Plasma Glucose and Lipid Profiles Following High-Fat Diet and Acute Aerobic Exercise

PURPOSE: To examine the effects of a 3-week high-fat (HF) diet on plasma glucose, lipids, and lipoproteins following an acute bout of aerobic exercise in middle-aged men. METHODS: Physically active (non-elite, competitive marathon runners), male participants (N=8, age=39.5±9.9 years) volunteered for the study. Participants maintained their habitual high-carbohydrate (HC) diet (60-70% caloric intake from carbohydrate) prior to switching to the HF diet (70% caloric intake from fat, not exceeding 50g of carbohydrates) for 3 weeks. At the end of each diet trial, participants performed an acute bout of aerobic exercise, which consisted of running at varying paces (personal race paces) on a treadmill for 50 minutes (split into 5, 10-minute periods with 2 minutes of rest in between). Following a 20-minute recovery from the treadmill exercise, participants additionally ran a 5-km time trial (average run time = 23.69±2.41 minutes) on an outdoor road course. Overnight fasting blood samples were collected before and 24 hours after exercise for the HC and HF diet trial to analyze glucose, triglyceride (TG), total cholesterol (TC), high-density lipoprotein-cholesterol (HDL-C), lipoprotein(a), very low-density lipoprotein cholesterol (VLDL-C), low-density lipoprotein-cholesterol (LDL-C). The data were analyzed using a two-way ANOVA [2 (diet: HC and HF) X 2 (time: pre and post-exercise)]. Any significant interactions were further analyzed using a Tukey’s post-hoc test. A p-value was set at \u3c 0.05. RESULTS: A glucose level was higher (p=0.046) in the HC diet (96.81±2.45 mg/dL) than the HF diet (89.6±2.45 mg/dL). As compared with the HC diet, the HF diet showed a higher level of TC (142.58±4.75 vs. 171.71±4.75 mg/dL, p=0.001), HDL-C (49.26±3.01 vs. 58.58±3.01 mg/dL, p=0.037), and LDL-C (91.51±4.91 vs. 111.20±4.91 mg/dL, p=0.008), respectively. TG significantly decreased (p=0.03) from 65.68±5.93 to 38.46±5.93 mg/dL at 24 hours of post-exercise. CONCLUSION: The 3-weeks of HF diet modestly increased plasma lipids and lipoproteins within the desirable range. Implementing a relatively short-term HF diet does not appear to significantly elicit negative cardiovascular disease risk markers in non-elite, healthy middle-aged male runners. However, it is strongly recommended for future studies to investigate the safety and beneficial effects of a long-term HF diet on cardiovascular disease risk factors in a variety of population including the untrained

Beyond rescue: Implementation and evaluation of revised naloxone training for law enforcement officers

ObjectiveThis study describes the implementation and evaluation of revised opioid overdose prevention and education of naloxone training for law enforcement officers (LEOs) that added: (1) a recovery testimony and (2) the process for deputy‐initiated referrals postnaloxone administration.Design and SampleEvaluation regarding the naloxone training included a pre‐ and postopioid overdose knowledge surveys (N = 114) and subsequent 1‐year postnaloxone training outcomes.ResultsPre‐ and posttest scores for all knowledge outcome measures were statistically significant (p < .001) with favorable comments pertaining to the recovery testimony. Out of 31 individuals who received naloxone, 6 individuals (19.4%) continue to be in treatment or received some treatment services. The most common symptoms reported were unconsciousness/unresponsiveness (40.5%), abnormal breathing patterns (24.3%), and blue lips (16.2%). The majority of the calls (65.6%) were to a residential area, and the time for naloxone revival ranged <1–10 min (M = 3.48; SD = 2.27).ConclusionAs nearly 20% of individuals sought treatment after a LEO‐initiated referral, it is recommended that other agencies consider the referral process into the training. Future research will investigate the impact of the recovery testimony in reducing the stigma of addiction.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/139953/1/phn12365_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/139953/2/phn12365.pd

Using a private 5G network to support the international broadcast of the coronation of HM King Charles III

Wireless cameras for news contribution feeds regularly use “bonded-cellular” devices, which connect to and split the encoded video across multiple public mobile network SIMs. However, in high demand density environments with large crowds, the public networks can quickly become saturated and unable to sustain the necessary bitrates to support high-definition video. To overcome this and provide uncontested wireless connectivity, the largest pop-up 5G standalone non-public (private) network of its type was deployed outside Buckingham Palace and along The Mall to Admiralty Arch to support news contributions for domestic and foreign broadcasters at the Coronation of HM King Charles III, without changing the contribution workflow. (This paper first appeared in the Proceedings of the 2024 NAB Broadcast Engineering and Information Technology Conference, and is reprinted with permission. https://nabpilot.org/beitc-proceedings/

African Linguistics in Central and Eastern Europe, and in the Nordic Countries

Non peer reviewe

Retrospective evaluation of whole exome and genome mutation calls in 746 cancer samples

Funder: NCI U24CA211006Abstract: The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) curated consensus somatic mutation calls using whole exome sequencing (WES) and whole genome sequencing (WGS), respectively. Here, as part of the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium, which aggregated whole genome sequencing data from 2,658 cancers across 38 tumour types, we compare WES and WGS side-by-side from 746 TCGA samples, finding that ~80% of mutations overlap in covered exonic regions. We estimate that low variant allele fraction (VAF < 15%) and clonal heterogeneity contribute up to 68% of private WGS mutations and 71% of private WES mutations. We observe that ~30% of private WGS mutations trace to mutations identified by a single variant caller in WES consensus efforts. WGS captures both ~50% more variation in exonic regions and un-observed mutations in loci with variable GC-content. Together, our analysis highlights technological divergences between two reproducible somatic variant detection efforts

5-km Time Trial Reliability of a Non-Motorized Treadmill and Comparison of Physiological and Perceptual Responses Versus a Motorized Treadmill.

This study examined the reliability of running performance across 3 nonmotorized treadmill (NMT) 5-km time trials (TTs) and physiological, gait, and perceptual differences at a 5-km pace for both NMT and motorized treadmills (MTs). Ten male runners experienced in road racing who had never run on an NMT completed 3 TT to establish personal best 5-km pace. In a later session, participants ran at this pace for 5 minutes on the NMT while metabolic, gait, and perceptual measures were recorded and then ran at outdoor 5-km personal best pace on an MT at 1% grade (counter-balanced crossover design). Intraclass correlation (ICC = 0.95) between the TT1 and TT2 was strong but improved between TT2 and TT3 (ICC = 0.99) with considerable reduction in variability. Nonmotorized treadmill resulted in a 24% slower pace (10.6 6 1.5 vs. 13.9 6 2.6 km$h21; p , 0.001), shorter stride length (1.02 6 0.10 vs. 1.27 6 0.18 m; p , 0.001), and decreased cadence (175 6 12 vs. 181 6 13 steps per$minute; p = 0.01). However, V_ O2, respiratory exchange ratio (RER), lactate concentration, and heart rate did not differ between modalities (NMT = 3.4 6 0.4 L$min21, 0.96 6 0.04, 6.9 6 3.7 mmol, 172 6 10 b$min21; MT = 3.4 6 0.5 L$min21, 0.96 6 0.04, 5.7 6 3.4 mmol, 170 6 10 b$min21). rate of perceived exertion (RPE) for legs, breathing, and overall did not differ between treadmill types. A familiarization session should be included for TT using NMT. Other than gait and pace characteristics similar responses were elicited by both treadmills when running at 5-km pace. However, with these considerations, NMT TT of 4-km might be more appropriate in matching MT 5-km TT duration without altering physiological responses significantly

Five-kilometer time trial reliability of a nonmotorized treadmill and comparison of physiological and perceptual responses vs. A motorized treadmill

© 2017 National Strength and Conditioning Association. This study examined the reliability of running performance across 3 nonmotorized treadmill (NMT) 5-km time trials (TTs) and physiological, gait, and perceptual differences at a 5-km pace for both NMT and motorized treadmills (MTs). Ten male runners experienced in road racing who had never run on an NMT completed 3 TT to establish personal best 5-km pace. In a later session, participants ran at this pace for 5 minutes on the NMT while metabolic, gait, and perceptual measures were recorded and then ran at outdoor 5-km personal best pace on an MT at 1% grade (counter-balanced crossover design). Intraclass correlation (ICC = 0.95) between the TT1 and TT2 was strong but improved between TT2 and TT3 (ICC = 0.99) with considerable reduction in variability. Nonmotorized treadmill resulted in a 24% slower pace (10.6 ± 1.5 vs. 13.9 ± 2.6 km-h21; p \u3c 0.001), shorter stride length (1.02 ± 0.10 vs. 1.27 ± 0.18 m; p \u3c 0.001), and decreased cadence (175 ± 12 vs. 181 ± 13 steps Per minute; p = 0.01). However, VO2, respiratory exchange ratio (RER), lactate concentration, and heart rate did not differ between modalities (NMT = 3.4 ± 0.4 L min-1, 0.96 ± 0.04, 6.9 ± 3.7 mmol, 172 ± 10 b min-1; MT = 3.4 ± 0.5 L-min21, 0.96 ± 0.04, 5.7 ± 3.4 mmol, 170 ± 10 b-min21). rate of perceived exertion (RPE) for legs, breathing, and overall did not differ between treadmill types. A familiarization session should be included for TT using NMT. Other than gait and pace characteristics similar responses were elicited by both treadmills when running at 5-km pace. However, with these considerations, NMT TT of 4-km might be more appropriate in matching MT 5-km TT duration without altering physiological responses significantly

Five-kilometer time trial reliability of a nonmotorized treadmill and comparison of physiological and perceptual responses vs. A motorized treadmill

© 2017 National Strength and Conditioning Association. This study examined the reliability of running performance across 3 nonmotorized treadmill (NMT) 5-km time trials (TTs) and physiological, gait, and perceptual differences at a 5-km pace for both NMT and motorized treadmills (MTs). Ten male runners experienced in road racing who had never run on an NMT completed 3 TT to establish personal best 5-km pace. In a later session, participants ran at this pace for 5 minutes on the NMT while metabolic, gait, and perceptual measures were recorded and then ran at outdoor 5-km personal best pace on an MT at 1% grade (counter-balanced crossover design). Intraclass correlation (ICC = 0.95) between the TT1 and TT2 was strong but improved between TT2 and TT3 (ICC = 0.99) with considerable reduction in variability. Nonmotorized treadmill resulted in a 24% slower pace (10.6 ± 1.5 vs. 13.9 ± 2.6 km-h21; p \u3c 0.001), shorter stride length (1.02 ± 0.10 vs. 1.27 ± 0.18 m; p \u3c 0.001), and decreased cadence (175 ± 12 vs. 181 ± 13 steps Per minute; p = 0.01). However, VO2, respiratory exchange ratio (RER), lactate concentration, and heart rate did not differ between modalities (NMT = 3.4 ± 0.4 L min-1, 0.96 ± 0.04, 6.9 ± 3.7 mmol, 172 ± 10 b min-1; MT = 3.4 ± 0.5 L-min21, 0.96 ± 0.04, 5.7 ± 3.4 mmol, 170 ± 10 b-min21). rate of perceived exertion (RPE) for legs, breathing, and overall did not differ between treadmill types. A familiarization session should be included for TT using NMT. Other than gait and pace characteristics similar responses were elicited by both treadmills when running at 5-km pace. However, with these considerations, NMT TT of 4-km might be more appropriate in matching MT 5-km TT duration without altering physiological responses significantly