Efeitos de um programa de condicionamento físico na funcionalidade de indivíduos com paralisia cerebral / Effects of a fitness program in functional capacity of individuals with cerebral palsy

Este estudo teve como objetivo analisar o ganho de funcionalidade em indivíduos com paralisia cerebral (PC) após um programa de condicionamento físico. Quatorze indivíduos diagnosticados com PC realizaram um programa de exercícios físicos que englobou exercícios aeróbicos, exercícios resistidos e alongamentos de membros inferiores realizados duas vezes por semana por um período de seis meses. Os participantes foram avaliados antes e após a intervenção através do teste de caminhada de seis minutos e de dez metros, índice de gasto energético (IGE), teste TimedUp and Go (TUG) e pelas dimensões D e E da Medida da Função Motora Grosa (GMFM). Todos os parâmetros analisados mostraram diferenças positivas após o programa de exercícios físicos e houve melhora estatisticamente significante com p0,05 no teste de caminhada de dez metros, teste de caminhada de seis minutos,TUG e no IGE. NoGMFM a dimensão D e E apresentaram diferenças,porém não foram estatisticamente significantes.Os achados neste estudo podem prover evidências que apoiam a efetividade de um programa de exercícios físicos em indivíduos com PC. Melhoras significativas no deslocamento e gasto energético foram observadas após seis meses de participação no programa de exercícios, o que sugere uma melhora na mobilidade funcional destes indivíduos.

Head control acquisition in preterm and fulhterm infants

A stable postural control is the basis for the organisation and voluntary execution of movements. In infants this control is shown by the development of motor abilities such as head control. Premature deliveries may generate risks to a child, such as lesions to the central nervous system, especially in the regions responsible for voluntary motricity. This study aimed at comparing the development of head control acquisition between preterm and full-term infants at the age of 2 and 4 months (preterm infant ages being corrected). Video recordings of five infants born prematurely and 5 born at term were analysed, within the Motor Development Follow-up Program For Preterm Born Children, maintained by the Department of Physical Therapy at the Londrina State University School Hospital. The items related to the development of head control included in the Gross Motor Function Measure (GMFM) were used in the evaluation. Although scores obtained by preterm and fullterm infants differed, the differences were found not to be statistically significant, thus suggesting that premature birth does not represent a risk for the final acquisition of head control. Despite the slightly late development in the acquisition of head control observed in preterm infants, both groups had similar scores at the age of four months.O controle postural estável constitui a base para a organização e execução voluntária do movimento. Esse controle exterioriza-se, noslactentes, pela emergência de habilidades motoras como o controle dacabeça. Partos prematuros podem gerar muitos riscos para a criança, como o comprometimento do sistema nervoso central, em especial de algumas regiões gerenciadoras da motricidade voluntária. O objetivo deste estudo foi comparar a evolução da aquisição do controle de cabeça entre lactentes nascidos pré-termo e a termo, aos 2 e 4 meses de idade, sendo a idade corrigida para os primeiros. Foram analisados videorregistros de cinco lactentes pré-termo e cinco a termo, incluídos em um Programa de Acompanhamento do Desenvolvimento Motor em Crianças Pré-termo do Departamento de Fisioterapia do Hospital Universitário do Norte do Paraná da UEL. Foram avaliados itens relacionados à aquisição do controle de cabeça do instrumento de avaliação G M F M (Gross Motor Function Measure). Os dados colhidos mostraram diferença de pontuação entre os grupos de lactentes pré-termo e a termo, mas a diferença não se mostrou estatisticamente significante, sugerindo que o nascimento pré-termo não afeta o controle de cabeça final. Apesar de um ligeiro atraso do desenvolvimento da aquisição do controle de cabeça dos lactentes prétermo, ao final dos 4 meses ambos os grupos apresentavam pontuaçõessimilares

Minimal information for studies of extracellular vesicles 2018 (MISEV2018):a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points

The James Webb Space Telescope Mission

Twenty-six years ago a small committee report, building on earlier studies, expounded a compelling and poetic vision for the future of astronomy, calling for an infrared-optimized space telescope with an aperture of at least $4m$. With the support of their governments in the US, Europe, and Canada, 20,000 people realized that vision as the $6.5m$ James Webb Space Telescope. A generation of astronomers will celebrate their accomplishments for the life of the mission, potentially as long as 20 years, and beyond. This report and the scientific discoveries that follow are extended thank-you notes to the 20,000 team members. The telescope is working perfectly, with much better image quality than expected. In this and accompanying papers, we give a brief history, describe the observatory, outline its objectives and current observing program, and discuss the inventions and people who made it possible. We cite detailed reports on the design and the measured performance on orbit.Comment: Accepted by PASP for the special issue on The James Webb Space Telescope Overview, 29 pages, 4 figure

Autologous haematopoietic stem cell transplantation for treatment of multiple sclerosis

Autologous haematopoietic stem cell transplantation (AHSCT) is a multistep procedure that enables destruction of the immune system and its reconstitution from haematopoietic stem cells. Originally developed for the treatment of haematological malignancies, the procedure has been adapted for the treatment of severe immune-mediated disorders. Results from ~20 years of research make a compelling case for selective use of AHSCT in patients with highly active multiple sclerosis (MS), and for controlled trials. Immunological studies support the notion that AHSCT causes qualitative immune resetting, and have provided insight into the mechanisms that might underlie the powerful treatment effects that last well beyond recovery of immune cell numbers. Indeed, studies have demonstrated that AHSCT can entirely suppress MS disease activity for 4–5 years in 70–80% of patients, a rate that is higher than those achieved with any other therapies for MS. Treatment-related mortality, which was 3.6% in studies before 2005, has decreased to 0.3% in studies since 2005. Current evidence indicates that the patients who are most likely to benefit from and tolerate AHSCT are young, ambulatory and have inflammatory MS activity. Clinical trials are required to rigorously test the efficacy, safety and cost-effectiveness of AHSCT against highly active MS drugs

Learning, retention and transfer of a new motor skill in children: comparison between mental and physical practice

O processo de aprendizagem motora permite, por meio de mudanças nos padrões de ativação de redes neurais, a consolidação de modelos internos dos movimentos. Atualmente sabe-se que esses modelos internos podem ser desenvolvidos não só pela prática física, mas também pela prática mental, definida como um ensaio mental repetido dos movimentos, sem a sua execução. Essa forma de prática permitiria a formação de um modelo interno mais flexível e independente do efetor. Embora a prática mental esteja sendo amplamente utilizada para o treino esportivo e a reabilitação em adultos, sua utilização em crianças ainda é muito restrita. Isto se deve, em parte, pela escassez de estudos que mostrem seus efeitos em crianças. Assim, o objetivo desse estudo foi comparar os efeitos da prática física e mental para a capacidade de aprender e reter uma nova habilidade motora de movimentos sequenciais de oposição de dedos, transferir essa aprendizagem para uma sequência reversa de movimentos e para a mão contralateral, não treinada, em crianças de 9 a 10 anos de idade. Participaram deste estudo 36 crianças destras com idade entre 9 e 10 anos divididas aleatoriamente em três grupos: grupo de prática mental (PM), composto por 12 crianças com média de idade de 9,9±0,3 anos; o grupo de prática física (PF), composto por 12 crianças com média de idade de 10±0,5 anos; e o grupo sem prática (SP), composto por 12 crianças de 9,9±0,4 anos. Os grupos de PM e PF foram submetidos a uma sessão de treino com 2400 repetições realizadas com a mão direita, sendo que o primeiro realizou a prática de forma imaginativa e o segundo a prática física. O grupo SP realizou uma atividade de pintura com duração similar ao treino dos demais grupos. O desempenho da sequência de movimentos que recebeu treinamento (ST), dos três grupos foi avaliado nas mesmas condições antes do treino (AT), e após 5 minutos (DT), 4 (4dDT), 7 (7dDT), 14 (14dDT), e 28 (28dDT), dias após o término do treinamento. O desempenho da sequência reversa a treinada (SR), realizada pela mão direita e o da ST na mão esquerda, não treinada (MNT), foi avaliado AT, DT, 7dDT e 28dDT. Os resultados obtidos por meio da ANOVA para medidas repetidas mostraram que não houve diferença entre os efeitos da PM e PF para a aprendizagem, retenção de curta e longa duração. Por outro lado, a ANOVA mostrou que, após o treino, a SR alcançou o mesmo desempenho da ST, bem como a MNT alcançou o mesmo desempenho da MT para a ST, apenas para o grupo que realizou a PM. Adicionalmente, conforme o esperado, o grupo SP não apresentou ganhos significativos de desempenho. Assim, baseados nesses resultados, pode-se concluir que crianças de 9 e 10 anos são capazes de aprender novas habilidades por meio da prática mental, que proporcionou os mesmos efeitos da prática física para a aprendizagem, retenção de curta e longa duração, e efeitos superiores para a transferência entre tarefas e intermanualMotor learning allows the consolidation of internal models of movement through activation patterns changes of neural networks. Currently it is known that these internal models can be developed not only by physical practice, but also by mental practice, defined as a mental rehearsal of movements repeated without the overt movement. Mental practice allows an internal model formation more flexible and independent of the effector. Although mental practice has been widely used for sports training and adults rehabilitation, pediatric use is still very limited. This is due, in part, to lack of studies which demonstrate effects in children. The objective of this study was to compare the effects of physical and mental practice on learning, retention of a new motor ability of sequential movements of fingers opposition and transfer this learning to a reverse sequence of movements and to the contralateral untrained hand, 9-10 years old children. This study included 36 right-handed children aged between 9 and 10 years old randomly allocated into three groups: mental practice (MP), composed of 12 children with a mean age of 9.9 ± 0.3 years, physical practice group (PP), composed of 12 children with mean age 10 ± 0.5 years, and the group without practice (NP), composed of 12 children aged 9.9 ± 0.4 years. The PP and MP groups were submitted to one session of training with 2400 repetitions with the dominant hand, and MP group trained using only mental rehearsal of movements PP group trained executing the movements. The NP group had a painting activity with similar duration of other groups. The performance of the sequence of movements that received training (TS) of the three groups was evaluated under the same conditions before training (BT), and after 5 minutes (AT), 4 (4dAT), 7 (7dAT), 14 (14dAT), and 28 (28dAT) days after training. The performance of the reverse sequence (RS) by the right trained hand (TH) and the performance of the trained sequence (TS) by the left untrained hand (UTH) was evaluated BT, AT, 7dAT and 28dAT. The results obtained by repeated measures ANOVA showed no differences between MP and PP for learning, retention of short and long term retention. Moreover, ANOVA revealed that, after training, only the MP group reached the same performance of the TS and RS, and UTH reached the same performance of the TS for the HT. Additionally, as expected, the NP group did not show significant performance gains. Thus, based on these results, it is possible to conclude that children aged 9 and 10 years old are able to learn new skills through mental practice, which provided the same effects of physical practice for learning, short and long term retention and higher effects for transfer between tasks and hand

Diabetes and the risk of non-Hodgkin's lymphoma and multiple myeloma in the European Prospective Investigation into Cancer and Nutrition.

BACKGROUND: Non-Hodgkin's lymphomas are a heterogeneous group of neoplasms arising from the lymphopoietic system including a wide range of subtypes of either B-cell or T-cell lymphomas. The few established risk factors for the development of these neoplasms include viral infections and immunological abnormalities, but their etiology remains largely unknown. Evidence suggests that certain medical conditions may be linked, through immunosuppression, to the risk of non-Hodgkin's lymphoma. Multiple myeloma is a neoplasm of plasma cells that accounts for approximately 15% of lymphopoietic cancers. Increases in the incidence of non-Hodgkin's lymphoma and multiple myeloma in the past implicate environmental factors as potential causal agents. DESIGN AND METHODS: In the European Prospective Investigation into Cancer and Nutrition (EPIC), 1,213 histologically confirmed incident cases of non-Hodgkin's lymphoma and multiple myeloma (594 men; 619 women) were identified during a follow-up of 8.5 years. Cox proportional hazard models were used to explore the association between self-reported diabetes, diagnosed after 30 years of age, and the risk of non-Hodgkin's lymphoma overall and multiple myeloma and various lymphoma subtypes. RESULTS: We found no association between a personal history of diabetes and the risk of non-Hodgkin's lymphoma overall in men (HR: 1.28, 95% CI: 0.89-1.84), in women (HR: 0.71, 95% CI: 0.41- 1.24), or in men and women combined (HR: 1.09, 95% CI: 0.80-1.47). Among the B-non-Hodgkin's lymphoma subtypes, we observed a statistically significant increased risk of B-cell chronic lymphocytic leukemia (HR: 2.0, 95% CI: 1.04-3.86) in men, but not in women (HR: 1.07, 95% CI: 0.33-3.43). CONCLUSIONS: This prospective study did not provide evidence for a role of self-reported diabetes in the etiology of non-Hodgkin's lymphoma overall or multiple myeloma. We found an increased risk of B-cell chronic lymphocytic leukemia among men with diabetes, but not among women. We hypothesize that diabetes may not play a causal role in the etiology of B-cell chronic lymphocytic leukemia, though the underlying pathogenic mechanisms of both disorders may include shared genetic, host and/or environmental susceptibility factors