Genome-wide analysis of cell wall-related genes in Tuber melanosporum

A genome-wide inventory of proteins involved in cell wall synthesis and remodeling has been obtained by taking advantage of the recently released genome sequence of the ectomycorrhizal black truffle. Genes that encode cell wall biosynthetic enzymes, enzymes involved in cell wall polysaccharide synthesis or modification, GPI-anchored proteins and other cell wall proteins were identified in the black truffle genome. As a second step, array data were validated and the symbiotic stage was chosen as the main focus. Quantitative RT-PCR experiments were performed on 29 selected genes to verify their expression during ectomycorrhizal formation. The results confirmed the array data, and this suggests that cell wall-related genes are required for morphogenetic transition from mycelium growth to the ectomycorrhizal branched hyphae. Labeling experiments were also performed on mycelium and ectomycorrhizae to localize cell wall components

Functional aspects of root architecture and mycorrhizal inoculation with respect to nutrient uptake capacity

ACESSO via B-on: http://dx.doi.org/10.1007/s00572-003-0254-5The aim of this research was to investigate theeffect of arbuscular mycorrhizal (AM) colonisation onroot morphology and nitrogen uptake capacity of carob(Ceratonia siliqua L.) under high and low nutrientconditions. The experimental design was a factorialarrangement of presence/absence of mycorrhizal fungusinoculation (Glomus intraradices) and high/low nutrientstatus. Percent AM colonisation, nitrate and ammoniumuptake capacity, and nitrogen and phosphorus contentswere determined in 3-month-old seedlings. Grayscale andcolour images were used to study root morphology andtopology, and to assess the relation between rootpigmentation and physiological activities. AM colonisationlead to a higher allocation of biomass to white andyellow parts of the root. Inorganic nitrogen uptakecapacity per unit root length and nitrogen content weregreatest in AM colonised plants grown under low nutrientconditions. A better match was found between plantnitrogen content and biomass accumulation, than betweenplant phosphorus content and biomass accumulation. It issuggested that the increase in nutrient uptake capacity ofAM colonised roots is dependent both on changes in rootmorphology and physiological uptake potential. Thisstudy contributes to an understanding of the role of AMfungi and root morphology in plant nutrient uptake andshows that AM colonisation improves the nitrogennutrition of plants, mainly when growing at low levelsof nutrients

Highlights of the mini-symposium on extracellular vesicles in inter-organismal communication, held in Munich, Germany, August 2018

All living organisms secrete molecules for intercellular communication. Recent research has revealed that extracellular vesicles (EVs) play an important role in inter-organismal cell-to-cell communication by transporting diverse messenger molecules, including RNA, DNA, lipids and proteins. These discoveries have raised fundamental questions regarding EV biology. How are EVs biosynthesized and loaded with messenger/cargo molecules? How are EVs secreted into the extracellular matrix? What are the EV uptake mechanisms of recipient cells? As EVs are produced by all kind of organisms, from unicellular bacteria and protists, filamentous fungi and oomycetes, to complex multicellular life forms such as plants and animals, basic research in diverse model systems is urgently needed to shed light on the multifaceted biology of EVs and their role in inter-organismal communications. To help catalyse progress in this emerging field, a mini-symposium was held in Munich, Germany in August 2018. This report highlights recent progress and major questions being pursued across a very diverse group of model systems, all united by the question of how EVs contribute to inter-organismal communication

Tectonic evolution of the Eastern Moroccan Meseta: from Late Devonian fore‐arc sedimentation to Early Carboniferous collision of an Avalonian promontory

This study was founded by the Ministerio de Economia y Competitividad (MINECO) of Spain through the project PANGEATOR (CGL2015-71692-P) and the Doctoral scholarship BES-2016-078168. GeoHistory Facility instruments were funded via an Australian Geophysical Observing System grant provided to AuScope Pty Ltd. by the AQ44 Australian Education Investment Fund program. The NPII multicollector was obtained via funding from the Australian Research Council LIEF program (LE150100013). The authors want to express their gratitude to Dr. Manuel Francisco Pereira (University of Evora, Portugal) and Dr. Michel Villeneuve (Centre Europeen de Recherche et d'Enseignement des Geosciences de l'Environnement, France) for their constructive reviews that helped to improve the quality of the original manuscript. Special thanks to Brad McDonald (Curtin University, Australia) for technical assistance regarding LA-ICPMS and Hf analyses, Profs. Abdelfatah Tahiri (University Mohammed V of Rabat, Morocco) and Hassan El Hadi (University Hassan II of Casablanca, Morocco) for their support during field work, Prof. Yvette Kuiper (Colorado School of Mines, USA) for her precious hints about the interpretation of Hf data, and Dr. Lorenzo Valetti for proofreading the manuscript. Supporting information can be obtained in Mendeley Data: https://doi.org/10.17632/b8fdbykmbx.1 (https://data.mendeley.com/datasets/b8fdbykmbx/draft?a=eaae2da0-8e224056-861b-4824984f1c10).The deformed Paleozoic succession of the Eastern Moroccan Meseta crops out in relativelysmall and isolated inliers surrounded by Mesozoic and Cenozoic rocks. Two of the largest inliers(Mekkam and Debdou) are characterized by a monotonous succession of slates and greywackes affected bypolyphasic folding that occurred at low‐to very low grade metamorphic conditions. New U‐Pb ages ondetrital zircon grains from the Debdou‐Mekkam metasediments constrain the maximal depositional age asLate Devonian, interpreted to be close to the true sedimentation age. Furthermore, theεHfvalues of theDevonian detrital zircons, together with the presence of a series of scattered zircon grains with ages betweenc. 0.9 and c. 1.9 Ga, suggest provenance from a subduction‐related magmatic arc located on the Avalonianmargin. The Debdou‐Mekkam massif is characterized by an Early Carboniferousfirst deformationalevent (D1), which gave way to a pervasive cleavage (S1) associated with plurikilometric‐scale, tight toisoclinal, overturned to recumbent folds. Later events (Dc) occurred at Late Carboniferous time andgenerated variably developed crenulation cleavages (Sc) associated with variously oriented metric‐tokilometric‐scale folds, which complicate the pattern of both D1 intersection lineations (L1) and axial traces.The restoration of this pronounced curved pattern yields originally SW‐NE‐oriented D1 fold axes withregional SE‐vergence. This important Early Carboniferous shortening and SE‐directed tectonic transport canbe explained by closure of the Rheic Ocean and thefirst phases of the collision between the northern passivemargin of Gondwana and an Avalonian promontory.Ministerio de Economia y Competitividad (MINECO) of Spain CGL2015-71692-P BES-2016-078168Australian Geophysical Observing SystemAustralian Education Investment Fund program AQ44Australian Research Council LE15010001

Development of an analogue optical link for the front-end read-out of the ATLAS electromagnetic calorimeter

We have developed an analogue optical data transmission system intended to meet the read-out requirements of the ATLAS liquid argon electromagnetic calorimeter. Eight-way demonstrators have been built and tested. The link uses arrays of VCSEL diodes as the optical emitters, coupled to a 70 m long fibre ribbon to simulate the distance between the detector and the control room. The receiver is based around a custom-designed PIN photodiode array. We describe here the final results of laboratory tests on a demonstrator, laying stress on the VCSEL-to-fibre coupling issues, and the overall performance of the full link. A 9-bit dynamic range is achieved, with a 5on-linearity

Future changes in consumption: The income effect on greenhouse gas emissions

The scale and patterns of household consumption are important determinants of environmental impacts. Whilst affluence has been shown to have a strong correlation with environmental impact, they do not necessarily grow at the same rate. Given the apparent contradiction between the sustainable development goals of economic growth and environmental protection, it is important to understand the effect of rising affluence and concurrent changing consumption patterns on future environmental impacts. Here we develop an econometric demand model based on the data available from a global multiregional input-output dataset. We model future household consumption following scenarios of population and GDP growth for 49 individual regions. The greenhouse gas (GHG) emissions resulting from the future household demand is then explored both with and without consideration of the change in expenditure over time on different consumption categories. Compared to a baseline scenario where final demand grows in line with the 2011 average consumption pattern up until 2030, we find that changing consumer preferences with increasing affluence has a small negative effect on global cumulative GHG emissions. The differences are more profound on both a regional and a product level. For the demand model scenario, we find the largest decrease in GHG emissions for the BRICS and other developing countries, while emissions in North America and the EU remain unchanged. Decreased spending and resulting emissions on food are cancelled out by increased spending and emissions on transportation. Despite relatively small global differences between the scenarios, the regional and sectoral wedges indicate that there is a large untapped potential in environmental policies and lifestyle changes that can complement the technological transition towards a low-emitting society

Deciphering exome sequencing data: Bringing mitochondrial DNA variants to light

The expanding use of exome sequencing (ES) in diagnosis generates a huge amount of data, including untargeted mitochondrial DNA (mtDNA) sequences. We developed a strategy to deeply study ES data, focusing on the mtDNA genome on a large unspecific cohort to increase diagnostic yield. A targeted bioinformatics pipeline assembled mitochondrial genome from ES data to detect pathogenic mtDNA variants in parallel with the "in-house" nuclear exome pipeline. mtDNA data coming from off-target sequences (indirect sequencing) were extracted from the BAM files in 928 individuals with developmental and/or neurological anomalies. The mtDNA variants were filtered out based on database information, cohort frequencies, haplogroups and protein consequences. Two homoplasmic pathogenic variants (m.9035T>C and m.11778G>A) were identified in 2 out of 928 unrelated individuals (0.2%): the m.9035T>C (MT-ATP6) variant in a female with ataxia and the m.11778G>A (MT-ND4) variant in a male with a complex mosaic disorder and a severe ophthalmological phenotype, uncovering undiagnosed Leber\u27s hereditary optic neuropathy (LHON). Seven secondary findings were also found, predisposing to deafness or LHON, in 7 out of 928 individuals (0.75%). This study demonstrates the usefulness of including a targeted strategy in ES pipeline to detect mtDNA variants, improving results in diagnosis and research, without resampling patients and performing targeted mtDNA strategies

Position resolution and particle identification with the ATLAS EM calorimeter

In the years between 2000 and 2002 several pre-series and series modules of the ATLAS EM barrel and end-cap calorimeter were exposed to electron, photon and pion beams. The performance of the calorimeter with respect to its finely segmented first sampling has been studied. The polar angle resolution has been found to be in the range 50-60 mrad/sqrt(E (GeV)). The neutral pion rejection has been measured to be about 3.5 for 90% photon selection efficiency at pT=50 GeV/c. Electron-pion separation studies have indicated that a pion fake rate of (0.07-0.5)% can be achieved while maintaining 90% electron identification efficiency for energies up to 40 GeV.Comment: 32 pages, 22 figures, to be published in NIM

Measurement of the cross-section and charge asymmetry of WW bosons produced in proton-proton collisions at s=8\sqrt{s}=8 TeV with the ATLAS detector

This paper presents measurements of the $W^+ \rightarrow \mu^+\nu$ and $W^- \rightarrow \mu^-\nu$ cross-sections and the associated charge asymmetry as a function of the absolute pseudorapidity of the decay muon. The data were collected in proton--proton collisions at a centre-of-mass energy of 8 TeV with the ATLAS experiment at the LHC and correspond to a total integrated luminosity of 20.2~\mbox{fb^{-1}}. The precision of the cross-section measurements varies between 0.8% to 1.5% as a function of the pseudorapidity, excluding the 1.9% uncertainty on the integrated luminosity. The charge asymmetry is measured with an uncertainty between 0.002 and 0.003. The results are compared with predictions based on next-to-next-to-leading-order calculations with various parton distribution functions and have the sensitivity to discriminate between them.Comment: 38 pages in total, author list starting page 22, 5 figures, 4 tables, submitted to EPJC. All figures including auxiliary figures are available at https://atlas.web.cern.ch/Atlas/GROUPS/PHYSICS/PAPERS/STDM-2017-13

Search for chargino-neutralino production with mass splittings near the electroweak scale in three-lepton final states in √s=13 TeV pp collisions with the ATLAS detector

A search for supersymmetry through the pair production of electroweakinos with mass splittings near the electroweak scale and decaying via on-shell W and Z bosons is presented for a three-lepton final state. The analyzed proton-proton collision data taken at a center-of-mass energy of √s=13  TeV were collected between 2015 and 2018 by the ATLAS experiment at the Large Hadron Collider, corresponding to an integrated luminosity of 139  fb−1. A search, emulating the recursive jigsaw reconstruction technique with easily reproducible laboratory-frame variables, is performed. The two excesses observed in the 2015–2016 data recursive jigsaw analysis in the low-mass three-lepton phase space are reproduced. Results with the full data set are in agreement with the Standard Model expectations. They are interpreted to set exclusion limits at the 95% confidence level on simplified models of chargino-neutralino pair production for masses up to 345 GeV