the case for a role in survival of systems involved in manganese and iron homeostasis