163 research outputs found
Oxidative Stress And Changes In The Content And Pattern Of Tissue Expression Of β-catenin Protein In Diversion Colitis
Objective: The aim of this study is to verify if oxidative stress is related to changes in content and pattern of β-catenin protein expression in an experimental model of diversion colitis. Methods: Sixty Wistar rats were submitted to intestinal bypass. The animals were divided into three groups according to the sacrifice to take place in six, 12 and 18 weeks. For each group, five animals only underwent laparotomy (control). The presence of colitis was diagnosed by histological study, and its severity, by inflammation grading scale. Cellular oxidative stress was measured by comet assay. Tissue expression of β-catenin protein was analyzed by the immunohistochemistry and quantification of its tissue content by computerized morphometry. Statistical analysis was performed with the Student's t-test, median, Mann-Whitney, ANOVA and Kruskal-Wallis, adopting a significance level of 5% (p <0.05). Results: Colon segments without fecal stream developed colitis, which worsened with time of exclusion. Segments without fecal stream suffer higher levels of oxidative stress when compared to those with stream, and it worsens with time of exclusion. The levels of cellular oxidative stress are directly related to the degree of inflammation. The total content of β-catenin in segments without fecal stream reduces after six weeks, and does not vary thereafter. The content of β-catenin in the apical portion of the colon crypts decreases with time, whereas in the basal region, it increases. The total content of β-catenin is inversely related to the degree of inflammation and levels of tissue oxidative stress levels. Conclusion: There are changes in tissue content of E-cadherin and increased expression of β-catenin in proliferative regions of colonic crypts, related with oxidative tissue stress.324343358Pravda, J., Radical induction theory of ulcerative colitis (2005) World J Gastroenterol, 11 (16), pp. 2371-2384Gaudier, E., Hoebler, C., Physiological role of mucins in the colonic barrier integrity (2006) Gastroenterol Clin Biol, 30 (8-9), pp. 965-974Laukoetter, M.G., Nava, P., Nusrat, A., Role of the intestinal barrier in inflammatory bowel disease (2008) World J Gastroenterol, 14 (3), pp. 401-407Berkes, J., Viswananthan, V.K., Savkovic, S.D., Hecht, G., Intestinal epithelial responses to enteric pathogens: Effects on the tight junction barrier, iron transport, and inflammation (2003) Gut, 52 (3), pp. 439-451Clayburgh, D.R., Shen, L., Turner, J.R., A porous defense: The leaky epithelial barrier in intestinal disease (2004) Lab Invest, 84 (3), pp. 282-291Usami, Y., Chiba, H., Nakayama, F., Ueda, J., Matsuda, Y., Sawada, N., Reduced expression of claudin-7 correlates with invasion and metastasis in squamous cell carcinoma of the esophagus (2006) Hum Pathol, 37 (5), pp. 569-577Gumbiner, B., Stevenson, B., Grimaldi, A., The role of the cell adhesion molecule uvomorulin in the formation and maintenance of the epithelial junctional complex (1988) J Cell Biol, 107 (4), pp. 1575-1587Gumbiner, B.M., McCrea, P.D., Catenins as mediators of the cytoplasmic functions of cadherins (1993) J Cell Sci Suppl, 17, pp. 155-158Yeager, M., Unger, V.M., Falk, M.M., Synthesis, assembly and structure of gap junction intercellular channels (1998) Curr Opin Struct Biol, 8 (6), pp. 810-811Hynes, R.O., Zhao, Q., The evolution of cell adhesion (2000) J Cell Biol, 150 (2), pp. F89-F96Kypta, R., Bernfield, M., Burridge, K., Geiger, B., Goodenough, D., Humphries, M., Hynes, R., Yurchenco, P., Junções celulares, adesão celular e matriz extracelular (2006) Biologia Molecular Da Célula, pp. 1065-1125. , In: Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P. 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Centrality dependence of charged particle production at large transverse momentum in Pb-Pb collisions at TeV
The inclusive transverse momentum () distributions of primary
charged particles are measured in the pseudo-rapidity range as a
function of event centrality in Pb-Pb collisions at
TeV with ALICE at the LHC. The data are presented in the range
GeV/ for nine centrality intervals from 70-80% to 0-5%.
The Pb-Pb spectra are presented in terms of the nuclear modification factor
using a pp reference spectrum measured at the same collision
energy. We observe that the suppression of high- particles strongly
depends on event centrality. In central collisions (0-5%) the yield is most
suppressed with at -7 GeV/. Above
GeV/, there is a significant rise in the nuclear modification
factor, which reaches for GeV/. In
peripheral collisions (70-80%), the suppression is weaker with almost independently of . The measured nuclear
modification factors are compared to other measurements and model calculations.Comment: 17 pages, 4 captioned figures, 2 tables, authors from page 12,
published version, figures at
http://aliceinfo.cern.ch/ArtSubmission/node/284
Anisotropic flow of charged hadrons, pions and (anti-)protons measured at high transverse momentum in Pb-Pb collisions at TeV
The elliptic, , triangular, , and quadrangular, , azimuthal
anisotropic flow coefficients are measured for unidentified charged particles,
pions and (anti-)protons in Pb-Pb collisions at TeV
with the ALICE detector at the Large Hadron Collider. Results obtained with the
event plane and four-particle cumulant methods are reported for the
pseudo-rapidity range at different collision centralities and as a
function of transverse momentum, , out to GeV/.
The observed non-zero elliptic and triangular flow depends only weakly on
transverse momentum for GeV/. The small dependence
of the difference between elliptic flow results obtained from the event plane
and four-particle cumulant methods suggests a common origin of flow
fluctuations up to GeV/. The magnitude of the (anti-)proton
elliptic and triangular flow is larger than that of pions out to at least
GeV/ indicating that the particle type dependence persists out
to high .Comment: 16 pages, 5 captioned figures, authors from page 11, published
version, figures at http://aliceinfo.cern.ch/ArtSubmission/node/186
Measurement of charm production at central rapidity in proton-proton collisions at TeV
The -differential production cross sections of the prompt (B
feed-down subtracted) charmed mesons D, D, and D in the rapidity
range , and for transverse momentum GeV/, were
measured in proton-proton collisions at TeV with the ALICE
detector at the Large Hadron Collider. The analysis exploited the hadronic
decays DK, DK, DD, and their charge conjugates, and was performed on a
nb event sample collected in 2011 with a
minimum-bias trigger. The total charm production cross section at TeV and at 7 TeV was evaluated by extrapolating to the full phase space
the -differential production cross sections at TeV
and our previous measurements at TeV. The results were compared
to existing measurements and to perturbative-QCD calculations. The fraction of
cdbar D mesons produced in a vector state was also determined.Comment: 20 pages, 5 captioned figures, 4 tables, authors from page 15,
published version, figures at
http://aliceinfo.cern.ch/ArtSubmission/node/307
Particle-yield modification in jet-like azimuthal di-hadron correlations in Pb-Pb collisions at = 2.76 TeV
The yield of charged particles associated with high- trigger
particles ( GeV/) is measured with the ALICE detector in
Pb-Pb collisions at = 2.76 TeV relative to proton-proton
collisions at the same energy. The conditional per-trigger yields are extracted
from the narrow jet-like correlation peaks in azimuthal di-hadron correlations.
In the 5% most central collisions, we observe that the yield of associated
charged particles with transverse momenta GeV/ on the
away-side drops to about 60% of that observed in pp collisions, while on the
near-side a moderate enhancement of 20-30% is found.Comment: 15 pages, 2 captioned figures, 1 table, authors from page 10,
published version, figures at
http://aliceinfo.cern.ch/ArtSubmission/node/350
Transverse momentum spectra of charged particles in proton-proton collisions at GeV with ALICE at the LHC
The inclusive charged particle transverse momentum distribution is measured
in proton-proton collisions at GeV at the LHC using the ALICE
detector. The measurement is performed in the central pseudorapidity region
over the transverse momentum range GeV/.
The correlation between transverse momentum and particle multiplicity is also
studied. Results are presented for inelastic (INEL) and non-single-diffractive
(NSD) events. The average transverse momentum for is (stat.) (syst.) GeV/ and
\left_{\rm NSD}=0.489\pm0.001 (stat.) (syst.)
GeV/, respectively. The data exhibit a slightly larger than measurements in wider pseudorapidity intervals. The results are
compared to simulations with the Monte Carlo event generators PYTHIA and
PHOJET.Comment: 20 pages, 8 figures, 2 tables, published version, figures at
http://aliceinfo.cern.ch/ArtSubmission/node/390
Neutral pion and meson production in proton-proton collisions at TeV and TeV
The first measurements of the invariant differential cross sections of
inclusive and meson production at mid-rapidity in proton-proton
collisions at TeV and TeV are reported. The
measurement covers the ranges GeV/ and GeV/ for
these two energies, respectively. The production of mesons was measured
at TeV in the range GeV/. Next-to-Leading Order
perturbative QCD calculations, which are consistent with the spectrum
at TeV, overestimate those of and mesons at
TeV, but agree with the measured ratio at
TeV.Comment: 17 pages, 5 captioned figures, 2 tables, authors from page 12,
published version, figures at
http://aliceinfo.cern.ch/ArtSubmission/node/310
Centrality Dependence Of The Pseudorapidity Density Distribution For Charged Particles In Pb-pb Collisions At √snn=2.76tev
7264/Mai61062
J/psi Production as a Function of Charged Particle Multiplicity in pp Collisions at sqrt{s} = 7 TeV
The ALICE collaboration reports the measurement of the inclusive J/psi yield
as a function of charged particle pseudorapidity density dN_{ch}/deta in pp
collisions at sqrt{s} = 7 TeV at the LHC. J/psi particles are detected for p_t
> 0, in the rapidity interval |y| < 0.9 via decay into e+e-, and in the
interval 2.5 < y < 4.0 via decay into mu+mu- pairs. An approximately linear
increase of the J/psi yields normalized to their event average
(dN_{J/psi}/dy)/ with (dN_{ch}/deta)/ is observed
in both rapidity ranges, where dN_{ch}/deta is measured within |eta| < 1 and
p_t > 0. In the highest multiplicity interval with = 24.1,
corresponding to four times the minimum bias multiplicity density, an
enhancement relative to the minimum bias J/psi yield by a factor of about 5 at
2.5 < y < 4 (8 at |y| < 0.9) is observed.Comment: Submitted to Phys. Lett.
Inclusive J/psi production in pp collisions at sqrt(s) = 2.76 TeV
The ALICE Collaboration has measured inclusive J/psi production in pp
collisions at a center of mass energy sqrt(s)=2.76 TeV at the LHC. The results
presented in this Letter refer to the rapidity ranges |y|<0.9 and 2.5<y<4 and
have been obtained by measuring the electron and muon pair decay channels,
respectively. The integrated luminosities for the two channels are L^e_int=1.1
nb^-1 and L^mu_int=19.9 nb^-1, and the corresponding signal statistics are
N_J/psi^e+e-=59 +/- 14 and N_J/psi^mu+mu-=1364 +/- 53. We present
dsigma_J/psi/dy for the two rapidity regions under study and, for the forward-y
range, d^2sigma_J/psi/dydp_t in the transverse momentum domain 0<p_t<8 GeV/c.
The results are compared with previously published results at sqrt(s)=7 TeV and
with theoretical calculations.Comment: 7 figures, 3 tables, accepted for publication in Phys. Lett.
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