I Want A Dixie Sweetheart

https://digitalcommons.library.umaine.edu/mmb-vp/1699/thumbnail.jp

Modeling Optical Properties of Liquid-Crystal Devices by Numerical Solution of Time-Harmonic Maxwell Equations

this paper, we discuss a method for solving a formulation in the frequency domain, i.e., for the time-harmonic form of the Maxwell equations. Such techniques have been used by numerical analysts in other setting

I want a Dixie sweetheart

(Published By C.C. Church

Metabolism of D-aminoacyl-tRNAs in Escherichia coli and Saccharomyces cerevisiae cells.

International audienceIn Escherichia coli, tyrosyl-tRNA synthetase is known to esterify tRNA(Tyr) with tyrosine. Resulting d-Tyr-tRNA(Tyr) can be hydrolyzed by a d-Tyr-tRNA(Tyr) deacylase. By monitoring E. coli growth in liquid medium, we systematically searched for other d-amino acids, the toxicity of which might be exacerbated by the inactivation of the gene encoding d-Tyr-tRNA(Tyr) deacylase. In addition to the already documented case of d-tyrosine, positive responses were obtained with d-tryptophan, d-aspartate, d-serine, and d-glutamine. In agreement with this observation, production of d-Asp-tRNA(Asp) and d-Trp-tRNA(Trp) by aspartyl-tRNA synthetase and tryptophanyl-tRNA synthetase, respectively, was established in vitro. Furthermore, the two d-aminoacylated tRNAs behaved as substrates of purified E. coli d-Tyr-tRNA(Tyr) deacylase. These results indicate that an unexpected high number of d-amino acids can impair the bacterium growth through the accumulation of d-aminoacyl-tRNA molecules and that d-Tyr-tRNA(Tyr) deacylase has a specificity broad enough to recycle any of these molecules. The same strategy of screening was applied using Saccharomyces cerevisiae, the tyrosyl-tRNA synthetase of which also produces d-Tyr-tRNA(Tyr), and which, like E. coli, possesses a d-Tyr-tRNA(Tyr) deacylase activity. In this case, inhibition of growth by the various 19 d-amino acids was followed on solid medium. Two isogenic strains containing or not the deacylase were compared. Toxic effects of d-tyrosine and d-leucine were reinforced upon deprivation of the deacylase. This observation suggests that, in yeast, at least two d-amino acids succeed in being transferred onto tRNAs and that, like in E. coli, the resulting two d-aminoacyl-tRNAs are substrates of a same d-aminoacyl-tRNA deacylase

How early childhood events impact upon adults health

Early childhood events (ECEs) impact on biological, psychological and social development to influence health behaviour and outcomes in later life. The research evidence clearly shows that adverse childhood experiences are associated with increased health risk behaviours, increased morbidity and early mortality. The evidence also shows that adversity affects different people in different ways, and not all children exposed to adversity will be negatively impacted. Positive events in early childhood, such as supportive relationships, can strengthen biological, psychological and social development and can protect against some of the harmful effects of childhood adversity. Childhood adversity, which is relatively common in the general population, has been shown to disproportionally affect those living in lower socioeconomic conditions. Preventing childhood adversity should be considered within the broader context of addressing societal inequalities. Interventions should focus on supporting parenting, building relationships and resilience, early identification of adversity and trauma-focused care