A multi-phase linear kinematic elastoplastic model for the HAZ of welded S355J2 steel under low-cycle fatigue

International audienc

Advancing the Contour Method for the Measurement of Residual Stress in Unbalanced Asymmetric CFRP Laminate

Residual stresses are inevitably generated in composites during the curing phase of manufacture, where cooling from high cure temperature to room temperature is accompanied by constrained shrinkage. The high cure temperature represents a stress free state owing to the viscous nature of the matrix. Due to their inherent anisotropic and heterogeneous nature, both fibre and matrix constituents contract and expand differently leading to constraint induced differences in strains between fibre and matrix at the micro-scale and between plies having different fibre orientations at the macro-scale. The resulting residual stresses are directly associated with dimensional stability such as warpage, shape distortion and the structural integrity of composite structures (e.g matrix cracking, reduced fibre-matrix bondage and delamination). Therefore, knowledge and accurate characterisation of residual stress is imperative for optimising the design and structural integrity of polymer composites. Up until now, numerous analytical, computational and experimental methods have been developed to characterise residual stresses in polymer composites. However for analytical models, knowledge of anisotropic viscoelastic behaviour of materials involves extensive characterisation in order to capture complex thermo-chemo-rheological properties [1]. Numerical models get computationally expensive when internal state variables such as cure and temperature dependent viscoelasticity, mechanical properties and chemical shrinkage of resin are considered to simulate the entire complicated curing cycle. Furthermore, composites may also undergo temperature and moisture changes during and after the manufacturing process inducing hygrothermal residual stress; but these parameters cannot be readily predicted in numerical or analytical models. In addition, the reliability of the numerical and analytical solutions are highly dependent on the quality of input data. Experimental measurements offer an alternative solution for developing a quantitative understanding of the sign, magnitude and distribution of residual stresses. But there is no consensus regarding what experimental method to use for measuring bulk (through-thickness) residual stress in polymer composites. The present PhD research will investigate the viability of using the Contour Method for measuring residual stress in polymer composites. The contour method is a powerful measurement technique that provides two-dimensional (2D) map of residual stress in engineering components [2]. It involves sectioning the component in two halves along the plane of interest, measuring the deformation of the created cut surfaces and use the measured deformations to back calculate residual stresses by an elastic stress analysis. Traditionally, the contour method has been solely applied to metallic structures. The challenge to address in this research is to expand its applications to non-metallic materials such as composites

Experimental characterization and numerical modeling of the influence of a proof load on the fatigue resistance of welded structures

The influence of a proof load, or an initial overload, on the fatigue resistance of welded structures is investigated in this study. Arc welded stiffeners made of S355 steel are considered and fatigue-tested in as-welded and proof-loaded conditions. X-ray diffraction analyses are carried out to evaluate the influence of a proof load on the residual stress field. To better understand the role of a proof load on the mechanical fields in the stress concentration zones, a numerical approach based on elasto-plastic finite element analyses is proposed. The methodology includes a non-local multiaxial fatigue criterion, which integrates a strain hardening contribution, that makes it possible to estimate the number of cycles to failure. The comparison between experimental and numerical results indicates that the beneficial effect of a proof load on the fatigue resistance can be mainly attributed to residual stress and strain hardening effects. The final result is a master SN curve adapted to the fatigue design of stiffener structures, tested under different loading conditions

Lessons from prospective longitudinal follow-up of a French APECED cohort

Background Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy syndrome is a rare disease caused by biallelic mutations of the AIRE gene, usually presenting with the triad hypoparathyroidism-adrenal failure-chronic mucocutaneous candidiasis (CMC) and nonendocrine manifestations. The aim of this study was to determine the molecular profile of the AIRE gene, the prevalence of rare manifestations, and to characterize immunological disturbances in a French cohort. Patients and Methods A national, multicenter prospective observational study to collect genetic, clinical, biological, and immunological data (NCT03751683). Results Twenty-five patients (23 families) were enrolled. Eleven distinct AIRE variants were identified, 2 of which were not previously reported: an intronic variant, c.653-70G > A, and a c.1066del (p.Arg356GlyfsX22) variant (exon 9). The most common was the Finnish variant c.769C > T (16 alleles), followed by the variant c.967_979del13 (15 alleles), which seemed associated with a less severe phenotype. Seventeen out of 25 patients were homozygote. The median number of clinical manifestations was 7; 19/25 patients presented with the hypoparathyroidism-adrenal failure-CMC triad, 8/13 showed pulmonary involvement, 20/25 had ectodermal dystrophy, 8/25 had malabsorption, and 6/23 had asplenia. Fifteen out of 19 patients had natural killer cell lymphopenia with an increase in CD4+ and CD8+ T lymphocytes and an age-dependent alteration of B lymphocyte homeostasis compared with matched controls (P < .001), related to the severity of the disease. All tested sera (n = 18) were positive for anti-interferon-α, 15/18 for anti-IL-22 antibodies, and 13/18 for anti-IL-17F antibodies, without clear phenotypic correlation other than with CMC. Conclusion This first prospective cohort showed a high AIRE genotype variability, with 2 new gene variants. The prevalence of potentially life-threatening nonendocrine manifestations was higher with systematic screening. These manifestations could, along with age-dependent B-cell lymphopenia, contribute to disease severity. Systematic screening for all the manifestations of the syndrome would allow earlier diagnosis, supporting vaccination and targeted therapeutic approaches

Hemidesmosome integrity protects the colon against colitis and colorectal cancer

OBJECTIVE: Epidemiological and clinical data indicate that patients suffering from IBD with long-standing colitis display a higher risk to develop colorectal high-grade dysplasia. Whereas carcinoma invasion and metastasis rely on basement membrane (BM) disruption, experimental evidence is lacking regarding the potential contribution of epithelial cell/BM anchorage on inflammation onset and subsequent neoplastic transformation of inflammatory lesions. Herein, we analyse the role of the alpha6beta4 integrin receptor found in hemidesmosomes that attach intestinal epithelial cells (IECs) to the laminin-containing BM. DESIGN: We developed new mouse models inducing IEC-specific ablation of alpha6 integrin either during development (alpha6DeltaIEC) or in adults (alpha6DeltaIEC-TAM). RESULTS: Strikingly, all alpha6DeltaIEC mutant mice spontaneously developed long-standing colitis, which degenerated overtime into infiltrating adenocarcinoma. The sequence of events leading to disease onset entails hemidesmosome disruption, BM detachment, IL-18 overproduction by IECs, hyperplasia and enhanced intestinal permeability. Likewise, IEC-specific ablation of alpha6 integrin induced in adult mice (alpha6DeltaIEC-TAM) resulted in fully penetrant colitis and tumour progression. Whereas broad-spectrum antibiotic treatment lowered tissue pathology and IL-1beta secretion from infiltrating myeloid cells, it failed to reduce Th1 and Th17 response. Interestingly, while the initial intestinal inflammation occurred independently of the adaptive immune system, tumourigenesis required B and T lymphocyte activation. CONCLUSIONS: We provide for the first time evidence that loss of IECs/BM interactions triggered by hemidesmosome disruption initiates the development of inflammatory lesions that progress into high-grade dysplasia and carcinoma. Colorectal neoplasia in our mouse models resemble that seen in patients with IBD, making them highly attractive for discovering more efficient therapies.PMC559510

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements

26th Annual Computational Neuroscience Meeting (CNS*2017): Part 3 - Meeting Abstracts - Antwerp, Belgium. 15–20 July 2017

This work was produced as part of the activities of FAPESP Research,\ud Disseminations and Innovation Center for Neuromathematics (grant\ud 2013/07699-0, S. Paulo Research Foundation). NLK is supported by a\ud FAPESP postdoctoral fellowship (grant 2016/03855-5). ACR is partially\ud supported by a CNPq fellowship (grant 306251/2014-0)