Hepatoprotektivno djelovanje ekstrakta biljke Calotropis gigantea na oštećenje jetre štakora tetraklormetanom

Ethanolic extract (50 %) of stems of Calotropis gigantea R. Br. (Asclepiadaceae) at doses of 250 and 500 mg kg1 were studied for hepatoprotective activity in male Wistar rats with liver damage induced using carbon tetrachloride, 2 mL kg-1 twice a week. The protective effect of C. gigantea extract was compared with the standard drug silymarin. Various biochemical parameters such as aspartate amino transferase (AST), alanine amino transferase (ALT), glutathione (GSH), lipid peroxide (LPO), superoxidedismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were evaluated. The results revealed that the C. gigantea extract significantly decreased AST, ALT (p < 0.001) and lipid peroxide (p < 0.01) levels. The antioxidant parameters GSH, GPx, SOD and catalase levels were increased considerably compared to the levels in groups treated with carbon tetrachloride onlyEtanolni ekstrakt (50 %) stabljika biljke Calotropis gigantea R. Br. (Asclepiadaceae) u dozi 250 i 500 mg kg1 testiran je na hepatoprotektivno djelovanje oštećenje jetre mužjaka Wistar štakora inducirano tetraklormetanom, 2 mL kg1 dva puta tjedno. Zaštitni učinak ekstrakta biljke C. gigantea uspoređivan je sa standarnim lijekom silimarinom. Evaluirani su različiti biokemijski parametri kao što su aspartat amino transferaza (AST), alanin amino transferaza (ALT), glutation (GSH), lipidni peroksidi (LPO), superoksiddismutaza (SOD), glutation peroksidaze (GPx) i katalaza (CAT). Rezultati ukazuju da ekstrakt biljke C. gigantea značajno smanjuje koncentracije AST, ALT (p < 0.001) i lipidnih peroksida (p < 0.01). Koncentracije antioksidativnih parametara GSH, GPx, SOD i katalaze bile su značajno povišene u usporedbi sa skupinom tretiranom samo tetraklormetanom

Screening of phytoconstituents and antibacterial effects of leaf & stem of Ailanthus excelsa against E. Coli, S. Aureus, A. Niger & A. Flavus

The research was aimed as screening of phytoconstituents and antibacterial effects of Leaf &amp; Stem of Ailanthus excelsa against E. Coli, S. Aureus, A. Niger &amp; A. Flavus. The plants were collected from Bareilly region, Uttar Pradesh and authentication was done by CIMAP, Lucknow. The collected plant material was dried in shade and ground in the grounder. The dried powdered drug material was extracted by 9 different solvents by cold maceration for 12 hrs at room temperature. Mayer’s, Hager’s tests, Fehling’s Wager’s and Molisch test were used for detection of alkaloids, glycosides, saponins, terpenoids etc. After the extraction was complete, it was tested against various species such as E. coli, S. aureus, S. niger and A. Flavus for its antimicrobial potential. The results indicate that methanolic extract showed highest level of phytoconstituents such as alkaloids, glucosides, saponins, terpenoids, flavonoids, and carbohydrates. Extract showed significant antibacterial potential against all the species tested with. In conclusion, this research suggests, that novel molecules should be identified and isolated responsible for specific pharmacological activity